Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita

Wenjie Tong(Wenjie Tong),Junying Li(Junying Li),Wenfeng Cong(Wenfeng Cong),Cuiping Zhang(Cuiping Zhang),Zhaoli Xu(Zhaoli Xu),Xiaolong Chen(Xiaolong Chen),Min Yang(Min Yang),Jiani Liu(Jiani Liu),Lei Yu 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6

Root-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita-infected tobacco plants. We studied the rhizosphere bacterial community structure of M. incognita-infected and uninfected tobacco plants through a paired comparison design in two regions of tobacco planting area, Yuxi and Jiuxiang of Yunnan Province, southwest China. According to the findings, M. incognita infection can alter the bacterial population in the soil. Uninfested soil has more operational taxonomic unit numbers and richness than infested soil. Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. Firmicutes was prevalent in infested soil, but Chloroflexi and Acidobacteria were prevalent in uninfested soil. Sphingomonas, Streptomyces, and Bradyrhizobium were the dominant bacteria genera, and their abundance were higher in infested soil. By PICRUSt analysis, some metabolism-related functions and signal transduction functions of the rhizosphere bacterial community in the M. incognita infection-tobacco plants had a higher relative abundance than those uninfected. As a result, rhizosphere soils from tobacco plants infected with M. incognita showed considerable bacterial community structure and function alterations.

A Closed-Form Robust Chinese Remainder Theorem and Its Performance Analysis

Wenjie Wang,Xiang-Gen Xia IEEE 2010 IEEE transactions on signal processing Vol.58 No.11

<P>Chinese remainder theorem (CRT) reconstructs an integer from its multiple remainders that is well-known not robust in the sense that a small error in a remainder may cause a large error in the reconstruction. A robust CRT has been recently proposed when all the moduli have a common factor and the robust CRT is a searching based algorithm and no closed-from is given. In this paper, a closed-form robust CRT is proposed and a necessary and sufficient condition on the remainder errors for the closed-form robust CRT to hold is obtained. Furthermore, its performance analysis is given. It is shown that the reason for the robustness is from the remainder differential process in both searching based and our proposed closed-form robust CRT algorithms, which does no exist in the traditional CRT. We also propose an improved version of the closed-form robust CRT. Finally, we compare the performances of the traditional CRT, the searching based robust CRT and our proposed closed-form robust CRT (and its improved version) algorithms in terms of both theoretical analysis and numerical simulations. The results demonstrate that the proposed closed-form robust CRT (its improved version has the best performance) has the same performance but much simpler form than the searching based robust CRT.</P>

Exploring the impact of residents’ emotional solidarity on attitude and support for tourism development in the context of COVID-19

Wenjie Xu,Ju-Hee Lee,Choong-Ki Lee 한국관광연구학회 2020 관광연구저널 Vol.34 No.10

Constrained Relay Node Deployment using an improved multi-objective Artificial Bee Colony in Wireless Sensor Networks

( Wenjie Yu ),( Xunbo Li ),( Xiang Li ),( Zhi Zeng ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.6

Wireless sensor networks (WSNs) have attracted lots of attention in recent years due to their potential for various applications. In this paper, we seek how to efficiently deploy relay nodes into traditional static WSNs with constrained locations, aiming to satisfy specific requirements of the industry, such as average energy consumption and average network reliability. This constrained relay node deployment problem (CRNDP) is known as NP-hard optimization problem in the literature. We consider addressing this multi-objective (MO) optimization problem with an improved Artificial Bee Colony (ABC) algorithm with a linear local search (MOABCLLS), which is an extension of an improved ABC and applies two strategies of MO optimization. In order to verify the effectiveness of the MOABCLLS, two versions of MO ABC, two additional standard genetic algorithms, NSGA-II and SPEA2, and two different MO trajectory algorithms are included for comparison. We employ these metaheuristics on a test data set obtained from the literature. For an in-depth analysis of the behavior of the MOABCLLS compared to traditional methodologies, a statistical procedure is utilized to analyze the results. After studying the results, it is concluded that constrained relay node deployment using the MOABCLLS outperforms the performance of the other algorithms, based on two MO quality metrics: hypervolume and coverage of two sets.

An Adaptive Fault-tolerant Control Method for Robot Manipulators

Wenjie Zhang,Xiaohui Yang,Zhenghong Xu,Wei Zhang,Li Yang,Xiaoping Liu 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.12

This paper presents a new method for adaptive continuous nonsingular fast terminal sliding mode control (ACNFTSMC) based on a novel structure-improved extended state observer (SIESO) for fault-tolerant control (FTC). In response to the initial peaking phenomenon in traditional ESO (TESO), which can severely degrade the accuracy and stability of the control system, The SIESO is designed to replace TESO to estimate the lump disturbances/faults. Besides, to address the problem of the unknown estimation error of ESO, an adaptive technique is applied to compensate for the observation error in real-time. To guarantee fast convergence and chattering-free, the CNFTSMC method is employed. Afterward, the stability and rapid convergence of the control system is demonstrated using Lyapunov theory. Finally, the simulation results verify the superiority of the proposed control strategy compared to the other existing advanced control techniques.

Flexural Performance of Steel Reinforced ECC-Concrete Composite Beams Subjected to Freeze-Thaw Cycles

Wenjie Ge,Ashraf F. Ashour,Weigang Lu,Dafu Cao 한국콘크리트학회 2020 International Journal of Concrete Structures and M Vol.14 No.2

Experimental and theoretical investigations on the flexural performance of steel reinforced ECC-concrete composite beams subjected to freeze-thaw cycles are presented in this paper. Four groups of reinforced composite beams with different ECC height replacement ratios subject to 0, 50, 100 and 150 cycles of freeze-thaw were physically tested to failure. Experimental results show that the bending capacity decreases with the increase of freeze-thaw cycles regardless of ECC height replacement ratios. However, the ultimate moment, stiffness and durability of ECC specimens and ECC-concrete composite specimens are greater than those of traditional concrete specimens, owing to the excellent tensile performance of ECC materials. With the increase of ECC height, the crack width and average crack spacing gradually decrease. According to materials’ constitutive models, compatibility and equilibrium conditions, three failure modes with two boundary failure conditions are proposed. Simplified formulas for the moment capacity are also developed. The results predicted by the simplified formulas show good agreement with the experimental moment capacity and failure modes. A parametric analysis is conducted to study the influence of strength and height of ECC, amount of reinforcement, concrete strength and cycles of freeze-thaw on moment capacity and curvature ductility of ECC-concrete composite beams.

MXene/PVA Fiber-based Supercapacitor with Stretchability for Wearable Energy Storage

Wenjie Yu,Yu Li,Binjie Xin,Zan Lu 한국섬유공학회 2022 Fibers and polymers Vol.23 No.11

MXene fiber-based supercapacitor exhibits a great potential for wearable energy storage devices with a largesurface area, good conductive, and higher power density. However, MXene flakes are difficult to spin independently due tothe non-liquid crystal structure. In the report, we fabricated freestanding MXene/PVA fibers with good stretchability andimpressive electrochemical properties for fiber-based supercapacitors by a simple wet spinning method. The tensile strengthof the MXene/PVA fibers is significantly enhanced compared with pure PVA fibers for the concentration of MXene foraround 5-20 wt.%. The elongation of the MXene/PVA fiber can reach 48 %, even if the MXene concentration increase to25 wt.%. MXene flakes also increase the thermal stability of hybrid fibers compared with pure PVA fibers. MXene/PVAfiber-based supercapacitors act as efficient energy storage devices, which display high gravimetric capacitance (119.3 F g-1),and areal capacitance (130.9 mF cm-2), strong durability in multiple mechanical states, and steady electrochemical behaviorafter stretching.

VARIABLE CROSS-SECTION RECTANGULAR BEAM AND SENSITIVITY ANALYSIS FOR LIGHTWEIGHT DESIGN OF BUS FRAME

Wenjie Zuo,Jiaxin Fang,Minghui Zhong,Guikai Guo 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.6

Timoshenko beam element of variable cross-section rectangular tube is developed and applied in the lightweight design of bus frame in this paper. Firstly, the finite element formulations of variable cross-section beam (VCB) are derived under the loadsteps of axial deformation, torsional deformation and bending deformation. Secondly, bending deformation experiment and its detailed shell finite element model (FEM) simulation of variable cross-section rectangular tube were conducted; and the proposed VCB, detailed shell FEM and experimental results can be highly consistent. Thirdly, VCBs are used to substitute for parts of the uniform ones in a bus frame. An innovatively lightweight bus frame is obtained and all the performance responses are improved simultaneously. Finally, rollover analysis further shows the advantage of variable cross-section bus frame in crashworthiness design.

Improved Ethanol Production in Jerusalem Artichoke Tubers by Overexpression of Inulinase Gene in Kluyveromyces marxianus

WenJie Yuan,Xinqing Zhao,Li-Jie Chen,Feng-Wu Bai 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4

Ethanol production from Jerusalem artichoke tubers through a consolidated bioprocessing (CBP) strategy using the inulinase-producing yeast Kluyveromyces marxianus is an economical and competitive than that from a grainbased feedstock. However, poor inulinase production under ethanol fermentation conditions significantly prolongs the fermentation time and compromises ethanol productivity. Improvement of inulinase activity appears to be promising for increasing ethanol production from Jerusalem artichoke tubers by CBP. In the present study, expression of the inulinase gene INU with its own promoter in K. marxianus (K/INU2) was explored using the integrative cassette. Overexpression of INU was explored using chromosome integration via the HO locus of the yeast. Inulinase activity and ethanol were determined from inulin and Jerusalem artichoke tubers under fed-batch operation. Inulinase activity was 114.9 U/mL under aerobic conditions for K/INU2, compared with 52.3 U/mL produced by the wild type strain. Importantly, inulinase production was enhanced in K/INU2 under ethanol fermentation conditions. When using 230 g/L inulin and 220 g/L Jerusalem artichoke tubers as substrates, inulinase activities of 3.7 and 6.8 U/mL,respectively, were measured using K/INU2, comparing favorably with 2.4 and 3.1 U/mL, respectively, using the wide type strain. Ethanol concentration and productivity for inulin were improved by the recombinant yeast to 96.2 g/L and 1.34 g/L/h, respectively, vs 93.7 g/L and 1.12 g/L/h,respectively, by the wild type strain. Ethanol concentration and productivity improvements for Jerusalem artichoke tubers were 69 g/L and 1.44 g/L/h, respectively, from the recombinant strain vs 62 g/L and 1.29 g/L/h, respectively,from the wild type strain.

The application of a regularization method to the estimation of geometric errors of a three-axis machine tool using a double ball bar

Wenjie Tian,Guang Yang,Lina Wang,Fuwen Yin,Weiguo Gao 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.10

Geometric accuracy is crucially important for machine tools. Identification of geometric errors, especially position-dependent geometric errors, is still a challenging issue. This paper presents a systematic and fast approach to identify the geometric error components of a precision machine tool using double ball bar (DBB). The approach can be implemented in three steps: (1) polynomial based error modeling that relates the DBB radius error directly to the geometric error parameters of machine tool; (2) spatial measurement trajectory planning with a single installation of DBB in order to avoid producing extra setup errors; (3) error identification with regularization method that can solve the ill-posed identification problem effectively. Simulations and experiments show the accuracy and effectiveness of the proposed identification approach. The results of the DBB test show that, utilizing the proposed identification method, the roundness errors of the three circular paths in xy-, yz- and xz-plane are reduced from 27.3 μm, 20.7 μm and 24.1 μm to 9.2 μm, 12.3 μm and 7.8 μm, respectively, with error compensation.