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Xian-Yong Lan,Chu-Zhao Lei,Yong-Tao Huai,Chuan-Ying Pan,Jing Wang,Yong-Zhen Huang,Hong Chen 한국유전학회 2011 Genes & Genomics Vol.33 No.6
Sine oculis homeobox homolog 3 (Six3) gene is responsible for normal mammalian pituitary development, and its genetic variations or deficiency will cause hypopituitarism, suggesting that this gene is a potential candidate gene for studying association with growth traits in animals. Herein, genetic variants within bovine Six3 gene was screened in 1031 individuals from four Chinese indigenous cattle breeds. Two novel polymorphisms (NC_007309:g.2515G>A and NC_007309:g.2607T>C) locating at positions nt1707 and nt1799 of intron 1 in bovine Six3gene, were found, and could be genotyped by TaqI ACRS PCR-RFLP and Alw26I PCR-RFLP, respectively. The frequencies of allele “A” of TaqI locus varied from 0.004 to 0.309,as well as the frequencies of allele “C” of Alw26I locus waved from 0.025 to 0.340. Association analysis revealed no significant association of TaqI locus with growth traits in Nanyang breed. However, significant relationships between Alw26I locus and body weight (BW) and average daily gain (ADG) in Nanyang breed was found (p<0.05). The individuals with genotype TC had greater body weight and average daily gain than those with genotype TT at 18 months old. Furthermore, based on combinated genotypes from these two loci, diplotypes was found to be associated with growth traits (p<0.05).The individuals with dihaplotype GG-TC had greater body weight and average daily gain at 18 month-old than those of other dihaplotypes. Therefore, the TaqI and Alw26I genetic variants of bovine Six3 gene were recommended as DNA markers related to growth traits through marker-assisted selection for genetics and breeding in cattle.
( Yong Tae Jeong ),( Yong Deuk Kim ),( Young Mi Jung ),( Dong Chan Park ),( Dong Sub Lee ),( Sae Kwang Ku ),( Xian Li ),( Yue Lu ),( Guang Hsuan Chao ),( Keuk Jun Kim ),( Jai Youl Lee ),( Moon Chang B 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
Low molecular weight fucoidan (LMWF) is widely used to treat metabolic disorders, but its physiologic effects have not been well determined. In the present study, we investigated the metabolic effects of LMWF in obese diabetic mice (leptin receptor-deficient db/db mice) and the underlying molecularmechanisms involved in endoplasmic reticulum (ER) stress-responsive L6 myotubes. The effect of LMWF-mediated AMP-activated protein kinase (AMPK) activation on insulin resistance via regulation of the ER stress-dependent pathway was examined in vitro and in vivo. In db/db mice, LMWF markedly reduced serum glucose, triglyceride, cholesterol, and low-density lipoprotein levels, and gradually reduced body weights by reducing lipid parameters. Furthermore, it effectively ameliorated glucose homeostasis by elevating glucose tolerance. In addition, the phosphorylation levels of AMPK and Akt were markedly reduced by ER stressor, and subsequently, glucose uptake and fatty acid oxidation were also reduced. However, these adverse effects of ER stress were significantly ameliorated by LMWF. Finally, in L6 myotubes, LMWF markedly reduced the ER stress-induced upregulation of the mammalian target of rapamycin-p70S61 kinase network and subsequently improved the action of insulin via AMPK stimulation. Our findings suggest that AMPK activation by LMWF could prevent metabolic diseases by controlling the ER stress-dependent pathway and that this beneficial effect of LMWF provides a potential therapeutic strategy for ameliorating ER stress-mediated metabolic dysfunctions.
Analysis of a mode-I crack perpendicular to an imperfect interface
Zhong, Xian-Ci,Li, Xian-Fang,Lee, Kang Yong Elsevier 2009 International journal of solids and structures Vol.46 No.6
<P><B>Abstract</B></P><P>The elastostatic problem of a mode-I crack embedded in a bimaterial with an imperfect interface is investigated. The crack is in proximity to and perpendicular to the imperfect interface, which is governed by linear spring-like relations. The Fourier transform is applied to reduce the associated mixed-boundary value problem to a singular integral equation with Cauchy kernel. By numerically solving the resulting equation, stress intensity factors near both crack tips are evaluated. Obtained results reveal that the stress intensity factors in the presence of the imperfect interface vary between that with a perfect interface and that with a completely debonding interface. Moreover, an increase in the interface parameters decreases the stress intensity factors. In particular, when crack approaches to the weakened interface closer, the stress intensity factors become larger for a sliding interface, and become larger or smaller for a Winkler interface, depending on the crack lying in a stiffer or softer material. The influences of the imperfection of the interface on the stress intensity factors for a bimaterial composed of aluminum and steel are presented graphically.</P>
Cat fertilization by mouse sperm injection
Jin, Yong-Xun,Cui, Xiang-Shun,Yu, Xian-Feng,Lee, Sung-Hyun,Wang, Qing-Ling,Gao, Wei-Wei,Xu, Yong-Nan,Sun, Shao-Chen,Kong, IL-Keun,Kim, Nam-Hyung Cambridge University Press 2012 Zygote Vol.20 No.4
<B>Summary</B><P>Interspecies intracytoplasmic sperm injection has been carried out to understand species-specific differences in oocyte environments and sperm components during fertilization. While sperm aster organization during cat fertilization requires a paternally derived centriole, mouse and hamster fertilization occur within the maternal centrosomal components. To address the questions of where sperm aster assembly occurs and whether complete fertilization is achieved in cat oocytes by interspecies sperm, we studied the fertilization processes of cat oocytes following the injection of cat, mouse, or hamster sperm. Male and female pronuclear formations were not different in the cat oocytes at 6 h following cat, mouse or hamster sperm injection. Microtubule asters were seen in all oocytes following intracytoplasmic injection of cat, mouse or hamster sperm. Immunocytochemical staining with a histone H3-m2K9 antibody revealed that mouse sperm chromatin is incorporated normally with cat egg chromatin, and that the cat eggs fertilized with mouse sperm enter metaphase and become normal 2-cell stage embryos. These results suggest that sperm aster formation is maternally dependent, and that fertilization processes and cleavage occur in a non-species specific manner in cat oocytes.</P>
플라스틱 기판에 펜타센 유기박막트랜지스터를 이용한 집적회로 제작
허영헌(Yong-Xian Xu),황성범(Sung-Beom Hwang),송정근(Chung-Kun Song) 대한전자공학회 2007 電子工學會論文誌-SD (Semiconductor and devices) Vol.44 No.11
본 연구에서는 하부전극 구조 펜타센 유기박막트랜지스터를 이용하여 플라스틱 기판에 인버터, 링 발진기, NAND & NOR 논리게이트, 정류기 등 간단한 집적회로를 제작하고 그 특성을 관찰하였다. 제작된 유기박막트랜지스터 소자의 평균 전하이동도는 0.26 ㎠/V.sec, 전류점멸비는 10?로 나타났으며 인버터와 NAND, NOR 논리게이트는 입력에 대해 정확한 논리출력값을 출력하였다. 전파 정류기는 1㎒의 AC 입력신호에 대해 정류효과를 나타냈으며 링 발진기는 DC 40V에서 1㎒의 발진특성을 보였다. 이와 같이 유기박막트랜지스터를 이용한 집적회로를 제작하고 특성을 분석함으로써 현재 관심이 되고 있는 초저가 RFID tag, Flexible Display 구동회로 등에 유기박막트랜지스터를 적용할 수 있는 가능성을 확인하였다. In this article, the organic integrated circuits such as inverter, ring oscillator, NAND and NOR gates, and rectifier have been fabricated on PEN substrate by using pentacene TFTs, The OTFTs used bottom contact structure and produced the average mobility of 0.26 ㎠/V.sec and on/off current ratio of 10?. All circuits successfully worked as the simulation results. Especially, the rectifier was able to operate up to 1 ㎒ input AC signals, and ring oscillator exhibited oscillation frequency of 1㎒ at 40 V. Based on the results of organic integrated circuits we could confirm the possibility of the low cost RFID tags and flexible display with OTFTs.
( Eun Kyung Yoon ),( Yong Tae Jeong ),( Xian Li ),( Song Cui ),( Dong Chan Park ),( Yong Hoon Kim ),( Yong Deuk Kim ),( Hyeun Wook Chang ),( Sang Han Lee ),( Seung Lark Hwang ) 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
Glyceollin has been shown to have antidiabetic properties, although its molecular mechanism is not known. Here, we have investigated the metabolic effects of glyceollin in animal models of insulinresistance and in endoplasmic reticulum (ER) stress-responsive muscle cells. db/db mice were treated with glyceollin for 4weeks and triglycerides, total cholesterol, low-density lipoprotein (LDL) and high-density lipoprotein (HDL) levels were measured. Glyceollin reduced serum insulin and triglycerides and increased HDL levels in db/db mice. Furthermore, glyceollin caused a significant improvement in glucose homeostasis without altering body weight and food intake in db/db mice. In muscle cells,glyceollin increased the activity of AMP-activated protein kinase (AMPK) as well as cellular glucose uptake. Fatty acid oxidation was also increased. In parallel, phosphorylation of acetyl-CoA carboxylase (ACC) at Ser-79 was increased, consistent with decreased ACC activity. An insulin-resistant state was induced by exposing cells to 5μg/ml of tunicamycin as indicated by decreasedinsulin-mediated tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1) and glucose uptake. Inhibition of insulin-mediated tyrosine phosphorylation of IRS-1 and glucose uptake under ER stress was prevented by glyceollin. Strikingly, glyceollin reduced ER stress-induced, c-Jun NH2-terminal kinase activation and subsequently increased insulin signaling via stimulation of AMPK activity in L6 myotubes. Pharmacologic inhibition or knockdown of Ca(2+)/calmodulin-dependent protein kinase kinase blocked glyceollin-increased AMPK phosphorylation and insulin sensitivity under ER stress conditions. Taken together, these results indicate that glyceollin-mediated enhancement of insulinsensitivity under ER stress conditions is predominantly accomplished by activating AMPK, thereby having beneficial effects on hyperglycemia and insulin resistanced.