Synergistic Inhibitory Effect of Berberine and d-Limonene on Human Gastric Carcinoma Cell Line MGC803

Xiu-Zhen Zhang,Ling Wang,Dongwu Liu,Guang-Yan Tang,Hongyu Zhang 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.9

This study aims at evaluating the anticancer effects of berberine hydrochloride (berberine) and d-limonene, alone and in combination, on human gastric carcinoma cell line MGC803 to determine whether berberine and d-limonene work synergistically and elucidate their mechanisms. MGC803 cells were treated with berberine and d-limonene, alone and in combination, for 24–48 h. The inhibitory effects of these drugs on growth were determined by MTT assay. The combination index and drug reduction index were calculated with the Chou–Talalay method based on the median-effect principle. Flow cytometry and laser scanning confocal microscopy were employed to evaluate the effects of both drugs on cell-cycle perturbation and apoptosis, generation of reactive oxygen species (ROS), mitochondrial membrane potential, and expression of Bcl-2 and caspase-3 in MGC803 cells. Berberine or d-limonene alone can inhibit the growth of MGC803 cells in a doseand time-dependent manner. Berberine and d-limonene at a combination ratio of 1:4 exhibited a synergistic effect on anti-MGC803 cells. The two drugs distinctly induced intracellular ROS generation, reduced the mitochondrial transmembrane potential (DCm), enhanced the expression of caspase-3, and decreased the expression of Bcl-2. The combination of berberine and d-limonene showed more remarkable effects compared with drugs used singly in MGC803 cells. The combination of berberine and d-limonene exerted synergistic anticancer effects on MGC803 cells by cell-cycle arrest, ROS production, and apoptosis induction through the mitochondria-mediated intrinsic pathway.

Flavor Components Comparison between the Neck Meat of Donkey, Swine, Bovine, and Sheep

Xiu Li,Issoufou Amadou,Guang-Yun Zhou,Li-Yan Qian,Jian-Ling Zhang,Dong-Liang Wang,Xiang-Rong Cheng 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.4

Donkey in China is well known for its draft purpose and transportation; however, donkey meat has attracted more and more consumers in recent years, yet it lacks sufficient information on its flavor components compared to other main meats. Therefore, in this study, volatile flavor compounds in neck meat of donkey, swine, bovine, and sheep were classified by electronic nose, then confirmed and quantified by gas chromatography-mass spectrometry. High-performance liquid chromatography (HPLC) and gas chromatography were used to quantify free fatty acid, amino acid, and flavor nucleotide. A total of 73 volatile compounds were identified, and aldehydes were identified as the characteristic flavor compounds in neck meat of donkey, bovine, swine and sheep in proportion of 76.39%, 46.62%, 31.64%, and 35.83%, respectively. Particularly, hexanal was the most abundant volatile flavor. Compared with other neck meat, much higher unsaturated free fatty acids were present in donkeys. Furthermore, neck meat of donkeys showed essential amino acid with highest content. Thus, special flavor and nutrition in donkey neck meat make it probably a candidate for consumers in other regions besides Asia.

Two Novel 3-D Zinc Compounds with Mixture Ligands of H3BTB and 4,4'-Bipy: Crystal Structures, Blue Fluorescence and Single Point Energy Calculation

Xiu-Cheng Zhang,Ling Xu,Wen-Guang Liu,Bing Liu 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.9

Two 3-D zinc framework compounds, [Zn6(BTB)4(4,4'-bipy)4(H2O)4]․9H2O (1) and [Zn3(BTB)2(4,4'-bipy)2(H2O)2]․5H2O (2) (H3BTB = 1,2,3-benzenetricarboxylic acid, 4,4'-bipy = 4,4'-bipyridine), are obtained from the diffusion method and hydrothermal reaction respectively. Though 1 and 2 has the same coordination geometries of zinc atoms and coordination mode of BTB3‒, their 2-D layers are different: mirror symmetric layers in 1; parallel ones in 2,further connecting by 4,4'-bipy into 3-D frameworks. The hydrothermal reaction of 2 results in a more stable 3-D framework than the one in 1, which is supported by the single point energy calculations. 1 and 2 show similar blue fluorescence at 417 nm, which can be assigned to LMCT.

Two d10 Metal Coordination Polymers Based on 1H-1,2,4-Triazole: Synthesis, Structure and Fluorescence

Xiu-Cheng Zhang,Ling Xu,Wen-Guang Liu,Bing Liu 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.5

The reactions of 1H-1,2,4-triazole (Htr) with MX2 (ZnCl2 for 1; CdBr2 for 2) resulted in two coordination polymers, [Zn(tr)Cl]_n (1) and [Cd(Htr)_2Br_2]_n (2). The structural analyses indicate that 1 and 2 feature a 2D layer and 1D triple chain, respectively. In 1, neighouring Zn atoms are connected by μ_3-1 κN: 2 κN: 4 κN-tr^− anionic ligand into 6- and 16-membered rings, further grow into a 2D sheet. Cd atoms in 2 are bonded by two μ_2-Br^−bridges and neutral μ_2-1 κN: 2 κN-Htr to form a 1D triple chain. The fluorescent characterizations of 1, 2 and the free Htr ligand feature simlilar emission peakes at 444, 446 and 423 nm respectively, which can be assigned to intra-ligand π-π^* transition of (H)tr. The energy gaps of 5.90 eV for 1, 5.16 eV for 2, and 5.93 eV for Htr suggest that the compounds behave as insulators.

Expression profiles and ligand-binding properties of two odorant-binding proteins from Grapholita molesta (Busck) (Lepidoptera: Tortricidae)

Guang Wei Li,Xiu Lin Chen,Yong Sun,Yu Xing Chen,Shi Cai Xu,Jun Xiang Wu 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.4

The ligand-binding function of odorant binding proteins (OBPs) that are expressed exclusively or enriched in antennae are well-studied, whilst the ligand binding properties of relatively low expressed OBPs in insect antennae are still unclear. Here, two low expressed GmolOBPs (namely GmolOBP12 and GmolOBP16) were cloned based on the antennal transcriptome of Grapholita molesta, and then their expression profiles and binding properties were investigated via real-time quantitative PCR (qRT-PCR) and fluorescence binding assays, respectively. Multiple sequence alignment and phylogenetic tree analyses displayed that both of GmolOBP12 and GmolOBP16 possessed the typical six-cysteine motifs unique to the classic OBPs subfamily and were classified as such. Although the abundance of transcripts of GmolOBP12 and GmolOBP16 were relatively low compared to that of many other OBPs in the antennal transcriptome of G. molesta, qRT-PCR results indicated that the transcript levels of GmolOBP12 and GmolOBP16 in antennae were significantly higher than those in other tissues. GmolOBP12 displayed higher expression level in male antennae than in female antennae, while the transcript level of GmolOBP16 in antennae was similar for both sexes. Both recombinant GmolOBP12 and GmolOBP16 exhibited strong binding affinities to the sex pheromone (Z)-8-dodecenyl alcohol and host-plant volatile pear ester. Besides, rGmolOBP12 showed outstanding binding affinities to (E)-2-hexenal, benzaldehyde, 1-hexanol, and (Z)-3-hexenyl acetate with K i values of 4.21, 6.81, 4.68 and 4.68 μM, respectively. rGmolOBP16 had moderate binding abilities with hexanal, decanal, 1-hexanol, methyl myristate and benzonitrile. We speculated that GmolOBP12 may have dual functions in recognition of green leaf volatiles and sex pheromone components and GmolOBP16 may participate in the detection of host-plant volatiles in chemoreception.

Value of 3-Dimensional CT Virtual Anatomy Imaging in Complex Foreign Body Retrieval from Soft Tissues

Xiu-Jun Yang,Guang-Fu Xing,Chang-Wen Shi,Wei Li 대한영상의학회 2013 Korean Journal of Radiology Vol.14 No.2

Objective: To investigate the value of 3-dimensional (3D) CT virtual anatomy imaging (VAI) in the complex foreign body (FB) retrieval of the soft tissues. Materials and Methods: Four hundred and seventy-five patients with radiopaque FB(s) diagnosed by radiograph underwent contrast-enhanced 3D CT examination. VAI was reconstructed by volume-rendering opacity software, by sliding down the lowest threshold from -600 to 100 HU. The imaging was grouped into three groups: A (axial and multi-planar reformation [MPR] images), B (standard 3D imaging with axial and MPR images), and C (VAI with axial and MPR images). They were analyzed to reveal the type, size, number, location, complications, and the interventional removability of the object, with the comparisons in the management and clinical outcomes on the patient follow-up studies. The data were subjected to chi-square tests, with p value < 0.05 indicating significant statistical difference. Results: The FB shape, size, number, site distribution and vessels around FB, as well as the FB-associated vascular complications and the FB interventional removability were assessed more accurately in Group C than in Group B or Group A (p < 0.005). There was no significant difference in disclosing the type and depth of the FB among the three groups (p > 0.75). On the basis of the 3D CT, especially the enhanced 3D CT VAI, the followings were processed: the recommendation of interventional removal in 286 (60.47%) and non-intervention in 187 (39.53%) of the 473 patients with soft-tissue FB(s); in 352 (56.50%) of the 623 radiopaque FBs patients, 258 (54.55%) patients accurately detected on 3D CT and the successful removal by intervention (343 FBs) or surgery (9 FBs) without any sequela; and 215 (45.45%) patients with 271 FBs lost in the follow-up, with their departure from the hospital. Conclusion: The 3D CT, especially 3D enhanced CT VAI, has great incremental value in further diagnosis and management of complex FB extraction from soft tissues.

The potential molecular effects of bursal septpeptide II on immune induction and antitumor activity

Guang Fang Zhou,Qing Tao Liu,Bin Zhou,Ya Feng Qiu,Xiao Dong Liu,Zhi Yong Ma,Xiu Li Feng,Rui Bing Cao,Pu Yan Chen 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

The bursa of Fabricius (BF) is the acknowledged central humoral immune organ in birds. Bursal septpeptide II (BSP-II) is animmunomodulatory bioactive peptide isolated from BF. To understand the effects of BSP-II on immune induction, gene expression profilesof hybridoma cells treated with BSP-II were evaluated. Pathway analysis showed that regulated genes were involved in cytokine-cytokinereceptor interactions, T cell receptor signaling pathway, and pathway in cancer. It was observed that BSP-II reduced tumor cells proliferationand stimulated p53 expression. These results indicate potential mechanisms underlying the effects of the humoral immune system on immuneinduction, including antitumor activities. Our study has provided a novel insight into immunotherapeutic strategies for treating human tumors.

Hydrothermal Synthesis, Structure and Thermal Property of a New 3D Zinc Framework Containing 1,3,5-triazine-2,4,6-tricarboxylate

Xiu-Cheng Zhang,Ling Xu,Bing Liu,Wen-Guang Liu 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.4

no abstract

Two Novel 3-D Zinc Compounds with Mixture Ligands of H3BTB and 4,4'-Bipy: Crystal Structures, Blue Fluorescence and Single Point Energy Calculation

Zhang, Xiu-Cheng,Xu, Ling,Liu, Wen-Guang,Liu, Bing Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.9

Two 3-D zinc framework compounds, $[Zn_6(BTB)_4(4,4'-bipy)_4(H_2O)_4]{\cdot}9H_2O$ (1) and $[Zn_3(BTB)_2(4,4'-bipy)_2(H_2O)_2]{\cdot}5H_2O$ (2) ($H_3BTB$ = 1,2,3-benzenetricarboxylic acid, 4,4'-bipy = 4,4'-bipyridine), are obtained from the diffusion method and hydrothermal reaction respectively. Though 1 and 2 has the same coordination geometries of zinc atoms and coordination mode of $BTB^{3-}$, their 2-D layers are different: mirror symmetric layers in 1; parallel ones in 2, further connecting by 4,4'-bipy into 3-D frameworks. The hydrothermal reaction of 2 results in a more stable 3-D framework than the one in 1, which is supported by the single point energy calculations. 1 and 2 show similar blue fluorescence at 417 nm, which can be assigned to LMCT.

Built-in electric field and hydrostatic pressure effects on exciton states in a wurtzite (In,Ga)N/GaN coupled double quantum well

Wang Guang-Xin,Duan Xiu-Zhi 한국물리학회 2023 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.83 No.6

In this study, the pressure-dependent exciton binding energy and other optical properties (i.e., interband emission energy, emission wavelength, etc.) in a wurtzite (In,Ga)N-GaN coupled double quantum well structures are investigated theoretically under the built-in electric feld. Using a variational approach, numerical calculations are carried out within the efective mass approximation, considering the pressure dependence of efective mass, band-gap energy, and dielectric constants of GaN and InGaN materials. The results reveal that the increasing hydrostatic pressure causes a signifcant blue-shift in the emission wavelength, and a linear increase of the built-in electric feld strength, interband emission energy, and exciton binding energy. In addition, the built-in electric feld strength of middle-barrier layer (left- and right-well layers) increases (decreases) gradually with increasing the well width. The oscillator strength and exciton radiation decay time obviously depends on the hydrostatic pressure. It is hope that the above conclusions are helpful to providing the ideas about the optimization of optical properties of light-emitting devices fabricated GaN-based wurtzite coupled double quantum well.