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Jeong, Wooseog,Yoon, Hachung,Kim, Yong Kwan,Moon, Oun-kyong,Kim, Do-Soon,An, Dong-Jun Wildlife Disease Association 2014 JOURNAL OF WILDLIFE DISEASES Vol.50 No.4
<P>Abstract Toxoplasma gondii is an obligate intracellular protozoan parasite and a commonly encountered pathogen in humans and animals. The wild boar (Sus scrofa coreanus) is considered a good indicator when monitoring environmental contamination by T. gondii. We surveyed the prevalence of antibodies against T. gondii in wild boars from South Korea. Blood samples were collected from 426 wild boars captured in eight provinces of South Korea during the hunting seasons in 2008-12. Antibodies against T. gondii were detected using an indirect enzyme-linked immunosorbent assay in samples from 152 of boars, indicating an overall antibody prevalence of 36% (95% confidence interval=31-40%).</P>
Estimation of prevalence and sampling to estimate prevalence of Bovine Tuberculosis
( Hachung Yoon ),( Wooseog Jeong ),( Jae Woon Jeong ),( Youn Ju Kim ),( Woo Jin Jeon ),( Oun Kyong Moon ) 한국예방수의학회(구 한국수의공중보건학회) 2013 예방수의학회지 Vol.37 No.1
This study describes a series of processes for development of a survey aimed at understanding the actual state of an infectious disease. It includes of a survey for estimation of prevalence, analysis of investigated data and appraisal of the operation. Examples were inspired from a survey on bovine tuberculosis in Korean native cattle (Bos Taurus coreanae), conducted between October and December 2007. The methodology concerns stratification, two-stage cluster sampling, intraduster correlation, and the probability of detecting cases in a population. This study is expected to serve as an example in operating surveillance and monitoring systems for animal health programs at the national level.
A HPLC-UV method for quantification of ivermectin in solution from veterinary drug products
Kim, Young-Wook,Jeong, Wooseog The Korean Society of Veterinary Service 2022 韓國家畜衛生學會誌 Vol.45 No.3
The HPLC conditions for analysis of ivermectin in solutions dosage forms of commercial anthelmintics are different for each product. The purpose of this study was to establish a standardized chromatographic method for the quantification of ivermectin in solution. The separation was achieved on Waters Xbridge C18 column (4.6×150 nm, 5 ㎛) using different kinds of mobile phase composed of water/methanol/acetonitrile (15/34/51, v/v and 19.5/27.5/53, v/v), with UV detection at wavelengths 245 nm and 254 nm. A total of five commercial ivermectin in solution samples were analyzed. In this study, the optimal chromatographic conditions for analysis of ivermectin in solution were mobile phase of water/methanol/acetonitrile (15/34/51, v/v) at a flow rate of 1.0 mL/min and a detection wavelength of 245 nm using a Waters Xbridge C18 column (4.6×250 nm, 5 ㎛) at a column temperature of 25℃. The linearity was observed in the concentration range of 50~150 ㎍/mL, with a correlation coefficient, r<sup>2</sup>= 0.99999. The limit of detection and the limit of quantification were 0.88 and 2.68 ㎍/mL, respectively. The accuracy (% recovery) was found to be 98.9 to 100.3%. Intra-day and Intermediate precisions with relative standard deviations were less than 1.0%. The content of ivermectin for five market samples ranged 91.2~102.7%. The proposed method was also found to be robust, therefore, the method can be used for the routine analysis of ivermectin in solutions dosage forms.
Phylogenetic analysis of feline panleukopenia virus (FPLV) strains in Korean cats
An, Dong-Jun,Jeong, Wooseog,Jeoung, Hye-Young,Yoon, Sook Hee,Kim, Hyun-Jeong,Park, Jee-Yong,Park, Bong-Kyun Elsevier 2011 Research in veterinary science Vol.90 No.1
<P><B>Abstract</B></P><P>Sixteen Korean feline panleukopenia virus (FPLV) strains were compared with 48 non-Korean strains and two vaccine strains to conduct phylogenetic analysis of the FPLVs currently circulating among cats in Korea. Most of the residues that discriminate between FPLVs and canine parvoviruses (CPV-2, -2a, -2b, and -2c), including 80-Lys, 93-Lys, 103-Val, 323-Asp, 564-Asn, and 568-Ala, were conserved in the Korean FLPVs; however, exceptions were observed in two strains, namely K50/08 (80-Gln) and V142 (323-Asn). Phylogenetic analysis using the Bayesian inference and Neighbor-joining method showed that FPLVs were not segregated on a clear temporal or geographical basis. Three clusters (G1, G2, and G3) were formed by the VP2 nucleotide sequences analysed and Korean strains belonged to the G1 (<I>n</I>=13) and G2 (<I>n</I>=3) clusters. The ratio of non-synonymous to synonymous substitutions (dN/dS) revealed that purifying selection acts on the VP2 gene of Korean FPLVs.</P>
Seong In Lim,Wooseog Jeong,Dong Seob Tark,Dong Kun Yang,권창희 대한수의학회 2009 Journal of Veterinary Science Vol.10 No.4
Bovine leukemia virus (BLV) envelope glycoprotein (gp51/ gp30T-), consisting of BLV gp51 and BLV gp30 that lacked its C-terminal transmembrane domain, was expressed in insect cells under the control of the baculovirus polyhedron promoter. Recombinant BLV gp51/gp30T- secreted from insect cells was determined by immunofluorescence, enzymelinked immunosorbent and western blot assays using a BLV-specific monoclonal antibody and BLV-positive bovine antibodies. An agar gel immunodiffusion (AGID) test using gp51/gp30T- as the antigen for the detection of BLV antibodies in serum was developed and compared to traditional AGID, which uses wild type BLV antigen derived from fetal lamb kidney cells. AGID with the recombinant BLV gp51/gp30T- was relatively more sensitive than traditional AGID. When the two methods were tested with bovine sera from the field, the recombinant BLV gp51/gp30T- and traditional antigen had a relative sensitivity of 69.8% and 67.4%, respectively, and a relative specificity of 93.3% and 92.3%. These results indicated that the recombinant BLV gp51/gp30T- is an effective alternative antigen for the diagnosis of BLV infection in cattle.
Conditions for the disinfectant efficacy test under subzero temperatures
Chae, Won-Seok,Jeong, Wooseog,Lee, Hu-Jang The Korean Society of Veterinary Science 2019 大韓獸醫學會誌 Vol.59 No.1
To establish appropriate conditions for a disinfectant efficacy test at subzero temperatures, this study examined mixtures of frozen foot-and-mouth disease virus or avian influenza virus solutions and disinfectant diluents at $-5^{\circ}C$ and monitored temperature and freezing status of an anti-freezing diluent (AFD, 15% ethanol + 30% propylene glycol + 55% distilled water) over time at various subzero temperatures. Viral solutions and disinfectant diluents froze before the mixtures reached $-5^{\circ}C$, whereas the AFD was not frozen at $-30^{\circ}C$. The times taken for the AFD to reach -10, -20, -30, and $-40^{\circ}C$ from room temperature were 36, 39, 45, and 48 min, respectively.