http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Possible role of Pax-6 in promoting breast cancer cell proliferation and tumorigenesis
( Xiang Yun Zong ),( Hong Jian Yang ),( Yang Yu ),( De Hong Zou ),( Zhi Qiang Ling ),( Xiang Ming He ),( Xu Li Meng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.9
Pax 6, a member of the paired box (Pax) family, has been implicated in oncogenesis. However, its therapeutic potential has been never examined in breast cancer. To explore the role of Pax6 in breast cancer development, a lentivirus based short hairpin RNA (shRNA) delivery system was used to knockdown Pax6 expression in estrogen receptor (ER)-positive (MCF-7) and ER-negative (MDA-MB-231) breast cancer cells. Effect of Pax6 silencing on breast cancer cell proliferation and tumorigenesis was analyzed. Pax6-RNAi-lentivirus infection remarkably downregulated the expression levels of Pax6 mRNA and protein in MCF-7 and MDA-MB-231 cells. Accordingly, the cell viability, DNA synthesis, and colony formation were strongly suppressed, and the tumorigenesis in xenograft nude mice was significantly inhibited. Moreover, tumor cells were arrested at G0/G1 phase after Pax6 was knocked down. Pax6 facilitates important regulatory roles in breast cancer cell proliferation and tumor progression, and could serve as a diagnostic marker for clinical investigation. [BMB reports 2011; 44(9): 595-600]
Overexpression of ENA1 from Yeast Increases Salt Tolerance in Arabidopsis
( Xiang Qiang Kong ),( Xiu Hua Gao ),( Wei Huan Li ),( Ji Qiang Zhao ),( Yan Xiu Zhao ),( Hui Zhang ) 한국식물학회 2008 Journal of Plant Biology Vol.51 No.2
In yeast, the plasma membrane Na+/H+ antiporter and Na+ -ATPase are key enzymes for salt tolerance. Saccharomyces cerevisiae Na+ -ATPase (Ena1p ATPase) is encoded by the ENA1/PMR2A gene; expression of ENA1 is tightly regulated by Na+ and depends on ambient pH. Although Ena1p is active mainly at alkaline pH values in S. cerevisiae, no Na+ -ATPase has been found in flowering plants. To test whether this yeast enzyme would improve salt tolerance in plants, we introduced ENA1 into Arabidopsis (cv. Columbia) under the control of the cauliflower mosaic virus 35S promoter. Transformants were selected for their ability to grow on a medium containing kanamyin. Southern blot analyses confirmed that ENA1 was transferred into the Arabidopsis genome and northern blot analyses showed that ENA1 was expressed in the transformants. Several transgenic homozygous lines and wild-type (WT) plants were evaluated for salt tolerance. No obvious morphological or developmental differences existed between the transgenic and WT plants in the absence of stress. However, overexpression of ENA1 in Arabidopsis improved seed germination rates and salt tolerance in seedlings. Under saline conditions, transgenic plants accumulated a lower amount of Na+ than did the wild type, and fresh and dry weights of the former were higher. Other experiments revealed that expression of ENA1 promoted salt tolerance in transgenic Arabidopsis under both acidic and alkaline conditions.
Ren, Yong-Qiang,Han, Ju-Qiang,Cao, Jian-Biao,Li, Shao-Xiang,Fan, Gong-Ren Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
Objective: The objective of this study was to evaluate the association of MDR1 gene polymorphisms with susceptibility to hepatocellular carcinoma (HCC). Methods: A total of 689 HCC patients and 680 cancer-free subjects were enrolled. Human MDR1 gene polymorphisms were investigated by created restriction site-polymerase chain reaction (CRS-PCR) and DNA sequencing methods. Multiple logistic regression models were applied to estimate the association between MDR1 gene polymorphisms and susceptibility to HCC. Results: We detected a novel c.4125A>C polymorphism and our findings suggested that this variant was significantly associated with susceptibility to HCC. A significantly increased susceptibility to HCC was noted in the homozygote comparison (CC versus AA: OR=1.621, 95% CI 1.143-2.300, ${\chi}^2$=7.4095, P=0.0065), recessive model (CC versus AC+AA: OR=1.625, 95% CI 1.167-2.264, ${\chi}^2$=8.3544, P=0.0039) and allele contrast (C versus A: OR=1.185, 95% CI 1.011-1.389, ${\chi}^2$=4.4046, P=0.0358). However, no significant increase was observed in the heterozygote comparison (AC versus AA: OR=0.995, 95% CI 0.794-1.248, ${\chi}^2$=0.0017, P=0.9672) and dominant model (CC+AC versus AA: OR=1.106, 95% CI 0.894-1.369, ${\chi}^2$=0.8560, P=0.3549). Conclusions: These findings suggest that the c.4125A>C polymorphism of the MDR1 gene might contribute to susceptibility to HCC in the Chinese population. Further work will be necessary to clarify the relationship between the c.4125A>C polymorphism and susceptibility to HCC on larger populations of diverse ethnicity.
Qiang Yang,Xiang Li,Qingwen Tian,Aixiang Pan,Xingjian Liu,Hang Yin,Yingqiao Shi,Guigan Fang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-
Effective utilization solar energy through photocatalysis is an ideal way to solve environmental problemsand achieve sustainable development. Herein, a novel BiOBr/Lignin-Biochar photocatalyst has been successfullysynthesized by a simple hydrothermal method. The number of oxygen vacancies of BiOBrincreased after C doping, which improves visible-light absorbance, reduces the recombination ofphoto-generated carriers and promotes O2 activation to produce O2. UV–vis DRS result demonstratedthat the visible-light absorption capacity of BiOBr improved significantly with the addition of lignin. Compared with BiOBr, the adsorption and photocatalytic ability of BiOBr/Lignin-Biochar composites weregreatly enhanced due to enriched oxygen vacancies and the congenerous effect between BiOBr andlignin-biochar. The RhB removal with pure BiOBr and BiOBr/Lignin-Biochar under visible-light irradiationat 60 min was 54.5% and 99.2%, respectively, owing to the interface interaction between BiOBr and ligninbiocharpromoted the separation between electron and holes and the enrichment of RhB around the photocatalysts. Notably, the bandgap of BiOBr/Lignin-Biochar composites decreased from 2.65 eV to 2.56 eVafter C doping, useful for visible-light-driven photocatalysis. The superoxide radical anions (O2) were themain active species, as demonstrated by free radical capture experiments and ESR characterizationresults. Hence, the present work provides new insights into constructing cost-effective, high-efficiencycomposite materials for environmental remediation.
Composition and Antioxidant Activity of Water-Soluble Polysaccharides from Tuber indicum
Qiang Luo,Jie Zhang,Liang Yan,Yuanlin Tang,Xiang Ding,Zhirong Yang,Qun Sun 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.12
Crude water-soluble Chinese truffle Tuber indicum polysaccharide (TIP) was extracted from the fruiting bodies with water and then successively purified by DEAE–cellulose 52 and Sephadex G-100 column chromatography,yielding two major polysaccharide fractions: TIP1-1 and TIP2-1. High-performance gel permeation chromatography analysis showed that the average molecular sizes of TIP1-1 and TIP2-1 were approximately 1.75×10⁴Da and 5.73×10³ Da, respectively. Monosaccharide component analysis by gas chromatography indicated that TIP1-1 was composed of mannose,glucose, galactose, and rhamannose in the respective molar ratio of 3.93:1.24:0.75:1.26 and that TIP2-1 contained mannose,glucose, and arabinose in the respective molar ratio of 5.27:1.44:0.43. The antioxidant activity analyses revealed that TIP1-1and TIP2-1 possessed considerable antioxidant activity. Compared with TIP1-1, which has a higher molecular weight and contains no uronic acid, TIP2-1 exhibited a protective effect on PC12 cells injured by H₂O₂and a higher scavenging activity against free radicals. The relative effects of the lower molecular size, the presence of uronic acid, and the antioxidant activity of TIP2-1 appear to be significant. Accordingly, the Chinese truffle T. indicum might serve as an effective antioxidative healthcare food and source of natural antioxidants.
Event-triggered Control for Linear Systems with Model Uncertainty and Clock Offset
Xiang-hua Jiang,Qiang Ling 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.10
This paper investigates the input-to-state stability (ISS) of continuous-time networked control systems with model uncertainty and bounded noise based on event-triggering. The information generated at the sensor is quantized and transmitted to the controller through a digital communication channel, which suffers from the network-induced time delay and clock offset. In the concerned event-triggering framework, such the clock offset may cause long-term inconsistency between the state estimates of the sensor and the controller, and leads to the failure of ISS. By designing a well-designed state-dependent event-triggering threshold and updating methods, it can still ensure ISS for the concerned system in the presence of bounded clock offset and model uncertainty without exhibiting Zeno behavior. Simulations are done to verify the achieved results.
( Qiang Liu ),( Xuyu Xiang ),( Jiaohua Qin ),( Yun Tan ),( Qin Zhang ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.3
Traditional image steganography hides secret information by embedding, which inevitably leaves modification traces and is easy to be detected by steganography analysis tools. Since coverless steganography can effectively resist steganalysis, it has become a hotspot in information hiding research recently. Most coverless image steganography (CIS) methods are based on mapping rules, which not only exposes the vulnerability to geometric attacks, but also are less secure due to the revelation of mapping rules. To address the above issues, we introduced camouflage images for steganography instead of directly sending stego-image, which further improves the security performance and information hiding ability of steganography scheme. In particular, based on the different sub-features of stego-image and potential camouflage images, we try to find a larger similarity between them so as to achieve the reversible steganography. Specifically, based on the existing CIS mapping algorithm, we first can establish the correlation between stego-image and secret information and then transmit the camouflage images, which are obtained by reversible sub-feature retrieval algorithm. The received camouflage image can be used to reverse retrieve the stego-image in a public image database. Finally, we can use the same mapping rules to restore secret information. Extensive experimental results demonstrate the better robustness and security of the proposed approach in comparison to state-of-art CIS methods, especially in the robustness of geometric attacks.
Xiang-li Long,Zhi-hao Wang,San-qiang Wu,Shi-ming Wu,Hai-feng Lv,Wei-kang Yuan 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.1
Isophthalic acid (IPA) is commercially produced from m-xylene oxidation with the catalysis of thehomogeneous Co–Mn–Br catalyst system. In this study, a catalytic system consisting of HPW/C and Co(II)has been put forward to oxidize m-xylene (MX) to IPA. The experimental results prove that the HPW/Cand Co catalytic system is capable of catalyzing the oxidation of MX to IPA, which can obtain a higher MXconversion and IPA concentration than the homogeneous H3PW12O40/Co(OAc)2/Mn(OAc)2 catalyticsystem. The heterogeneous catalytic system is also advantageous over the homogeneous catalyticsystem in the inhibition of the oxidation of acetic acid and IPA. The optimal amount of phosphotungsticacid supported on carbon is 7.5% (wt). The best dosage of HPW/C is 15 g l-1. The optimum Co(II)concentration in the catalytic system for IPA production is 0.064% (wt). The best HPW/C activationtemperature is 220℃ .