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( Woori Na ),( Jiyu Kim ),( Hyeji Kim ),( Yeji Lee ),( Bonghee Jeong ),( Sung Pyo Lee ),( Cheongmin Sohn ) 한국임상영양학회 2021 Clinical Nutrition Research Vol.10 No.1
Adequate nutritional intake in elderly individuals improves frailty. Elderly individuals may exhibit improvements in frailty with the use of community care facilities. Therefore, this study evaluated the effects of nutritional intervention in elderly subjects at community care facilities receiving oral nutritional supplements (ONSs) and determined their nutritional status. Sixty-two elderly individuals using community care facilities were divided into the experimental group (EG) (before [n = 31]/after [n = 28]) and control group (CG) (before [n = 31]/after [n = 25]). Subjects in both groups were treated with ONSs (200 mL/200 kcal) for 90 days. However, those in the EG received the product with increased protein; vitamins A, C, D, and E; phosphorus; calcium; and zinc. The data collected included anthropometric data, dietary assessment findings, frailty status (Korean version of the Fatigue, Resistance, Ambulation, Illnesses, and Loss of weight questionnaire), and nutritional status (Mini Nutritional Assessment, MNA). The changes in the two groups were analyzed using the Mann-Whitney U Wilcoxon signed-rank test. Nutritional intervention increased the weight, body mass index, and lean body mass in the EG (p < 0.05). Protein, calcium, and iron levels increased only in the EG (p < 0.05). The MNA score increased and sum of frailty indicators improved in the EG, and the increase in the MNA score in the EG was greater than that in the CG. This study verified the improved anthropometric data and dietary intake in the EG. Thus, the higher number of pre-frailty elderly individuals at facilities of community care indicates the need for adequate nutritional supplementation for frailty management.
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The recent application of next generation sequencing to developmental biology
Woori Kwak 한국발생생물학회 2014 한국발생생물학회 학술발표대회 Vol.2014 No.9
The advent of Next Generation Sequencing (NGS) technology has changed the research paradigm and become an essential tool for recent biological and medical study. In today’s market, there are several sequencing platforms which have specific sequencing principle, and the result of each sequencing platform has different characteristics among them. Hence, each sequencing method became more specialized for specific research purpose, and researchers who consider NGS analysis have to understand the very basic characteristics of each NGS platform. NGS is used in various studies and they are usually classified into 5 categories (Re-sequencing, RNA-seq, de novo assembly, Metagenomics and Epigenomics) of analysis. In this session, we will introduce the characteristics of sequencing platforms and examples of recent research on each of the 5 analysis categories. In addition, we will talk about the benefit of NGS study compared to the traditional study and how these NGS technologies can be applied in developmental biology research.
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( Woori Na ),( Bonghee Chung ),( Cheongmin Sohn ) 한국임상영양학회 2019 Clinical Nutrition Research Vol.8 No.3
An increase in the prevalence of dyslipidemia has been strongly associated with the mortality rate of cardiovascular disease. We conducted a cross-sectional analysis to determine the relationship between dietary patterns and dyslipidemia in adult men aged 40-64 years. A total of 5,643 subjects from the Korean Genome and Epidemiology Study (KoGES) were selected for the final analysis. We analyzed 24-hour dietary recall data. Using principal component analysis, 3 dietary patterns were identified: rice based Korean food pattern, flour based western dietary pattern, and convenience food dietary pattern. The flour based western dietary pattern was significantly and positively associated with total cholesterol, and low density lipoprotein (LDL) cholesterol (p for trend < 0.05). With regard to the rice based Korean food pattern, the group with the highest factor score had a significantly lower risk of hyper LDL cholesterolemia (odds ratio [OR], 0.802; 95% confidence interval [CI], 0.698-0.922) and high total cholesterol levels (OR, 0.868; 95% CI, 0.761-0.990) than the group with the lowest factor score. For the flour based western dietary pattern the group with the highest factor score showed a significantly high risk of hyper LDL cholesterolemia (OR, 1.189; 95% CI, 1.033-1.367; p for trend < 0.05) than the group with the lowest factor score. Our results showed that the rice based Korean food pattern protected against dyslipidemia. In contrast, the western staple pattern, which is rich in flour and processed foods, was independently associated with dyslipidemia in urban male residents of Korea.
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Diastolic dysfunction and acute kidney injury in elderly patients with femoral neck fracture
( Woori Cho ),( Tae Yeon Hwang ),( Yoon Kyung Choi ),( Ji Hyun Yang ),( Myung-gyu Kim ),( Sang-kyung Jo ),( Won Yong Cho ),( Se Won Oh ) 대한신장학회 2019 Kidney Research and Clinical Practice Vol.38 No.1
Background: Femoral neck fracture is common in the elderly population. Acute kidney injury (AKI) is an important risk factor for mortality in patients who have had such fracture. We evaluated the incidence of AKI in patients who had femoral neck fracture and identified risk factors for AKI and mortality. Methods: This was an observational cohort study including 285 patients who were ≥ 65 years of age and who underwent femoral neck fracture surgery between 2013 and 2017. Results: The mean age was 78.63 ± 6.75 years. A total of 67 (23.5%) patients developed AKI during the hospital stay: 57 (85.1%), 5 (7.5%), and 5 (7.5%) patients were classified as having stage 1, 2, and 3 AKI, respectively. Patients with AKI had a lower baseline estimated glomerular filtration rate and higher left atrial dimension, left ventricular mass index, pulmonary artery pressure, and the ratio of early mitral inflow velocity to early diastolic mitral annulus velocity (E/e’) and were more likely to have diabetes or hypertension (HTN) (P < 0.05). The presence of HTN (odds ratio [OR], 4.570; 95% confidence interval [CI], 1.632-12.797) higher E/e’ (OR, 1.105; 95% CI, 1.019-1.198), and lower hemoglobin (OR, 0.704; 95% CI, 0.528-0.938) were independently associated with a higher risk for developing AKI. Severe AKI (OR, 24.743; 95% CI, 2.822-212.401) was associated with a higher risk of mortality. Conclusion: Elderly patients with femoral neck fracture had a high incidence of AKI. Diastolic dysfunction was associated with AKI. Severe AKI was associated with in-hospital mortality.
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Woori KIM,Soonkyu HWANG,Neung-Ho AHN,Byoung-Hee LEE,Byung-Kwan CHO 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Blowfly and flesh fly have specific ecological characteristics that their larvae grow in decaying environments. To survive in this habitat against harmful pathogens and bacteria, the fly species produced antimicrobial peptides (AMPs), which are considered promising candidates for treating bacterial infections. In this study, to discover such valuable novel AMPs, three fly species were isolated from Pyeongchang-gun, Gangwon-do, South Korea. Through analysis of the mitochondrial cytochrome oxidase I gene, they were identified as Calliphora vicina, Lucilia illustris (Calliphoridae), and Sarcophaga kanoi (Sarcophagidae). Despite their medicinal and veterinary importance, the genetic information of these species has not been fully examined. Therefore, we generated transcriptome data sets of larval salivary glands and performed de novo transcriptome assembly. As a result, 29,531, 42,167, and 31,862 unigenes were assembled. These transcriptome data of blowfly and flesh fly isolated in Korea will allow the discovery of useful AMPs.
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Woori Na,Jiyu Kim,Bong Hee Chung,Dai-Ja Jang,Cheongmin Sohn 한국영양학회 2020 Nutrition Research and Practice Vol.14 No.4
BACKGROUND/OBJECTIVES: Given the increasing proportion of the Korean population that is aged 65 years and older, the present study analyzed the relationship between diet quality and sarcopenia in elderly persons by using data from the 2008–2011 Korea National Health and Nutrition Examination Survey (KNHANES). SUBJECTS/METHODS: Data for 3,373 persons aged 65 years and over (men: 1,455, 43.1%) were selected from the 2008–2011 KNHANES. Sarcopenia assessments are based on a formula that divides a subject"s appendicular skeletal muscle mass (ASM) by their weight (wt) and multiplies that result by 100 ([ASM/wt] × 100). Sarcopenia is present if the subject"s result was less than one standard deviation (SD) below the sex-specific mean for a young reference group. For evaluation of diet quality, data obtained via the 24-hour recall method were used to calculate the Diet Quality Index for Koreans (DQI-K). A general linear model was applied in order to analyze general information and nutritional intake according to sarcopenia status. For analysis of the relationship between diet quality and sarcopenia, a binominal logistic regression analysis was undertaken. RESULTS: The sarcopenia prevalence rate among the study subjects aged 65 years and over was 37.6%. The DQI-K of those without sarcopenia was 3.33 ± 0.04 points, while that of those with sarcopenia was 3.45 ± 0.04 points (P < 0.05). The relationship between diet quality and sarcopenia revealed that subjects aged 75 and older had a poor diet quality, and their odds ratio (OR) of sarcopenia presence was significantly higher (OR: 1.807, 95% confidence interval: 1.003–3.254, P < 0.05). CONCLUSIONS: This study revealed that poor diet quality was related to sarcopenia presence in Koreans aged 75 and older. In order to improve the diet quality of the elderly (aged 75 and older), it is necessary to develop dietary improvement guidelines.
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Applicability of Soil Suitability Class Assessed with the Quantification Theory I
Woori Go,Byunghwan Seo,Songrae Cho,Yeonkyu Sonn 한국토양비료학회 2022 한국토양비료학회지 Vol.55 No.2
This study was conducted to suggest a method to assess soil suitability for crops objectively with categorical soil data and multivariate analysis methods. The Quantification Theory I was selected for the multivariate analysis method and the comprehensive point method was used as the conventional analysis tool for soil suitability assessment. The sites to survey soil characteristics and fig production were determined to include all classes of soil samples for all soil factors. Soil factors were soil texture of the subsoil, drainage class, available soil depth, gravel contents, and soil slope. In order to apply it nationwide based on the materials of the survey area, we used the 1,361 soil symbols data in Korea. After calculating the quantity forecast value for each soil factors, it was divided into four equal parts in descending order of very suitable, suitable, possible, and not suitable area. In the comprehensive point method, points were applied for each soil factor, and then classified according to the sum of the scores. The result of comparing the soil suitability class of the two setting methods the 1,361 soil symbols, 876 (64.4%) had the same grade, and 485 (35.6%) were classified as one grade lower or higher. Quantification Theory I is considered to have the potential to be utilized because it has the advantage of being able to classify at once through statistical analysis without subjective intervention if there is crop quantity data.
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