Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae

( Xing Yu Li ),( Zi Chao Mao ),( Yue Hu Wang ),( Yi Xing Wu ),( Yue Qiu He ),( Chun Lin Long ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.3

Bacillus subtilis XF-1, a strain with demonstrated ability to control clubroot disease caused by Plasmodiophora brassicae, was studied to elucidate its mechanism of antifungal activity against P. brassicae. Fengycin-type cyclopeptides (FTCPs), a well-known class of compounds with strong fungitoxic activity, were purified by acid precipitation, methanol extraction, and chromatographic separation. Eight homologs of fengycin, seven homologs of dehydroxyfengycin, and six unknown FTCPs were characterized with LC/ESI-MS, LC/ESI-MS/MS, and NMR. FTCPs (250μg/ml) were used to treat the resting spores of P. brassicae (107/ml) by detecting leakage of the cytoplasm components and cell destruction. After 12 h treatment, the absorbencies at 260 nm (A260) and at 280 nm (A280) increased gradually to approaching the maximum of absorbance, accompanying the collapse of P. brassicae resting spores, and nearly no complete cells were observed at 24 h treatment. The results suggested that the cells could be cleaved by the FTCPs of B. subtilis XF-1, and the diversity of FTCPs was mainly attributed to a mechanism of clubroot disease biocontrol.

Whitening and inhibiting NF- B-mediated infl ammation properties of the biotransformed green ginseng berry of new cultivar K1, ginsenoside Rg2 enriched, on B16 and LPS-stimulated RAW 264.7 cells

Xing Yue Xu,Eun Seob Yi,Chang Ho Kang,Ying Liu,Yeong-Geun Lee,Han Sol Choi,Hyun Bin Jang,Yue Huo,Nam In Baek,Deok Chun Yang,Yeon Ju Kim 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.6

Background: Main bioactive constituents and pharmacological functions of ripened red ginseng berry(Panax ginseng Meyer) have been frequently reported. Yet, the research gap targeting the beneficial activitiesof transformed green ginseng berries has not reported elsewhere. Methods: Ginsenosides of new green berry cultivar K-1 (GK-1) were identified by HPLC-QTOF/MS. Ginsenosidesbioconversion in GK-1 by bgp1 enzyme was confirmed with HPLC and TLC. Then, mechanismsof GK-1 and b-glucosidase (bgp1) biotransformed GK-1 (BGK-1) were determined by QuantitativeReverse Transcription-Polymerase Chain Reaction and Western blot. Results: GK-1 possesses highest ginsenosides especially ginsenoside-Re amongst seven ginseng cultivarsincluding (Chunpoong, Huangsuk, Kumpoong, K-1, Honkaejong, Gopoong, and Yunpoong). Ginseng root’sbiomass is not affected with the harvest of GK-1 at 3 weeks after flowering period. Then, Re is bioconvertedinto a promising pharmaceutical effect of Rg2 via bgp1. According to the results of cell assays,BGK-1 shows decrease of tyrosinase and melanin content in a-melanocyte-stimulating hormonechallenged-murine melanoma B16 cells. BGK-1 which is comparatively more effective than GK-1 extractshows significant suppression of the nuclear factor (NF)-kB activation and inflammatory target genes, inLPS-stimulated RAW 264.7 cells. Conclusion: These results reported effective whitening and anti-inflammatory of BGK-1 as compared toGK-1.

Anatomical Study of the Accessory Tendon of the Extensor Hallucis Longus Muscle and Its Clinical Application

Yue Li,Jing-Ying Zhang,Xin-Yue Zhao,Li-Ya Pan,De-Hao Jin,He-Xing Xu,Hu-Zhe Cui,Yan-Qun Liu,Xiang-Zheng Qin,Qingyuan Li 대한정형외과학회 2021 Clinics in Orthopedic Surgery Vol.13 No.2

Background: The accessory tendon of the extensor hallucis longus (ATEHL) muscle is a common abnormal structure, and its clinical significance remains debatable. In this study, we provide the incidence of the ATEHL and characterize its morphological types in Asian cadavers and investigate its clinical applications. Methods: The tendons from 50 adult cadaveric feet, fixed in 10% formalin, were analyzed. We measured the length and width of both the ATEHL and the extensor hallucis brevis (EHB). Results: All dissected specimens had an ATEHL. The first metatarsophalangeal joint was surrounded by an accessory tendon that inserted onto the joint capsule and the dorsal base of the proximal phalanx. We classified the ATEHL into 3 types based on their directions. Differences in ATEHL type based on sex were not statistically significant. Conclusions: We found an ATEHL in all cadaveric specimens in this study. We surmise that the ATEHL acts as an antagonist with the EHB when the toe is extending, which might help prevent the occurrence of hallux valgus deformity.

Influence of Autologous and Homologous Blood Transfusion on Interleukins and Tumor Necrosis Factor-α in Peri-operative Patients with Esophageal Cancer

Xing, Yue-Li,Wang, Yan-Chun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Objective: To explore the influence of different ways of blood transfusion on the expression levels of interleukins (IL) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) inperi-operative patients with esophageal cancer. Materials and Methods: A total of 80 patients with esophageal cancer who underwent radical operations were selected as study patients and randomly divided into an observation group (treated with autologous blood transfusion) and control group (with homologous blood transfusion). Changes of intra-operative indexes and peri-operative blood indexes, from hemoglobin (Hb) and hematocrit value (Hct), to levels of inflammatory factors like interleukins-6 (IL-6), IL-8, IL-10 and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) were compared. Results: Operations for patients in both groups were successfully conducted, and no significant differences in mean surgical duration and intra-operative hemorrhage volume, fluid infusion volume and blood transfusion volume were detected (p>0.05). Compared with values before surgery, Hb and Hct levels decreased significantly while white blood cell count (WBC) increased 1, 5 and 7 d after operation (p<0.05, p<0.01). In addition, WBC was apparently higher in observation group than in control group 5 and 7 d after operation (p<0.01). Compared with before surgery, in the observation group, levels of IL-6, IL-8 and IL-10 had no significant differences after operation (P>0.05), but TNF-${\alpha}$ level increased y (p<0.01), whereas in control group, IL-6 level had no significant difference (p>0.05), IL-8 level decreased obviously (p<0.05), IL-10 level increased markedly first and then decreased gradually as time passed but its level remained elevated (p<0.01), and TNF-${\alpha}$ level increased first and then decreased, and there was no significant difference 7 d after operation (p>0.05). Conclusions: Decreased IL-8 and increased IL-10 levels are two important reasons for immunosuppression after homologous blood transfusion, whereas autologous blood transfusion can alleviate this while increasing the TNF-${\alpha}$ level, which also has potential to improve anti-tumor immunity in the human body.

중국 노후 주거지역 도시재생을 위한 CPTED 요소분석

형월(Xing Yue),문정민(Moon,Jeong Min) 한국디자인리서치학회 2021 한국디자인리서치 Vol.6 No.3

중국의 급속한 도시화 진행에 따라 대량의 노후 주거지역이 형성되고, 노후 주거지역에서 발생하는 각종 범죄가 갈수록 증가하고 있다. 도시의 높은 범죄율은 CPTED를 통한 도시재생으로 범죄율을 낮출 필요가 있다. 현재 중국은 한국에 비해 CPTED 연구가 비교적 늦고, 이론과 기술의 접점이 부족해 연구 방법이 단일하다. 본 연구는 우선 선행연구를 통해, 한국 18편과 중국 15편의 노후 주거지역 재생 사례 관련 분석논문과 CPTED의 주요 6요소 운용실태를 분석한 다음 위 논문의 CPTED의 주요 상세 요소의 내용을 분석한다. 주요 요소를 시설물, 물리적 공간과 운영, 유지관리 등 크게 3개 부문으로 나누며, 6개의 세부 상세 요소를 도출한다. 자연적 감시에서는 한국은 45개, 중국 23개, 접근통제영역은 한국 17개, 중국 15개. 영역성 부분은 한국 21개, 중국 18개. 활동지원에서는 한국 23개, 중국1 2개. 유지관리 부분은 한국 28개, 중국16개, 주민참여에서는 한국 10개, 중국 2개이다. 따라서 이 연구의 목적은, 최종적으로 한국·중국 요소의 세부기준별 비교를 통해 한.중 연구의 차별성을 제시함으로써, 중국 노후 주거지역 재생의 시사점을 찾는 데 있다. With the rapid urbanization of China, a large number of old residential areas is formed, and various crimes occurring in old residential areas is increasing. The high crime rate in cities needs to be lowered through urban regeneration through CPTED. Currently, China is slower in CPTED research than Korea and lacks combination of theory and real situation, so research methods are not diverse. As part of previous studies, this one first analyzed 18 Korean papers and 15 Chinese papers related to regeneration cases in old residential areas in China, focusing on the operation status of CPTED 6 factors. Subsequently, the details of CPTED elements in these papers were analyzed and divided into three categories: facility, physical space with operation, and maintenance. And the number of papers are as followings; 45 Korean papers and 23 Chinese papers on natural monitoring, 17 Korean papers and 15 Chinese papers on direct control, 21 Korean papers and 18 Chinese papers on areal part, 23 Korean papers and 12 Chinese ones on activity support, 28 Korean and 16 Chinese papers on maintenance, and 10 Korean and 2 Chinese on resident participation. Finally, it is believed that the implications of the regeneration of deteriorated residential areas in China can be found by presenting the differences in research between Korea and China through comparison of detailed standards of Korean and Chinese factors.

중국 선전, 광저우의 노후주거지역 도시재생을 위한 공간인지 분석

형월 ( Xing Yue ),문정민 ( Jeong Min Moon ) 한국디자인트렌드학회 2021 한국디자인포럼 Vol.26 No.1

연구배경 중국의 도시재생은 개혁개방 이래로 40년 동안 발전과정을 거쳐 온 결과, 대도시 중심의 도시재생을 통해 새로운 도시재생 형식과 수단이 등장했다는 점에서 발전적 의미를 갖는다. 그러나 정부의 도시재생은 전반적으로 역사문화거리 및 중공업 건물 등 대규모 단위 재생에 그 초점이 맞춰져 있어 노후주거지역의 도시재생은 점차 관심에서 소외되었다는 점에서 한계를 가지고 있다. 연구방법 인지 심리학을 배경으로 필자가 제시한 공간인지 5요소를 이론적 틀로 하여, 선전, 광저우의 노후 주거지역의 도시 재생을 조사·분석하였다. 연구결과 첫째, 공간인지 5요소의 경로, 경계, 노드, 구역과 랜드마크가 노후 주거지역에서 결여되거나 일부 결여되어 안전문제와 환경문제가 발생한다는 사실을 밝혀냈기 때문에 인지지도의 구성은 매우 중요하다. 둘째, 공간인지요소이론에 기초한 도시재생을 통해 광저우, 선전 노후 주거지역의 도시재생을 분석하였을 때, 노후 주거지역의 공간인지5요소가 부분적으로 결여되어 도시재활에 문제가 있다는 연구결과가 나오게 되므로 환경 개선이 필요하다. 결론 본 연구에서는 공간인지 5요소의 부족이 도시재생에 미치는 영향을 밝힘으로써 그 필요성을 증명했다. 주민들의 주거지역에서 완성도 높은 공간인지는 환경설계에 영향을 미치며, 나아가 거주민들에게 안정감을 준다. 또한 무분별한 개발보다는 원주민의 주거환경과 지역이 가진 역사성을 보존하여 지속가능한 발전이 가능하도록 해야 한다. Background China's urban regeneration practice has been developing since the reform and opening up to the outside world for 40 years. Despite the appearance of new forms and means in some large cities, the urban regeneration still remains in the center of government work and the renewal of old industrial buildings, neglecting the urban regeneration in the old residential areas. Methods With the background of cognitive psychology, the five factors of spatial perception suggested by the author were used as the theoretical framework, and the urban regeneration of the old residential areas of Shenzhen and Guangzhou was investigated and analyzed. Result First, the cognitive map construction is very important because the cognitive map's five elements of path, boundary, node, area and logo are missing or partially missing in the old residential areas. Secondly, the paper analyzes the urban regeneration of the old residential areas by using the theory of cognitive factors, and study shows that there are partial deficiencies of cognitive factors in those areas, so there are problems of urban regeneration and the environments there still needs to be improved. Conclusion This study has shown that the lack of spatial cognition factors has a bright effect on urban regeneration, proving its necessity. The high degree of completion of the residential areas will have an impact on the design of the environment, thus giving the residents a sense of security. In addition, it is more important to protect the living environments and the historic nature of the areas than to develop blindly, so that it can continue to grow.

從民間到政府主導的邊緣居住區都市再生硏究 -以深圳市大芬村文化創意産業融合轉型爲例

형월(Xing Yue),박재연(Park, Jae Yeon) 한국전시산업융합연구원 2020 한국과학예술융합학회 Vol.38 No.2

This thesis takes the urban regeneration and industrial integration transformation of urban village Dafen in Shenzhen as the research object, and takes the urban renewal theory as the research framework to analyze the industrial transformation and rural spatial evolution under the leading driving force of Dafen Village renewal in different periods. This thesis explores the relationship between the development of Dafen Village"s cultural and creative industries and the evolution of rural space, sorts out the government driven urban renewal mode of Dafen Village from high imitation to original art industry transformation, clarifies the special value of "Natural Village、High imitation Village、 Art Village" in the process of industrial transformation under the leading driving force of folk and government, sum up its transformation mode and pattern. According to statistics, there are tens of thousands of high imitation villages in China at present. This thesis proposes a solution model for the future development of other high imitation villages in China, which has universal applicability value.

제8회 한,중 형법 국제 학술심포지엄 : 윤리의 변천과 형법의 변혁

쩡외씽 ( Yue Xing Zeng ) 한국비교형사법학회 2010 비교형사법연구 Vol.12 No.2

제7회 한증 형법 국제 학술심포지움 : 항저우 차량폭주사건의 법리분석

졍웨씽 ( Zeng Yue Xing ),왕샤오훼이 ( Wang Xiao Hui ) 한국비교형사법학회 2009 비교형사법연구 Vol.11 No.2

Experimental research and molecular dynamics simulation on thermal properties of capric acid/ethylene-vinyl/graphene composite phase change materials

Yue Gu,Linhua Jiang,Weizhun Jin,Zhenhua Wei,Xing Liu,Mingzhi Guo,Kailun Xia,Lei Chen 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.99 No.-

In this paper, a new series of phase change materials (PCMs) composed of capric acid/ethylene-vinylacetate/graphene (CA/EVA/GR) were prepared and thermal properties were investigated using moleculardynamics simulation. The composite PCMs were characterized by Fourier transform infraredspectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), differential scanningcalorimetry (DSC) and thermal conductivity measurement. FT-IR, XRD and SEM results manifest that CAcan be successfully wrapped by EVA and GR additives, and there is no chemical reaction between CA, EVAand GR. DSC results indicate that adding GR into CA/EVA can result in composite PCMs maintain the highlatent heat, while too much GR will cause a significant reduction in latent heat. Thermal conductivityobtained from experimental tests reveal that GR can gradually enhance the thermal conductivity of CA/EVA with increasing dosage of GR. The experimental results of thermal conductivity fall close to that ofmolecular dynamics (MD) simulation at GR dosages below 1.8 wt%, but the experimental results present atrend which is contrary to MD simulation at higher GR dosages. The mean square displacement (MSD)results manifest that composite PCMs containing 1.8 wt% GR has the highest diffusion coefficient, whilehigher GR dosage will reduce the diffusion coefficient gradually.