Identification of differentially expressed immunity‐related genes in Monochamus alternatus Hope (Coleoptera: Cerambycidae) larvae parasitized by Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae)

Xiao‐Juan Li,Guang-Ping DONG,Jian-Min FANG,Hong-Jian LIU,Wan-Lin GUO 한국곤충학회 2018 Entomological Research Vol.48 No.4

The pine sawyer Monochamus alternatus Hope (Coleoptera: Cerambycidae) is a serious pest of several Pinus species, and the ectoparasitoid larvae of Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an important natural enemy of this pest. The transcriptome of M. alternatus larvae was sequenced using the Illumina platform and immunity‐related genes were specifically analyzed. De novo assembly resulted in the identification of 24 241 unigenes, with a mean length of 1122 bp, in unparasitized M. alternatus larvae and 23 807 unigenes, with a mean length of 1140 bp, for parasitized larvae. Removal of redundant unigenes resulted in 26 095 all‐unigenes, of which 16 959 (64.99%) showed clear homology with some of the known genes in the National Center for Biotechnology Information nr database. Parasitization had notable effects on the transcriptome profile of M. alternatus larvae. In all, 2702 genes were differentially expressed in M. alternatus larvae after parasitization, with 1491 (55.18%) upregulated and 1211 (44.82%) downregulated. Moreover, expression levels of immunity‐related genes in M. alternatus larvae were markedly altered in response to parasitization by D. helophoroides. In conclusion, the transcriptome profiling data, especially the discovered of immunity‐related genes, help illustrate the molecular mechanisms of parasitism between D. helophoroides and M. alternatus and provide new insights into developing immunity regulation‐mediated control methods of M. alternatus.

Comparison of volatile organic compounds from uninfested and Monochamus alternatus Hope infested Pinus massoniana Lamb.

Xiao‐Juan Li,Guang-Ping DONG,Jian-Min FANG,Hong-Jian LIU,Wan-Lin GUO 한국곤충학회 2017 Entomological Research Vol.47 No.3

Monochamus alternatus is a destructive stem‐boring herbivore of Pinus massoniana, and the principal vector of pine wood nematode. To investigate the impacts of boring by M. alternatus larvae on the emission of volatile organic compounds (VOCs) from their host trees, the VOCs from uninfested and M. alternatus larvae infested P. massoniana trees were observed using a gas chromatograph–mass spectrometer. We detected 12, 9, 18 and 14 volatile organic compounds from infested xylem, infested phloem, uninfested xylem and uninfested phloem, respectively. In P. massoniana xylem, the boring of M. alternatus larvae induced cyclosativene, and inhibited 4‐carene, humulene, styrene, α‐phellandrene, β‐myrcene, β‐phellandrene and γ‐terpinene. The relative amounts of camphene, copacamphene, longicyclene, longifolene, tricyclene and α‐longipinene were significantly increased, and the relative amounts of α‐pinene and β‐pinene were significantly decreased by the boring behaviors of M. alternatus larvae. In P. massoniana phloem, the boring of M. alternatus larvae induced 2‐bornanone, copacamphene, longicyclene and α‐longipinene, and inhibited 2‐carene, 4‐carene, styrene, α‐phellandrene, β‐myrcene, β‐phellandrene, β‐pinene, γ‐terpinene and ο‐cymene. The relative amounts of camphene, caryophyllene and longifolene were significantly increased by the boring behaviors of M. alternatus larvae. The results indicate that the boring behaviors of M. alternatus larvae changed both the sorts and contents of the VOCs from P. massoniana trees.

Some Further Results on Weighted Sharing of Values for Meromorphic Functions Concerning a Result of Terglane

Li, Xiao-Min,Yi, Hong-Xun Department of Mathematics 2008 Kyungpook mathematical journal Vol.48 No.3

In this paper, we deal with the problem of meromorphic functions that have three weighted sharing values, and obtain some uniqueness theorems which improve those given by N. Terglane, Hong-Xun Yi & Xiao-Min Li, and others. Some examples are provided to show that the results in this paper are best possible.

Characteristics and Impact Factors of Renal Threshold for Glucose Excretion in Patients with Type 2 Diabetes Mellitus

Xiao-Dan Yue,Jing-Yu Wang,Xin-Rong Zhang,Ju-Hong Yang,Chun-Yan Shan,Miao-Yan Zheng,Hui-Zhu Ren,Yi Zhang,Shao-Hua Yang,Zhen-Hong Guo,Bai Chang,Bao-Cheng Chang 대한의학회 2017 Journal of Korean medical science Vol.32 No.4

Sodium glucose co-transporter 2 (SGLT-2) inhibitors are newly developed but promising medicine for type 2 diabetes. However, patients with a different renal threshold for glucose excretion (RTG) may have a different reaction to this medicine. Therefore, the objective of this study was to investigate the characteristics of RTG and its impact factors in patients with type 2 diabetes mellitus (T2DM). The clinical and laboratory data of 36 healthy individuals and 168 in-hospital patients with T2DM were collected and analyzed, RTG was calculated using blood glucose (BG) measured by dynamic BG monitoring, urinary glucose excretion (UGE) and estimated glomerular filtration rate (eGFR). The characteristics of RTG were investigated. The risk factors for high RTG were analyzed using non-conditional logistic regression analysis. Our results found that RTG of the T2DM group was higher than that of the healthy individuals (P < 0.05); and 22.22% from the healthy individuals group but 58.33% from the T2DM group had high RTG. Age, duration of diabetes, body mass index (BMI), and homeostasis model assessment insulin resistance index (HOMA-IR) were independently associated with high RTG (P < 0.05). Further stratified analysis revealed that RTG in T2DM patients increased with age, duration of diabetes, and BMI. In conclusion, RTG is increased in patients with T2DM, especially in those with longer diabetic duration, higher BMI, and those who are older. Therefore, these patients may be more sensitive to SGLT-2 inhibitors.

Intermedins A and B; New Metabolites from Schisandra propinqua var. intermedia

Hong-Mei Li,Chun Lei,Yong-Ming Luo,Xiao-Nian Li,Xiao-Lei Li,Jian-Xin Pu,San-Yun Zhou,Rong-Tao Li,Han-Dong Sun 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

A new dibenzocyclooctadiene lignan, intermedin A (1), and a new natural bisabolane sesquiterpenoid, intermedin B (2), were isolated from the aerial parts of Schisandra propinqua var. intermedia. Their structures were elucidated on the basis of extensive spectroscopical analysis.

Peniciside, a New Triterpenoid Glycoside, from the Fungus Penicillium sp. 169

Xiao-Hong Yuan,Guo-You Li,Guo-Bo Xu,Wei-Lin Wu,Tao Yang 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.2

Peniciside, a new fernene triterpenoid glycoside, was isolated from the EtOAc extract of the solid-state fermented rice culture of the fungus Penicillium sp. 169. Its structure was elucidated on the basis of spectroscopic analysis, and the absolute configuration was determined by X-ray crystallographic analysis and chemical methods. Peniciside is the first example of a fernene triterpenoid glycoside with two hydroxyls at C-19 and C-20.

Therapeutic Effect of Ginsenoside Rd in Rats with TNBS-Induced Recurrent Ulcerative Colitis

Xiao-Lai Yang,Yong-Jie Wu,Tian-Kang Guo,Yan-Hong Wang,Ming-Tang Gao,Hong Qin 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.7

Ulcerative colitis (UC) is characterized by oxidative and nitrosative stress and neutrophil infiltration. In the present study, we aimed to investigate the therapeutic effect of ginsenoside Rd (GRd) in rats with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced recurrent UC. After UC was twice-induced by intracolonic injection of TNBS, rats were intragastrically administered different doses of GRd per day for 7 days. The colonic lesions and inflammation were evaluated both histologically and biochemically. Compared with the TNBS group, GRd treatment facilitated recovery of pathologic changes in the colon after induction of recurrent UC, as evidenced by a significant reduction of colonic weight/length ratio and macroscopic and microscopic damage scores (p < 0.01). The myeloperoxidase and inducible nitric oxide synthase activities with malonyldialdehyde and nitric oxide levels in colonic tissues were significantly decreased in the GRd group compared with those in the TNBS group (p < 0.01). GRd treatment was associated with remarkably increased superoxide dismutase and glutathione peroxidase activities. Results showed a valuable effect of GRd against TNBS-induced recurrent UC by inhibiting neutrophil infiltration and promoting the antioxidant capacity of the damaged colonic tissue.

Inhibition of Breast Cancer Metastasis Via PITPNM3 by Pachymic Acid

Hong, Ri,Shen, Min-He,Xie, Xiao-Hong,Ruan, Shan-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Breast cancer metastasis is the most common cause of cancer-related death in women. Thus, seeking targets of breast tumor cells is an attractive goal towards improving clinical treatment. The present study showed that CCL18 from tumor-associated macrophages could promote breast cancer metastasis via PITPNM3. In addition, we found that pachymic acid (PA) could dose-dependently inhibit migration and invasion of MDA-MB-231cells, with or without rCCL18 stimulation. Furthermore, evidence was obtained that PA could suppress the phosphorylation of PITPNM3 and the combination of CCL18 and PITPNM3. Therefore, we speculate that PA could inhibit breast cancer metastasis via PITPNM3.

Optimization Scheme of Genetic Algorithm and Its Application on Aeroengine Fault Diagnosis

Hong Xiao,Zhe-Zhu Xu,김래성,Dong-Yang Li,류성기 한국정밀공학회 2015 International Journal of Precision Engineering and Vol.16 No.4

An adaptive model is proposed based on genetic algorithm to predict the characteristic map of aeroengine components. The differencefunctions, of the primary performance parameters between numerical model and test data, are taken as objective function. Thecoupled factors of component characteristics' map as optimized parameters are considered.The difference of the main performanceparameters and process parameters between the adaptive model and the test data are shown to be within the range of0.05%.Meanwhile, the section's total temperature and pressure are controlled within 1%. Furthermore, an aerongine fault diagnosismodel is developed by the small deviation equation method in which the gas path analysis is implemented and the symptom andmeasuring parameters represent engine performance parameters' variation. It shows that the selection and relatively variable valueof symptom parameter have great effect on fault diagnosis error, and the best selection of value is 1/3 of threshold. The relative errorof variable value between the symptom parameter of fault diagnosis model and the real fault can be found to be controlled within5% and it can do the correct evaluation of fault type. And the fault diagnosis model has no misdiagnosis in all the performedconditions.

Experimental Research on a Hypersonic Configuration with Blunt Forebody Edges

Xiao, Hong,Xu, Zhe-Zhu,Kim, Lae-Sung,Li, Dong-yang,Lyu, Sung-Ki Korean Society for Precision Engineering 2015 International Journal of Precision Engineering and Vol.16 No.10

A design method, for hypersonic forebody configuration with blunt edges, was developed by lift body and waverider. Experimental study was conducted. It was showed that the difference of pressure coefficient, at centre-line and side-line on forebody pre-compression surface, was between 2.5% and 16.7% within the Mach number 5 to 7 and attack angle 0 to 12. These differences were slightly bigger than those of non-blunt-edges forebody. However, experimental data also show that the present configuration can achieve the closed shockwave, and can also realize the automatic overflow of boundary layer on forebody pre-compression surface. It is also observed that the present forebody gets a good aerodynamic performance at high Mach numbers. But with increasing attack angle, the advantage was weakened gradually. And, at the degree of side-slip exceeding 8, the side-line pressure is higher than that of center-line, which indicates that the forebody boundary layer does not achiev automatically overflowing to side surface.