Proline accumulation and transcriptional regulation of praline biothesynthesis and degradation in Brassica napus

( Xing Ning Xue ),( Ai Hua Liu ),( Xue Jun Hua ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.1

Identification of Specific Gene Modules in Mouse Lung Tissue Exposed to Cigarette Smoke

Xing, Yong-Hua,Zhang, Jun-Ling,Lu, Lu,Li, De-Guan,Wang, Yue-Ying,Huang, Song,Li, Cheng-Cheng,Zhang, Zhu-Bo,Li, Jian-Guo,Xu, Guo-Shun,Meng, Ai-Min Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Background: Exposure to cigarette may affect human health and increase risk of a wide range of diseases including pulmonary diseases, such as chronic obstructive pulmonary disease (COPD), asthma, lung fibrosis and lung cancer. However, the molecular mechanisms of pathogenesis induced by cigarettes still remain obscure even with extensive studies. With systemic view, we attempted to identify the specific gene modules that might relate to injury caused by cigarette smoke and identify hub genes for potential therapeutic targets or biomarkers from specific gene modules. Materials and Methods: The dataset GSE18344 was downloaded from the Gene Expression Omnibus (GEO) and divided into mouse cigarette smoke exposure and control groups. Subsequently, weighted gene co-expression network analysis (WGCNA) was used to construct a gene co-expression network for each group and detected specific gene modules of cigarette smoke exposure by comparison. Results: A total of ten specific gene modules were identified only in the cigarette smoke exposure group but not in the control group. Seven hub genes were identified as well, including Fip1l1, Anp32a, Acsl4, Evl, Sdc1, Arap3 and Cd52. Conclusions: Specific gene modules may provide better understanding of molecular mechanisms, and hub genes are potential candidates of therapeutic targets that may possible improve development of novel treatment approaches.

Experimental and Numerical Investigation of the Effect of Blast-induced Vibration from Adjacent Tunnel on Existing Tunnel

Hua-bing Zhao,Yuan Long,Xing-hua Li,Liang Lu 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.1

Since the new tunnel is close to existing tunnel, the vibration wave induced by blasting endangers the safety and stabilization of the surrounding rock and the lining of existing tunnel. In the tunnel blasting vibration monitoring and safety prediction, Peak Particle Velocity (PPV) and vibration frequency are used widely as safety standards. To investigate the effect of blast-induced vibration from adjacent tunnel on existing tunnel, field monitoring experiments and a numerical method that is Finite Element Method (FEM) were adopted to study the blasting vibration velocity and vibration frequency of existing tunnel. Combined blasting vibration velocity with vibration frequency, the paper studied axial and radial blasting vibration velocity distributions and the corresponding Power Spectral Density (PSD) distributions of the existing tunnel under the effect of blast-induced vibration from adjacent subway tunnel. And the parameters of constitutive model and blasting loads were also discussed. It is shown that field monitoring experiment and numerical simulation can optimize blasting excavation program and provide a reference for other similar engineering projects.

Associations of Plasma Glucagon Levels with Estimated Glomerular Filtration Rate, Albuminuria and Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus

Hua-Xing Huang,Liang-Lan Shen,Hai-Yan Huang,Li-Hua Zhao,Feng Xu,Dong-Mei Zhang,Xiu-Lin Zhang,Tong Chen,Xue-Qin Wang,Yan Xie,Jian-Bin Su 대한당뇨병학회 2021 Diabetes and Metabolism Journal Vol.45 No.6

Background: Type 2 diabetes mellitus (T2DM) is characterized by elevated fasting glucagon and impaired suppression of postprandial glucagon secretion, which may participate in diabetic complications. Therefore, we investigated the associations of plasma glucagon with estimated glomerular filtration rate (eGFR), albuminuria and diabetic kidney disease (DKD) in T2DM patients.Methods: Fasting glucagon and postchallenge glucagon (assessed by area under the glucagon curve [AUCgla]) levels were determined during oral glucose tolerance tests. Patients with an eGFR <60 mL/min/1.73 m2 and/or a urinary albumin-to-creatinine ratio (UACR) ≥30 mg/g who presented with diabetic retinopathy were identified as having DKD.Results: Of the 2,436 recruited patients, fasting glucagon was correlated with eGFR and UACR (r=–0.112 and r=0.157, respectively; P<0.001), and AUCgla was also correlated with eGFR and UACR (r=–0.267 and r=0.234, respectively; P<0.001). Moreover, 31.7% (n=771) presented with DKD; the prevalence of DKD was 27.3%, 27.6%, 32.5%, and 39.2% in the first (Q1), second (Q2), third (Q3), and fourth quartile (Q4) of fasting glucagon, respectively; and the corresponding prevalence for AUCgla was 25.9%, 22.7%, 33.7%, and 44.4%, respectively. Furthermore, after adjusting for other clinical covariates, the adjusted odds ratios (ORs; 95% confidence intervals) for DKD in Q2, Q3, and Q4 versus Q1 of fasting glucagon were 0.946 (0.697 to 1.284), 1.209 (0.895 to 1.634), and 1.521 (1.129 to 2.049), respectively; the corresponding ORs of AUCgla were 0.825 (0.611 to 1.114), 1.323 (0.989 to 1.769), and 2.066 (1.546 to 2.760), respectively. Additionally, when we restricted our analysis in patients with glycosylated hemoglobin <7.0% (n=471), we found fasting glucagon and AUCgla were still independently associated with DKD.Conclusion: Both increased fasting and postchallenge glucagon levels were independently associated with DKD in T2DM patients.

Application of mild local hyperthermia to treat skin diseases

( Xing-hua Gao ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

The Therapeutic Effects of Tectorigenin on Chemically Induced Liver Fibrosis in Rats and an Associated Metabonomic Investigation

Xing-Xi Gao,Jun-Hua Wu,Da-Hua Shi,Yun-Xi Chen,Jiang-Tao Cui,Yu-Rong Wang,Chun-Ping Jiang 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.8

The aim of this study was to investigate the effects of tectorigenin on chemically induced liver fibrosis in rats. Liver fibrosis was induced in rats with carbon tetrachloride, a diet high in fat, cholesterol and alcohol in the drinking water. Our results indicate that tectorigenin treatment significantly inhibited the increases in the activities of alanine aminotransferase (ALT),aspartate aminotransferase (AST) and the increases in the serum levels of hyaluronate (HA), laminin (LN) and procollagen III N-terminal peptide (PIIIP); tectorigenin treatment also significantly inhibited the increases in the amount of collagen in the livers of the fibrogenic rats. Chemically induced liver fibrosis caused a drop in the serum albumin concentration and a decrease in the ratio of albumin to globulin (A/G). Tectorigenin caused a remarkable increase at a dose of 30 mg/kg, but only a slight increase at the lower doses. Tectorigenin was also able to inhibit the increase in the liver lipid peroxidation (LPO), as well as the decrease in the activities of liver superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), caused by liver fibrosis. In addition, we present a related metabolic profile determined, using a 1H NMR spectroscopy and multivariate pattern recognition techniques. The results were consistent with the pathological examination, liver function analysis and liver fibrosis marker analysis. Furthermore, tectorigenin does not cause acute toxicity.

Highly Sensitive and Selective PbTiO₃ Gas Sensors with Negligible Humidity Interference in Ambient Atmosphere

Ma, Xing-Hua,Li, Hua-Yao,Kweon, Sang-Hyo,Jeong, Seong-Yong,Lee, Jong-Heun,Nahm, Sahn American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.5

<P>Three PbTiO<SUB>3</SUB> nanostructures were synthesized using a one-step hydrothermal reaction with different TiO<SUB>2</SUB> powders as Ti sources, and their gas-sensing properties were investigated. The sensor comprising PbTiO<SUB>3</SUB> nanoplates (NPs) exhibited a high response (resistance ratio = 80.4) to 5 ppm ethanol at 300 °C and could detect trace concentrations of ethanol down to 100 ppb. Moreover, the sensor showed high ethanol selectivity and nearly the same sensing characteristics despite the wide range of humidity variation from 20 to 80% RH. The mechanism for humidity-independent gas sensing was elucidated using diffuse reflectance infrared Fourier transform spectra. PbTiO<SUB>3</SUB> NPs are new and promising sensing materials that can be used for detecting ethanol in a highly sensitive and selective manner with negligible interference from ambient humidity.</P> [FIG OMISSION]</BR>

중국 국내 한국어 듣기 교재 연구 -제시대화문의 실질형태소 범주별 쓰임을 중심으로-

노성화 ( Xing Hua Lu ) 연세대학교 언어연구교육원 한국어학당 2012 외국어로서의 한국어교육 Vol.37 No.-

The Research of Korean listening textbooks in China -focuses on the category usage of full morpheme in the passages of dialogues-. Teaching Korean as a Foreign Language, 37, 125~138. The reliability of foreign language textbooks is generally reflected in three aspects, the true situation, the natural language and the variety of materials. This research focused on the naturalness of the language, using two corpus which were created by the author directly. Firstly, it had calculated the confidence interval of the full morpheme`s categorical usage which is in the annotated corpus of using in oral Korean morpheme in the interval estimation methods in statistics. Then, setting morphological annotated corpus in Korean listening textbooks as the object, examined the difference between the diametrical appearance rate of real morphemes which in each textbook and the categorical utilization`s confidence interval of oral Korean. The result of the comparison is that Korean listening textbooks which were published in China had some differences with real oral Korean in terms of categorical morphemes of full morphemes. The utilization of proper nouns, nouns, adjectives, verbs, auxiliary verbs, pre-nouns, conjunctive adverbs was higher than that in the oral Korean, but the utilization of pronouns, numerals, verbs, negative copular, proper adverbs, interjections was lower than the oral Korean. Therefore, because of the purpose of the dialogues which in Korean listening textbooks is for educational, we should not focus only on grammatical item or the amount of vocabulary, but provide plenty of opportunities that learners can access to natural conversations which are close to real life and adjust on the actual characteristics of the oral Korean. We should satisfy that the Korean learners` learning needs as much as possible.

중국의 한국어 듣기 교재 제시대화문에 대한 계량언어학적 연구 -수사의 쓰임을 중심으로-

노성화 ( Xing Hua Lu ) 연세대학교 언어연구교육원 한국어학당 2015 외국어로서의 한국어교육 Vol.42 No.-

As the source of language input, dialogue samples in listening textbook play a very important role in listening teaching. In this paper, we compared the numerals in dialogue samples and quasi oral using the interval estimation method of statistics. This paper includes two aspects, one is the study of numeral analogy, the other is the study of the words’ structure. In the first part, according to morphological characteristics, we divided the numerals into cardinals and ordinals, and then according to numerals’ sources, we divided them into 3 categories: inherent words system, Chinese character words system and loanwords system. Next, we analyzed 5 structures of words which accounted for 90.95% numerals. The results show that there are some differences in the use of numerals and words’ structure in Korean listening textbooks published in China. According to this result, we put forward some suggestions for how to write dialogue samples in Korean listening textbooks in the future. (LuoYang Foreign Language University of China · YanBian University of China)

Pituitary P62 deficiency leads to female infertility by impairing luteinizing hormone production

Li Xing,Zhou Ling,Peng Guiliang,Liao Mingyu,Zhang Linlin,Hu Hua,Long Ling,Tang Xuefeng,Qu Hua,Shao Jiaqing,Zheng Hongting,Long Min 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

P62 is a protein adaptor for various metabolic processes. Mice that lack p62 develop adult-onset obesity. However, investigations on p62 in reproductive dysfunction are rare. In the present study, we explored the effect of p62 on the reproductive system. P62 deficiency-induced reproductive dysfunction occurred at a young age (8 week old). Young systemic p62 knockout (p62 -/- ) and pituitary-specific p62 knockout (p62 flox/flox αGSU cre ) mice both presented a normal metabolic state, whereas they displayed infertility phenotypes (attenuated breeding success rates, impaired folliculogenesis and ovulation, etc.) with decreased luteinizing hormone (LH) expression and production. Consistently, in an infertility model of polycystic ovary syndrome (PCOS), pituitary p62 mRNA was positively correlated with LH levels. Mechanistically, p62 -/- pituitary RNA sequencing showed a significant downregulation of the mitochondrial oxidative phosphorylation (OXPHOS) pathway. In vitro experiments using the pituitary gonadotroph cell line LβT2 and siRNA/shRNA/plasmid confirmed that p62 modulated LH synthesis and secretion via mitochondrial OXPHOS function, especially Ndufa2, a component molecule of mitochondrial complex I, as verified by Seahorse and rescue tests. After screening OXPHOS markers, Ndufa2 was found to positively regulate LH production in LβT2 cells. Furthermore, the gonadotropin-releasing hormone (GnRH)-stimulating test in p62 flox/flox αGSU cre mice and LβT2 cells illustrated that p62 is a modulator of the GnRH-LH axis, which is dependent on intracellular calcium and ATP. These findings demonstrated that p62 deficiency in the pituitary impaired LH production via mitochondrial OXPHOS signaling and led to female infertility, thus providing the GnRH-p62-OXPHOS(Ndufa2)-Ca 2+ /ATP-LH pathway in gonadotropic cells as a new theoretical basis for investigating female reproductive dysfunction.