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Yun-Xiang Zang,Doo-Hwan Kim,박범석,홍승범 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.4
Indole glucosinolates (IG), a group of secondary metabolites found almost extensively in the order Brassicales, play an important role in the interaction between plant and insect or microorganism. In order to explore the possibility of IG metabolic engineering in Chinese cabbage hairy roots, three Arabidopsis cDNAs CYP79B2, CYP79B3, and CYP83B1 combined with rolABC were introduced into Chinese cabbage by Agrobacterium-mediated transformation. Overexpression of CYP79B2, CYP79B3, or CYP83B1 alone did not affect the accumulation levels of IG in transgenic hairy roots. However, when CYP83B1 was overexpressed together with CYP79B2 and/or CYP79B3, some of the transgenic hairy roots accumulated higher levels of glucobrassicin (GBC) or 4-methoxy glucobrassicin (4-OMeGBC) than control hairy root line carrying rolABC vector. With regard to the IG accumulation, overexpression of all three cDNAs showed no better than overexpression of both cDNAs. Both 4-OMeGBC and neoglucobrassicin (neo-GBC) were found to be the main components of IG that comprise about 90% of total IG in all types of Chinese cabbage hairy root lines. In transgenic hairy root lines rB3B1-8 and rB2B1B3-5, 4-OMeGBC increased to 2 and 1.5-fold, while neo-GBC decreased to 0.5 and 0.6-fold, respectively. This suggests that an increased production of 4-OMeGBC causes a reduction of neo-GBC level since the two types derive from a common precursor GBC. However, in terms of the total IG level, the transgenic hairy roots did not show significant differences from controls.
Possible role of Pax-6 in promoting breast cancer cell proliferation and tumorigenesis
( Xiang Yun Zong ),( Hong Jian Yang ),( Yang Yu ),( De Hong Zou ),( Zhi Qiang Ling ),( Xiang Ming He ),( Xu Li Meng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.9
Pax 6, a member of the paired box (Pax) family, has been implicated in oncogenesis. However, its therapeutic potential has been never examined in breast cancer. To explore the role of Pax6 in breast cancer development, a lentivirus based short hairpin RNA (shRNA) delivery system was used to knockdown Pax6 expression in estrogen receptor (ER)-positive (MCF-7) and ER-negative (MDA-MB-231) breast cancer cells. Effect of Pax6 silencing on breast cancer cell proliferation and tumorigenesis was analyzed. Pax6-RNAi-lentivirus infection remarkably downregulated the expression levels of Pax6 mRNA and protein in MCF-7 and MDA-MB-231 cells. Accordingly, the cell viability, DNA synthesis, and colony formation were strongly suppressed, and the tumorigenesis in xenograft nude mice was significantly inhibited. Moreover, tumor cells were arrested at G0/G1 phase after Pax6 was knocked down. Pax6 facilitates important regulatory roles in breast cancer cell proliferation and tumor progression, and could serve as a diagnostic marker for clinical investigation. [BMB reports 2011; 44(9): 595-600]
Yun Xiang Zang,Seung Beom Hong,Dhandapani Murugesan,Jong Gu Lim,Doo Hwan Kim 한국원예학회 2006 Horticulture, Environment, and Biotechnology Vol.47 No.4
Two common bermudagrass (Cynodon dactylon L. Pers.) cultivars, ‘Sundevil Ⅱ’ and ‘Mirage’, were used to improve tissue culture responses in embryogenesis and regeneration. Embryogenic calli were induced from the seeds of the two cultivars. Callus started forming at the base of scutellum of germinating seeds about 7 days after culturing in callus induction medium. Callus induced on MS medium with 2 ㎎ㆍL?¹ -12,4-dichlorophenoxyacetic acid only was not regenerable. However, when 0.1 to 0.5 ㎎ㆍL?¹ 6-benzylaminopurine was added to the above medium, compact and yellowish callus formed and was regenerable. The induced embryogenic callus produced shoots about 5 days after transferring to regeneration medium with regeneration rates of more than 70% for the two cultivars. All of the regenerated plants rooted within 3 days and were morphologically normal after transferring to rooting medium.
Agrobacterium tumefaciens-mediated Hairy Root Production from Seedlings of Chinese Cabbage
( Yun Xiang Zang ),( Doo Hwan Kim ),( Seung Beom Hong ) 한국식물학회 2008 Journal of Plant Biology Vol.51 No.4
Cruciferous hairy roots are often used for improving drought adaptability, peroxidase production, and in vitro subculturing of Plasmodiophora brassicae. For metabolic engineering, Agrobacterium tumefaciens-mediated systems have previously been developed for hairy root production in other plant species. Here, we used the rolABC gene binary construct in A. tumefaciens strain GV3101 to establish cultures of Chinese cabbage hairy roots. On both solid and liquid media, the rolABC hairy root lines exhibited a wild-type hairy root syndrome in terms of their growth and morphology. This demonstrates that those three genes are sufficient to induce high-quality hairy roots in Chinese cabbage. Such a system could be useful for the stable production of secondary metabolites in that species.
( Xiang Yang Ge ),( Yan Xu ),( Xiang Chen ),( Long Yun Zhang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.1
This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermentation phase, and a low oxygen supply level in the later phase was carried out. As a consequence, a maximum L-LDH activity of 95.6 U/ml was obtained in the recombinant strain, which was over 4-fold higher than that of the initial strain. Under the TOS for L. casei (pMG-ldhL), the maximum lactic acid concentration of 159.6 g/l was obtained in 36 h, corresponding to a 62.8% increase. The results presented here provide a novel way to regulate the metabolic flux of L. casei for lactic acid production in different fermentation stages, which is available to enhance organic acid production in other strains.
La traduction du theatre francais en Chine
( Yun Zhang Xiang ) 한국통역번역학회 2007 FORUM Vol.5 No.2
This article first presents a list of French dramas which have been translated into Chinese and performed in China. By analysing the list of works, the author attempts to nail down the factors which influence both the translation and the performance of French dramas in China. In addition to the selection of dramas made by the translators or the stage directors from the cultural perspectives, there are still specific requirements for the translated works to be performed on the stage in China. This article highlights the importance for the translation works to recover the performance implicit in the text of the original works. Meanwhile, the author explores the process of drama translation by taking examples of the Chinese translation of Le Tartuffe, the famous play written by Moliere.
Fuzzy Ideals Extensions in Semigroups
XIANG YUN XIE 경북대학교 자연과학대학 수학과 2002 Kyungpook mathematical journal Vol.42 No.1
In this paper, we shall introduce the notions of fuzzy ideal extension property, strongly fuzzy extension property and fuzzy property of a semigroup S. We also discuss the relations of these fuzzy ideal extension poperties and some properties of semigroups with the fuzzy ideal extension prorerty. Furthermore, we prove that a semigroup has the ideal extension property if and only if S has the strongly fuzzy ideal extension property. As an application of the results of this paper, we obtain characterizations of cyclic semigroups