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A Cognition-inspired System for Data Stream Clustering
Zhaoyang Sun,K. Z. Mao,Wenyin Tang,Lee-Onn Mak,Kuitong Xian,Ying Liu 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.8
In applications such as target detection, domain knowledge of sensed data is often available. In this paper, we incorporate the available domain knowledge into clustering process and develop a knowledge-driven Mahalanobis distance-based ART (adaptive resonance theory) clustering algorithm. The strength of the knowledge-driven algorithm is that it can automatically determine the number of clusters with improved clustering results. The validity of the new algorithm has been verified on four artificial datasets. In addition, the algorithm has been adopted in our cognition-inspired system for clustering data stream, where known target library and dispersion of feature or attributes are available. The basic idea of this system is to divide data stream into frames, and to incorporate knowledge learned in previous frames into clustering of the following ones. Experimental studies have demonstrated that the evolving learning mechanism leads to improved clustering results compared with conventional incremental clustering algorithm Fuzzy ART and batch-based clustering algorithm k-means.
Production of Recombinant Humanized Anti-HBsAg Fab Fragment from Pichia pastoris by Fermentation
Deng, Ning,Xiang, Junjian,Zhang, Qing,Xiong, Sheng,Chen, Wenyin,Rao, Guirong,Wang, Xunzhang Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.3
In this report, we describe the high-yield secretory expression of the recombinant human anti-HBsAg Fab fragment from Pichia pastoris that was achieved by co-integration of the genes encoding the heavy and light chains (both under the control of alcohol oxidase promoter) into the genome of the yeast cells. The fed-batch fermentations were carried out in a 5 L scale. Both chains of the Fab were successfully expressed upon methanol induction. The absorbance ($OD_{600}$) of the broth can reach 350~500 at the end of fed-batch phase. After the induction, the expression level of the recombinant Fab (soluble) reached 420~458 mg/L. The recombinant Fab fragment was purified from the crude culture supernatant by ion exchange chromatography and the purity of the recombinant Fab fragment was over 95%. The affinity activities of the crude fermentation supernatant and the purified Fab were analyzed by indirect ELISA, which showed that the purified recombinant Fab fragment had high affinity activity with hepatitis B surface antigen.