RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      선택해제
      • 좁혀본 항목 보기순서

        • 원문유무
        • 원문제공처
          펼치기
        • 등재정보
          펼치기
        • 학술지명
          펼치기
        • 주제분류
          펼치기
        • 발행연도
          펼치기
        • 작성언어
        • 저자
          펼치기

      오늘 본 자료

      • 오늘 본 자료가 없습니다.
      더보기
      • 무료
      • 기관 내 무료
      • 유료
      • KCI등재

        Influence of the silencing sex-peptide receptor on Bactrocera dorsalis adults and offspring by feeding with ds-spr

        Wenping Zheng,Yaru Liu,Weiwei Zheng,Yunli Xiao,Hongyu Zhang 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3

        RNA interference (RNAi) has been shown to control insect pests using plant dsRNA expression. A key question in potentially applying RNAi is the possible effects on insects after being fed with dsRNA produced by a transgenic plant. Sex peptide receptor (spr) is the key gene that regulates the suppression of female receptivity and induction of oviposition. In this study, the expression level of the spr genewas significantly down-regulated to 52% by feeding Bactrocera dorsalis adults with ds-spr. The RNAi effects of continuous feeding ds-spr to adults led to a highly mortality rate, decreased their egg production capacity and profoundly impacted the eclosion rate of their offspring. Our results demonstrate that RNAi through uninterrupted dsRNA feeding can be used as a strategy to control insect pests. Moreover, the research presented here provides a potential RNAi target gene for controlling B. dorsalis and a theoretical basis for universally applying RNAi in insect pest management.

      • KCI등재후보

        A Pilot Study Exploring the Effects of Reflexology on Cold Intolerance

        Wenping Zhang,Shougo Takahashi,Takashi Miki,Hisayo Fujieda,Torao Ishida 사단법인약침학회 2010 Journal of Acupuncture & Meridian Studies Vol.3 No.1

        Cold intolerance is an inability to tolerate cold temperatures and is accompanied by symptoms including headache, shoulder discomfort, dizziness and palpitations. The current study was performed to examine whether reflexology therapy affected cold intolerance in human subjects and whether the treatment was systemically effective. Ten female volunteer examinees with subjective feelings of cold were examined. After a 5-minute foot bath, 10 minutes of reflexology therapy was performed on their left foot. Skin temperature and blood flow were estimated before and after treatment, together with an interview concerning their feelings of cold and daily habits. In addition, how the recovery rate was affected by the application of a chilled-water load was also estimated. Along with significant increases in skin temperature and blood flow compared with pre-treatment at the bilateral points of KI-1, LR-3, and BL-60, a faster recovery after the application of the chilled-water load was also seen in the lower limbs on both sides. From these results, we conclude that reflexology has systemic effects and is an alternative method for treating cold intolerance.

      • KCI등재

        Transcriptome analysis of Dioscorea zingiberensis identifies genes involved in diosgenin biosynthesis

        Wenping Hua,Weiwei Kong,XiaoYan Cao,Chen Chen,Qian Liu,Xiangmin Li,Zhezhi Wang 한국유전학회 2017 Genes & Genomics Vol.39 No.5

        Dioscorea zingiberensis is the main plant source of diosgenin, a precursor for the production of steroid hormones used in the pharmaceutical industry. The extraction process of diosgenin from D. zingiberensis can generate high-acid and high-strength wastewater on a large scale and can threaten the environment. Bioengineering microorganisms to produce diosgenin is an effective way to avoid pollution. However, little is known about the genes that are involved in the biosynthesis of diosgenin. We obtained 85,010 unigenes (average length of 1142 bases) from the D. zingiberensis transcriptome through RNAseq. A large number of unigenes (59,368; 69.83%) were annotated, and 2488 unigenes were assigned to 27 secondary- metabolite pathways. In our database, 66 unigenes encoding up to 40 key enzymes were found to be present in diosgenin biosynthesis pathways. In addition, we found 203 unigenes encoding CYP450 proteins and 47 unigenes encoding UGT proteins that may be involved in modifications of a downstream pathway. The expression patterns of key diosgenin biosynthesis genes were studied to identify the most important members of the enzyme family. These results add to the available genetic data of D. zingiberensis and lay the foundation for the further production of diosgenin using genetic engineering.

      • KCI등재

        Effects of microwave and exogenous l-phenylalanine treatment on phenolic constituents, antioxidant capacity and enzyme inhibitory activity of Tartary buckwheat sprouts

        Wenping Peng,Nan Wang,Shunmin Wang,Junzhen Wang,Zixiu Bian 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.1

        The phenolic substances, antioxidant capacity, and enzyme inhibitory activity of germinated Fagopyrum tataricum (Tartary buckwheat) under different microwave and L-phenylalanine (L-Phe) were investigated for the potential of enriching polyphenols. With the germination of seeds, the contents of total phenolics and total flavonoids increased, the antioxidant capacity and enzyme inhibitory activity were enhanced. The highest contents of total phenolics and total flavonoids in Tartary buckwheat sprouts were 17.41 mg GAE/g and 6.26 g RE/100 g DW (7 days), respectively. Correlation analysis and principal component analysis indicated that T3 (microwave 250 W, 90 s; L-Phe 2.9 mmol/L) could effectively improve the content of polyphenols, enzyme inhibition activity and antioxidant capacity of Tartary buckwheat sprouts obviously. This study hopes to provide some new ideas for enriching phenolics and improving antioxidation of Tartary buckwheat sprouts.

      • KCI등재

        Production responses of Holstein dairy cows when fed supplemental fat containing saturated free fatty acids: a meta-analys

        Wenping Hu,Jacquelyn P. Boerman,James M. Aldrich 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8

        Objective: A meta-analysis was conducted to evaluate the effects of supplemental fat containing saturated free fatty acids (FA) on milk performance of Holstein dairy cows. Methods: A database was developed from 21 studies published between 1991 and 2016 that included 502 dairy cows and a total of 29 to 30 comparisons between dietary treatment and control without fat supplementation. Only saturated free FA (>80% of total FA) was considered as the supplemental fat. Concentration of the supplemental fat was not higher than 3.5% of diet dry matter (DM). Dairy cows were offered total mixed ration, and fed individually. Statistical analysis was conducted using random- or mixed-effects models with Metafor package in R. Results: Sub-group analysis showed that there were no differences in studies between randomized block design and Latin square/crossover design for dry matter intake (DMI) and milk production responses to the supplemental fat (all response variables, p≥0.344). The supplemental fat across all studies improved milk yield, milk fat concentration and yield, and milk protein yield by 1.684 kg/d (p<0.001), 0.095 percent unit (p = 0.003), 0.072 kg/d (p<0.001), and 0.036 kg/d (p<0.001), respectively, but tended to decrease milk protein concentration (mean difference = –0.022 percent unit; p = 0.063) while DMI (mean difference = 0.061 kg/d; p = 0.768) remained unchanged. The assessment of heterogeneity suggested that no substantial heterogeneity occurred among all studies for DMI and milk production responses to the supplemental fat (all response variables, I2≤24.1%; p≥0.166). Conclusion: The effects of saturated free FA were quantitatively evaluated. Higher milk production and yields of milk fat and protein, with DMI remaining unchanged, indicated that saturated free FA, supplemented at ≤3.5% dietary DM from commercially available fat sources, likely improved the efficiency of milk production. Nevertheless, more studies are needed to assess the variation of production responses to different saturated free FA, either C16:0 or C18:0 alone, or in combination with potentially optimal ratio, when supplemented in dairy cow diets.

      • KCI등재후보

        The Effect of the Major Components of Fructus Cnidii on Osteoblasts In Vitro

        Wenping Zhang,Dongming Ma,Qiduo Zhao,Torao Ishida 사단법인약침학회 2010 Journal of Acupuncture & Meridian Studies Vol.3 No.1

        In traditional Chinese medicine, the cause of weak bones or bone loss is generally regarded as a result of kidney deficiency. Fructus Cnidii (FC), which is also known as She-Chuang-Zi, is a traditional herb that has been claimed to have kidney warming effects that invigorate Yang. In this study, we tried to determine the bone production-inducing effect of FC on osteoblastic cells in vitro using osthole, the main component of FC. Osteoblasts were isolated from neonatal Sprague-Dawley rat calvaria using the tissue piece culture method and treated with various concentrations of osthole ranging from 2.5 to 640 μg/mL, together with a blank control. Cell proliferation,alkaline phosphatase (ALP) activity, and bone nodules were measured. The cells were examined by hematoxylin-eosin staining, the Gomori Calcium-Cobalt method and immunofluorescent staining. The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (or MTT) assay, ALP assay, and bone nodule results indicated significantly enhanced osteoblastic proliferation and differentiation at concentrations of osthole ranging from 40 to 320 μg/mL. Concentrations lower than 40 μg/mL seemed less effective, and cytotoxicity to osteoblasts was observed at concentrations higher than 320 μg/mL. These results indicate that osthole is effective at inducing osteoblastic bone formation through the up-regulation of ALP activity. FC is a Chinese herb used to treat lumbar pain in clinical practice. Further studies concerning the effects and mechanism of osthole on osteoporotic patients and animals should be performed, as these studies may lead to the development of a drug treatment for osteoporosis in the future.

      • KCI등재

        Shedding Light on the Use of AS Relationships for Path Inference

        Wenping Deng,Wolfgang Mühlbauer,Yuexiang Yang,Peidong Zhu,Xicheng Lu,Bernhard Plattner 한국통신학회 2012 Journal of communications and networks Vol.14 No.3

        Autonomous system (AS) business relationships and their inference have been widely studied by network researchers in the past. An important application of inferred AS relationships can be the prediction of AS paths between a source and destination AS within a model. However, besides knowing the topology and inferred AS relationships, AS path prediction within a model needs to be understood in order for us to know how we can derive border gateway protocol (BGP) policies from AS relationships. In this paper, we shed light onto the predictive capabilities of AS relationships by investigating whether they can be translated into BGP policies such that inferred AS paths are consistent with real AS paths, e.g., paths observed from BGP routing tables. Our findings indicate that enforcing constraints such as the well-known valley-free property and the widely assumed preference of customer routes always results in a very low consistency for AS path inference. In addition, this is true irrespective of whether customer,peer, or provider routes are preferred. Apparently, applying such constraints eliminates many “correct” paths that are observed in BGP routing tables and that are propagated in a simple shortest path model where AS relationships are ignored. According to our findings, deriving BGP routing policies for predicting with high accuracy AS paths in a model directly from AS relationships is still difficult.

      연관 검색어 추천

      이 검색어로 많이 본 자료

      활용도 높은 자료

      해외이동버튼