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Xiaohua Feng,Qianbing Zhang,Songxin Xia,Bing Xia,Yue Zhang,Xubin Deng,Wenmei Su,Jianqing Huang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.9
The metastasis-associated gene 1 (MTA1) oncogene hasbeen suggested to be involved in the regulation of cancer progression. However, there is still no direct evidence that MTA1 regulates cisplatin (CDDP) resistance, as well as cancer stem cell properties. In this study, we found that MTA1 was enriched in CNE1/CDDP cells. Knock down of MTA1 in CNE1/CDDP cells reversed CSCs properties and CDDP resistance. However, ectopic expression of MTA1 in CNE1 cells induced CSCs phenotypes and CDDP insensitivity. Interestingly, ectopic overexpression of MTA1-induced CSCs properties and CDDP resistance were reversed in CNE1 cells after inhibition of PI3K/Akt by LY294002. In addition, MTA1 expression and Akt activity in CNE1/CDDP cells was much higher than that in CNE1 cells. These results suggested that MTA1 may play a critical role in promoting CDDP resistance in NPC cells by regulatingcancer stem cell properties via thePI3K/Akt signaling pathway. Our findings suggested that MTA1 may be a potential target for overcoming CDDP resistance in NPC therapy.
Design of the energy-balanced wireless sensor networks for 3D seismic exploration
Xiaohua Zhou,Jianbin Zheng,Xinkai Meng,Guanghui Sun,Ruyun Tian 한국통신학회 2023 ICT Express Vol.9 No.3
Wireless Sensor Networks for 3D Seismic Exploration are large-scale and long-term networks to ensure high resolution. Energy balance is essential to avoid interruption of the whole network. In this paper, the hybrid wireless network architecture is designed for low-power monitoring system. An energy-balanced clustering method is developed to prolong network lifetime. The partial energy factor is introduced to optimize scheduling of cluster head nodes. An improved ant colony algorithm for energy-efficient clustering and routing network (IACA-EECR) is proposed to find optimal path. The results show the proposed architecture outperforms the existing platform. Extensive tests validate energy efficiency and network performance.
Xiaohua, Jiang,Zhiwen, Lv,Wenjie, Ding,Ying, Zhang,Feng, Lin The Korean Electrochemical Society 2022 Journal of electrochemical science and technology Vol.13 No.4
As one of the most hazardous and deadliest pathogens, Listeria monocytogenes (LM) posed various serious diseases to the human being, thus designing effective strategy for its detection is of great significance. In this work, by preparing Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXenes nanoribbon (Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>R) as carrier and selecting thionine (Th) acted simultaneously as signal probe and functional monomer, a LM pathogen-imprinted polymers (PIP) integrated probe electrochemical sensor was design to monitor LM for the first time, that was carried out through the electropolymerization of Th on the Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>R/GCE surface in the existence of LM. Upon eluting the templates from the LM imprinted cavities, the fabricated PIP/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>R/GCE sensor can rebound LM cells effectively. By recording the peak current of Th as the response signal, it can be weakened when LM cell was re-bound to the LM imprinted cavity on PIP/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>R/GCE, and the absolute values of peak current change increase with the increasement of LM concentrations. After optimizing three key parameters, a considerable low analytical limit (2 CFU mL<sup>-1</sup>) and wide linearity (10-10<sup>8</sup> CFU mL<sup>-1</sup>) for LM were achieved. In addition, the experiments demonstrated that the PIP/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>R sensor offers satisfactory selectivity, reproducibility and stability.
Xiaohua Zhao,Dunli Hu,Xinyu Yang,Xuewei Li,Xiaofan Feng,Jiaxia Yang 한국자동차공학회 2022 International journal of automotive technology Vol.23 No.4
To further enhance the role of connected vehicles with the Human-Machine Interface (HMI) in helping drivers in foggy weather, it is meaningful to optimize the HMI based on the driver’s needs. In order to explore the method of HMI optimization, this paper builds a driving simulation experimental test platform of freeway connected vehicle system, designing two experimental scenarios according to the technical conditions (without HMI or with HMI). After that, the paper uses Markov chain to explore the drivers’ fixation transition to identify the driver’s needs in different sections at different conditions. Besides, combined with the visual trajectory results, the paper provides suggestions for optimizing HMI. The results show the review rate of drivers in each section for the straight upper front area is very high. The highest value occurs in the heavy fog zone, suggesting that HMI can provide more road information. HMI should offer a prompt to the driver before entering the warning zone, and remind the driver of changes in the speed limit before entering the fog area. The prompt module of HMI should shorten the horizontal length. In conclusion, this paper aims to propose a general diagnosis method by diagnosing the self-designed HMI.
Mechanical plugging—solid expandable tubular refracturing technology
Xiaohua Zhu,Feilong Cheng,Changshuai Shi,Kailin Chen 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.6
To solve the three major problems of low production, low efficiency and low permeability of oil fields, it is necessary to develop new tight oil layers or shale oil outside the original perforated section in the well that has been perforated. The key to the success of repeated fracturing operations is the ability to block the original perforation of the well. At present, with tubing fracturing construction, volume fracturing of the target reservoir cannot be achieved due to limited displacement, and the effect of reservoir transformation is not obvious, affecting the productivity of the old well. Based on the above problems, this paper proposes a new blocking method suitable for fracturing wells - solid expansdable tubular (SET) plugging. In this paper, it established SET-expansion cone-casing-rubber ring 2D dynamic model which considers the friction, and contact, to analyze the effect of compression and length of rubber ring and the constraint condition of SET on sealing performance; laboratory experiments were carried out and data on driving force, sealing capacity and suspension force were obtained. The results show that optimizing the parameters of expansion cone structure can effectively reduce the residual stress of SET and driving force after expansion; the construction method of SET and the parameters of rubber ring are the main factors that affect the suspension sealing ability, and paper proposes an effective solution; in addition, experiment results show that the SET meets the requirements of fracturing construction with internal pressure greater than 60 MPa and suspension force greater than 600 kN after expansion, and can be used to seal the well body structure of refracturing wells with fewer perforations and shorter distances, and the diameter can be guaranteed to reach 112 mm. The research results provide new ideas and solutions for repeated pressure wells and high-pressure plugging.
Design and analysis of sealed suspension module based on solid expandable tubular repair technology
Xiaohua Zhu,Feilong Cheng,Changshuai Shi,Jia’nan Li,Kailin Chen 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.2
The ability of sealing suspension of solid expandable tubular (SET) is one of the important factors that determine whether pipe can expand normally and serve. To solve the problem that the SET deviates from the original position (causing for the failure to repair of leakage well) due to the insufficient suspension force after expansion, this paper establishes the two-dimensional coupling model of SET-rubber ring-expansion cone-casing based on laboratory experiment parameters, designs the parameters of the sealed suspension rubber ring and conducts simulation. The results show that when the compression amount of the rubber ring is designed to be 47 %-58 % of the rubber thickness, the SET has excellent sealing ability after expansion and can meet the requirements of suspension force; too much compression will affect the service life of the rubber ring. When the length of the rubber ring is 50 mm-110 mm, the driving force and contact pressure during expansion are suitable; the rubber ring spacing is designed to be 110 mm, which avoids interference after rubber compression and saves material. In addition, the vulcanizing bonding strength of the rubber ring should be greater than 120 kN to avoid peeling off due to the failure of the rubber ring and SET bonding. The research results of this paper provide a theoretical basis for the design of sealing suspension module of SET repair technology.
Xiaohua Lu,Jianguo Lin,Changgao Wang,Xin Du,Jun Cai 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5
The purification and characterization of the extracellular polygalacturonase from Zygoascus hellenicus V25 submerged culture using orange peel waste were investigated. This polygalacturonase, with a molecular weight of 75.28 kDa, was purified to 16.89 purification fold with a recovery of 18.46% and specific activity of 2469.77 U/mg protein by ammonium sulfate precipitation, DEAE cellulose chromatography, and Sephadex G-100 gel filtration. The enzyme exhibited maximum activity at 60oC and pH 5.0 and was stable over a wide range of pH levels (3.0-11.0). Moreover, enzyme activity was enhanced by Cu2+ and cysteine, whereas it was strongly inhibited by Hg2+. The extent of enzymatic hydrolysis was negatively correlated with the degree of pectin esterification. Km and Vmax values of the polygalacturonase were 5.44 mg/mL and 61.73 μmol/(min·mg), respectively. The polygalacturonase was applied in the juice clarification of four fruits, and results showed that the percentage transmittance at 660 nm increased by 3.51, 4.36, 8.04, and 12.2%.