Molecular characterization of chemosensory protein genes in Bactrocera minax (Diptera: Tephritidae)

Wang Zhao‐Xiang,Qi Zhen‐hua,Chen Jian,Wang Fu‐Lian,Gui Lian‐You,Zhang Guo‐Hui 한국곤충학회 2021 Entomological Research Vol.51 No.7

Chemoreception is of great importance for survival of insects. Chemosensory proteins (CSPs) are believed to be involved in the perireceptor events of chemosensory system in many insect species, but this has not been clarified in the citrus fruit fly, Bactrocera minax, a serious dipteran pest of citrus crops. Previous studies indicated that four CSP genes were identified in many fly speices in Diptera. In the present study, we also identified four CSP genes in B. minax,namely BminCSP1–4, from a transcriptome database. All CSP proteins encoded by these genes bear the typical hallmarks of the CSP family: an N-terminal signal peptide and the four highly conserved cysteine residues. Phylogenetic analysis comparing with other dipteran CSPs indicated that dipteran CSPs may evolved from three ancestral CSP genes, and revealed the sequence diversities of BminCSPs and showed that BminCSP1–4 are clustered in separate groups, indicating the possibility of their contrasting function in B. minax. Furthermore, the tissue distribution of the four CSP genes in adult B. minax was analyzed by real-time quantitiative PCR. The results demonstrated that BminCSP3 are significantly transcriptionally enriched in antennae; BminCSP4 expressed primarily in heads; BminCSP1 and BminCSP2 showed high expressions in different tissues, such as antennae, abdomens and wings. Based on these findings, the different implications for the functions of BminCSPs are discussed. This study will offer a significant indication for further functional studies of the CSPs in B. minax

할로아세틸시코닌 유도체의 합성 및 항암성 평가 : Synthesis and Evaluation of Antitumor Activity

鄭相國,金光洙,송규용,조훈,안병준 충남대학교 약학대학 의약품개발연구소 1998 藥學論文集 Vol.14 No.-

The secondary hydroxy group at side chain of shikonin structure was selectively acylated with various haloacetic acids in presence of dicyclohexylcarbodiimide and 4-dimethylaminopyridine to produce haloacetylshikonin derivatives. The cytotoxicity of monohaloacetylshikonin derivatives against L1210 cells increased in the following order: monochloroacetylshikonin (ED_50, 0.142 ㎍/㎖) nonobromoacetylshikonin (ED_50, 0.158㎍/㎖)>monoiodoacetylshikonin (ED_50, 0.173 ㎍/㎖). Introduction of larger halogen atoms decreased the cytotoxic activity, presumably due to steric hinderance. The cytotoxicity of chloroacetylshikonin derivatives was dependent on the number of chlorine atom, thus increasing in the following order : trichloroacetylshikonin (0.032 ㎍/㎖)>dichloroacetylshikonin (0.059 ㎍/㎖)> monochloroacetylshikonin (ED_50, 0.142 ㎍/㎖). Thus, the electron-withdrawing effect seems to be important for the cytotoxicity of chloracetylshikonin derivatives. Consistent with the above, dichloracetylshikonin (T/C, 182%) and trifluoroacetylshikonin (195%) showed higher T/C values than monochloroacetyl-(T/C, 122%), monobromoacetyl-(T/C, 154%) and monoiodoacetylshikonin (T/C, 117%) derivatives. Haloacetylshikonin derivatives showing lower cytotoxic activities against L1210 cells exhibited lower T/C values. It seems that there is a relationship between the cytotoxicity of haloacetylshikonin and their antitumor activity.

Structural Behavior Analysis of UHPC Hybrid Tower for 3-MW Super Tall Wind Turbine Under Rated Wind Load

Xiang-Guo Wu,Xuesen Zhang,Hom Bahadur Bhattarai,황현종,Jing Yang,강순필 한국콘크리트학회 2022 International Journal of Concrete Structures and M Vol.16 No.6

Based on the conceptual design of an advanced wind turbine tower system, use of ultra-high-performance cementitious composites material with compressive strength of 200 MPa (UHPC-200) is proposed to ensure high durability and ductility of the UHPC hybrid wind turbine tower. Key design parameters are proposed for the structural design of a 3-MW wind turbine. The material properties, mixing compositions, simplified constitutive relationship, and model parameters are outlined. Using nonlinear finite element analysis, the effects of wall thickness, wall thickness ratio, and prestressing tendon on the structural performance including the longitudinal stress field, lateral displacement, stress concentration at the transition zone between the middle and bottom segments are evaluated. Based on the stress-field analysis, the design limitation of the segmental wall thickness and its ratio is recommended. The numerical results show that the tower with the wall thickness ratio of 2:3 (i.e., thickness 200–300 mm) with prestressing tendons is an optimal design for the UHPC hybrid tower. The results of this study can be used as a reference for the engineering design of a new type of UHPC hybrid wind turbine tower.

Numerical simulation of heat transfer and fluid flow in laser+GMAW hybrid fillet welding of aluminum allow with a horizontal joint

Guo-Xiang Xu,Lin Li,Guo-Qing Hu 대한용접·접합학회 2018 대한용접학회 특별강연 및 학술발표대회 개요집 Vol.2018 No.11

Flexural behavior of precast concrete wall – steel shoe composite assemblies with dry connection

Xiang-Guo Wu,Xinlei Xia,Thomas H.-K. Kang,Jingcheng Han,김창수 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.29 No.4

This study aimed to investigate the flexural behavior of precast concrete (PC) wall – steel shoe composite assemblies with various dry connection details at mid-span. Flexural tests were performed for five scenarios. Test parameters included the width of test specimens, arrangement of steel shoe connectors, and use of structural adhesive or waterproof tape at the mid-span joint. The test results showed that the PC wall – steel shoe composite assemblies joined at mid-span showed flexural damage patterns combined with rotational deformation, and the structural performance was satisfactory regardless of the arrangement of steel shoe connectors. Considering the two deformation components (flexural deformation by bending and rotational deformation due to joint opening), a theoretical model was proposed to analyze flexural strength and joint opening, and the simple model gave good predictions with acceptable accuracy.

Experimental Study on Waterproofing Properties of Putty-Based Composite Rubber Strip for Underground Post-Tensioned Precast Concrete Structures

Xiang-guo Wu,Xiao-kai Chen,Shi-yan Yu,Seongwon Hong,Thomas H.-K. Kang 한국콘크리트학회 2019 International Journal of Concrete Structures and M Vol.13 No.1

The proper assembly of underground precast concrete structures is often critical in the construction of underground structures. In particular, interfacial waterproofing between precast concrete segments is a key factor influencing use, safety, and life span. Current practice is to incorporate waterproofing rubber strips in the design. During the installation process, compressive stress is applied to the strip by post-tensioning to achieve performance. For this paper, lateral constraint compression tests were carried out on composite rubber seal strips that utilize putty. Special waterproofing and sealing test devices were designed to investigate corresponding relationships between water pressure and compressive stress (or strain). A relationship between water resistance pressure and compression stress and strain of the putty-based composite rubber strip was proposed based on the series tests and the control target of the minimum compression strain of the putty composite rubber strip was then suggested. Finally, full-scale waterproofing tests on tunnel joints were conducted. The experimental results provide a scientific reference for the engineering application and design of composite sealing rubber strips putty for underground post-tensioned precast concrete structures.

Theoretical study of UHPCC composite column behaviors under axial compression

Xiang-Guo Wu,Ruofei Zou,Xinyu Zhao,Qun Yu 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.55 No.5

To improve the durability and service life of reinforced concrete column such as bridge piers, an advanced composite column made of Ultra High Performance Cementitious Composites (UHPCC) permanent form is proposed. Based on elasticity plasticity theory, axial compression behavior of the composite column was studied theoretically. The first circumferential cracking load and ultimate limit loading capacity are derived for the composite column. Short composite column compression tests and numerical simulations using FEM method were carried out to justify the theoretical formula. The effects of UHPCC tube thickness on the axial compression behavior were studied. Using the established theoretical model and numerical simulation, the large dimension composite columns are calculated and analyzed with different UHPCC tube thickness. These studies may provide a reference for advanced composite column design and application.

Flexural behavior of UHPC-RC composite beam

Xiang-Guo Wu,Yang Lin 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.22 No.2

In order to evaluate the effects of U shape ultra high performance concrete (UHPC) permanent form on the behaviors of Reinforced Concrete (RC) beam, a full scale RC composite beam is designed and tested with U shape UHPC permanent form and a reference RC beam with same parameters is tested simultaneously for comparison. The effects of the permanent form on the failure mode, cracking strength, ultimate capacity and deformation are studied. Test results shows that the contributions of the U shape UHPC permanent form to the flexural cracking behaviors of RC beam are significant. This study may provide a reference for the design of sustainable RC beam with high durable UHPC permanent form.

Acoustic localization of surface defect for low-speed large bearing with averaged generalized inverse beamforming

Xiang Guo,Xing Wu,Xiaoqin Liu,Linjiang Tang 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.1

Using microphone array to detect the fault of rotating machinery is an effective method. Compared with the fault diagnosis based on vibration analysis, the advantage of this method is that it does not need the direct contact between the sensor and the machine. In addition, acoustic location can be realized by using microphone array, to accurately identify the sound radiation source on the machine surface. Conventional beamforming (CBF) is widely applied in noise source identification because of its fast speed. In the local reverberation environment, the conventional beamforming is disturbed by the image source, causing inaccurate judgment of the fault location and low imaging resolution. In this paper, an acoustic imaging method of averaged generalized inverse beamforming (AGIB) is proposed. Compared with CBF, generalized inverse beamforming (GIB) possess higher location accuracy. First, the spectral kurtosis (SK) can enhance the impact characteristics of fault signals. According to the center frequency and bandwidth estimated by the spectral kurtosis diagram, the impact information which adopt in beamforming location can be extracted in time domain and frequency domain by spectral kurtosis matched filter. Then the average amplitude and standard deviation of generalized inverse beamforming output at different positions are calculated. Finally, the normalized standard deviation is used for the beamforming output weighting of the central measurement position, so as to enhance the spatially invariant source contribution and effectively attenuate the ghost caused by the local reverberation environment. Through defect location of low-speed large bearing, compared with the averaged conventional beamforming (ACBF), AGIB can not only overcome the influence of local reverberation environment and accurately locate the fault location, but also improve the resolution significantly.

제6회 한,중 형법 국제 학술심포지움 : 중국 미성년자범죄 형법규범 평술

손국상 ( Guo Xiang Sun ) 한국비교형사법학회 2008 비교형사법연구 Vol.10 No.2