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Xi-Yang Li,Qian-Lin Chen,Min Yang,Ya-Nan Li,Jing-Bo Ma 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.3
The lithium titanium spinel Li4Ti5O12 has attracted more and more attention as anode materials applied in lithium ion batteries. Li4Ti5O12 material has been found to be able to intercalate lithium ions without deformation of the lattice. However, compared with graphite and other anode materials, the low conductivity of Li4Ti5O12 restricts its charging and discharging rate. Doping is deemed to be a businesslike method to enhance ionic and electronic conductivity of Li4Ti5O12. This paper reviews the effects of Li4Ti5O12 with different doping ions on different crystal lattice states. And it has been found by a summary that the doping objective of doping ions at Li4Ti5O12 is also different. Moreover, the applications of ion doping in different fields of Li4Ti5O12 are prospected.
Ya-Nan Zhang,Peng He,Jian-Ping Xue,Qing Guo,Xiu-Yun Zhu,Li-Ping Fang,Jin-Bu Li 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
The beet armyworm, Spodoptera exigua Hübner (Lepidoptera: Noctuidae), is a key pest of various agricultural crops in many countries throughout the world. The pest requires extensive use of pesticides and field-evolved resistances to conventional insecticides in China and other countries. Pinellia ternata Breit is native to the eastern part of Asia and foundmainly in China,which has been used in traditional Chinese medicines for N1000 years, but few studies have focused on the insecticidal activity of the P. ternate. In order to find natural products that could be used to control the pest in an safe, efficient and ecofriendlymanner,we first time analyzed the components of the anhydrous ethanolic extracts fromthe tubers of Pinellia ternate by using GC–MS method, and then investigated the insecticidal activities and biochemical mechanisms of the extracts against S. exigua. The result of GC–MS showed that 2-Methoxy-4-vinylphenolmay be an insecticidal active component, and we also found the extracts had notable insecticidal activity and disturbed the regular metabolismof S. exiguamainly through altering the activities of detoxification enzymes, digestive enzymes and protective enzymes. These properties suggest that the anhydrous ethanolic extracts from P. ternate can serve as a potential, efficient and ecofriendly S. exigua-control biopesticide.
Ya-Nan Zhang,Ji-FangMa,Lu Xu,Zhi-PingDong,Ji-Wei Xu,Meng-Ya Li,Xiu-Yun Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1
UDP-glucuronosyltransferase (UGT) genes,which belong to an ancient gene family and play very important roles in all organisms, encode extracellularly secreted proteins that are involved in the transfer of glycosyl residues fromactivated nucleotide sugars to acceptor hydrophobicmolecules (aglycones). Athetis lepigone is an important polyphagous pest worldwide, and since 2011 it has become one of the major maize pests in North China. However, there have been no studies on pesticides for the effective control of this pest. In this study,we identified 23 putative UGT genes in A. lepigone by analysing previous antennal transcriptomic data. Phylogenetic analysis showed that all of the AlUGTs are distributed within 11 of 14 insect UGT sub-families. Tissue expression analysis revealed that N70% of AlUGTs were primarily expressed in adult antennae, of which three (AlUGT33AD1, AlUGT40F6 and AlUGT40L4) and four (AlUGT33B18, AlUGT33F10, AlUGT40Q3 and AlUGT41D3) displayed male-biased and female-biased expression, respectively. SomeAlUGTs, however, had higher expression levels in non-antennal tissues. Our study is the first to identify UGT genes in A. lepigone,which will help us to elucidate the diverse functions of these genes, and ultimately provide potential targets that will facilitate the development of efficient and environmentally friendly pesticides against A. lepigone.
Ya-Jie Li,Rui Mi,Nan Meng,Zhi-Xin Wen,Xue-Jun Li,Mo Chen,Yan-Qun Liu,Shu-Ying Li 한국응용곤충학회 2013 Journal of Asia-Pacific Entomology Vol.16 No.3
Sans-fille (SNF) is the Drosophila homologue ofmammalian general splicing factors U1A and U2B″, and plays an important role in sex determination in Drosophilamelanogaster. In this study, the snf gene fromAntheraea pernyi (Lepidoptera: Saturniidae), an economically important insect, was isolated and characterized. The obtained 925 bp cDNA sequence contains an open reading frame of 669 bp encoding a polypeptide of 222 amino acids,showing 78% sequence identity to that from D. melanogaster. A database search revealed that SNF protein homologs are present in many animals, including invertebrates and vertebrates, with more than 70% amino acid sequence identities, suggesting that they were highly conserved during the evolution of animals. Phylogenetic analysis revealed that A. pernyi SNF was closely related to Bombyx mori SNF. Quantitative real-time PCR (qRT-PCR) analysis showed that the A. pernyi snf gene was transcribed during five larval developmental stages,and in six tested tissues (ovaries, testes, silk glands, fat body, integument, and hemolymph),with the most abundance determined in the gonads (ovaries or testes). Investigation of expression changes throughout embryonic development indicated that A. pernyi snfmRNAwas expressed at a lowlevel fromdays 0 to 4, and reached amaximum level at day 10, but decreased to a low level before hatching. These results suggest that the product of the snf gene may play important roles in the development of A. pernyi.
New criteria on finite-time stability of nonautonomous systems with delay and disturbance
Ya-Nan Li,Yuan-Gong Sun 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10
This paper is concerned with the problem of finite-time stability for nonautonomous systems subject to timevarying delay and disturbance. Based on the piecewise estimate of the time-varying system matrices, we establish a new explicit criterion in terms of multiple linear matrix inequalities guaranteeing that the state trajectory does not exceed a certain threshold over a pre-specified finite-time interval. The main result of this paper generalizes and improves some existing results in the literature, and can also be extended to the more general nonlinear case. Finally, a numerical example is worked out to illustrate the effectiveness of the proposed method.
Ya-Nan Zhang,Zhao-Qun Li,Xiu-Yun Zhu,Jia-Li Qian,Zhi-Ping Dong,Lu Xue,Peng He 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4
Some of the metabolic enzyme carboxylesterases (CXEs) belong to the odorant-degrading enzymes (ODEs) family in insect species, and these play a key role in the degradation of acetate sex pheromones and host plant volatiles. Athetis lepigone is one of the most important agricultural insect pests in the world, can damage> 30 species of host plants, and has caused serious declines in the yield of summer corn in North China since 2011. According to our previous studies, the sex pheromone component of the pest is a binary blend of Z7–12:OAc and Z9–14:OAc at a ratio of 3:7. However, there are no reports regarding the degradation mechanism for these two sex pheromones. Herein, we firstly identified 20 candidate CXE genes in A. lepigone using our previous adult antennal RNA-seq data. Then, we constructed a phylogenetic tree and further conducted tissue distribution analyses to determine the possible functions of these genes. Our results showed that some AlepCXEs displayed adult antennae- predominant, male antennae-biased, or leg/wing-biased expression, indicating these AlepCXEs may have distinct physiological functions and play distinct roles in the degradation of sex pheromones, host plant volatiles, and/or other xenobiotics. These findings will help us to elucidate the exact functions of these genes in the future, and also provide possible target genes for the prevention and control of A. lepigone.
Ya-Nan Zhang,Ji-FangMa,Liang Sun,Zhi-PingDong,Zhao-Qun Li,Xiu-Yun Zhu,YiWang,LuWang,Dao-Gui Deng,Jin-Bu Li 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
Chemosensory receptors play central roles in insect behavior and can potentially be used as new targets for pest control. Athetis lepigoneMöschler (Lepidoptera: Noctuidae) is a polyphagous insect pest found in many countries in Europe and Asia. However, systemic identification of the chemosensory receptors in the antennae has not been reported. In the present study, we obtained the antennal transcriptome of A. lepigone using Illumina sequencing technology and identified 80 candidate chemosensory receptors, including 61 transcripts encoding for odorant receptors (ORs) and 19 for ionotropic receptors (IRs). This result is similar to that reported for other lepidopteran species, suggesting that our approach successfully identified ORs and IRs of A. lepigone. RT-PCR was used to determine the sex distribution patterns of all OR and IR genes. Some OR and IR genes showed male- or femalespecific/ predominant expression. Our results help understand the functions of chemosensory receptor genes in A. lepigone as well as other insects, and suggest that these genes could be potential targets for developing environment-friendly behavioral antagonists and pesticides against A. lepigone.
Ya Nan Zhang,Jin-Bu Li,Peng He,Liang Sun,Zhao-Qun Li,Li-Ping Fang,Zhan-Feng Ye,Dao-Gui Deng,Xiu-Yun Zhu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4
Carboxylesterases (CXEs) belong to a family of metabolic enzymes that are widely distributed in insects and other organisms and can rapidly degrade the components of sex pheromones and plant volatiles with an acetate functional group. The common cutworm, Spodoptera litura, is an important agricultural pest around the world, causing vast economic losses every year. The female sex pheromones of S. litura comprise four acetates, Z9, E11-14:OAc; Z9, E12-14:OAc; Z9-14:OAc; and E11-14:OAc, but the degradation mechanisms of these components are not well understood. By analysing previously obtained transcriptomic data of the sex pheromone glands,we identified a total of 24 putative CXE genes in S. litura. Gene expression patterns and phylogenetic analysis revealed 5 genes with antennae-specific or biased expression, and clusteredwith genes showed involvement in the degradation of sex pheromones or other detoxification in other insects. SlitCXE10was expressed specifically in the antennae of both sexes, and SlitCXE14, 17, 19, and 21 had high antenna biased expression. Interestingly, RT-PCR and qPCR tests indicated that SlitCXE24 had significantly higher expression in PG than in other tissue, and that it could be a potential candidate gene for sex pheromone degradation in PG. This study is the first to provide solid background information for the further elucidation of sex pheromone degradation, and ultimately provides potential targets for the disruption of sexual communication in S. litura for new pest management.