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Xi-Jun Yin,Hyo-Sang Lee,양철주,배인휴,오동환,Seong-Gyun Cho,공일근 아세아·태평양축산학회 2007 Animal Bioscience Vol.20 No.5
This study was carried out to evaluate the effect of artificial insemination (AI) time and methods on fertilization and delivery rate at onset of estrus cats. Artificial insemination method was used by a transcervical insemination (TCI) or intra-vaginal insemination (IVI) using frozen epididymal sperm (FES) with Norwegian catheter. Semen was collected from epididymal spermatozoa after testis ectomization and frozen in Tris-buffered solution supplemented with 7% glycerol and 0.5% Orvus ES Paste. The concentration of frozen spermatozoa was adjusted to 200106 sperm/ml. The CASA data on motility and progressive motility of FES after thawing was approximately 40.35.8 and 35.96.5%. The female cats were subcutaneously treated with 50 IU pregnant mare serum gonadotropin (PMSG) and ovulated with 100 IU human chorionic gonadotropin (hCG) 4 days after PMSG injection. One treatment was inseminated at 36 h after hCG injection and the other treatment was inseminated immediately hCG injection. At 36 h after hCG injection group, 5 female cats were inseminated TCI or IVI method using 50106 sperm/0.2 ml per AI. Three of 5 female cats could be inseminated by TCI method and then delivered the 10 kittens from 2 of 3 inseminated females. Immediately hCG injection group, 4 female cats were inseminated TCI or IVI method using same sperm concentration per AI. Two of 4 female cats could be inseminated by TCI method and then delivered the 4 kittens from 1 of 2 inseminated female cats. The results revealed that AI time and method could be affected the fertilization and delivery rates at onset of estrus cats.
Growth Response of Hot Pepper Applicated with Ammonium (NH₄⁺) and Potassium (K⁺)-Loaded Zeolite
Jun-Xi Li(이준시),Chi-Do Wee(위치도),Bo-Kyoon Sohn(손보균) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.5
고추 멀칭재배에서 암모늄이온 (NH₄?)과 칼륨이온 (K?)을 흡착시킨 천연 Zeolite (NK-Z) 처리 효과를 확인하기 위하여, 관행기비, 관행기비와 NK-Z 추비 및 NK-Z 기 ? 추비를 처리하고 고추 유묘를 이식한 후 고추의 생육반응과 토양 중 질소와 칼륨농도의 변화를 경시적으로 조사하였다. 이식 18주 후 NK-Z 기 ? 추비 처리구의 고추는 대조구보다 생육이 크게 향상되었다. 고추과실이 익은 이후 4차례에 걸쳐 수확을 하여 적과의 수량을 비교한 결과, NK-Z 기?추비 처리구의 주당 생체중으로 대조구보다 14.89% 증가하였다. 고추재배 토양 중 NH₄?과 K?의 농도의 변화를 분석한 결과 NK-Z 기 ? 추비, NK-Z 추비 및 대조구의 순으로 질소와 칼륨농도가 높게 유지되었으며, 이러한 결과는 암모늄이온(NNH₄?)과 칼륨이온(K?)이 흡착된 천연 Zeolite가 토양 중 보비력을 증가시킨다. NK-Z 기 ? 추비 처리구의 NH₄-N 농도가 대조구보다 63.41% 높았다. The feasibility of using ammonium (NH₄?) and potassium (K?)-loaded zeolite (NK-Z) as a slow-release fertilizer to control nitrogen and potassium supply was investigated in this study. The growth responses, which were determined in terms of shoot length, shoot fresh weight, and fresh fruit weight, were greater in plants treated with NK-Z than in those treated with chemical fertilizers (CF) after 18 weeks of transplantation. The total fruit weight per plant in treated with NK-Z as the basal and additional fertilizer (ZBAF) was 14.89% higher than that of CF. The nitrogen and potassium contents in NK-Z amended soils were higher than those in CF amended soils in the final stage of plant growth. The ammonium nitrogen (NH₄-N) concentration in ZBAF amended soils was 63.41% higher than that in CF amended soils.
( Jun Pei Zhou ),( Qian Wu ),( Rui Zhang ),( Yu Ying Yang ),( Xiang Hua Tang ),( Jun Jun Li ),( Jun Mei Ding ),( Yan Yan Dong ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6
This paper reports the production and characterization of crude xylanase from the newly isolated Humicola sp. Ly01. The highest (41.8 U/ml) production of the crude xylanase was obtained under the optimized conditions (w/v): 0.5% wheat bran, 0.2% KH2PO4, and 0.5% peptone; initial pH 7.0; incubation time 72 h; 30℃; and 150 rpm. A considerable amount of the crude xylanase was induced using hulless barley bran or soybean meal as the carbon source, but a small amount of the enzyme was produced when supplementary urea was used as the nitrogen source to wheat bran. The crude xylanase showed apparent optimal cellulase-free xylanase activity at 60℃ and pH 6.0, more than 71.8% of the maximum xylanase activity in 3.0-30.0% (v/v) ethanol and more than 82.3% of the initial xylanase activity after incubation in 3.0-30.0% (v/v) ethanol at 30℃ for 2 h. The crude xylanase was moderately resistant to both acid and neutral protease digestion, and released 7.9 and 10.9 μmol/ml reducing sugar from xylan in the simulated gastric and intestinal fluids, respectively. The xylooligosaccharides were the main products of the hydrolysis of xylan by the crude xylanase. These properties suggested the potential of the crude enzyme for being applied in the animal feed industry, xylooligosaccharides production, and high-alcohol conditions such as ethanol production and brewing.
( Jun Mei Ding ),( Ting Ting Yu ),( Lian Ming Liang ),( Zhen Rong Xie ),( Yun Juan Yang ),( Jun Pei Zhou ),( Bo Xu ),( Jun Jun Li ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11
The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around 50°C at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an α/β-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.
Improved photocatalytic performance of Pd-doped ZnO
Jun bo Zhong,Jian zhang Li,Xi yang He,Jun Zeng,Yan Lu,Wei Hu,Kun Lin 한국물리학회 2012 Current Applied Physics Vol.12 No.3
ZnO and Pd-doped ZnO photocatalysts with different molar ratio of Pd/Zn (1/100, 2/100, 3/100 and 4/100) were prepared by a solegel method. The photocatalysts prepared were characterized by BET surface area, X-ray diffraction (XRD), UV/vis diffuse reflectance (DRS) and surface photovoltage spectroscopy (SPS), respectively. The results show that doping Pd into ZnO decreases the BET surface area. The XRD spectra of the Pd-doped ZnO catalysts calcined at 773 K show only the characteristic peaks of wurtzite-type. Doping Pd into ZnO increases the absorbance of ZnO in visible region and enhances the photoinduced charge separation rate. The photocatalytic activity of Pd-doped ZnO photocatalysts for decolorization of methyl orange (MO) solution was evaluated, of all the photocatalysts prepared, the Pddoped ZnO with 3/100 possesses the best photocatalytic activity. The results of further experiments show that increased adsorption ability of light and high separation rate of photoinduced charge carriers all play an important role in promotion of photocatalytic activity of Pd-doped ZnO nanostructure.
Molecular and Biochemical Characterization of a Novel Intracellular Low-Temperature-Active Xylanase
( Jun Pei Zhou,),( Yan Yan Dong ),( Xiang Hua Tang ),( Jun Jun Li ),( Bo Xu ),( Qian Wu ),( Ya Jie Gao ),( Lu Pan ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.4
A 990 bp full-length gene (xynAHJ2) encoding a 329- residue polypeptide (XynAHJ2) with a calculated mass of 38.4 kDa was cloned from Bacillus sp. HJ2 harbored in a saline soil. XynAHJ2 showed no signal peptide, distinct amino acid stretches of glycoside hydrolase (GH) family 10 intracellular endoxylanases, and the highest amino acid sequence identity of 65.3% with the identified GH 10 intracellular mesophilic endoxylanase iM-KRICT PX1-Ps from Paenibacillus sp. HPL-001 (ACJ06666). The recombinant enzyme (rXynAHJ2) was expressed in Escherichia coli and displayed the typical characteristics of low-temperatureactive enzyme (exhibiting optimum activity at 35 o C, 62% at 20 o C, and 38% at 10 o C; thermolability at ≥45 o C). Compared with the reported GH 10 low-temperature-active endoxylanases, which are all extracellular, rXynAHJ2 showed low amino acid sequence identities (<45%), low homology (different phylogenetic cluster), and difference of structure (decreased amount of total accessible surface area and exposed nonpolar accessible surface area). Compared with the reported GH 10 intracellular endoxylanases, which are all mesophilic and thermophilic, rXynAHJ2 has decreased numbers of arginine residues and salt bridges, and showed resistance to Ni 2+ , Ca 2+ , or EDTA at 10 mM final concentration. The above mechanism of structural adaptation for low-temperature activity of intracellular endoxylanase rXynAHJ2 is different from that of GH 10 extracellular low-temperature-active endoxylanases. This is the first report of the molecular and biochemical characterizations of a novel intracellular low-temperatureactive xylanase.