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        • KCI등재SCOPUS

          Quantification of SPS mRNA Expression in Banana Fruit Ripened under Different Conditions Using Real-time RT-PCR

          Wen,Li,Xue-ping,Li,Yuan-zhi,Shao,Jiang-hui,Xie,Wei-xin,Chen,Zhi-qiang,Jin 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.6

          Real-time reverse transcriptase (RT)-PCR using SYBR GreenI was applied to quantify sucrose phosphate synthase (SPS) mRNA expression at different storage days during the natural ripening (with no treatment), inhibited ripening with ethylene absorbent and accelerated ripening by treatment with propylene of banana fruit. The results showed that expression level of SPS mRNA were significantly different between 3 kinds of ripening fruit. Expression of SPS mRNA in fruit treated with propylene was dramatically promoted, the peak of SPS mRNA expression in these fruits appeared 16 days earlier than that in fruit with natural ripening. On the contrary, when fruit applied with ethylene absorbent, SPS mRNA expression was remarkably inhibited, the amount of SPS mRNA expression were significantly lower than that in naturally ripened fruit. These results indicated that the expression level of SPS mRNA of banana fruit can be impacted by regulating ethylene evolution, and that close relationships may exist between SPS mRNA expression and SPS activity, fruit softening, respiration rate, and ethylene production during ripening of banana fruit.

        • KCI등재SCIESCOPUS

          Simultaneous Information and Power Transfer for Multi-antenna Primary-Secondary Cooperation in Cognitive Radio Networks

          Zhi,Hui,Liu,Wen,Jun,Xu,Sheng,Yu,Li,Cheng,Zhi,Long,Jia,Ru,Lin 한국전자통신연구원 2016 ETRI Journal Vol.38 No.5

          In this paper, cognitive radio and simultaneous wireless information and power transfer (SWIPT) are effectively combined to design a spectrum-efficient and energy-efficient transmission paradigm. Specifically, a novel SWIPT-based primary-secondary cooperation model is proposed to increase the transmission rate of energy/spectrum constrained users. In the proposed model, a multi-antenna secondary user conducts simultaneous energy harvesting and information forwarding by means of power splitting (PS), and tries to maximize its own transmission rate under the premise of successfully assisting the data delivery of the primary user. After the problem formulation, joint power splitting and beamforming optimization algorithms for decode-and-forward and amplify-and-forward modes are presented, in which we obtain the optimal PS factor and beamforming vectors using a golden search method and dual methods. Simulation results show that the proposed SWIPTbased primary-secondary cooperation schemes can obtain a much higher level of performance than that of non-SWIPT cooperation and non-cooperation schemes.

        • KCI등재SCOPUS

          Random vibration analysis of train-slab track-bridge coupling system under earthquakes

          Zhi-ping,Zeng,Xian-feng,He,Yan-gang,Zhao,Zhi-wu,Yu,Ling-kun,Chen,Wen-tao,Xu,Ping,Lou 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.54 No.5

          This study aimed to investigate the random vibration characteristic of train-slab track-bridge interaction system subjected to both track irregularities and earthquakes by use of pseudo-excitation method (PEM). Each vehicle subsystem was modeled by multibody dynamics. A three-dimensional rail-slabgirder-pier finite element model was created to simulate slab track and bridge subsystem. The equations of motion for the entire system were established based on the constraint condition of no jump between wheel and rail. The random load vectors of equations of motion were formulated by transforming track irregularities and seismic accelerations into a series of deterministic pseudo-excitations according to their respective power spectral density (PSD) functions by means of PEM. The time-dependent PSDs of random vibration responses of the system were obtained by step-by-step integration method, and the corresponding extreme values were estimated based on the first-passage failure criterion. As a case study, an ICE3 high-speed train passing a fifteen-span simply supported girder bridge simultaneously excited by track irregularities and earthquakes is presented. The evaluated extreme values and the PSD characteristic of the random vibration responses of bridge and train are analyzed, and the influences of train speed and track irregularities (without earthquakes) on the random vibration characteristic of bridge and train are discussed.

        • Quality of Life for Patients with Esophageal/Gastric Cardia Precursor Lesions or Cancer: A One-year Prospective Study

          Wen,,Ying,Pan,,Xiong-Fei,Huang,,Wen-Zhi,Zhao,,Zhi-Mei,Wei,,Wen-Qiang,Chen,,Feng,Lan,,Hui,Huang,,He,Yang,,Chun-Xia,Qiao,,You-Lin Asian Pacific Organization for Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1

          Background: The current study examined health-related quality of life (QoL) for patients with esophageal/gastric cardia precursor lesions or cancer before and after treatment to facilitate improved prevention and treatment. Materials and Methods: Patients with different stages of esophageal/gastric cardia lesions completed two QoL questionnaires, EORTC QLQ-C30 and supplemental QLQ-OES 18, before primary treatment, and at 1, 6 and 12 months after treatment. Results: Fifty-nine patients with precursor lesions, 57 with early stage cancer, and 43 with advanced cancer responded to our survey. Patients with precursor lesions or early stage cancer reported better QoL overall than those with advanced cancer before treatment (p<0.01). Global QoL scores before treatment and at 1 month after treatment were $71{\pm}9$ versus $69{\pm}9$ (p>0.01), $71{\pm}8$ versus $61{\pm}11$ (p<0.01), $67{\pm}11$ versus $62{\pm}9$ (p<0.01) for three stages of lesions. At 6 months after treatment, some QoL measures recovered gradually in precursor lesion and early cancer patients, while some continuously deteriorated in advanced cancer patients. At 12 months, all QoL scores were comparable to baseline for patients with precursor lesions (p>0.01), while global QoL, social, pain, and insomnia scores for early stage and advanced cancer were inferior to corresponding baseline levels (difference between means>5, p<0.01). At this time point, compared with patients with early stage cancer, those with advanced cancer showed worse QoL with all function and most symptom measures (p<0.01). Conclusions: Patients with precursor lesions or early stage esophageal/gastric cardia cancer show better QoL than those with advanced cancer. This indicates that screening, early diagnosis and treatment may improve the QoL for esophageal/gastric cardia cancer patients. Target intervention and counseling should be given by health care providers during treatment and follow-up to facilitate QoL improvement.

        • ADPRT Val762Ala and XRCC1 Arg194Trp Polymorphisms and Risk of Gastric Cancer in Sichuan of China

          Wen,,Yuan-Yuan,Pan,,Xiong-Fei,Loh,,Marie,Tian,,Zhi,Yang,,Shu-Juan,Lv,,Si-Han,Huang,,Wen-Zhi,Huang,,He,Xie,,Yao,Soong,,Richie,Yang,,Chun-Xia Asian Pacific Organization for Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

          Objective: Gastric cancer remains a major health problem in China. We hypothesized that XRCC1 Arg194Trp and ADPRT Val762Ala may be associated with risk. Methods: We designed a multicenter 1:1 matched case-control study of 307 pairs of gastric cancers and controls between October 2010 and August 2011. XRCC1 Arg194Trp and ADPRT Val762Ala were sequenced, and demographic data as well as lifestyle factors were collected using a self-designed questionnaire. Results: Individuals carrying XRCC1 Trp/Trp or Arg/Trp variant genotype had a significantly increased risk of gastric cancer (OR, 1.718; 95% CI, 1.190-2.479), while the OR for ADPRT Val762Ala variant genotype (Ala/Ala or Val/Ala) was 1.175 (95% CI, 0.796-1.737). No gene-gene or gene-environment interactions were found. In addition, family history of cancer and drinkers proportion were higher among cases than among controls (P<0.05). Conclusions: XRCC1 194 Arg/Trp or Trp/Trp genotype, family history of cancer, and drinking are suspected risk factors of gastric cancer from our study. Our findings may offer insight into further similar large gene-environment and gene-gene studies in this region.

        • KCI등재SCOPUS

          An Efficient Multi-PCA Based On-line Monitoring Scheme for Multi-Stages Imperial Smelting Process

          Zhi-kun,Hu,Zhi-wen,Chen,Wei-Hua,Gui,Chun-hua,Yang,Lin-zi,Yin,Xiao-qi,Peng 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.2

          Imperial Smelting Process (ISP) is one of the main methods for Zinc and Lead smelting. In this paper, we firstly focus on its unique characteristics that ISP consists of many feeding cycles repeated in a similar way, and the normal process changes and multiple operating conditions would be frequently found in the process which would always lead to false alarms. To this end, we classify each feeding cycles of ISP into initial stage, transition stage, and steady stage, and use standard PCA, first order perturbation (FOP) based recursive dynamic PCA and multi-mode approach to deal with them respectively. The monitoring results using real data show that the proposed scheme can be able to eliminate false alarms and detect faults efficiently.

        • SCIESCOPUSKCI등재

          Immunization of BALB/c mice with <i>Brucella abortus </i>2308Δ<i>wbkA</i> confers protection against wild-type infection

          Zhi-qiang,Li,Dan,Gui,Zhi-hua,Sun,Jun-bo,Zhang,Wen-zhi,Zhang,Hui,Zhang,Fei,Guo,Chuang-fu,Chen 대한수의학회 2015 JOURNAL OF VETERINARY SCIENCE Vol.16 No.4

          '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

          Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the <i>Brucella (B.) abortus</i> 2380 <i>wbkA</i> mutant (2308Δ<i>wbkA</i>) and evaluated its virulence. The survival of 2308Δ<i>wbkA</i> was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The <i>wbkA </i>mutant elicited an anti-<i>Brucella</i>-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308Δ<i>wbkA</i> could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The <i>WbkA </i>antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308Δ<i>wbkA</i> is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.

        • KCI등재SCOPUS

          Crack tip shielding and anti-shielding effects of parallel cracks for a superconductor slab under an electromagnetic force

          Zhi-Wen,Gao,이강용,You-He,Zhou 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.2

          In this letter, the shielding or anti-shielding effect is firstly applied to obtain the behavior of two parallel cracks in a two-dimensional type-II superconducting under electromagnetic force. Fracture analysis is performed by the finite element method and the magnetic behavior of superconductor is described by the critical state Bean model. The stress intensity factors at the crack tips can be obtained and discussed for decreasing field after zero-field cooling. The shielding or anti-shielding effect at the crack tips depend on the distance between two parallel cracks and the crack length. The results indicate that the shielding effects of the two parallel cracks increase when the distance between the two parallel cracks decreases. It can be also obtained that the superconductors with shorter cracks has more remarkable shielding effect than those with longer cracks.

        • KCI등재SCOPUS

          CFD based simulations of flutter characteristics of ideal thin plates with and without central slot

          Zhi-wen,Zhu,Zheng-qing,Chen,Ming,Gu 한국풍공학회 2009 Wind and Structures, An International Journal (WAS Vol.12 No.1

          In this paper, the airflow around an ideal thin plate (hereafter referred to as ITP) with various ratios of central slot is simulated by using the finite-difference-method (FDM)-based Arbitrary-Lagrangian-Eulerian descriptions for the rigid oscillating body. The numerical procedure employs the second-order projection scheme to decouple the governing equations, and the multigrid algorithm with three levels to improve the computational efficiency in evaluating of the pressure equation. The present CFD method is validated through comparing the computed flutter derivatives of the ITP without slot to Theodorsen analytical solutions. Then, the unsteady aerodynamics of the ITP with and without central slot is investigated. It is found that even a smaller ratio of central slot of the ITP has notable effects on pressure distributions of the downstream section, and the pressure distributions on the downstream section will further be significantly affected by the slot ratio and the reduced wind speeds. Continuous increase of with the increase of central slot may be the key feature of the slotted ITP. Finally, flutter analyses based on the flutter derivatives of the slotted ITP are performed, and moreover, flutter instabilities of a scaled sectional model of a twin-deck bridge with various ratios of deck slot are investigated. The results confirm that the central slot is effective to improve bridge flutter stabilities, and that the flutter critical wind speeds increase with the increase of slot ratio.

        • KCI등재SCOPUS

          Remarkable impact of amino acids on ginsenoside transformation from fresh ginseng to red ginseng

          Zhi,Liu,Xin,Wen,Chong-Zhi,Wang,Wei,Li,Wei-Hua,Huang,Juan,Xia,Chang-Chun,Ruan,Chun-Su,Yuan 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.3

          Background: Amino acids are one of the major constituents in Panax ginseng, including neutral aminoacid, acidic amino acid, and basic amino acid. However, whether these amino acids play a role in ginsenosideconversion during the steaming process has not yet been elucidated. Methods: In the present study, to elucidate the role of amino acids in ginsenoside transformation fromfresh ginseng to red ginseng, an amino acids impregnation pretreatment was applied during thesteaming process at 120 C. Acidic glutamic acid and basic arginine were used for the acid impregnationtreatment during the root steaming. The ginsenosides contents, pH, browning intensity, and free aminoacids contents in untreated and amino acidetreated P. ginseng samples were determined. Results: After 2 h of steaming, the concentration of less polar ginsenosides in glutamic acidetreatedP. ginseng was significantly higher than that in untreated P. ginseng during the steaming process. However,the less polar ginsenosides in arginine-treated P. ginseng increased slightly. Meanwhile, free aminoacids contents in fresh P. ginseng, glutamic acid-treated P. ginseng, and arginine-treated P. ginsengsignificantly decreased during steaming from 0 to 2h. The pH also decreased in P. ginseng samples at hightemperatures. The pH decrease in red ginseng was closely related to the decrease in basic amino acidslevels during the steaming process. Conclusion: Amino acids can remarkably affect the acidity of P. ginseng sample by altering the pH value. Theywere the main influential factors for the ginsenoside transformation. These results are useful in elucidatingwhy andhowsteaming induces the structural change of ginsenoside in P. ginseng and also provides an effectiveand green approach to regulate the ginsenoside conversion using amino acids during the steaming process.

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