Expression and Significance of the Wip1 Proto-oncogene in Colorectal Cancer

Li, Zong-Tao,Zhang, Liu,Gao, Xiao-Zeng,Jiang, Xiao-Hua,Sun, Li-Qian Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Aim: To investigate the level of expression of proto-oncogene Wip1 and its physiological significance in colorectal cancer. Methods: Immunohistochemistry, semi-quantitative RT-PCR, and Western blotting were used to analyze Wip1 mRNA and protein expression in 120 cases of colorectal cancer and normal tissues to study relationships with clinical symptoms and disease prognosis. Results: The level of Wip1 protein expression was found to be significantly higher in colorectal cancer tissues (85% (102/120)) than in normal tissues (30% (36/120)) (P<0.05). The relative amount of Wip1 protein in colorectal cancer tissue was also found to be significantly higher (P<0.05) than in normal tissues ($1.060{\pm}0.02$ and $0.640{\pm}0.023$, respectively). Semi-quantitative RT-PCR showed average Wip1 mRNA expression levels to be $1.113{\pm}0.018$ and $0.658{\pm}0.036$ for colorectal cancer tissue and adjacent normal tissue (P<0.05). The level of Wip1 protein expression was not correlated with age, gender, or tumor site, but appeared linked with lymph node metastasis, Dukes stage, histological grade, and liver metastasis. Individuals with high and low levels of Wip1 expression showed statistically significant differences in the five-year overall survival and recurrence-free survival rates (P<0.05). Conclusion: Wip1 mRNA and protein are highly expressed in colorectal cancers and may be associated with colorectal cancer development and progression.

Convenient Synthesis of N-Methylpyrrolidine-2-thione and Some Thioamides

Zong, Zhi-Min,Peng, Yao-Li,Liu, Zhi-Gang,Zhou, Shi-Lu,Wu, Lin,Wang, Xiao-Hua,Wei, Xian-Yong,Lee, Chul Wee 한국화학공학회 2003 Korean Journal of Chemical Engineering Vol.20 No.2

The synthesis of thioamides and thiolactams, which are used as important organic intermediates, has attracted great attention. However, expensive reagents, severe reaction conditions and low yields of the target products made conventional methods inconvenient and economically infeasible. To overcome these disadvantages, we investigated a new process for synthesizing thioamides and thiolactams. We examine thermal reactions of CS_2 with N-methyl-2-pyrrolidinones, formylamide, acetamide and N, N-dimethylformylamide, respectively. The results show that under optimum conditions N-methylpyttolidine-2-thione and the corresponding thioamides can be obtained in good to excellent yields by the above thionation reactions.

β-catenin protein utilized by Tumour necrosis factor-α in porcine preadipocytes to suppress differentiation

( Xiao Luo ),( Hui Xia Li ),( Rong Xin Liu ),( Zong Song Wu ),( Ying Juan Yang ),( Gong She Yang ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.6

Application of Organic Solvent System for Lipase-catalyzed Regioselective Benzoylation of 1-β-D-arabinofuranosylcytosine

Xiao-Feng Li,Min-Hua Zong,Guang-Lei Zhao,Yi-Gang Yu,Hui Wu 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.4

In this paper, enzymatic regioselective acylation of 1-β-D-arabinofuranosylcytosine (ara-C) with vinyl benzoate (VB) using immobilized Candida antarctica lipase B in binary organic solvents was explored. It was found that the lipase showed high regioselectivity (> 99%) towards the 5'-OH of ara-C in the representative organic solvent mixture (hexane-pyridine). To understand the enzymatic processes and provide a fair comparison of hexane-pyridine with C4MIm·PF6-pyridine (the representative ionic liquid-containing system), the effect of each process variable on the reactions in hexane-pyridine was investigated. The results indicate that the optimum hexane content,initial a w, molar ratio of VB to ara-C, and temperature were 28% (v/v), 0.11, 15, and 40°C, respectively. Under optimized conditions, the initial reaction rate in hexanepyridine (44.4 mM/h) was much higher than that in C4MIm·PF6-pyridine (29.4 mM/h) for each case. The maximum conversion yield, however, was increased when the reaction system was shifted from hexane-pyridine to C4MIm·PF6-pyridine. Further study revealed that the presence of an acidic by-product (benzoate acid, released during the acylation process) may cause rapid inactivation of the enzyme in hexane-pyridine, leading to a lower conversion rate, whereas the ionic liquid may have coating and protecting effects on the lipase during the reaction.

Monazites reveal timing and genesis of Nb-REE mineralization in trachyte from the Pingli area, North Daba Mountain, China

Xiao Nie,Zong-qi Wang,Lei Chen,Jingwu Yin,Gang Wang 한국지질과학협의회 2021 Geosciences Journal Vol.25 No.5

In recent years, several Nb-rare-earth-element (REE) deposits have been discovered in trachyte from the Pingli–Zhenping area of the North Daba Mountain. Of these, the Zhujiayuan Nb (-REE) deposit is particularly rich in monazite. In this study, electron probe microanalysis (EPMA) and laser-ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) were used to better understand the genesis of the deposit and constrain the timing of Nb-REE mineralization. The results show that most monazite in the deposit is closely related to various alteration minerals (biotite, sericite, albite, quartz, pyrite, and calcite), and monazite often has a lower ThO2 content (0.09–1.32 wt%), indicating its hydrothermal origin. Monazite and the dominant Nb-bearing mineral (columbite) have a close paragenetic relationship, indicating that Nb and REE were mineralized in the same period. LA-ICP-MS U-Pb dating on hydrothermal monazite grains shows a weighted average 206Pb/238U age of 237.2 ± 7.9 Ma, which represents the timing of REE mineralization. The origin of monazite, in combination with the alteration mineralogy and ore-forming age, suggests that fluid exsolved from the Early Mesozoic alkaline felsic melt induced widespread hydrothermal alteration and the formation of the Zhujiayuan Nb-REE deposit.

Inhibitory effects of osteoprotegerin on osteoclast formation and function under serum-free conditions

Ying-Xiao Fu,Jian-Hong Gu,Yi-Ran Zhang,Xi-Shuai Tong,Hong-Yan Zhao,Yan Yuan,Xue-Zhong Liu,Jian-Chun Bian,Zong-Ping Liu 대한수의학회 2013 Journal of Veterinary Science Vol.14 No.4

The purpose of this study was to determine whether osteoprotegerin (OPG) could affect osteoclat differentiation and activation under serum-free conditions. Both duck embryo bone marrow cells and RAW264.7 cells were incubated with macrophage colony stimulatory factor (M-CSF) and receptor activator for nuclear factor κB ligand (RANKL) in serum-free medium to promote osteoclastogenesis. During cultivation, 0,10, 20, 50, and 100 ng/mL OPG were added to various groups of cells. Osteoclast differentiation and activation were monitored via tartrate-resistant acid phosphatase (TRAP) staining,filamentous-actin rings analysis, and a bone resorption assay. Furthermore, the expression osteoclast-related genes, such as TRAP and receptor activator for nuclear factor κB (RANK),that was influenced by OPG in RAW264.7 cells was examined using real-time polymerase chain reaction. In summary,findings from the present study suggested that M-CSF with RANKL can promote osteoclast differentiation and activation,and enhance the expression of TRAP and RANK mRNA in osteoclasts. In contrast, OPG inhibited these activities under serum-free conditions.

Involvement of the Ca2+ signaling pathway in osteoprotegerin inhibition of osteoclast differentiation and maturation

Ying-Xiao Fu,Jian-Hong Gu,Yi Wang,Yan Yuan,Xue-Zhong Liu,Jian-Chun Bian,Zong-Ping Liu 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.2

The purpose of this study was to determine whether the Ca2+ signaling pathway is involved in the ability of osteoprotegerin (OPG) to inhibit osteoclast differentiation and maturation. RAW264.7 cells were incubated with macrophage colony-stimulating factor (M-CSF) + receptor activator of nuclear factor-κB ligand (RANKL) to stimulate osteoclastogenesis and then treated with different concentrations of OPG, an inhibitor of osteoclast differentiation. The intracellular Ca2+ concentration [Ca2+]i and phosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) in the different treatment groups were measured by flow cytometry and Western blotting, respectively. The results confirmed that M-CSF + RANKL significantly increased [Ca2+]i and CaMKII phosphorylation in osteoclasts (p < 0.01), and that these effects were subsequently decreased by OPG treatment. Exposure to specific inhibitors of the Ca2+ signaling pathway revealed that these changes varied between the different OPG treatment groups. Findings from the present study indicated that the Ca2+ signaling pathway is involved in both the regulation of osteoclastogenesis as well as inhibition of osteoclast differentiation and activation by OPG.

SOME RESULTS ON UNIQUENESS OF MEROMORPHIC SOLUTIONS OF DIFFERENCE EQUATIONS

Gao, Zong Sheng,Wang, Xiao Ming Korean Mathematical Society 2017 대한수학회논문집 Vol.32 No.4

In this paper, we investigate the transcendental meromorphic solutions with finite order of two different types of difference equations $${\sum\limits_{j=1}^{n}}a_jf(z+c_j)={\frac{P(z,f)}{Q(z,f)}}={\frac{{\sum_{k=0}^{p}}b_kf^k}{{\sum_{l=0}^{q}}d_lf^l}}$$ and $${\prod\limits_{j=1}^{n}}f(z+c_j)={\frac{P(z,f)}{Q(z,f)}={\frac{{\sum_{k=0}^{p}}b_kf^k}{{\sum_{l=0}^{q}}d_lf^l}}$$ that share three distinct values with another meromorphic function. Here $a_j$, $b_k$, $d_l$ are small functions of f and $a_j{\not{\equiv}}(j=1,2,{\ldots},n)$, $b_p{\not{\equiv}}0$, $d_q{\not{\equiv}}0$. $c_j{\neq}0$ are pairwise distinct constants. p, q, n are non-negative integers. P(z, f) and Q(z, f) are two mutually prime polynomials in f.

Expression of C4.4A is a Potential Independent Prognostic Factor for Patients with Gastric Cancer

Cheng, Da-Qing,Gu, Xiao-Dong,Li, Zhen-Yang,Xiang, Jian-Bin,Chen, Zong-You Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

C4.4A, a metastasis-associated gene, encodes a glycolipid-anchored membrane protein which is overexpressed in several human malignancies. However, there are few data available on C4.4A expression and its relationship with progression in gastric cancer. Our study was designed to explore the expression of C4.4A in gastric cancer and to correlate it with clinical outcome. C4.4A expression was studied by quantitative real-time RT-PCR and immunohistochemistry for assessment of correlations with clinicopathological factors. C4.4A mRNA expression was significantly up-regulated in gastric cancer as compared with noncancerous tissue (p<0.05)., being observed in 107 (88.4%) of the 121 gastric cancer cases by immunohistochemistry. We found that the expression of C4.4A mRNA was correlated with size of the tumor, depth of invasion, lymph node metastasis, distant metastasis and TNM stage. Moreover, patients with overexpression of C4.4A has a significantly worse survival (p<0.05). Further multivariable analysis indicated that the expression of C4.4A was an independent prognostic indicator for gastric cancer (p<0.05). In conclusion, overexpression of C4.4A correlates with metastatic potential of gastric cancer and C4.4A could be a novel independent prognostic marker for predicting outcome.

Serum adipokines play different roles in type I and II ketosis

Shen Liuhong,Zhu Yingkun,Xiao Jinbang,Qian Bolin,You Liuchao,Zhang Yue,Yu Shumin,Zong Xiaolan,Cao Suizhong 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.12

Objective: This study was conducted to investigate the differences in several serum adipokines in perinatal dairy cows with type I and II ketosis, and the correlations between these adipokines and the two types of ketosis. Methods: Serum adiponectin (ADP), leptin (LEP), resistin, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) levels, and energy balance indicators related to ketosis were measured. Type I and II ketosis were distinguished by serum glucose (Glu) and Y values and the correlations between adipokines in the two types of ketosis were analyzed. Results: β-Hydroxybutyric acid of type I ketosis cows was significantly negatively correlated with insulin (INS) and LEP and had a significant positive correlation with serum ADP. In type II ketosis cows, ADP and LEP were significantly negatively correlated, and INS and resistin were significantly positively correlated. Revised quantitative INS sensitivity check index (RQUICKI) values had a significantly positive correlation with ADP and had a very significant and significant negative correlation with resistin, TNF-α, and IL-6. ADP was significantly negatively correlated with resistin and TNF-α, LEP had a significantly positive correlation with TNF-α, and a significantly positive correlation was shown among resistin, IL-6, and TNF-α. There was also a significant positive correlation between IL-6 and TNF-α. Conclusion: INS, ADP, and LEP might exert biological influences to help the body recover from negative energy balance, whereas resistin, TNF-α, and IL-6 in type II ketosis cows exacerbated INS resistance and inhibited the production and secretion of ADP, weakened INS sensitivity, and liver protection function, and aggravated ketosis.