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Wen Zhu,Wenfeng Wang,Wencan Xu,Shuang Wu,Wenjun Chen,Youyi Huang,Shengpeng Wang 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.7
Pile-fermentation is a critical procedure for producing Chinese dark tea, during which thermophilic microorganisms would play an irreplaceable role. However, there have been little researches on the influences of thermophilic microorganism pile-fermentation (TMPF) in high-temperature of Chinese dark tea. Thus, we conducted high-performance liquid chromatography and nontargeted metabolomic to analyze the non-volatile metabolites of TMPF. Our results discovered that the amounts of ( −)-epigallocatechin gallate, ( −)-epigallocatechin, ( −)-epicatechin gallate, and ( −)-epicatechin were decreased significantly (p < 0.05) after TMPF. By using nontargeted metabolomic analysis, a total of 1733 ion features were detected. KEGG pathway enrichment analysis showed that TMPF had a significant impact on caffeine metabolism. Also, theophylline, 3-methylxanthine, and 1,3,7-trimethyluric acid were increased significantly after TMPF, which suggested that demethylation and oxidation reaction might be the main pathways of caffeine metabolism. This study provides a better understanding of the mechanism of TMPF during high-temperature for Chinese dark tea and lays a foundation for further research.
( Wenzhu Zhang ),( Kyung Sup Kwak ),( Chengxiao Feng ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.7
In order to guide users to select the most optimal access network in heterogeneous wireless networks, a network selection algorithm is proposed which is designed based on multi-objective discrete particle swarm optimization (Multi-Objective Discrete Particle Swarm Optimization, MODPSO). The proposed algorithm keeps fast convergence speed and strong adaptability features of the particle swarm optimization. In addition, it updates an elite set to achieve multi-objective decision-making. Meanwhile, a mutation operator is adopted to make the algorithm converge to the global optimal. Simulation results show that compared to the single-objective algorithm, the proposed algorithm can obtain the optimal combination performance and take into account both the network state and the user preferences.
Wenzhu Dai,Jixiang He,Ling Zheng,Mingyu Bi,Fei Hu,Minju Chen,Heng Niu,Jingyu Yang,Ying Luo,Wenru Tang,Miaomiao Sheng 한국유방암학회 2019 Journal of breast cancer Vol.22 No.2
Purpose: Breast cancer is the most frequently diagnosed malignancy in women worldwide. MicroRNAs (miRNAs) are thought to serve as potential biomarkers in various cancers, including breast cancer. Methods: We evaluated the miRNA expression profiles in 1,083 breast cancer samples and 104 normal breast tissues from The Cancer Genome Atlas database. We used the edgeR package of R software to analyze the differentially expressed miRNAs in normal and cancer tissues, and screened survival-related miRNAs by Kaplan-Meier analysis. A receiver operating characteristic curve was generated to evaluate the accuracy of these miRNAs as molecular markers for breast cancer diagnosis. Furthermore, the functional role of these miRNAs was verified using cell experiments. Targets of candidate miRNAs were predicted using 9 online databases, and Gene Ontology (GO) functional annotation and pathway analyses were conducted using Database for Annotation, Visualization and Integrated Discovery online tool. Results: A total of 68 miRNAs showed significantly different expression patterns between the groups (p < 0.001), and 13 of these miRNAs were significantly associated with poor survival (p < 0.05). Three miRNAs with high specificity and sensitivity, namely, miR-148b-3p, miR-190b, and miR-429, were selected. In vitro experiments showed that the overexpression of these 3 miRNAs significantly promoted the proliferation and migration of MDA-MB-468 and T47D cells and reduced the apoptosis of T47D cells. GO and pathway enrichment analyses revealed that the targets of these dysregulated miRNAs were involved in many critical cancer-related biological processes and pathways. Conclusion: The miR-148b-3p, miR-190b, and miR-429 may serve as potential diagnostic and prognostic markers for breast cancer. This study demonstrated the roles of these 3 miRNAs in the initiation and progression of breast cancer.
Wenzhu Jiang,이주현,Yong-Mei Jin,Yongli Qiao,Rihua Piao,Sun Mi Jang,우미옥,권순욱,Xianhu Liu,Hong-Yu Pan,Xinglin Du,고희종 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.4
Seed germination capability of rice is one of the impor-tant traits in the production and storage of seeds. Quantitative trait loci (QTL) associated with seed germination capability in various storage periods was identified using two sets of recombinant inbred lines (RILs) which derived from crosses between Milyang 23 and Tong 88-7 (MT-RILs) and between Dasanbyeo and TR22183 (DT-RILs). A total of five and three main additive effects (QTLs) associated with seed germination capability were identified in MT-RILs and DT-RILs, respectively. Among them, six QTLs were identified repeatedly in various seed storage periods designated as qMT-SGC5.1, qMT-SGC7.2, and qMT-SGC9.1 on chro-mosomes 5, 7, and 9 in MT-RILs, and qDT-SGC2.1, qDT-SGC3.1, and qDT-SGC9.1 on chromosomes 2, 3, and 9 in DT-RILs, respectively. The QTL on chromosome 9 was identified in both RIL populations under all three storage periods, explaining up to 40% of the phenotypic variation. Eight and eighteen pairs additive additive epistatic effect (epistatic QTL) were identified in MT-RILs and DT-RILs, respectively. In addition, several near isogenic lines (NILs) were developed to confirm six repeatable QTL effects using controlled deterioration test (CDT). The identified QTLs will be further studied to elucidate the mechanisms controlling seed germination capability, which have important implications for long-term seed storage.
Wenzhu Jiang,Yong-Mei Jin,이주현,Kang-Ie Lee,Rihua Piao,Longzhi Han,Jin-Chul Shin,Rong-De Jin,Tiehua Cao,Hong-Yu Pan,Xinglin Du,고희종 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.6
Low temperature is one of the major environmental stress-es in rice cultivation in high-altitude and high-latitude regions. In this study, we cultivated a set of re-combinant inbred lines (RIL) derived from Dasanbyeo (indica) / TR22183 (japonica) crosses in Yanji (high-latitude area), Kunming (high-altitude area), Chuncheon (cold water irrigation) and Suwon (normal) to evaluate the main effects of quantitative trait loci (QTL) and epistatic QTL (E-QTL) with regard to their interactions with environments for cold-related traits. Six QTLs for spikelet fertility (SF) were identified in three cold treatment locations. Among them, four QTLs on chromosomes 2, 7, 8, and 10 were validated by several near isogenic lines (NILs) under cold treatment in Chuncheon. A total of 57 QTLs and 76 E-QTLs for nine cold-related traits were identified as distributing on all 12 chromosomes; among them, 19 QTLs and E-QTLs showed significant interactions of QTLs and envi-ronments (QEIs). The total phenotypic variation explained by each trait ranged from 13.2 to 29.1% in QTLs, 10.6 to 29.0% in E-QTLs, 2.2 to 8.8% in QEIs and 1.0% to 7.7% in E-QTL environment interactions (E-QEIs). These results demonstrate that epistatic effects and QEIs are important properties of QTL parameters for cold tolerance at the reproductive stage. In order to develop cold tolerant varieties adaptable to wide-ranges of cold stress, a strategy facilitating marker-assisted selection (MAS) is being adopted to accumulate QTLs identified from different environments.
SIMULATION STUDY ON VEHICLE RIDE COMFORT BASED ON FINITE ELEMENT MODEL AND PSEUDO-EXCITATION METHOD
Wang Wenzhu,Lijie Dong,Liu Gang,Wei Jun,Zhang Zhenwei 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.4
This research aims to develop a new vehicle ride comfort simulation technology. The basic principle of pseudo-excitation method (PEM) is summarized, and the specific method of PEM is proposed to solve the finite element model (FEM) of vehicle. Taking a simple 7-degree-of-freedom (DOF) car spatial model as an example, the traditional and new methods are compared. Simulation results show that they are completely consistent, thereby verifying the effectiveness of the new method. At the same time, through the simulation process, the new method is simpler and more efficient without deducing complex mathematical formulas and software programming. Finally, the ride comfort simulation of a car’s complex FEM is performed. FEM can consider the parts of the vehicle as elastic bodies and closer to the real vehicle. The PEM transforms random vibration analysis into simple harmonic vibration analysis, which improves the efficiency of the solution greatly. Therefore, the combination of the FEM and the PEM cannot only solve the problem of model accuracy but also the problem of solving efficiency, which has strong practical engineering application value.
Sang-Ho Chu,Wenzhu Jiang,Joong-Hyoun Chin,Hee-Jong Koh 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Heterosis describes the increased performance of F1 hybrid plants in terms of increased biomass, yield, vegetative growth rate, and tolerance against biotic and abiotic stresses as compared with their inbred parents. Two sets of rice materials, 166 RILs derived from a cross between Milyang 23 (Korean indica-type rice) and Tong 88-7 (japonica Rice), and BC1F1 hybrids derived from crosses between the RILs and the female parent, Milyang 23, were produced to identify QTLs for heterosis of yield and yield-related traits. The QTLs were detected from three different phenotype data sets including the RILs, BC1F1 hybrids, and mid-parental heterosis data set. A total of 57 QTLs were identified for nine traits. Of eight QTLs detected for yield heterosis, five overlapped with other heterosis QTLs for yield-related traits such as spikelet number per panicle, days to heading, and spikelet fertility. Four QTLs for yield heterosis, gy1.1, py6, gy10, and py11, were newly identified in this study. We identified a total of 17 EpQTLs for yield heterosis that explain 21.4 ~ 59.0 % of total phenotypic variation, indicating that epistatic interactions may play an important role in heterosis.