Tracking the transition behavior and dynamics of ionic transport in crystalline ionic gel electrolytes

Park, HoSeok,Kwon, Se Ra,Jung, Young Mee,Kim, Hoon Sik,Lee, Hyun Joo,Hong, Won Hi Royal Society of Chemistry 2009 Chemical communications Vol.2009 No.42

<P>The transition behavior and dynamics of ionic transport were strongly influenced by changes in the crystal structure and interaction field of the crystalline ionic gel electrolytes with respect to chemical compositions, as proven by impedance, <SUP>7</SUP>Li NMR, PCA and 2D IR COS.</P> <P>Graphic Abstract</P><P>The dynamics and transition behavior of ion transport in binary crystalline ionic gel electrolytes consisting of 1-methylimidazolium-3-propanesulfonate (ImiZIL) and lithium bis(trifluorosulfonyl) amide (LiTFSI) were analyzed by impedance, <SUP>7</SUP>Li NMR, PCA and 2D IR COS. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b910600f'> </P>

Genotypic Variation of Helicobacter pylori Isolated from Gastric Antrum and Body in Korean Patients

Park, Seon-Mee,Kwon, Soon-Kil,Son, Bo-Ra,Shin, Kyeong-Seob,Woo, Chan-Won,Kim, Eung-Gook,Kim, Seok-Yong The Korea Society for Microbiology 2000 大韓微生物學會誌 Vol.35 No.1

Although most persons infected with Helicobacter pylori harbor a single strain of the organism, multiple strain colonization in the same patient is also occasionally reported in developed countries. The aims of this study were to determine the prevalence of multiple strain colonization in Korean patients and to detect the cagA, iceA1, and babA status of H. pylori isolated from the antrum and body of the stomach. H. pylori was obtained from 35 patients from the antrum and body of the stomach. The genomic diversity of H. pylori was determined by random amplified polymorphic DNA analysis. The status of cagA, iceA1, and babA genes of H. pylori was assessed by polymerase chain reaction with appropriate primers. Clearly different diversity patterns were identified among the isolates from 35 individual patients. Eighteen (51.4%) patients had a single strain of H. pylori. Eight (22.9%) and nine (25.7%) patients had subtypically (one or two bands difference) and typically (clearly different pattern) different strains of H. pylori in the antrum and body, respectively. Among the 70 isolates of H. pylori from 35 patients, the positive rates of 349-bp and 208-bp cagA gene fragments and the iceA1 gene were 68/70 (97.1%), 68/70 (97.1%), and 58/70 (82.9%), respectively. However, the babA gene was found in 22/66 cases (31.4%). In five out of 18 patients with a single strain, the genetic status of cagA, iceA1, and babA varied between the isolates from the antrum and the body. In 8/17 patients with sub typically or typically different strains, the gene status differed between antrum and body isolates. The prevalence of co-colonization with typically or subtypically different strains is high in Korea, and sub-clones with different pathogenic gene status exist within strains of identical RAPD patterns.

Antioxidant and prooxidant properties of ascorbic acid on hepatic dysfunction induced by cold ischemia/reperfusion

Park, Sang-Won,Lee, Sun-Mee Elsevier 2008 european journal of pharmacology Vol.580 No.3

<P><B>Abstract</B></P><P>Oxidative stress, which has been generated during reperfusion after a liver transplant, has been implicated in the higher rates of postoperative organ dysfunction. The aim of this study was to examine the effect of ascorbic acid on reperfusion injury after hepatic cold preservation. Isolated perfused rat livers were preserved in a University of Wisconsin solution for 30?h at 4?°C. The bile output was significantly lower after cold ischemia/reperfusion. In contrast, the portal pressure, lactate dehydrogenase and purine nucleoside phosphorylase activities were elevated by cold ischemia/reperfusion. These changes were attenuated at ascorbic acid concentrations of 0.25 and 0.5?mM. However, they were augmented at a concentration of 2?mM. Cold ischemia/reperfusion decreased the reduced to oxidized glutathione ratio, whereas it increased the level of lipid peroxidation and mitochondrial swelling. These changes were prevented exposing the liver to 0.5?mM ascorbic acid but were augmented at 2?mM ascorbic acid. These results suggest that cold ischemia/reperfusion injury is associated with a higher level of oxidative stress and ascorbic acid may act not only as an antioxidant but also as a prooxidant during cold ischemia/reperfusion.</P>

Breeding biology of Crow tit Paradoxornis webbiana fulvicauda (Campbell) in Korea

Park, Eun-Mee,Kim, Chang-Hoe,Yamagishi, Satoshi,Won, Pyoung-Oh 경희대학교 한국조류연구소 1993 연구보고 Vol.4 No.-

본 논문은 1989년부터 1990년까지 경기도 남양주군 동국대학교 운길산 연습림에서 붉은머리오목눈이 Paradoxornis webbiana fulvicauda(Campbell)의 번식생태에 관하여 조사한 것이다. 1. 붉은머리오목눈이는 암수공동으로 둥지를 틀며, 14종의 수종에 26개의 둥지를 틀었다. 둥지는 타원형이었으며 17개 둥지의 평균 측정치는 외경 69.63±4.67(장경, 평균±SD)㎜×62.76±5.78(단경, 평균±SD)㎜, 내경 56.44±6.26×49.245±5.24㎜ 이었고, 둥지의 깊이는 4.5±0.64㎝(평균±SD), 지상으로부터의 높이는 65.58±22.93㎝이었다. 2. 알은 순백색과 청록색의 두가지 색깔형태가 있으며, 청록색 알이 순백색의 알보다 컸다. 20개 알의 평균 크기는 장경 16.88±0.54㎜, 단경 12.82±0.06㎜, 무게는 1.34±0.06g 이었다. 3. 포란은 13~14일 동안 암수공동으로 하였고, 수컷이 암컷보다 긴 시간 포란하였다. 4. 성조의 평균측정치는 부척 20.01±1.24㎜(n=49), 날개 51.01±2.17㎜(n=50), 꼬리 67.54±3.82㎜(n=50) 및 체중은 10.85±0.93g(n=49)이었다. 5. 붉은머리오목눈이의 한배산란수는 4~6개였고, 평균 5.31(n=26)이었다. 6. 평균부화율은 66.85%이었고, 전체번식성공율은 43.23%였다. 7. 새끼의 성장속도계수(K)는 0.382였으며, 최대체중의 점근치(asymptote)는 10.0g, t_(50)은 4.11일 이었으며, 육추기간은 8-9일, 이소시의 체중은 평균 8.5g 이었다. 8. 붉은머리오목눈이의 음성은 3가지 형태의 울음소리(call)와 노래소리 (song)로 구별되었다.

(-)-Pinoresinol Monomethyl Ether Inhibits LPS-Induced iNOS and COX-2 Expression via the Attenuation of NF-${\kappa}B$ in Raw 264.7 Macrophage Cells

Park, Bo-Young,Lee, Mee-Young,Ahn, Kyung-Seop,Chin, Young-Won,Lee, Hyeong-Kyu,Oh, Sei-Ryang The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2

Anti-inflammatory effects of (-)-pinoresinol monomethyl ether (PME) were evaluated on lipopolysaccharide (LPS)-treated Raw 264.7 macrophage cells. PME inhibited translocation of p65-nuclear factor-${\kappa}B$ (NF-${\kappa}B$) into the nucleus in immunocytochemical analysis for NF-${\kappa}B$ activation, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in Western blotting, as well as production of pro-inflammatory mediators, such as nitric oxide (NO), tumor necrosis factor (TNF-${\alpha}$), and prostaglandin $E_2$ ($PGE_2$) in LPS-induced Raw 264.7 cells. These results suggest the anti-inflammatory activity of PME could be due to the down-regulation of iNOS, COX-2, TNF-${\alpha}$, and $PGE_2$ in activated Raw 264.7 cells through NF-${\kappa}B$-dependent pathways.

Spectroscopic and Computational Insight into the Intermolecular Interactions between Zwitter-Type Ionic Liquids and Water Molecules

Park, HoSeok,Jung, Young Mee,Yang, Seong Ho,Shin, Weonho,Kang, Jung Ku,Kim, Hoon Sik,Lee, Hyun Joo,Hong, Won Hi WILEY-VCH Verlag 2010 Chemphyschem Vol.11 No.8

<P>Geometric and conformational changes of zwitter-type ionic liquids (ZILs) due to hydrogen-bonding interactions with water molecules are investigated by density functional theory (DFT), two-dimensional IR correlation spectroscopy (2D IR COS), and pulsed-gradient spin-echo NMR (PGSE NMR). Simulation results indicate that molecular structures in the optimized states are strongly influenced by hydrogen bonding of water molecules with the sulfonate group or imidazolium and pyrrolidinium rings of 3-(1-methyl-3-imidazolio)propanesulfonate (1) and 3-(1-methyl-1-pyrrolidinio)propanesulfonate (2), respectively. Concentration-dependent 2D IR COS reveals kinetic conformational changes of the two ZIL–H<SUB>2</SUB>O systems attributable to intermolecular interactions, as well as the interactions of sulfonate groups and imidazolium or pyrrolidinium rings with water molecules. The dramatic changes in the <SUP>1</SUP>H self-diffusion coefficients elucidate the formation of proton-conduction pathways consisting of ZIL networks. In ZIL domains, protons are transferred by a Grotthuss-type mechanism through formation, breaking, and restructuring of bonds between ZILs and H<SUB>2</SUB>O, leading to an energetically favorable state. The simulation and experimental investigations delineated herein provide a perspective to understanding the interactions with water from an academic point of view as well as to designing ILs with desired properties from the viewpoint of applications.</P> <B>Graphic Abstract</B> <P>Formation, breaking, and restructuring of bonds between water and zwitter-type ionic liquids (ZILs) such as 3-(1-methyl-3-imidazolio)propanesulfonate (see picture) are investigated by DFT calculations, 2D IR correlation spectroscopy, and pulsed-gradient spin-echo NMR. It is shown that in ZIL domains, protons are transferred by a Grotthuss-type mechanism. <img src='wiley_img_2010/14394235-2010-11-8-CPHC200900925-content.gif' alt='wiley_img_2010/14394235-2010-11-8-CPHC200900925-content'> </P>

Effect of Melatonin on Altered Expression of Vasoregulatory Genes during Hepatic Ischemia/Reperfusion

Park, Sang-Won,Choi, Sun-Mi,Lee, Sun-Mee 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.12

The production of reactive oxygen species during hepatic ischemia/reperfusion (I/R) can help create disturbances in microcirculation. This study examined the effect of melatonin, a pineal secretory product and a potent antioxidant, on the expression of vascular stress genes during hepatic I/R. Rats were subjected to 60 min of hepatic warm ischemia followed by 5 h reperfusion. Melatonin (10 mg/kg) was administered intraperitoneally 15 min before ischemia and immediately before reperfusion. The serum alanine aminotransferase and hepatic malondial-dehyde levels increased markedly after I/R. These increases were significantly inhibited by melatonin. The levels of endothelin-1 (ET-1) and its receptor, $ET_B$ mRNA, were elevated by I/R but attenuated by melatonin. The mRNA levels of endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), and heme oxygenase-1 were significantly higher after I/R. Melatonin augmented the increase in the eNOS mRNA level, whereas it reduced the increase in the iNOS mRNA level. The expression of tumor necrosis $factor-{\alpha}$ was increased markedly by I/R. This increase was also attenuated by melatonin. These results suggest that melatonin ameliorates the imbalanced expression of the vascular stress genes during hepatic I/R through its antioxidant property.

Trend Analysis and Prediction of Hepatobiliary Pancreatic Cancer Incidence and Mortality in Korea

Park Hyeong Min,Won Young-Joo,Kang Mee Joo,Park Sang-Jae,Kim Sun-Whe,Jung Kyu-Won,Han Sung-Sik 대한의학회 2022 Journal of Korean medical science Vol.37 No.28

Background: This study aimed to analyze the current trends and predict the epidemiologic features of hepatobiliary and pancreatic (HBP) cancers according to the Korea Central Cancer Registry to provide insights into health policy. Methods: Incidence data from 1999 to 2017 and mortality data from 2002 to 2018 were obtained from the Korea National Cancer Incidence Database and Statistics Korea, respectively. The future incidence rate from 2018 to 2040 and mortality rate from 2019 to 2040 of each HBP cancer were predicted using an age-period-cohort model. All analyses, including incidence and mortality, were stratified by sex. Results: From 1999 to 2017, the age-standardized incidence rate (ASIR) of HBP cancers per 100,000 population had changed (liver, 25.8 to 13.5; gallbladder [GB], 2.9 to 2.6; bile ducts, 5.1 to 5.9; ampulla of Vater [AoV], 0.9 to 0.9; and pancreatic, 5.6 to 7.3). The age-standardized mortality rate (ASMR) per 100,000 population from 2002 to 2018 of each cancer had declined, excluding pancreatic cancer (5.5 to 5.6). The predicted ASIR of pancreatic cancer per 100,000 population from 2018 to 2040 increased (7.5 to 8.2), but that of other cancers decreased. Furthermore, the predicted ASMR per 100,000 population from 2019 to 2040 decreased in all types of cancers: liver (6.5 to 3.2), GB (1.4 to 0.9), bile ducts (4.3 to 2.9), AoV (0.3 to 0.2), and pancreas (5.4 to 4.7). However, in terms of sex, the predicted ASMR of pancreatic cancer per 100,000 population in females increased (3.8 to 4.9). Conclusion: The annual incidence and mortality cases of HBP cancers are generally predicted to increase. Especially, pancreatic cancer has an increasing incidence and will be the leading cause of cancer-related death among HBP cancers.

Distinct prognosis of biliary tract cancer according to tumor location, stage, and treatment: A population-based study

Mee Joo KANG,Jiwon LIM,Sung-Sik HAN,Hyeong Min PARK,Sun-Whe KIM,Woo Jin LEE,Sang Myung WOO,Young-Joo WON,Sang-Jae PARK 한국간담췌외과학회 2022 Annals of hepato-biliary-pancreatic surgery Vol.26 No.-

Mackerel-derived fermented fish oil protects skin against UVB-induced cellular damage by inhibiting oxidative stress

Park, Jeong Eon,Hyun, Yu Jae,Piao, Mei Jing,Kang, Kyoung Ah,Ryu, Yea Seong,Shilnikova, Kristina,Zhen, Ao Xuan,Ahn, Mee Jung,Ahn, Yong Seok,Koh, Young Sang,Kang, Hee Kyoung,Hyun, Jin Won Elsevier 2018 Journal of Functional Foods Vol.46 No.-

<P><B>Abstract</B></P> <P>This study investigated the protective effect of mackerel-derived fermented fish oil (FFO) against UVB radiation-induced oxidative stress in human HaCaT keratinocytes and mouse skin tissue. FFO treatment scavenged UVB-induced intracellular reactive oxygen species and attenuated oxidative modifications including lipid peroxidation, protein carbonylation, and DNA damage. FFO treatment reduced UVB-induced apoptosis by reducing DNA fragmentation, caspase activation, and proapoptotic protein expression. UVB radiation activated phospho-extracellular signal-regulated kinase, phospho-c-Jun N-terminal kinase, and phospho-p38, whereas their specific inhibitors with FFO treatment abrogated the cell viability and apoptosis increased by UVB irradiation. FFO was more cytoprotective than docosahexaenoic acid, the main component of fish oil, against UVB exposure. Furthermore, the cytoprotective effect of FFO was evident in both UVB-exposed HaCaT cell and mouse models. Overall, these results demonstrate that FFO protects the skin against UVB-induced oxidative stress through antioxidant effects. FFO has the potential for development as a functional food against UVB-induced skin damage.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Mackerel-derived fermented fish oil (FFO) scavenged UVB-induced intracellular reactive oxygen species. </LI> <LI> FFO treatment attenuated UVB-induced oxidative skin cellular modifications and apoptotic cell death. </LI> <LI> FFO may be developed as a functional food against UVB-induced skin damage. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>FFO prevented epidermal damage including oxidative cellular damage and apoptotic cell death by directly inhibiting UVB radiation or decreasing ROS level induced by UVB radiation.</P> <P>[DISPLAY OMISSION]</P>