Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways

Xue, Xia,Yu, Jin-Long,Sun, De-Qing,Kong, Feng,Qu, Xian-Jun,Zou, Wen,Wu, Jing,Wang, Rong-Mei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Curcumin, a polyphenol compound derived from the rhizome of the plant Curcuma longa L. has been verified as an anticancer compound against several types of cancer. However, understanding of the molecular mechanisms by which it induces apoptosis is limited. In this study, the anticancer efficacy of curcumin was investigated in human gastric adenocarcinoma SGC-7901 cells. The results demonstrated that curcumin induced morphological changes and decreased cell viability. Apoptosis triggered by curcumin was visualized using Annexin V-FITC/7-AAD staining. Curcumin-induced apoptosis of SGC-7901 cells was associated with the dissipation of mitochondrial membrane potential (MMP) and the release of cytochrome c into the cytosol. Furthermore, the down-regulation of Bcl-2 and up-regulation of Bax that led to the cleavage of caspase-3 and increased cleaved PARP was observed in SGC-7901 cells treated with curcumin. Therefore, curcumin-induced apoptosis of SGC-7901 cells might be mediated through the mitochondria pathway, which gives the rationale for in vivo studies on the utilization of curcumin as a potential cancer therapeutic compound.

The Role of Foreign Direct Investment on Ecological Footprint of the ASEAN-5 Nations

Xuexia Zheng,UTAI UPRASEN 한국동남아학회 2022 동남아시아연구 Vol.32 No.3

This study investigates the effect of foreign direct investment (FDI) on ecological footprint in 5 ASEAN countries: Indonesia, Malaysia, the Philippines, Singapore, and Thailand. We implement the autoregressive distributed lag (ARDL) model to acquire the outcomes of short-run and long-run coefficients using annual data during 1980 to 2018. Furthermore, Toda-Yamamoto Granger causality test is employed to assure the robustness of our study. The empirical findings reveal that positive effects of FDI on ecological footprint are detected in Indonesia and Malaysia, while Thailand has negative connection between FDI and the ecological footprint. However, there is no significant impact of FDI on ecological footprint in the Philippines and Singapore. In addition, the results from control variables indicate that GDP per capita shows positive impact on ecological footprint in Indonesia, whereas, it has a negative effect in Malaysia, Singapore and Thailand. The exports have no impact on ecological footprint in all 5 ASEAN countries. Nonetheless, energy consumption exhibit positive association with ecological footprint for all 5 ASEAN members.

Impact of Foreign Direct Investment on Renewable Energy Consumption in ASEAN Countries

Xuexia Zheng,UTAI UPRASEN 한국외국어대학교 동남아연구소 2022 東南亞硏究 Vol.32 No.1

The climate change raises the global concern on utilizing of eco-friendly energy, the renewable energy. The current evidences indicate that consumption of renewable energy in the ASEAN countries is low. Consequently, this article scrutinizes the factors affecting renewable energy consumption in the ASEAN 5 countries by focusing the roles of foreign direct investment (FDI). The autoregressive distributed lag (ARDL) model is employed, using the data from 1990 to 2019. In addition, to increase the robustness of our work, the Toda-Yamamoto Granger causality test is performed. The empirical findings show that there are positive associations between FDI and renewable energy consumption in Indonesia, Malaysia, the Philippines, and Singapore. Moreover, GDP per capita positively contributes to renewable energy consumption in Malaysia, but the negative effect is found in Indonesia and Thailand. While the positive impact of export is observed in Malaysia and Thailand, it shows negative one in Singapore. Lastly, financial development has negative relationship with renewable energy consumption in Malaysia and the Philippines.

Determinants of Foreign Direct Investment in Jiangsu Province, China

( Xuexia Zheng ),( Utai Uprasen ) 부경대학교 글로벌지역학연구소 2021 Journal of Global and Area Studies(JGA) Vol.5 No.2

China has turned to be one of the world’s most significant foreign direct investment (FDI) recipients, with FDI stock second only to the United States. Even though total FDI in China is substantially large, the spatial distribution of FDI is extremely uneven. The eastern coastal regions have always been important areas of China to receive FDI. Among the eastern coastal areas, Jiangsu province is consistently ranked second behind Guangdong province in terms of total FDI in China. However, FDI inflows of Jiangsu province surpassed Guangdong province for the first time in 2003. Although Jiangsu province has become a remarkable destination of FDI, the current FDI growth rate has started reducing. Therefore, it is crucial to investigate the determinants of FDI in Jiangsu province. Consequently, this study examines the determinants of FDI in Jiangsu province by employing an autoregressive distributed lag (ARDL) model with annual data during 1985-2019. In addition, we apply the Toda-Yamamoto Granger causality test to increase the robustness of our empirical results. The findings show that FDI in Jiangsu province is determined by average wage, education, and per capita GDP. Consequently, Jiangsu authorities should implement a policy to ensure a low wage rate and promote education in order to attract more FDI. In addition, the policy on economic development is needed to promote inward FDI in Jiangsu.

외국인 직접 투자에 영향을 미치는 요인에 대한 실증분석: 중국 광둥성의 사례

정설하 ( Xuexia Zheng ),( Utai Uprasen ) 국제지역학회 2021 국제지역연구 Vol.25 No.3

1970년대 말부터 중국은 개방과 경제 개혁 정책을 시행하여 다양한 국가에서 유입되는 외국인 직접 투자(FDI)의 중요한 목적지가 되어왔다. 그 중 광둥성은 이러한 정책 시행에 주도적인 역할을 하여 중국 본토의 31개 성 및 지자체 중 FDI를 가장 많이 받는 곳이 되었다. 대부분의 기존 연구는 국가 차원에서 중국의 FDI 유입의 결정 요인을 연구하는데 집중하였고 지역 차원의 연구는 등한시 되었다. 하지만, 현재의 연구결과에 따르면 광둥성의 총 FDI 유입은 시간이 지남에 따라 증가했지만 2003년 이후 FDI 비중이 현저히 감소한 것으로 나타났다. 이러한 감소를 토대로 광둥성의 FDI 유입 결정 요인을 살펴보는 것은 매우 중요하다. 본 연구는 1990년부터 2019년까지의 연간 데이터를 ARDL(Autoregressive Distributed lag) 모델을 사용하여 광동성의 FDI 유입에 영향을 미치는 요인을 분석했다. 추가적으로 Toda-Yamamoto Granger 인과성 검정을 사용하여 견고성을 높였다. 결과적으로 광둥성의 FDI는 평균 임금, 1인당 GDP, 기반 시설 및 실질 실효 환율에 의해 결정되었고 무역 개방성은 미미했다. 이러한 실증적 발견은 Toda-Yamamoto Granger 인과관계 검정으로 재검토되어 뒷받침하였다. Since the end of 1970s, China has implemented opening up and economic reform policies. China has become a significant destination of inward foreign direct investment (FDI) from various countries. Guangdong province has taken a leading role on such policy implementation. Consequently, it turned to be the largest recipient of FDI among 31 provinces and municipalities of Chinese mainland. Most of the existing works investigate the determinants of inward FDI of China at a country level. The province-level research is scarce. Furthermore, the current evidences indicate that although the total FDI inflows of Guangdong have increased over time, its FDI share has decreased remarkably since 2003. Hence, it is very crucial to examine the determinants of FDI inflows of Guangdong province. As a result, our research scrutinizes the factors affecting inward FDI in the case of Guangdong province by using autoregressive distributed lag (ARDL) model with annual data from 1990 to 2019. Additionally, we employ the Toda-Yamamoto Granger causality test to increase the robustness of our empirical results. The findings suggest that inward FDI of Guangdong province is determined by average wage, per capita GDP, infrastructure and real effective exchange rate. Trade openness is insignificant. These empirical findings are also supported by the Toda-Yamamoto Granger causality test.

Vehicle Scheduling Optimization Based on Chaos Ant Colony Algorithm in Emergency Rescue

Lingxia Liu,Xuexia Gao,Qiang Song 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.2

Aiming to the demand and characteristics of vehicle scheduling optimization problem in emergency rescue, this paper establishes a multi-objective optimization model, which takes minimizing variable bidirectional distance, path risk and cost as the optimization target. To avoid the prematurely falling into local optimization of ant colony system(ACS)algorithm, and to improve the algorithm adaptability, computational efficiency and the quality of optimal solution, this paper proposed and realized a chaos-based improved ant colony system algorithm, which can overall updates the chaos disturbance to pheromone. Simulation results show that the algorithm is feasible, which can well meet the demand of vehicle scheduling optimization in emergency rescue.

Identification and phylogeny of five male-specific lethal genes in the silkworm Bombyx mori

Wenbin LIU,Yong ZHANG,Xuexia MIAO,Yongping Huang 한국곤충학회 2008 Entomological Research Vol.38 No.-

In the fruit fly (Drosophila melanogaster) dosage compensation equalization of X-linked genes between the sexes is achieved via a complex that is termed the compensasome, which is composed of at least five male-specific lethal (msl) genes and two non-coding RNAs. In the present study, we cloned all five homologs (termed the msls) from the silkworm, Bombyx mori, in which the issue of dosage compensation remains controversial. Data mining and robust phylogenetic analysis revealed that msls are ubiquitously present in genomes, from insects to mammals, with unknown biological functions. However, the five genes seem to have evolved at different times due to gene duplication. Real-time reverse transcriptase-polymerase chain reaction experiments demonstrated that in the silkworm, some Z-linked genes are expressed at higher levels in females than in males while others are expressed at lower levels in females than in males, which suggests that the msl genes in the silkworm and fruit fly may have different roles. We discussed the relationship of phylogeny between msl and sex-lethal.

Studies on the Hereditary Properties of SSR Marker in Silkworm (Bombyx mori L.)

Li Muwang,Li Minghui,Miao Xuexia,Lu Cheng,Huang Yongping Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.11 No.1

Two BC1 group, c108 $\times$ (p50 $\times$ c108) and p50 $\times$ (p50 $\times$ c108), one group of F$_{2}$ progeny, (p50 $\times$ c108) F$_{2}$ ,and 3 SSR markers, F10539, FlO626 and FlO618 were used to test the hereditary properties of SSR markers in silkworm. FI0539, FlO626 were proved to be linkage, and FlO618 was proved to be independent to those two markers. According to Mendel's law, the recombinant value between F10539, FlO626 was calculated in all of these groups, and they were 8.55$\%$ (c108BC1), 8.02$\%$ (p50BC1) and 7.81 $\%$ (F$_{2}$) respectively. There was dominant difference among the crossing-over value using paired-samples tests by SPSS 10.0 software. This research proved that SSR markers were co-dominant in B. mori too, and F 2 progeny could be used to construct SSR linkage map although B. mori lacked of crossing over in females.

A core–shell polymeric–inorganic hybrid nanocomposite system for MRI-visible gene delivery application in cancer immunotherapy

Jiali Cai,Guochuang Chen,Rongrong Jin,Changhui Deng,Shihui Huang,Xuexia Yuan,Gengjia Chen,Jing Zhao,Zhiyong Wang,Hua Ai 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.76 No.-

Due to the tumor immune escape mechanism, strategies like the utilization of bispecific antibodies wereused to assist immunotherapy. Herein a visible polymer–inorganic hybrid gene vehicle had beenprepared to deliver anti-EpCAM/anti-CD3 encoding minicircle DNA into normal cells thus thesetransfected cells effectively secreted bispecific antibody with bioactivity. In the experiments, thiscompound system exhibited excellent biocompatibility, high transfection efficiency and ultrasensitiveimaging capacity. Furthermore, the transfected cells could be detected under the MR imaging system andthe gene product showed an outstanding immune effect. In conclusion, this nanocomposite showedsynergistic advantages in gene delivery and non-invasive MR imaging.

MiR-183-5p Promotes Proliferation, Metastasis and Angiogenesis in Breast Cancer Cells through Negatively Regulating Four and a Half LIM Protein 1

Yi Li,Qing'an Zeng,Jiliang Qiu,Ting Pang,Fenglian Ye,Lin Huang,Xuexia Zhang 한국유방암학회 2020 Journal of breast cancer Vol.23 No.4

Purpose: Four and a half LIM protein 1 (FHL1) is involved in breast cancer (BC) development, but the regulatory mechanism involved remain unclear. In the present study, we examined the role of FHL1 in BC development. Methods: The expression of FHL1, miR-183-5p, and miR-96-5p in BC tissues was analyzed using StarBase analysis. FHL1 expression in BC tissues, a normal human breast epithelial cell line, and BC cell lines was detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The relationship between FHL1 and miR-183-5p/miR-96-5p was analyzed via Pearson's rank correlation, TargetScan, and a dual-luciferase reporter assay. BT549 and MDA-MB-231 cells were transfected with either FHL1 and miR-183-5p mimics, or siFHL1 and a miR-183-5p inhibitor, respectively. The viability, colony number, migration, invasion, and tube length of BT549 and MDA-MB-231 cells were examined using cell counting kit-8, colony formation, wound-healing, Transwell, and tube formation assays, respectively. The levels of FHL1, vascular endothelial growth factor (VEGF), p53, E-cadherin, N-cadherin, and vimentin were quantified using western blotting and qRT-PCR. Results: FHL1 expression was downregulated in BC tissues and cells, whereas miR-183-5p and miR-96-5p were upregulated in BC tissues (negative correlation with FHL1 expression). FHL1 overexpression inhibited the viability, colony number, migration, and invasion of BC cells and the expression of VEGF, N-cadherin, and vimentin, and increased the expression of FHL1, p53, and E-cadherin in BT549 cells. Furthermore, a miR-183-5p mimic reversed these effects of FHL1 overexpression, whereas FHL1 silencing caused opposite results to those observed in MDA-MB-231 cells; however, this was reversed by a miR-183-5p inhibitor. Conclusion: Our study suggests that miR-183-5p promotes cell proliferation, metastasis, and angiogenesis by negatively regulating FHL1 in BC.