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( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.
Xu, Guo-Bo,Yang, Tao,Bao, Jin-Ku,Fang, Dong-Mei,Li, Guo-You 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5
Isochaetomium $A_2$ (1), a new bis(naphthodihydropyran-4-one), along with chaetochromins A (2) and B (3), was isolated from the solid-state fermented rice culture of Chaetomium microcephalum. The structure of compound 1 was elucidated on the basis of 1D and 2D NMR spectral data, and the relative configuration was confirmed by CD spectrum. Compounds 1-3 possessed significant antimicrobial activity against Escherichia coli 1.044, Staphylococcus aureus 1.252, and Bacillus subtilis 1.079. Moreover, compounds 1-3 showed obvious inhibitory effects on mouse spleen cell proliferation with successive $IC_{50}$ values of 0.52, 0.19, and $0.24{\mu}M$.
Xu, Tao,Yuan, Rui,Xu, Peng-Cheng,Pan, Dong-Jie,Yang, Woochul,Guo, Hai-Bo,Shen, Yu-Fang,Hu, Jian-Feng,Zhang, Zhi-Jun,Zhao, Jing-Tai Elsevier 2018 Journal of alloys and compounds Vol.762 No.-
<P><B>Abstract</B></P> <P>Uniform, monodisperse yttrium aluminum garnet (YAG) rhombic dodecahedron micro-crystals were synthesized using coprecipitation and hydrothermal methods. The Na<SUB>2</SUB>SO<SUB>4</SUB> as a surfactant was helpful in improving the dispersity of the crystallites and forming well-faceted, micro-sized dodecahedral YAG crystallites. The effect of the reaction time and the solvent has also been studied. The crystal growth mechanism called dissolution/crystallization was determined by analyzing the experimental and simulated results. And the specific well-developed crystal facets in the {110} family have been demonstrated by the theoretical calculation. A higher integrated emission intensity can be achieved for the YAG:Ce crystallites by improving the phase purity, morphology with better developed dodecahedrons, and dispersion. Furthermore, a rather high luminous efficacy (LE = 104.14 lm/W) for the white LED fabricated using the YAG:Ce<SUP>3+</SUP> phosphor prepared in this work was realized. The correlation between the crystal growth, morphology and luminescence properties of YAG crystallites has been established, which exhibits a great importance for the synthesis and application of mono-dispersed YAG crystallites.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Uniform and monodisperse YAG rhombic dodecahedron micro-crystals were synthesized. </LI> <LI> The growth mechanism of YAG rhombic dodecahedron was studied through experimental and computational simulation. </LI> <LI> The correlation between the crystal growth, morphology and luminescence of YAG crystallites was established. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Tribological performance of surface treated piston assembly with infiltrated layer
Bo Xu,Bifeng Yin,Dashu Gao,Xijun Hua 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1
By applying surface treatment on piston assembly and typical samples, an infiltrated layer with the depth of about 30 μm was formed on the treated sample surface. The surface hardness and linear expansion coefficient of treated piston sample decreased, while the hardness of treated ring sample increased. The results of ball-on-disc rotating friction test indicated that the infiltrated layer is helpful for improving the tribological properties of aluminum alloy, reducing friction coefficient under both dry friction and oil lubricated conditions, and improving the anti-friction and wear resistance performance of cast iron. In addition, the reciprocating friction testing results showed that surface treatment technology has stable antifriction effect on piston/liner pair under various working conditions, with the average friction coefficient being reduced by about 10.2-22.1 %; while the anti-friction effect on ring/liner pair is mainly reflected under low-speed heavy-load condition, with the average friction coefficient being reduced by approximately 7.2-9.9 %.
Xu, Heng,Tian, Yan-Na,Dun, Bo-Ying,Liu, Hai-Tao,Dong, Guang-Kuo,Wang, Jin-Hua,Lu, Shang-Su,Chen, Bo,She, Jin-Xiong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11
A novel monoclonal antibody (mAb), known as AC10364, was identified from an antibody library generated by immunization of mice with human carcinoma cells. The mAb recognized proteins in lysates from multiple carcinoma cell lines. Cell cytotoxicity assays showed that AC10364 significantly inhibited cell growth and induced apoptosis in multiple carcinoma cell lines, including Bel/fu, KATO-III and A2780. Compared with mAb AC10364 or chemotherapeutic drugs alone, the combination of mAb AC10364 with chemotherapeutic drugs demonstrated enhanced growth inhibitory effects on carcinoma cells. These results suggest that mAb AC10364 is a promising candidate for cancer therapy.
OFPT: OpenFlow based Parallel Transport in Datacenters
( Bo Liu ),( Bo Xu ),( Chao Hu ),( Hui Hu ),( Ming Chen ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.10
Although the dense interconnection datacenter networks (DCNs) (e.g. FatTree) provide multiple paths and high bisection bandwidth for each server pair, the single-path TCP (SPT) and ECMP which are widely used currently neither achieve high bandwidth utilization nor have good load balancing. Due to only one available transmission path, SPT cannot make full use of all available bandwidth, while ECMP`s random hashing results in many collisions. In this paper, we present OFPT, an OpenFlow based Parallel Transport framework, which integrates precise routing and scheduling for better load balancing and higher network throughput. By adopting OpenFlow based centralized control mechanism, OFPT computes the optimal path and bandwidth provision for each flow according to the global network view. To guarantee high throughput, OFPT dynamically schedules flows with Seamless Flow Migration Mechanism (SFMM), which can avoid packet loss in flow rerouting. Finally, we test OFPT on Mininet and implement it in a real testbed. The experimental results show that the average network throughput in OFPT is up to 97.5% of bisection bandwidth, which is higher than ECMP by 36%. Besides, OFPT decreases the average flow completion time (AFCT) and achieves better scalability.
SIMULATION OF MIXED GAS FORMATION FOR A SPRAY-WALL COMPLEX GUIDED LPG DIRECT INJECTION ENGINE
Bo-yan Xu,Xiang-long Liu,Long-long Jiang,Juan Xu 한국자동차공학회 2017 International journal of automotive technology Vol.18 No.3
To obtain an ultralean air-fuel ratio and to reduce engine-out NOX and HC emissions induced by the richer mixture near the spark plug, a spray and wall complex guided combustion system has been developed by utilizing the fuel characteristics of LPG. The new combustion system configuration is optimized by using a commercial CFD code, FIRE V2013, and the reliability of the system has been experimentally demonstrated by Plane Laser-Induced Fluorescence (PLIF). The mixture formation in the new combustion system under part load (2,000 rpm) is numerically simulated. With an injection timing of 40°CA BTDC, the LPG spray which is injected from two upper holes, reaches the ignition point, and the other part of the LPG spray which is injected from the bottom hole, is directed to the ignition point through the vertical vortices at the same time. At the ignition timing of about 20°CA BTDC, the two-part mixtures have been shown to form a stable and richer stratified mixture around the ignition point, and the maximum global air-fuel ratio reaches to 60 : 1.