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Xiao‑Xiao Gong,Bing‑Yu Yan,Jin Hu,Cui‑Ping Yang,Yi‑Jian Li,Jin‑Ping Liu,Wen‑Bin Liao 한국유전학회 2018 Genes & Genomics Vol.40 No.11
Tropical plant rubber tree (Hevea brasiliensis) is the sole source of commercial natural rubber and low-temperature stress is the most important limiting factor for its cultivation. To characterize the gene expression profiles of H. brasiliensis under the cold stress and discover the key cold stress-induced genes. Three cDNA libraries, CT (control), LT2 (cold treatment at 4 °C for 2 h) and LT24 (cold treatment at 4 °C for 24 h) were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. Quantitative real time PCR (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. A total of 1457 and 2328 differentially expressed genes (DEGs) in LT2 and LT24 compared with CT were respectively detected. Most significantly enriched KEGG pathways included flavonoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signal transduction, cutin, suberine and wax biosynthesis, Pentose and glucuronate interconversions, phenylalanine metabolism and starch and sucrose metabolism. A total of 239 transcription factors (TFs) were differentially expressed following 2 h or/and 24 h of cold treatment. Cold-response transcription factor families included ARR-B, B3, BES1, bHLH, C2H, CO-like, Dof, ERF, FAR1, G2-like, GRAS, GRF, HD-ZIP, HSF, LBD, MIKC-MADS, M-type MADS, MYB, MYB-related, NAC, RAV, SRS, TALE, TCP, Trihelix, WOX, WRKY, YABBY and ZF-HD. The genome-wide transcriptional response of rubber tree to the cold treatments were determined and a large number of DEGs were characterized including 239 transcription factors, providing important clues for further elucidation of the mechanisms of cold stress responses in rubber tree.
Xiao‐Juan Li,Guang-Ping DONG,Jian-Min FANG,Hong-Jian LIU,Wan-Lin GUO 한국곤충학회 2017 Entomological Research Vol.47 No.3
Monochamus alternatus is a destructive stem‐boring herbivore of Pinus massoniana, and the principal vector of pine wood nematode. To investigate the impacts of boring by M. alternatus larvae on the emission of volatile organic compounds (VOCs) from their host trees, the VOCs from uninfested and M. alternatus larvae infested P. massoniana trees were observed using a gas chromatograph–mass spectrometer. We detected 12, 9, 18 and 14 volatile organic compounds from infested xylem, infested phloem, uninfested xylem and uninfested phloem, respectively. In P. massoniana xylem, the boring of M. alternatus larvae induced cyclosativene, and inhibited 4‐carene, humulene, styrene, α‐phellandrene, β‐myrcene, β‐phellandrene and γ‐terpinene. The relative amounts of camphene, copacamphene, longicyclene, longifolene, tricyclene and α‐longipinene were significantly increased, and the relative amounts of α‐pinene and β‐pinene were significantly decreased by the boring behaviors of M. alternatus larvae. In P. massoniana phloem, the boring of M. alternatus larvae induced 2‐bornanone, copacamphene, longicyclene and α‐longipinene, and inhibited 2‐carene, 4‐carene, styrene, α‐phellandrene, β‐myrcene, β‐phellandrene, β‐pinene, γ‐terpinene and ο‐cymene. The relative amounts of camphene, caryophyllene and longifolene were significantly increased by the boring behaviors of M. alternatus larvae. The results indicate that the boring behaviors of M. alternatus larvae changed both the sorts and contents of the VOCs from P. massoniana trees.
Xiao‐Juan Li,Guang-Ping DONG,Jian-Min FANG,Hong-Jian LIU,Wan-Lin GUO 한국곤충학회 2018 Entomological Research Vol.48 No.4
The pine sawyer Monochamus alternatus Hope (Coleoptera: Cerambycidae) is a serious pest of several Pinus species, and the ectoparasitoid larvae of Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an important natural enemy of this pest. The transcriptome of M. alternatus larvae was sequenced using the Illumina platform and immunity‐related genes were specifically analyzed. De novo assembly resulted in the identification of 24 241 unigenes, with a mean length of 1122 bp, in unparasitized M. alternatus larvae and 23 807 unigenes, with a mean length of 1140 bp, for parasitized larvae. Removal of redundant unigenes resulted in 26 095 all‐unigenes, of which 16 959 (64.99%) showed clear homology with some of the known genes in the National Center for Biotechnology Information nr database. Parasitization had notable effects on the transcriptome profile of M. alternatus larvae. In all, 2702 genes were differentially expressed in M. alternatus larvae after parasitization, with 1491 (55.18%) upregulated and 1211 (44.82%) downregulated. Moreover, expression levels of immunity‐related genes in M. alternatus larvae were markedly altered in response to parasitization by D. helophoroides. In conclusion, the transcriptome profiling data, especially the discovered of immunity‐related genes, help illustrate the molecular mechanisms of parasitism between D. helophoroides and M. alternatus and provide new insights into developing immunity regulation‐mediated control methods of M. alternatus.
Improvement of CF/ABS Composite Properties by Anodic Oxidation of Pitch based C-type Carbon Fiber
Xiao Ping Yang,Cheng Zhong Wang,Yun Hua Yu,Seung-Kon Ryu 한국탄소학회 2002 Carbon Letters Vol.3 No.2
The surface treatment of C-type isotropic pitch-based carbon fiber was carried out by anodic oxidation in 5 wt% NH4NO3 electrolyte. The changes of fiber surface and carbon fiber/ABS resin composites were characterized by SEM, XPS and mechanical properties test. The oxygen functional groups on the surface, such as hydroxyl (-C-OH), carboxyl (-COOH) groups etc., increased after oxidation. Tensile strength, flexural strength and modulus of carbon fiber/ABS composites were also enhanced. However, the impact strength decreased with the improvement of the surface adhesion between CF and matrix.
Flexible multimode pressure sensor based on liquid metal
Xiao-Ping Zhou,Zihao Yu 국제구조공학회 2021 Smart Structures and Systems, An International Jou Vol.28 No.6
In this paper, a novel multimode liquid metal-based pressure sensor is developed. The main body of the sensor is composed of polydimethylsiloxane (PDMS) elastomer. The structure of the sensor looks like a sandwich, in which the upper structure contains a cylindrical cavity, and the bottom structure contains a spiral microchannel, and the middle partition layer separates the upper and the bottom structures. Then, the liquid metal is injected into the top cavity and the bottom microchannel. Based on linear elastic fracture mechanics, the deformation of the microchannel cross-section is theoretically analyzed. The changes of resistance, capacitance, and inductance of the microchannel under pressure are deduced, and the corresponding theoretical models are established. The theoretical values of the pressure sensor are in good agreement with experimental data, implying that the developed theoretical model can explain the performance of the sensor well.
Preparation of Homogeneous Microstructure Pure Lead Metaniobate by Two-Step Sintering
Xiao-gang Zhao,Peng Liu,Bo Chao,Su Juan Liu,Ming Pang,Xiao-Ming Chen,Jian-Ping Zhou 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.1
The purpose of the present work is to obtain the Lead metaniobate ceramics with the orthorhombic phase via a two-step sintering method. The samples were first sintered at 1320°C for 10 min, and then sintered separately at 1260°C, 1220°C, and 1180°C for 4 h. All the ceramics show orthorhombic phase and homogeneous microstructure. It was found that the abnormal grain growth was restrained obviously.
Xiao ping Jiang,Xiang Gao,Chao Shi 한국유체기계학회 2022 International journal of fluid machinery and syste Vol.15 No.2
Aiming at the problem that it is difficult to accurately predict the flexible operation conditions and state trend of hydraul ic turbine, in this paper a cascade model of BP LSTM classification prediction is proposed, which can identify the working conditions of existing fusion data, and then predict the measuring points of different working conditions. Based on the pressure parameters of hydraulic turbine units, t he improved BP neural network is used to determine the operation conditions of hydraulic turbine units, and the classified data is redivided to establish the multivariate LSTM prediction model. By optimizing the parameters of the multivariate LSTM predict i on model, such as the structure, the number of network layers and the number of hidden layer neurons, finally established the cascade model of BP LSTM classification prediction of time series of hydraulic turbine units. Through experimental verification a n d analysis, BP LSTM classification prediction model can predict the operation trend of measuring points under different working conditions after classification. Compared with other models, BP LSTM model has higher prediction accuracy and better effect. Th e cascade model of BP LSTM classification prediction of time series provides a model basis for the research of predictive control of hydraulic turbine units.
Position-dependent Spontaneous Motion of the Magnetic Domain Wall in Ferromagnetic Nanowires
Xiao-Ping Ma,Hong-Guang Piao,심제호,주하나 데데,김동현 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.2
We have investigated the dependence of the spontaneous dynamics of the magnetic domain wall in ferromagnetic nanowires on the initial position of the domain wall without any external field. Using micromagnetic simulations, we have observed in detail a spontaneous magnetic domain wall motion for various wire widths and thicknesses. We have found that the average speed of the spontaneous domain wall motion significantly depends on the wire width and thickness, as well as the starting position of the domain wall motion. We conclude that the spontaneous magnetic domain wall motion should be considered very carefully in practical design of spintronic devices based on magnetic domain wall motion.