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Xue-Chang Wu,Wei-Feng Chen,Chao-Dong Qian,Ou Li,Ping Li,Yan-Ping Wen 한국미생물학회 2007 The journal of microbiology Vol.45 No.6
A new antagonistic strain of actinomycete, designated AP19-2, was isolated from the feces of giant pandas inhabiting the Foping National Nature Reserve in China. Cultural characteristic studies strongly suggested that this strain is a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene of strain AP19-2 evidenced profound similarity (97-99%) with other Streptomyces strains. Two pure active molecules were isolated from a fermentation broth of Streptomyces sp. strain AP19-2 via extraction, concentration, silica gel G column chromatography, and HPLC. The chemical structures of the two related compounds (referred to as chromomycin A2 and chromomycin A3) were established on the basis of their Infrared spectra (IR), High Resolution Electrospray Ionization Mass Spectrometry (HR-ESI-MS), and Nuclear Magnetic Resonance (NMR) data, and by comparison with published data.
Xue Wu,Zhidong Chang,Jingge Feng,Hui Dang,Hasan Uslu,Wenjun Li 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.66 No.-
In order to get optimal extraction performance as well as ultra-low solvent residual, the extraction behavior of tris(2,2,3,3,4,4,4-heptafluorobutyl) phosphate (FTBP) dissolved in 2,3,4,5,6-Pentafluorotoluene (FB) was evaluated to investigate the influence of the aggregation state on the extraction of salicylic acid (SA). The effect of SA distribution and water content on aggregation structure in organic phase was assessed through analysis of SAXS data. Based on molecular dynamic simulation, FTBP tended to aggregate with the existence of FB to accommdate more SA molcules. Water in this case was not the critical factor for aggregation formation due to the hydrophobicity of fluorinated solvents.
Xue Wu,Zhidong Chang,Junchao Yuan,Jingge Feng,Hasan Uslu,Wenjun Li 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.58 No.-
In order to get optimal extraction performance as well as ultra-low solvent residual, three fluorinated hexane, perfluorohexane (C6F14), 1H-perfluorohexane (C6F13H) and 1H,6H-perfluorohexane (C6F12H2), were evaluated as diluents for synergistic effect on extraction capability of 1H,1H,2H,2H-Perfluorooctan-1-ol by the way of experimental and computational investigation. Based on extraction experiments, C6F14 showed obvious antagonistic effect because of hydrophobic nature, while the synergistic effect of C6F12H2 was better than that of C6F13H. According to DFT calculation, supported by FT-IR and 1H NMR spectra, the synergistic effect was illustrated as that fluorinated hexane could interact with salicylic acid-fluorinated octanol complex through hydrogen bond.
Legumain Protein as a Potential Predictive Biomarker for Asian Patients with Breast Carcinoma
Wu, Mei,Shao, Guang-Rui,Zhang, Fei-Xue,Wu, Wen-Xiu,Xu, Ping,Ruan, Zheng-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24
Background: Treatment for breast cancer is mainly performed by surgical resection of primary tumors and chemotherapy. However, after tumor invasion and metastases, breast cancer is hard to control. Clarification of the pathogenic mechanisms would be helpful to the prognosis or therapy for the breast cancer. The aim of this study is to investigate the clinical and prognostic implications of legumain protein Materials and Methods: In this study, we examined mastectomy specimens from 114 breast cancer and matching, 26 adjacent non-cancerous tissues using immunohistochemistry. Results: The results indicated that positive expression of legumain protein in breast cancer was 51.8 % (59/114) and the positive expression of legumain protein in adjacent non-cancerous tissue was 11.5% (3/26). It appeared to be related with lymph node metastasis of breast cancer (p=0.02) and correlation analysis indicated that legumain expression was correlated positively with the estrogen receptor (ER) and mutant-type p53 expression (both p<0.05). Positive legumain expression was significantly associated with shorter overall survival time in breast cancer patients (log-rank p<0.01). Multivariate survival analysis suggested that the positive legumain expression was an independent predictor of poorer overall survival in patients with breast cancer (HR=0.24; 95%CI 0.11-0.65, p=0.03). Conclusions: Legumain might be a new potential biomarker for breast cancer, which may reflect the prognosis and overall survival.
( Xue Zhuang Wu ),( Xiu Hua Gao ),( Fu He Yang ) 한국동물자원과학회(구 한국축산학회) 2015 한국축산학회지 Vol.57 No.6
The objectives of this study were to study the effects of different levels of dietary copper on organ indexes, tissular Cu, Zn and Fe deposition and fur quality of mink in the growing-furring periods. One hundred and five standard dark male mink were randomly assigned to seven groups with the following dietary treatments: basal diet with no supplemental Cu (Control); basal diet supplemented with either 6, 12, 24, 48, 96 and 192 mg/kg Cu from copper sulphate, respectively. The colour intensity scores displayed a linear trend (P = 0.057). The spleen Cu concentrations responded in a linear (P < 0.05) fashion with increasing level of Cu, but copper supplementation did not affect speen concentrations of Fe or Zn. Supplemental dose of Cu linearly increased (P < 0.05) liver Cu and Fe concentrations but did not alter (P > 0.10) liver Zn. Our results indicate that Cu plays an important role in the pigmentation in growing-furring mink, and supplemental dietary Cu in growing-furring mink improve hair colour, and copper has limited effects on liver mineral deposition.
Wu, Xue-Chang,Chen, Wei-Feng,Qian, Chao-Dong,Li, Ou,Li, Ping,Wen, Yan-Ping The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.6
A new antagonistic strain of actinomycete, designated AP19-2, was isolated from the feces of giant pandas inhabiting the Foping National Nature Reserve in China. Cultural characteristic studies strongly suggested that this strain is a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene of strain AP19-2 evidenced profound similarity (97-99 %) with other Streptomyces strains. Two pure active molecules were isolated from a fermentation broth of Streptomyces sp. strain AP19-2 via extraction, concentration, silica gel G column chromatography, and HPLC. The chemical structures of the two related compounds (referred to as chromomycin $A_2$ and chromomycin $A_3$) were established on the basis of their Infrared spectra (IR), High Resolution Electrospray Ionization Mass Spectrometry (HR-ESI-MS), and Nuclear Magnetic Resonance (NMR) data, and by comparison with published data.
Wu, Guo-Qiu,Liu, Nan-Nan,Xue, Xiu-Lei,Cai, Li-Ting,Zhang, Chen,Qu, Qing-Rong,Yan, Xue-Jiao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10
Background: This study was aimed to establish a novel method to simultaneously detect expression of four genes, ribonucleotide reductase subunit M1(RRM1), X-ray repair cross-complementing gene 1 (XRCC1), thymidylate synthase (TS) and class III ${\beta}$-tubulin (TUBB3), and to assess their application in the clinic for prediction of response of non-small cell lung cancer (NSCLC) to chemoradiotherapy. Materials and Methods: We have designed four gene molecular beacon (MB) probes for multiplex quantitative real-time polymerase chain reactions to examine RRM1, XRCC1, TUBB3 and TS mRNA expression in paraffin-embedded specimens from 50 patients with advanced or metastatic carcinomas. Twenty one NSCLC patients receiving cisplatin-based first-line treatment were analyzed. Results: These molecular beacon probes could specially bind to their target genes in homogeneous solutions. Patients with low RRM1 and XRCC1 mRNA levels were found to have apparently higher response rates to chemoradiotherapy compared with those with high levels of RRM1 and XRCC1 expression (p<0.05). The TS gene expression level was not significantly associated with chemotherapy response (p>0.05). Conclusions: A method of simultaneously detecting four molecular markers was successfully established and applied for evaluation of chemoradiotherapy response. It may be a useful tool in personalized cancer therapy.
Black Hole Mass and Accretion Rate of Active Galactic Nuclei
Xue-Bing Wu,F.K. Liu,J.L. Han,M.Z. Kong,R. Wang 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.4
The determination of the central black hole mass is crucial to the understanding of active galactic nuclei physics. In this paper, we brie°y review some methods that are currently used to estimate the black hole mass of AGNs. Particularly, we demonstrate the importance of two correlations: one between the black hole mass and the stellar velocity dispersion and the other one between the broad line region (BLR) size and the optical continuum luminosity. Besides applying these relations in deriving the black hole masses of various types of AGNs, we also employ the fundamental plane of elliptical galaxies to estimate the central velocity dispersions of AGN host galaxies, and then the black hole masses of AGNs, including BL Lac objects. In addition, we derive another empirical relation between the BLR size and the H. emission line luminosity from AGNs with the BLR size measured by reverberation mapping studies, and we argue that more accurate black hole masses of extremely radio-loud AGNs can be obtained with it than by using the usual R ¡ L 5100ºA relation because of the jet contribution to the optical continuum. Finally, we point out that black hole mass estimation is very much helpful in determining the accretion rate and in understanding the accretion process in AGNs.
THE INSTABILITIES OF ACCRETION DISKS WITH RADIAL ADVECTION
WU XUE-BING The Korean Astronomical Society 1996 Journal of The Korean Astronomical Society Vol.29 No.suppl1
The local instabilities of accretion disks were extensively studied, with the considerations of radial advection, thermal diffusion and different disk geometry, dominated pressure and optical depth. Two inertial-acoustic modes in a geometrically thin, radiative cooling dominated disk depart from each other if very little advection is included. A geometrically slim, advection-dominated disk is found to be always stable if it is optically thin. However, if it is optically thick, the thermal diffusion has no effect on the stable viscous mode but has a significant contribution to enhance the thermal instability.