위상피세포에서 Helicobacter pylori 형태 변화가 Interleukin-8 분비에 미치는 영향

이학성,김혜원,홍원선,민영일,정훈용,김해련,양석균,이미화 대한소화기학회 1999 대한소화기학회지 Vol.33 No.6

Background/Aims: Interleukin-8 (IL-8) has been reported to play a critical role in Helicobacter pylori (H. pylori)-associated gastric mucosal damage. H. pylori exist in both bacillary and coccoid forms in the stomach. In contrast to bacillary forms, it is not clear whether coccoid forms stimulate gastric epithelial cells to produce IL-8. This study was conducted to investigate the effects of coccoid forms on IL-8 production. Methods: H. pylori strains of ATCC 43504, ATCC 43526 and three clinical isolates were used in the present study. Coccoid forms were induced by culturing bacillary forms of H. pylori for more than 84 hr. After co-culture of two human gastric adenocarcinoma cel lines (KATO III and AGS) with five strains of H. pylori, the levels of IL-8 were determined in th supernatants by enzyme linked immunosorbent assay. Results: The levels of IL-8 in KATO III and AGS cells were markedly elevated up to 6-9 hr after co-culture with the bacillary forms. The IL-8 levels produced in both cell lines by the coccoid forms were significantly lower than those by the bacillary forms in all strains. Conclusions: These results suggest that coccoid forms are much less implicated in IL-8- mediated gastric mucosal damage than bacillary forms.

사람 위선암에서 cathepsin L의 발현증가

홍원선,홍석일,박인철,손영숙,정훈용,양석균,김해련,민영일 울산대학교 의과대학 1996 울산의대학술지 Vol.5 No.1

cathepsin L은 lysosomal cysteine 단백분해효소로서 기저막(basement membrane)과 세포외기질(extracellular matrix)을 파괴하여 암세포의 침윤과 전이에 중요한 역할을 하는 물질로 알려져 있다. 이러한 cathepsin L에 대한 mRNA 발현도를 5개의 사람 위선암(gastric adenocarcinoma) 세포주와 5명의 위선암 환자에서 채취한 조직에서 방사능으로 표지된 cathepsin L특이 cDNA를 사용한 Northern blot법으로 측정하였다. 위암의 전이병소에서 수립한 세포주인 SNU-5, SNU-16, MKN-45와 Kato Ⅲ에서는 cathepsin L mRNA가 발현되었으나 원발병소에서 수립한 AGS 세포주에서는 mRNA의 발현이 관찰되지 않았다. 5명의 위암 환자에서는 원발병소, 전이가 확인된 임파절 및 암 근처 정상 위점막에서 각각 조직을 채취하여 cathepsin L mRNA의 발현을 측정하였다. 원발병소와 전이병소에서는 모두 cathepsin L mRNA가 발현되었으나 정상 위점막조직에서는 전예에서 mRNA 발현이 관찰되지 않았다. 한편 mRNA의 발현도는 1예에서는 전이병소가 원발병소에 비해 높았으나, 2예에서는 전이병소에서 발현도가 낮았으며, 나머지 2예에서는 원발병소와 전이병소 사이에 차이가 없어, 원발병소와 전이병소 사이에 mRNA의 발현도의 일관성 있는 경향은 관찰되지 않았다. 이상의 결과는 cathepsin L은 위암의 발생과 진행에 있어 암세포의 침윤과 전이를 촉진하는 것 이외에 또 다른 역할을 할 가능성을 시사하고 있다고 사료된다. Cathepsin L, a lysosomal cysteine protease, is known to play an important role in cancer invasion and metastasis by degrading the components of basement membrane and extracellular matrix. The mRNA expression of cathepsin L was determined by Northern blot analysis using a radiolabeled cDNA specific for cathepsin L in five human gastric adenocarcinoma cell lines and five surgical specimens of primary gastric adenocarcinomas, their metastatic lymph nodes and matched adjacent normal mucosae. The mRNA of cathepsin L was expressed in all of the four cell lines established from the metastatic sites, SNU-5, SNU-16, MKN-45 and Kato Ⅲ, while not detected in one cell line established from the primary site, AGS. The mRNA was expressed in all of the five primary and five metastatic cancer specimens tested, while it was not detected in all matched normal mucosae. The intensities of the mRNA expressions, however, did not show the consistent pattern between primary sites and metastatic lymph nodes. These results suggest that cathepsin L may have the other function in addition to facilitation of the invasion and metastasis during the development and progression of stomach cancer.

Aspergillus flavus에 의한 Versicolorin C의 생합성

서일원,조성환 慶尙大學校 1988 論文集 Vol.27 No.2

Potato dextrose agar상에 배양. 보관중인 Aspergillus flavus 를 Reddy's synthetic high -salts medium에 배양하고 균체로 부터 효소를 추출 분리한다음, ³H-Versicolorin A로 부터 화학 반을을거쳐 합성한 ³H-Versicolorin A hemiacetal을 기질로 하여 invitro 효소 반응을 시켰다.효소반응 결과 얻어진 반응생성 물질을 Thin layer Chromatography법으로 분리하고 표준화합물을 이용하여 비교동정하는 한편, 각 반응산물의 radiochromatogram를 작성하고, 추출. 분리한 화합물의 mass spctrometry 분석 결과, mass No 297,311,325,340에서 Peak를 가지는 Versicolorin의 존재를 확인. 동정할 수 있었다. 균체의 효소반응 실험결과, 조효소 무첨가구에서 (대조구에서)9.2%, NADH첨가구에서 10.3%,NADH첨가구에서 13.4%로 Versicolorin A hemiacetal이 Versicolorin C로 전환하는 것을 알 수 있어,NADPH가 이 환원반응을 촉진하는 reductase의 조효소작용에 관여함을 추정할 수 있었다. 따라서 포화 ammonium sulfate 용액으로 침전시켜 DEAE.Tris acryl M column chromatography에 의하여 정제, 분리한 효소 versicolorin A hemiacetal reductase의 spectrophotometric assay 결과에서도 NADH첨가구의 경우,2.67nmole/min의 효소 활성도를 관찰할 수 있어 Aspergillus flavus에 의한 versicolorin C의 생합성 기작을 확인할 수 있었다. Versicolorin A hemiacetal was converted to versicolorin Cin crude extracts and purified preparations from Aspergillus flavus. NADPH was required in the cell-free system. The rate of VAOH-dependent oxidation of NADPH was 0.43 mole/min/mg.protein. The substrate wa reduced in the cell-free system to a polar compound, proposed to be the open chain form. The polar compound was converted to the final product, versicolorin C.

물과 Micelle 용액에서 Benzaldehyde의 전기화학적 환원에 대한 연구

金日光,金潤根,金峯元,印權植 圓光大學校 基礎自然科學硏究所 1988 基礎科學硏究誌 Vol.7 No.3

The electrochemical reduction of benzaldehyde in 10% ethanol-water solution contained surfactants has been studied by direct current (DC) differential pulse (DP) polarography, cyclic voltammetry (CV) and controlled potential coulometry (CPC). A totally irreversible electrode reaction processes of benzaldehyde reduction proceeded by one electron transfer to form anion radical which undergoes both protonation and a second one electron reduction to give benzyl alcohol as a major product. The cathodic reduction wave was seperated by inhibition of sodium lauryl sulfate due to selectivity and effect of anionic charged micelle. The possible reduction mechanism with controlled pH range was suggested.

中國經濟發展에 對한 分析 : 毛澤東時期의 經濟 The economy the Mao's time

李元福,徐在日 대구대학교 경제경영연구소 2002 경제경영연구 Vol.1 No.2

毛澤東을 中心으로 수도인 北京에서 49년 10월 1일 中華人民共和國은 탄생함과 동시에 共産黨指導部는 국가체제를 정비하고 경제발전을 위하여 大躍進運動을 비롯한 計劃經濟開發을 단행하였다. 이시기 計劃經濟모델은 東獨과 蘇聯이었고, 蘇聯의 지원을 받아 1∼2차 經濟開發은 성공리에 끝났지만 文化大革命이라는 10년 정체기를 통하여 集團體制經濟는 落後의 길을 걷는다. 이러한 毛澤東時期의 經濟는 현 시기의 중국경제학자들에도 批評을 받는 부분이다. 비록 左派的인 路線에서 입각한 경제활동이었지만, 이 시기의 경제활동은 中國의 工業을 現代化시키기에 충분한 노력을 하였고, 이에 따른 成果와 이어지는 등소평시기의 경제적인 기초를 확립하였기 때문에 毛澤東時期의 經濟는 재조명해 보아도 충분한 價値가 있다고 할 것이다.

β-Cyclodextrine과 Micelle의 비수용액에서 Nitrobenzyl chlorides의 전기화학적 환원에 관한 연구

金日光,金峰元 圓光大學校 基礎自然科學硏究所 1984 基礎科學硏究誌 Vol.3 No.2

The electrochemical reduction of nitrobenzyl chloride in micelle and β-cyclodextrine acetonitrile solution has been examined by DC. DP. polarography and cyclic voltammetry. The polarographic reduction wave on the chloride functional group of nitrobenzyl chlorides shown to be eliminate in the anionic surfactant NaLS solution. In the β-cyclodextrine solution, on the other hand, nitro functional group of nitrobenzyl chlorides(gust) complexion does with β-cyclodextrine (host) with the aromatic ring inside the apolar cavity of the torus, result in dramatic eliminates on the nitro functional group reduction wave. o,m,p-nitrobenzyl chlorides are initially on one-electron process which give the corresponding anion radical and the anion radical is rapidly decomposed to give the neutral radical. Those neutral radical dimerises as a major product although some o,p-nitrotoluene is also produced by hydrogen atom abstraction from the solvent, in a β-cyclodextrine at low voltage. But in the NaLS solution produced some aminotoluene because of its charge repulsion effect.

함암요법으로 골수기능이 억제된 악성종양환자에서 rhG-CSF의 임상적 효과

김원민,서영환,조경상,유병전,김상도,이승일,정춘해 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

We have studied the efficacy of rhG-CSF in patients with non-hodgkin's lymphoma, acute leukemia, and small cell lung cancer undergoing anticancer chemotherapy. These patients were below leukocyte count 3,000 cubic millimeter due to myelosuppression induced by the first cycle of intensive chemotherapy. Treatment with rhG-CSF (100㎍ per square meter of body surface area per day in a 30-minute intravenous infusion) was begun two days and for 14 consecutive days after the end of the second cycle of chemotherapy. The results were as follows. 1. The onset of myelosuppression was 6 days after chemotherapy, and the onset of recovery was 16.7 days after chemotherapy, and the duration of granulocytopenia was 10.7 days in patients with malignant tumor during contrast period. 2. The duration of granulocytopenia was shortened 5.2 days in patients administered rhG-CSF than without rhG-CSF, and we observed the shortest duration of granulocytopenia with increasing granulocyte in patients with small cell lung cancer. 3. Observing the differential count of leukocyte checked the highest level of leukocyte in contrast and rhG-CSF period, the persentage of neutrophil was increased in patients with acute leukemia and small cell lung cancer during rhG-CSF period, but not increased in patients with non-hodgkin's lymphoma. 4. rhG-CSF was not influenced on liver faction, renal fuction, uric acid, and glucose metabolism, also had no effect on recovery of platelet. In conclusion, rhG-CSF can be administered to patients with hematologic or nonhematologic malignant tumor that myelosuppression induced by anticancer chemotherapy result in shortening the duration of granulocytopenia and increasing the peripheral neutrophil, therefore full dose chemotherapy can be administered on time, and rhG-CSF may reduce the morbidity and mortality of patients with malignant tumor undergoing chemotherapy.

中國經濟發展에 對한 分析 : 鄧小平時期의 經濟 The economy time the Deng's time

李元福,徐在日 대구대학교 경제경영연구소 2003 경제경영연구 Vol.2 No.1

"文革"기간동안 四人幇에 의해 강소성의 한 공장에서 노동을 하다가 1973년 복직한 鄧小平은 1978년부터 중국 공산당의 주요 정책 결정요인이 되면서, 그는 전당 제정과 새로운 발전 시기의 노선, 방침, 정책 실시를 이끌어 나갔다. 그는 解放思想, 實事求是, 앞을 내다보는 방침으로 단결 일치하여 "사회주의 노선, 인민민주주의전권, 공산당지도자들, 마르크스 레닌주의, 毛澤東 사상의 4가지 기본원칙의 견지"할 것을 주장하고, 兩岸間의 통일대업에 만은 관심을 기울여, "하나의 국가, 두개의 제도"라는 방안을 제시하였다. 중국은 1978년 제11기 3중전회 직후 개최된 경제학회에서 "市場經濟"의 문제가 제기하였고, 1984년 중국공산당 제 12기 3中全會에서 "경제개혁에 관한 결정"을 통과시켰다. 이 경제개혁에 관한 결정에서는 사회주의경제를 '생산요소의 사회주의 공유제에 기초한 계획적인 상품경제 라고 정의했으며, 당시 저명한 경제학자들은 사회주의 상품경제는 곧 사회주의 시장경제라고 칭할 수 있다'라는 의견을 제기하였다. 중국 개혁·개방의 경과 및 성과를 살펴보면 1978년 12월 중국은 대외개방을 천명한 11기 3中全會 이후 시장기능을 적극 도입함으로써 "중국특색의 사회주의 시장경제 건설"을 통한 경제발전을 이루기 위해 노력해 왔다. 그 결과 중국은 최근 몇 년간에 12~13%의 높은 경제성장을 이룩하였고, 중국의 수출액은 94년 1,210억 달러로 세계 9위를 기록하고 있다. 또 중국의 수입액도 94년 1,157억 달러로 9위를 기록하고 있으며 대외무역관계의 계속적인 확대로 현재 180여 개 이상의 국가와 교역관계를 맺고 있는 것으로 보인다. 특히 선진국에 대한 수출입 비중이 점차 높아지고 있는 등 고도성장을 계속하고 있다. 鄧小平정권의 과감한 개혁과 개방을 추진한 결과, "사회주의 시장경제"로 전환하면서 비약적인 경제발전을 성취함으로써 세계의 주목을 받는 경제대국으로 등장하였다. Deng Xiaoping who resumed office in 1973 after having been expelled by 四人幇 and doing manual labor during the Cultural Revolution rose as a decision maker of major policies of Chinese Communist Party and led the new plan and policy line of the party. He established such perspective policies as liberation idea and the idea of doing the practical and leading the right, and asserted that the party adhere to the line of socialism, the right of people s democracy, the leaders of the Communist Party, Marxism .-Leninism and the four basic principles of Mao Zedong s idea. And also he was much interested in unification between China and Taiwan and presented the policy of "one country, two systems". The problem of market economy was carried into an economic conference shortly after the 3rd national convention of Chinese Communist Party was held in 1978. And the national convention of the party passed "the decision on economic revolution" in 1884 Socialism economy was defined as merchandise economy which was based on the system of socialism public-ownership of production factors. Distinguished economists who attended the convention raised an opinion that the socialism merchandise economy could be called socialism market economy. China has tried to achieve economic growth through construction of socialism market economy which was characteristic in China by adopting the function of market actively since it announced foreign open in the national convention of the party on December. 1978. As a result, China has achieved high economic growth by 12-13% for past several years. Its exports was 121,000,000,000 dollars in 1994, becoming 9th exporting country in world trade. And its imports 115,700,000,000 dollars, becoming 9th importing country in world trade in 1994. It has traded with more than 180 countries. thanks to continuous extension of international trade. Especially, it has achieved high economic growth by increasing exports and imports with advanced countries. Deng Xiaoping s determined revolution and open policy changed China's economy socialism into market economy, and China rose as an economic power by achieving great economic development.

응급실에 내원한 환자에서 어지럼증의 원인과 임상양상의 분석

문원식,정경운,위준선,문정미,전병조,김용권,소정일,류진호,허탁,민용일 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: Recently, The number of patients who have been complaining of the vertigo or dizziness has been increasing due to rapid growth of elderly population and senile disease. The differentiation of dizzy patients is not familiar to most emergency physicians. This study was designed to differentiate true vertigo and to investigate the clinical difference among central vertigo, peripheral vertigo, and other causes of dizziness. Methods: The authors analyzed the cases of 237 dizzy patients who visited the emergency department of Chonnam University Hospital during the recent 2 years. For the base of dizziness, associated past illnesses, severity, nystagmus type, and the causes of central and peripheral vertigo. Results: Female patients were 142(59.9%) and male patients were 95(40.1%). The most common age group was the 6th decade. The origin of dizziness was classified as peripheral cause(25.3%), central cause(32.9%), and others(41.8%). As to nature of the dizziness, the rotatory sense was dominant in peripheral vertigo and the floating sense and blurred vision in central vertigo. Peripheral vertigo was triggered by position change of head and body, and central vertigo by the eye movement. Audiograms I showed that most patients with central vertigo had normal hearing, but 46.7% of thoswith peripheral vertigo had an abnormal hearing disturbance. Nystagmus was more prevalent in peripheral vertigo Conclusion: History taking and physical examination played an important role in the diagnosis of dizzy patients. An important part of the diagnosis of a dizzy patient could be to evaluate the peripheral origin, the central origin and others causes. Because central vertigo may be associated with a significant neurological pathology, spectral radiological studies, including MRI and CT, are mandatory to rule out a devastating brain lesion.

평야지재배 조생종 벼의 이화학적 및 식미특성 분석

정진일,김기영,최윤희,최원영,고종철,오명규,홍하철,이승엽,이명철 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.4

평야지재배 적응 조생종 벼 품종의 선발 및 품종 육성의 기초 자료로 활용코자 극조생종(1품종)과 조생종(10품종) 및 준조생(3품종)등 총 14품종을 산간지(운봉)와 평야지(익산)에 지역별 표준재배법으로 공시한 결과, 1. 극조생종 및 조생종들은 감온성이 커 산간지에서 출수가 빠르나, 준조생종에 속하는 상미벼, 영덕34호, 히또메보레 등은 감광성이 감온성보다 커, 평야지재배에서 4일정도 빨리 출수하였다. 2. 수량성은 대부분의 품종들이 산간지재배에 비해 평야지 재배에서 높았고 대체로 출수가 빠른 품종일수록 지역간의 수량차가 크게 나타났으며, 출수가 다소 늦은 준조생종들은 산간지와 평야지의 재배에서 수량차가 크지 않아 산간지 뿐만 아니라 평야지에서도 적응성이 높았다. 3. 단백질함량은 숙기가 다소 늦은 품종일수록 낮은 경향을 보였고 산간지보다는 평야지에서 낮았으며, 품종간에는 오대벼, 운봉30호, 영덕34호, 신석조생, 상미벼 등이 함유량이 낮았다. 4. 식미치는 산간지재배가 평야지에 비해 보다 높게 나타났고, 출수가 늦을수록 높았으며, 지역간 차가 크게 나타난 품종은 극조생종인 키라라397이었고, 출수가 늦은 상미벼와 히또메보레는 차가 적었다. 5. 산온도와 수량과의 상관은 산간지에서만 영향이 있을 뿐 평야에서는 영향이 적고 일사량과 일조시수가 수량에 보다 상관성이 높았으며, 식미치는 적산온도와는 부의상관, 생육 일수와는 정상관을 보였다. This experiment was carried out to clarify the difference of rice yield and the quality of rice in southern plan area and in alpine area. Yield and quality of fourteen early varieties were investigated under the different transplanting dates and planting densities in southern plan area(Iksan) and in alpine area(Unbong). In the alpine area, extremely early variety and early varieties headed early compared to the plain area, but medium early varieties headed about 4 days late unlike the alpine area. The yield difference of most early varieties including Kilara 397 of extremely early flowering variety were greater in plain area than in alpine. The different of yield between plain and alpine area was less in varieties with Sinseogjosaeng, Odaebyeo, Akidagomachi, and Yeungdeuk34. The rate of protein was lower in plain area than alpine and more lately flowering than early, but palatability of milled rice was higher at the alpine area and more lately flowering varieties. Correlation coefficients between yield and meterological data during maturity, yield shower the significant correlation with integrated solar radiation and sunshine hours at cultured in southern plan area, but it showed only cumulative temperature in alpine area. Palatability of miller rice was positive correlation at growth duration but negative correlation at cumulative temperature.