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Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions
Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8
Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.
Sokho Kim,Myung-Hoon Oh,Bum-Seok Kim,Won-Il Kim,Ho-Seong Cho,Byoung-Yong Park,Chul Park,Gee-Wook Shin,Jungkee Kwon 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.4
Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been suffi- ciently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to 200mM) for 1 h before treatment with 1 mg/ mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.
Kim, Sokho,Oh, Myung-Hoon,Kim, Bum-Seok,Kim, Won-Il,Cho, Ho-Seong,Park, Byoung-Yong,Park, Chul,Shin, Gee-Wook,Kwon, Jungkee The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.4
Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been sufficiently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to $200{\mu}M$) for 1 h before treatment with 1 mg/mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.
Protective Effects of Hyperoside against Carbon Tetrachloride-Induced Liver Damage in Mice
Choi, Jun-Ho,Kim, Dong-Wook,Yun, Nari,Choi, Jae-Sue,Islam, Md. Nurul,Kim, Yeong-Shik,Lee, Sun-Mee American Chemical Society and American Society of 2011 Journal of natural products Vol.74 No.5
<P>In this study, the hepatoprotective effects of hyperoside (<B>1</B>), a flavonoid glycoside isolated from <I>Artemisia capillaris</I>, have been examined against carbon tetrachloride (CCl<SUB>4</SUB>)-induced liver injury. Mice were treated intraperitoneally with vehicle or <B>1</B> (50, 100, and 200 mg·kg<SUP>−1</SUP>) 30 min before and 2 h after CCl<SUB>4</SUB> (20 μL·kg<SUP>−1</SUP>) injection. Levels of serum aminotransferases were increased 24 h after CCl<SUB>4</SUB> injection, and these increases were attenuated by <B>1</B>. Histological analysis showed that <B>1</B> prevented portal inflammation, centrizonal necrosis, and Kupffer cell hyperplasia. Lipid peroxidation was increased and hepatic glutathione content was decreased significantly after CCl<SUB>4</SUB> treatment, and these changes were reduced by administration of <B>1</B>. Protein and mRNA expression of tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and heme oxygenase-1 (HO-1) and nuclear protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2) significantly increased after CCl<SUB>4</SUB> injection. Compound <B>1</B> suppressed TNF-α, iNOS, and COX-2 protein and mRNA expression and augmented HO-1 protein and mRNA expression and Nrf2 nuclear protein expression. These results suggest that <B>1</B> has protective effects against CCl<SUB>4</SUB>-induced acute liver injury, and this protection is likely due to enhancement of the antioxidative defense system and suppression of the inflammatory response.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2011/jnprdf.2011.74.issue-5/np200001x/production/images/medium/np-2011-00001x_0006.gif'></P>
Azithromycin으로 치료한 임신 중 쓰쓰가무시병 1예
김광석,최진욱,서호종,김기훈,박성호,서광섭,고성만,김순혜,김호정 대한감염학회 2001 감염 Vol.33 No.5
We report a case of tsutsugamushi disease in a 26 year-old pregnant woman who was treated with azithromycin. Her gestation period was 27 weeks and she admitted with fever, rash, and eschar on the right shoulder. Currently recommended medications for the treatment of scrub typhus are doxycycline or chloramphenicol. But, these drugs are class D drugs according to the U.S. Food and Drug Administration (FDA) Fetal Risk Summary, so they couldn't be used to treat pregnant women. Recently, a few case reports suggested that azithromycin, a relatively new macrolide antibiotic, was effective and safe for the treatment of scrub typhus in pregnant women. And, there is no evidence that azithromycin causes harmful effects to the developing fetus or to children. On the basis of current in vivo test that confirms the effectiveness of azithromycin, it may be the drug of choice for the treatment of scrub typhus in pregnant women and children. (Korean J Infect Dis 33:380-382, 2001)
신증후군, 단클론성 감마병증 및 다발성 골용해 병변으로 발현한 소형 림프구성 림프종 1예
유준호,안명주,김호중,이웅수,박용욱,박문향 한양대학교 의과대학 1999 한양의대 학술지 Vol.19 No.2
We reported here a 70-year-old woman with small lymphocytic lymphoma who was initially presented with recurrent nephrotic syndrome and later developed multiple bone lytic lesions and monoclonal gammopathy, mimicking plasma cell dyscrasia and only involving bone marrow without lymphadenopathy or hepatosplenomegaly. She responded to combination chemotherapy, including cyclophosphamide and prednisone with proteinuria disappearing. The unusual features of this case includes : (1) nephrotic syndrome of minimal change lesion as the initial manifestion in lymphocytic lymphoma, (2) only bone marrow involvement of lymphoma without organomegaly or lymphadenopathy, (3) the presence of multiple bone lytic lesions in conjunction with monoclonal gammopathy in a patient with lymphoma rather than multiple myeloma.
활성탄관에 포집된 2MEA와 2EEA의 에스테르화가 2MEA와 2EEA 분석에 미치는 영향
김현욱,김강윤,최호춘 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.1
This study was performed to find an appropriate desorbing solution for 2MEA and 2EEA collected activated charcoal. The desorbing solution should be transesterification-free and have reliable desorption efficiency. Anumber of desorbing solutions were tested against CS2 and MeOH/ methylene chloride(5/95, v/v, 5MeOH) which were normally used as desorbing solutions. Transesterfication of 2MEA and 2EEA on dry charcoal by 5MeOH was also verified. A GC/MSD was used for verification of transesterification. Desorption effciencies of several desorbing solutions were measured by GC/FID, As desorbing solutions, CS2, 5MeOH, DMF/CS2(1/99, v/v, 1DMF), and DMF/cs2(3/97,v/v, 3DMF)were selected. Desorption effciencies of 2MEA and 2EEA were 97.3% and 99.2% by 5MeOH, respectively, and 100.8% and 101.4% by 3DMF, respectively. No significant difference between 5MeOH and 3DMF was found for both 2MEA and 2EEA(p〈0.05), Transesterification of 2MEA and 2EEA on dry charcoal at≥10 ppm was confirmed when 5MeOH was used as the desorbing solution, However, when 3DMF was used, no transestserification of 2MEA and 2MEEA on dry charcoal was found. This study found that 3DMF (DMF/CS@, 3/97, v/v)was the desorbing solvent of choice for 2MEA and 2EEA collected on coconut shell charcoal tube With the solutiom, no transesterification was found with nearly 100% desorption efficiency.
韓國 섬 集團에 있어서 노랑 초파리(Drosophila melanogaster)의 P-M System에 의한 Cytotype 決定 및 알코올탈수소효소(ADH) 活性度 分析
金彧,馬皓銓 단국대학교 1992 論文集 Vol.26 No.-
Cytotype distributions from three island populations(Cheje isl., Yeongjong isl., Ulnung isl.) of D. melanogaster in Korea were examined. It was also conducted to compare the differences for the ADH activity and lethal gene frequencies among strain types. P activities of three island populations were found to be very low. There were mainly M' and Q cytotypes in three island populations. These results suggested that the cytotype polymorphism could be maintained in those populations. The frequencies and patterns of cytotype distribution were very similar to the results of other reports. It might be interpreted as the results of migration between these islands and mainland or some forms of natural selection. The differences of ADH activity among strain types were found to be not significant, though the activity of M' strain was somewhat higher than that of Q strain. The frequencies of recessive lethal genes appeared to be nearly same among strain types, though lethal gene frequency of M' strain was slightly higher than that of Q strain.
人工海水에 의한 複合組織鋼의 表面腐蝕이 疲勞破壞强度에 미치는 影響
吳世旭,姜鎬珉,李悅宰 東亞大學校 大學院 1986 大學院論文集 Vol.11 No.-
The influence of seawater corrosion on fatigue life at low stress level appears to be larger than that at high stress level about M.E.F. dual phase steel. Especially, the variation-range between high stress level and low stress level is large in the sea bottom corrosion of 250 hours and 1000 hours about S-N curves. According to corrosion time and corrosion conditon the amount of reduction of fatigue limit and fatigue life is varied. Fatigue limit and fatigue life for sea surface corrosion condition is lower than those for sea bottom corrosion condition and the influence of sea surface corrosion of fatigue life is large. It is appeared the relation between fatigue length and the number of cycle is depended on stress level, corrosion time and corrosion condition and corrosion pits occurred by seawater corrosion are become the cause of micro-crack. The variation-range of the crack propagation rate for low stress level is larger than that for high stress level. Portions of these fracture surface don't occur plastic deformation by S.E.M observation. However, these are found quasi-cleavage fracture that occur seperate fracture.