바터팽대부 선암의 β-catenin 표현

현연화,구지혜,이강,박선미,성노현 충북대학교 의과대학 충북대학교 의학연구소 2010 忠北醫大學術誌 Vol.20 No.1

Purpose : Carcinomas of the ampulla of Vater have either intestinal or pancreatobiliary type differentiation. The molecular pathogenesis of both histologic types of ampullary carcinoma are not yet well characterized. The role of β-catenin pathway is important in colorectal carcinogenesis and β-catenin protein expression is dysregulated in the early stage of the adenoma-carcinoma sequence. The aim of this study is to investigate the differences between dysregulated β-catenin expression in the carcinogenesis of two histologic types of carcinoma of the ampulla of Vater. Materials and Methods : We examined 24 cases of resected carcinoma of the ampulla of Vater. Serial sections of all carcinoma specimens were stained with antibodies to cytokeratins (CK7, CK20) and β-catenin. Normal expression of β-catenin was defined as exclusive membranous staining. Dysregulated expression of β-catenin was defined as cytoplasmic staining in more than 50% of tumor cells and/or nuclear staining. Results : Ten carcinomas (42%) were immunohistochemically intestinal type (CK7-, CK20+) and eleven carcinomas (46%) were pancreatobiliary type (CK+, CK20-). Three carcinomas were immunohistochmically other types (CK7+, CK20+ or CK7-, CK20-). Abnormal β-catenin was accumulated in nine cases (90%) of intestinal type. Among them, nuclear staining was noted in two cases with moderately and poorly differentiated histology respectively. In pancreatobiliary type, only one case (9%) with poorly differentiated histology showed abnormal β-catenin expression in tumor nuclei. Conclusion : Dysregulation of β-catenin expression may play critical role in the carcinogenesis of intestinal type, but not of pancreatobiliary type. Abnormal accumulation of β-catenin in tumor nuclei may be related to poorly differentiated histology in both types of ampullary carcinoma. 연구 목적: 바터팽대부에서 발생하는 선암의 대부분은 조직학적으로 장형과 췌담형으로 구성된다. 두 병변의 발암기전은 뚜렷하게 밝혀진 바는 없으나, 췌담형 선암은 췌담도 선암의 발암기전과 유사하고, 장형 선암은 장 선암의 발암기전과 유사하다고 추측한다. 대부분의 장 선암에서는 암 발생 초기에 세포 안에 β-catenin의 축적이 발생하며, 축적된 β-catenin이 전암 세포 또는 암 세포의 증식을 유발하는 중요한 역할을 한다. 그러나 대부분의 췌담도 선암의 발생 과정에서는 β-catenin 축적에 의한 세포 증식은 중요한 역할을 하지 않는다. 바터팽대부에서 발생하는 장형 선암과 췌담형 선암의 발암 과정의 유사점 또는 차이점을 규명하기 위하여, 장 선암의 발암 과정에서는 중요한 역할을 하지만 췌담도의 선암의 발암 과정에는 역할이 중요하지 않은 β-catenin의 축적으 면역조직화학 방법으로 비교하였다. 재료 및 방법: 충북대학교병원에서 외과적 수술로 절제한 바터팽대부에서 발생한 선암 24 예의 조직 slide를 현미경으로 관찰하고, 각 증례의 병변을 대표하는 조직의 paraffin 포매 block 한 개를 선택하여 박편을 제작하였다. 장형 선암과 췌담형 선암을 면역조직화학 방법으로 구분하기 위하여 각 박편에 통상적인 avidin-biotin immunoperoxidase 방법으로 cytokeratin 7과 cytokeratin 20 면역 염색을 하였으며, 발암기전의 차리를 알기 위하여 β-catenin 면역 염색을 하였다. β-catenin의 축적은 종양 세포의 50% 이상에서 세포질에 뚜렷하게 염색된 경우와 종양 세포의 핵에 염색된 경우를 양성으로 하였다. 결과: 10 예는 cytokeratin 7에 음성. cytokeratin 20에 양성이어서 장형 선암으로 분류하였다. 11 예는 cytokeratin 7에 양성. cytokeratin 20에 음성이어서 췌담형 선암으로 분류하였다. 3 예는 cytokeratin 7과 cytokeratin 20에 모두 양성 또는 모두 음성이엇 연구에서 제외하였다. 장형 선암은 9 예(90%)에서 β-catenin의 축적이 나타났다. 종양 세포 핵의 β-catenin 축적은 중등도 분화 암 3 예 중에서 1 예. 저등도 분화 암 1 예 중 1 예에서 관찰되었다. 고등도 분화 암에 동반된 선종 2 예에서도 종양 세포의 세포질에 β-catenin의 축적이 관찰되었다. 췌담형 선암은 11 예 중에서 1 예(9%)에서 β-catenin의 축적이 나타났으며 나머지 10 예는 세포막에만 염색되었다. β-catenin의 축적은 저등도 분화 암 1 예 중에서 1 예에 나타났으며 세포 핵에서 β-catenin의 축적이 나타났다. 결론: 바터팽대부에서 발생하는 장형 선종과 선암의 발암 과정에는 β-catenin의 축적이 중요한 역할을 하며, 장형 선암의 발암 과정은 장의 선종-선암 과정과 유사함을 시사하였다. 바터팽대부에서 발생하는 대부분의 췌담형 선암 세포에서는 β-catenin의 축적이 발생하지 않았으므로 췌담형 선암의 발암기전은 장형 선암의 발암기전과 다름을 시사하였다. 그러나 β-catenin의 종양 세포 핵 축적은 바터팽대부에서 발생하는 장형과 췌담형 선암의 악성화와 관계가 있었다.

VULCANIZATION CONTROLLER

Ji, Xuan Yong 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1

Vulcanization is the last process of rubber production. It is directly related to quality. In order to control the process of rubber vulcanization scientifically, I devise a vulcanization controller with 80 31 single-chip computer. This paper briefly introduce principal characteristics and control ideas, applications of the controller.

VULCANIZATION CONTROLLER

Xuan Yong Ji 대한전자공학회 1992 대한전자공학회 Conference Vol.1992 No.2

A Novel Clotrimazole-Ioaded Suppository with Effective Anti-tumor Activity

Xuan, Jing Ji,Kim, Jong-Tae,Oh, Dong-Hoon,Han, Hong-Hee,Lee, Won-Seok,Lee, Jong-Sook,Rhee, Jong-Dal,Yong, Chul-Soon,Woo, Jong-Soo,Kim, Jung-Ae,Choi, Han-Gon The Korean Society of Pharmaceutical Sciences and 2008 Journal of Pharmaceutical Investigation Vol.38 No.5

To develop a poloxamer-based solid suppository with poloxamer and polyethylene glycol mixtures, the melting point of various formulations composed of P 188 and propylene glycol were investigated. The dissolution and antitumor activity of clotrimazole delivered by the poloxamer-based suppository were performed. The poloxamer mixtures composed of P 188 and propylene glycol were homogeneous phases. P 188 greatly affected the melting point of poloxamer mixtures. In particular, the poloxamer mixture [P 188/propylene glycol(70/30%)] with the melting point of about $32^{\circ}C$ was a solid form at room temperature and instantly melted at physiological temperature. Furthermore, the ratio of P 188/propylene glycol greatly affected the dissolution rates of clotrimazole from poloxamer-based suppository. It gave the more effective anti-tumor activity than conventional PEG-based suppository due to fast dissolution. Thus, the clotrimazole-Ioaded poloxamer-based solid suppository was an effective rectal dosage form with anti-tumor activity.

중국 지역경제 동조현상 원인 분석

( Chun Ji Xuan ),이기영 ( Ki Ryoung Lee ) 한중사회과학학회 2014 한중사회과학연구 Vol.12 No.3

This thesis has analyzed what causes have brought in synchronous phenomena among regional economies in that there has been difference among regional economies amidst the economic movements(fluctuations) due to differences of industrial structures, marketisation, governmental interference, open foreign trade, and economic levels. This study was carried out by factor analysis, and principal composition analysis, whose results show that 30 regional economies from factor 1 to factor 7 reveal high inter-relationship either singular or plural, proving to synchronize with these factors. The outcome of analysis via characteristics of the regions which synchronized with the factors show that factor 1 represents characteristics of the passive regions in their relation to the central government or its policies. Factor 2 those of the regions where agriculture is developed, and factor 3 those of China’s major economic growth hubs since the reform and opendoor policy represented by 北京, 上海, 廣東, while factor 4 those of the regions where energy/mining industry is developed such as petroleum, natural gas, and iron ore etc. Factor 5 represents those of logistics-centric regions with seaports while factor 6 those of the southwest economic sphere where economy is relatively retarded. Factor 7 represents those of industrially developed regions. though they move to their regional characteristics, also fluctuate to other factors. As assumed by hypothesis of this study, it has been confirmed that regions, though geographically apart from one other, show synchronous phenomena of economic fluctuations depending upon their industrial characteristics, policies of the central government, and their relationship with the central government.

Development of thermo-sensitive injectable hydrogel with sustained release of doxorubicin: Rheological characterization and in vivo evaluation in rats

( Jing Ji Xuan ),( Yi Dong Yan ),( Dong Hoon Oh ),( Young Keun Choi ),( Chul Soon Yong ),( Han Gon Choi ) 영남대학교 약품개발연구소 2011 영남대학교 약품개발연구소 연구업적집 Vol.21 No.-

A Novel Clotrimazole-Loaded Suppository with Effective Anti-tumor Activity

( Jing Ji Xuan ),( Jong Tae Kim ),( Dong Hoon Oh ),( Hong Hee Han ),( Won Seok Lee ),( Jong Sook Lee ),( Jong Dal Rhee ),( Chul Soon Yong ),( Jong Soo Woo ),( Jung Ae Kim ),( Han Gon Choi ) 한국약제학회 2008 Journal of Pharmaceutical Investigation Vol.38 No.5

Classification of Korean Rice Cultivars based on Reaction Pattern to Japanese Isolates of Blast Pathogen

Jin, Xuan-Ji,Lee, Eun-Jeong,Choi, Jae-Eul The Korean Society of Crop Science 2007 Journal of crop science and biotechnology Vol.10 No.1

Classification of blast resistance type of 129 Korean rice cultivars was carried out based on reaction pattern to 10 Japanese blast pathogen isolates(Pyricularia oryzae). The cultivars were divided into 11 groups based on the presumed resistance genes as follows; Pia type(19 cultivars), Pita-2 type(4), Pik type(3), Pib type(5), Piz type(11), Pik-s type(8), Pik and Pii type(4), Pia and Pita type(8), Pia and Pik type(6), Pita, Pik and Pii type(4) and no-grouping type(57). These results would provide important information to rice breeding for durable and broad resistance to rice blast.

Nicotine exacerbates tacrolimus-induced renal injury by programmed cell death

( Yu Ji Jiang ),( Sheng Cui ),( Kang Luo ),( Jun Ding ),( Qi Yan Nan ),( Shang Guo Piao ),( Mei Ying Xuan ),( Hai Lan Zheng ),( Yong Jie Jin ),( Ji Zhe Jin ),( Jung Pyo Lee ),( Byung Ha Chung ),( Bum 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.6

Background/Aims: Cigarette smoking is an important modifiable risk factor in kidney disease progression. However, the underlying mechanisms for this are lacking. This study aimed to assess whether nicotine (NIC), a major toxic component of cigarette smoking, would exacerbates tacrolimus (TAC)-induced renal in-jury. Methods: Sprague-Dawley rats were treated daily with NIC, TAC, or both drugs for 4 weeks. The influence of NIC on TAC-caused renal injury was examined via renal function, histopathology, oxidative stress, mitochondria, endoplasmic reticulum (ER) stress, and programmed cell death (apoptosis and autophagy). Results: Both NIC and TAC significantly impaired renal function and histopathology, while combined NIC and TAC treatment aggravated these parameters beyond the effects of either alone. Increased oxidative stress, ER stress, mitochondrial dysfunction, proinflammatory and profibrotic cytokine expressions, and programmed cell death from either NIC or TAC were also aggravated by the two combined. Conclusions: Our observations suggest that NIC exacerbates chronic TAC nephrotoxicity, implying that smoking cessation may be beneficial for transplant smokers taking TAC.

메조공극 산화철를 이용한 수중 5가비소 제거

배지열 ( Ji Yeol Bae ),박호식 ( Ho Sik Park ),김요한 ( Yo Han Kim ),( Tung Xuan Bui ),박지해 ( Ji Hae Park ),최희철 ( Hee Chul Choi ) 한국물환경학회 2010 공동 추계학술발표회 Vol.2010 No.-