LncRNA A2M-AS1 lessens the injury of cardiomyocytes caused by hypoxia and reoxygenation via regulating IL1R2

Xue‑Lian Song,Fei‑Fei Zhang,Wen‑Jing Wang,Xin‑Ning Li,Yi Dang,Ying‑Xiao Li,Qian Yang,Mei‑Jing Shi,Xiao‑Yong Qi 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Myocardial ischemia and reperfusion injury (MI/RI) is a complex pathophysiological process, which can lead to severe myocardial injury. The long noncoding RNA alpha-2-macroglobulin antisense RNA 1 (A2M-AS1) has been revealed to be abnormally expressed in MI, However, its function in MI and the potential mechanism are still unclear. Objective: To evaluate the functional role of A2M-AS1 in hypoxia/reoxygenation (H/R)-induced neonatal cardiomyocytes and its potential molecular mechanism. Methods: Dataset GSE66360 was obtained from GEO database for analyzing the RNA expression of A2M-AS1 and interleukin 1 receptor type 2 (IL1R2). KEGG pathway enrichment analysis of the genes that co-expressed with A2M-AS1 was performed. Human neonatal cardiomyocytes were subjected to H/R to construct in vitro models. QRT-PCR and Western blot were adopted to test the levels of mRNA and protein. The viability and apoptosis of cardiomyocytes were tested by CCK-8 and flow cytometry assays, respectively. Results: The expression of A2M-AS1 was notably downregulated in H/R-treated cardiomyocytes. Overexpression of A2M-AS1 can notably enhance the cell viability of H/R-damaged cardiomyocytes, whereas knockdown of A2M-AS1 showed the opposite outcomes. Besides, a negative correlation was showed between A2M-AS1 and IL1R2 expression. In H/R-treated cardiomyocytes, overexpression of IL1R2 weakened the promoting proliferation and anti-apoptosis effects caused by overexpressing A2M-AS1, however, IL1R2-knockdown abolished the anti-proliferation and pro-apoptosis effects caused by silencing A2M-AS1. Conclusion: This study demonstrates the potential regulatory role of A2M-AS1/ IL1R2 axis in cardiomyocytes suffered from H/R, and provides insight into the protection of MI/RI.

Task Allocation of Intelligent Warehouse Picking System based on Multi-robot Coalition

( Fei Xue ),( Hengliang Tang ),( Qinghua Su ),( Tao Li ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.7

In intelligent warehouse picking system, the allocation of tasks has an important influence on the efficiency of the whole system because of the large number of robots and orders. The paper proposes a method to solve the task allocation problem that multi-robot task allocation problem is transformed into transportation problem to find a collision-free task allocation scheme and then improve the capability of task processing. The task time window and the power consumption of multi-robot (driving distance) are regarded as the utility function and the maximized utility function is the objective function. Then an integer programming formulation is constructed considering the number of task assignment on an agent according to their battery consumption restriction. The problem of task allocation is solved by table working method. Finally, simulation modeling of the methods based on table working method is carried out. Results show that the method has good performance and can improve the efficiency of the task execution.

Case Study of the Impact of Packing Regulations and Technical Standards on Expert Trade

Xue-Fei Du,Hyun-Sook Cho 강원대학교 경영경제연구소 2021 Asia-Pacific Journal of Business Vol.12 No.3

technical standard barrier on export trade. Design/methodology/approach - This study analyzed cases related to packing in major countries such as US, EU and so on. It is also show the example of export volume changed based on PRTS. Findings - First, the role of packaging and the reason why countries issued relevant packaging provisions were introduced. Then, the impacts of PRTS on export trade were expounded. And next, we focused on investigating some PRTS related cases in export trade and gave an example of export volume changed based on PRTS. Finally, we produced implications for business leaders to aware of the importance of PRTS in export. Research implications or Originality This study provide PRTS information to traders. Trade pratitioners can respond to PRTS related regulations, actively develop PRTS technology, and take relavant to deal with PRTS barriers to eliminate or weaken the adverse effects of PRTS on export trade.

Nonsurgical correction of a severe anterior deep overbite accompanied by a gummy smile and posterior scissor bite using a miniscrew-assisted straight-wire technique in an adult high-angle case

Xue-Dong Wang,Jie-Ni Zhang,Da-Wei Liu,Fei-fei Lei,Yan-Heng Zhou 대한치과교정학회 2016 대한치과교정학회지 Vol.46 No.4

In the present report, we describe the successful use of miniscrews to achieve vertical control in combination with the conventional sliding MBT<SUP>TM</SUP> straightwire technique for the treatment of a 26-year-old Chinese woman with a very high mandibular plane angle, deep overbite, retrognathic mandible with backward rotation, prognathic maxilla, and gummy smile. The patient exhibited skeletal Class II malocclusion. Orthodontic miniscrews were placed in the maxillary anterior and posterior segments to provide rigid anchorage and vertical control through intrusion of the incisors and molars. Intrusion and torque control of the maxillary incisors relieved the deep overbite and corrected the gummy smile, while intrusion of the maxillary molars aided in counterclockwise rotation of the mandibular plane, which consequently resulted in an improved facial profile. After 3.5 years of retention, we observed a stable, well-aligned dentition with ideal intercuspation and more harmonious facial contours. Thus, we were able to achieve a satisfactory occlusion, a significantly improved facial profile, and an attractive smile for this patient. The findings from this case suggest that nonsurgical correction using miniscrew anchorage is an effective approach for camouflage treatment of high-angle cases with skeletal Class II malocclusion.

Mortise and Tenon-Chinese Zodiac

Xue Fei Zou 한국콘텐츠학회 2021 ICCC International Digital Design Invitation Exhib Vol.2021 No.8

A Study on Green Packaging Barriers Related to China’s Export

Xue-fei Du,Hyun-Sook Cho 한국무역통상학회 2021 무역통상학회지 Vol.21 No.1

Green packaging (GP), also known as sustainable packaging, which is considered to protect environment and save resources, was meeting the requirements of sustainable development. In trade area, through the important role in the ecological environment and human health, GP and its system might effect on China’s export a new trade protection measure. Here, this paper explained the definition and reimbursement of GP/GPB. It also provided packaging regulations in China’s major trading partners such as the European Union, the United States and South Korea and showed some trade cases related to packaging. Finally, in order to deal with GPBs effectively and eliminate or be weaken the impact of GPBs on China’s export trade, this paper proposed some suggestions. It is hoped that through the study of this paper, the research and development (R&D) of GP can be promoted, and the application value of GP can be exerted in China’s foreign trade and social production.

Packaging Framework System Under International Trade Rules

Xue-Fei Du,조현숙 강원대학교 경영경제연구소 2022 Asia-Pacific Journal of Business Vol.13 No.3

Purpose - The purpose of this study was to expound the packaging framework system(PFS) under international trade rules. Design/methodology/approach - This study analyzed packaging framework system and packaging regulations and technical standards in international regulations and major countries and classified these in some standards Findings - First, the study provides packaging provisions associated with packaging regulations and technical standards (PRTS). Then, the basis of PFS in international trade rules was proposed; it is believed that the PFS was composed of packaging legal system in WTO institutional framework (WTO-PLS), international organization packaging standard system (IO-PSS) and major countries or regions packaging regulatory system (MCR-PRS). Secondly, this paper expounded relations and legal characteristics of the three packaging systems. Finally, This study investigated PRTS coverage under technical barriers to trade (TBT) and Sanitary and Phytosanitary measures (SPS). Research implications or Originality The regulations associated with PRTS have played a key role in challenges to merchandise trade in international trade. This study has significance in classifying packaging related reticulations into several criteria unlike previous studies. Therefore it is hope that this study can provide a supplementary document for the existing research of PRTS.

Association between Higher Blood Pressure and Risk of Diabetes Mellitus in Middle-Aged and Elderly Chinese Adults

Xue Yang,Jian Chen,An Pan,Jason H.Y. Wu,Fei Zhao,Yue Xie,Yi Wang,Yi Ye,Xiong-Fei Pan,Chun-Xia Yang 대한당뇨병학회 2020 Diabetes and Metabolism Journal Vol.44 No.3

Background: To examine the prospective association between higher blood pressure (BP) and risk of type 2 diabetes mellitus (T2DM) in middle-aged and elderly Chinese adults. Methods: A total of 9,642 middle-aged and elderly Chinese adults (≥45 years old; 47.30% men) without diabetes from the China Health and Retirement Longitudinal Study were included for analyses. Participants were categorized into three groups: normal BP, prehypertension, and hypertension, according to the 2010 Chinese Guidelines for the Management of Hypertension. The incidence of T2DM was determined by self-reported physician diagnosis during two follow-up surveys conducted in 2013 to 2014 and 2015 to 2016. Results: During the 4-year follow-up, 429 participants (4.45%) developed T2DM, including 3.51% of the men and 5.29% of the women. The incidence rates of T2DM were 2.57%, 3.75%, and 6.71% in the normal BP, prehypertension, and hypertension groups, respectively. After adjustment for age, sex, education level, residence, smoking status, alcohol consumption, body mass index, waist circumference, and dyslipidemia, both prehypertension (odds ratio [OR], 1.32; 95% confidence interval [CI], 0.98 to 1.77) and hypertension (OR, 2.02; 95% CI, 1.54 to 2.64) were associated with increased risk of T2DM, compared to those with a normal BP. The ORs associated with T2DM were 1.08 (95% CI, 1.03 to 1.13) for an increase of 10 mm Hg in systolic BP and 1.06 (95% CI, 1.01 to 1.10) for an increase of 5 mm Hg in diastolic BP. Conclusion: Higher BP is a risk factor for T2DM in middle-aged and elderly Chines. It may be a potential target for diabetes prevention.

High Efficiency Onboard Charger Based on Two-Stage Circuit

Xue Fei,Ma Xin,Li Hongqiang,Zhang Di,Xu Hengshan,Zhou Lei,Wang Chao 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.4

On-board charger (OBC) is key part of electric vehicles. Limited to space and weight, design objectives of OBC are high power-density and high effi ciency. Two-stage circuit is commonly used for 3.3 kW OBC, interleaved power factor correction (ILPFC) is utilized for power factor correction and DC bus voltage regulation, LLC resonant converter is utilized for voltage and power regulations. In this paper, the relationships between the internal parameters and effi ciency of ILPFC are studied by discrete iterative method, and the internal parameters are optimized to improve ILPFC`s effi ciency. Meanwhile, the relationships between the resonant parameters and effi ciency of LLC converter are also studied by fundamental harmonic approximation method to optimize the effi ciency in wide charging voltage. A 3.3 kW OBC prototype is developed to verify the eff ectiveness and correctness of the optimal method, the power factor and total harmonic distortion at full-load state are about 99.99% and 2.98% with the charging voltage ranging from 230 to 430 V, respectively

A Color-Reaction-Based Biochip Detection Assay for RIF and INH Resistance of Clinical Mycobacterial Specimens

( Wen Fei Xue ),( Jing Fu Peng ),( Xiao Li Yu ),( Shu Lin Zhang ),( Boping Zhou ),( Dan Qing Jiang ),( Jian Bo Chen ),( Bing Bing Ding ),( Bin Zhu ),( Yao Li ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1

The widespread occurrence of drug-resistant Mycobacterium tuberculosis places importance on the detection of TB (tuberculosis) drug susceptibility. Conventional drug susceptibility testing (DST) is a lengthy process. We developed a rapid enzymatic color-reaction-based biochip assay. The process included asymmetric multiplex PCR/templex PCR, biochip hybridization, and an enzymatic color reaction, with specific software for data operating. Templex PCR (tem- PCR) was applied to avoid interference between different primers in conventional multiplex- PCR. We applied this assay to 276 clinical specimens (including 27 sputum, 4 alveolar lavage fluid, 2 pleural effusion, and 243 culture isolate specimens; 40 of the 276 were non-tuberculosis mycobacteria specimens and 236 were M. tuberculosis specimens). The testing process took 4.5 h. A sensitivity of 50 copies per PCR was achieved, while the sensitivity was 500 copies per PCR when tem-PCR was used. Allele sequences could be detected in mixed samples at aproportion of 10%. Detection results showed a concordance rate of 97.46% (230/236) in rifampicin resistance detection (sensitivity 95.40%, specificity 98.66%) and 96.19% (227/236) in isoniazid (sensitivity 93.59%, specificity 97.47%) detection with those of DST assay. Concordance rates of testing results for sputum, alveolar lavage fluid, and pleural effusion specimens were 100%. The assay provides a potential choice for TB diagnosis and treatment.