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Xiao-Ming Liu,Bang-Xian Zhang,Shi-Guang Li,Xiang-Jun Rao,Dong-MingWang,Xiao-Xuan Hu,Su Liu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2
The yellow mealworm beetle, Tenebrio molitor (Coleoptera: Tenebrionidae), is a common insect pest that damages stored grain. The insect olfactory co-receptor (Orco) is a transmembrane protein localized on the surface of the dendrites of olfactory sensory neurons and plays an essential role in the olfactory perception. In this study, an Orco ortholog (named as Tmol\Orco) inT.molitorwas characterized. The deduced amino acid sequence of Tmol\Orco shared significant high identities with Orcos in other insect species from distinct Orders. Reverse transcription quantitative PCR (RT-qPCR) analysis showed that Tmol\Orco was predominantly expressed in the antennae of both male and female adults, and the difference between male antennae and female antennae was not significant. Double-stranded RNA (dsRNA) directly against the Tmol\Orco mRNA was synthesized and injected into the T. molitor male adults, RT-qPCR results demonstrated that the transcription level of Tmol\Orco was significantly reduced at 6 d after the injection. The mate-seeking behavior of Tmol\Orco-silenced beetles was significantly inhibited. Compare to the control individuals, the percentage of Tmol\Orco-silenced beetles successfully find mateswas significantly decreased and the average time they spent for searching were significantly increased. These results provided first direct evidence that suppression of Tmol\Orco transcription contributes to the decline in mate recognition of T. molitor
Xiao-Xuan Zhang,Jing Jiang,Ya-Nan Cai,Chun-Feng Wang,Peng Xu,Gui-Lian Yang,Quan Zhao 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.1
A study of 426 rabbits from 3 cities in Jilin province (Changchun City and Jilin City) and Liaoning province (Shenyang City) was conducted between May and June 2015. The overall prevalence of E. bieneusi in rabbits was 0.94% (4/426), with 0% (0/116), 1.72% (3/174), and 0.74% (1/136) in Jilin, Changchun, and Shenyang City, respectively. Only 3 farms (farm 1 and farm 3 in Changchun City, farm 8 in Shenyang City) were PCR-positive for E. bieneusi. Moreover, rabbits of more than 6 months (1.72%) had the highest E. bieneusi prevalence, followed by rabbits of 4-6 months (1.26%), 2-3 months (0.58%), and less than 1 month (0%). Analysis of ITS gene of E. bieneusi suggested that all 4 E. bieneusi isolates were genotype D, and were classified as group 1a. The present results first demonstrated the existence of zoonotic E. bieneusi in domestic rabbits in China. Effective control measures should be implemented to prevent E. bieneusi infection in domestic rabbits, other animals, and humans.
Gene Flow from Transgenic Rice to Conventional Rice in China
( Xiao-xuan Du ),( Zhongze Piao ),( Kyung-min Kim ),( Gang-seob Lee ) 한국육종학회 2021 Plant Breeding and Biotechnology Vol.9 No.4
Global area of genetically modified crops (GM crops or biotech crops) continues to grow. It was 189.9 million hectares in 2017. Recently, a total of 24 countries have approved GM crops for planting and additional 43 countries have formally imported biotech crops for food, feed, and processing, meaning that biotech crops are now commonly accepted in those countries. With the continuous growth of the global population and the impact of climate change, research and commercialization of genetically modified crops are important for solving global food security issues in the future. At present, a large number of GM rice varieties have been cultivated in China (Chen et al. 2004; Jia 2004). Among them, GM rice varieties with insect resistance (Bt, CpTI genes), disease resistance (Xa21 genes), and herbicide resistance (bar, EPSPs genes) are waiting for relevant planting permits (Chen et al. 2004). In particular, two varieties, “Huahua 1” and “Shanyou 63”, developed by China Huazhong Agriculture Co., Ltd. have obtained GM rice safety certificate from the Ministry of Agriculture of China. However, there is still a lot of controversy in South Korea on the commercialization and safety research of GM products. This article aims to conduct a rational analysis of China's GM rice pollen mobility and China's current GM rice commercialization process to provide relevant reference basis for safety evaluation and future commercialization process of GM rice in South Korea.
Xuan, Zhu,Zhong, Zhao-Hui,Zhang, Xuan-Zhi,Zhang, Lei,Zhao, Xiao-Kun,Lv, Chen,Xu, Ran,Ren, Wei-Gang,Li, Song-Chao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3
To determine the relationship between comorbidity and outcome after radical cystectomy in Chinese patients by using the Adult Comorbidity Evaluation (ACE)-27 index. Two-hundred-and-forty-six patients treated with radical cystectomy at the Second Xiangya Hospital of Central South University, Hunan Province, China between 2000 and 2010 were retrospectively analyzed. Medical records were reviewed for age, gender, delayed time of radical cystectomy, urinary diversion type, pelvic lymphadenectomy status, TNM stage, and pathological grade. Comorbidity information was assessed by the ACE-27 index. The outcome measurement was overall survival. Univariate and multivariate Cox proportional hazards regression analyses were used to determine the association between comorbidity and outcome. The study population consisted of 215 (87.40%) males and 31 (12.60%) females with a mean age of $62{\pm}11$ years. Median duration of follow-up was $47{\pm}31$ months. A total of 151 (61.38%) patents died during follow-up. Of those, 118 (47.97%) had at least one comorbidity. According to the ACE-27 scores, 128 (52.03%) patients had no comorbidity, 79 (32.11%) had mild, 33 (13.41%) had moderate, and 6 (2.45%) had severe comorbidities. Multivariate analysis indicated that moderate (p=0.002) and severe (p<0.001) comorbidity was significantly associated with decreased overall survival. In addition, age ${\geq}70$ years (p=0.002), delayed time of radical cystectomy >12 weeks (p=0.044), pelvic lymphadenectomy status (p=0.014), and TNM stage >T3 (p<0.001) were determined to be independent risk factors of overall survival. Increasing severity of comorbidity statistically correlated with decreased overall survival after radical cystectomy.
Qing Xiao,Xuan Zhou,Zuobing Xiao,Yunwei Niu 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.2
The aroma composition, aroma-active compounds, and sensory attributions of cherry wines from three different price segments were investigated. A total of 48 aroma compounds were identified and quantified using headspace solid-phase microextraction (HS-SPME)/gas chromatography– mass spectrometry (GC–MS), among which 23 aroma compounds were further screened out as important odorants based on their odor activity values (OAVs); then, the previously mentioned 23 volatiles were correlated to sensory attributes using partial least-squares regression (PLSR). The result indicates that the differently priced wines were associated with different compounds and aroma attributions. Finally, aroma reconstitution was performed by mixing odor-active aroma compounds (OAVs>1) on the basis of their measured concentrations in the original sample. The result indicated that the aroma profile of the reconstituted sample was similar to that of the original sample.