Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

Observation of multi-channel non-local transport in J-TEXT plasmas

Shi, Yuejiang,Chen, Zhongyong,Yang, Zhoujun,Shi, Peng,Zhao, Kaijun,Diamond, Patrick H.,Kwon, JaeMin,Yan, Wei,Zhou, Hao,Pan, Xiaoming,Cheng, Zhifeng,Chen, Zhiping,Yang, SeongMoo,Zhang, Chi,Li, Da,Dong, IOP 2018 Nuclear fusion. Fusion nucléaire. &n.Illiga Vol.58 No.4

<P>In cold pulse experiments in J-TEXT, not only are rapid electron temperature increases in the core observed, but also steep rises in the inner density are found. Moreover, some evidence of acceleration of the core toroidal rotation is also observed during the non-local transport process of electron temperature. These new findings of cold pulse experiments in J-TEXT suggest that turbulence spreading is a possible mechanism for the non-local transport dynamics.</P>

Towards Energy-aware Web Service Composition

Wei Wang,Suichu Zhai,XiaoXia Shi 보안공학연구지원센터 2014 International Journal of u- and e- Service, Scienc Vol.7 No.6

In recent years, the Web service composition technology has developed rapidly, as a result, the energy consumption problem is also increasingly prominent. Faced with this situation, the paper firstly gives out the power model of single service; then derives the model of service composition according to its characteristics, and gives out the corresponding QoS constraints at the same time. After finishing the model, the paper talks about the allocation of feasible path, which is one of the problem in model calculation. The author introduces a graph-plan based searching algorithm, and uses the concept of hierarchical search to solve the problem. At last, the experimental results show that compared with traditional methods, the approach in this paper can not only satisfy the user requests with QoS restricted, but also reduce the energy consumption of service composition effectively.

Ileus tube placement combined with laparoscopy in the treatment of refractory small bowel obstruction: Case series and literature review

Wei-Hai Shi,Hao-Qiang Chao,Nian-Yuan Ye,Hong-Jian Shi 소화기인터벤션의학회 2019 International journal of gastrointestinal interven Vol.8 No.1

Post-surgery small bowel obstruction (SBO) has substantial morbidity and mortality. Even with refinement of surgical technique, SBO still remains challenging with the most common causes of acute hospital admission. Most SBOs can be managed with conservative treatment. However, the pal-liative methods may fail to relieve the symptoms in some clinical settings. Currently, laparotomy is the standard of care for difficult SBO. And lapa-roscopy offers an alternative approach for operative intervention in SBO and was first described by Bastug et al in 1991. Emerging literature dem-onstrated that laparoscopic surgery for SBO is less invasive, resulting in a shorter hospital stay than open surgery. And most recurrent adhesive SBO can be managed with ileus tube placement. This case series is to evaluate the efficacy of transnasal ileus tube placement combined with laparoscopic surgery in the treatment of consecutive 5 cases of refractory SBO post oncologic gastrointestinal surgery.

A 60GHz Wireless Cooperative Communication System Based on Switching Beamforming

( Wei Shi ),( Jingjing Wang ),( Yun Liu ),( Qiuna Niu ),( Hao Zhang ),( Chunlei Wu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.4

The challenge of penetrating obstacles along with impact from weak multipath effects makes 60GHz signal very difficult to be transmitted in non-line of sight (NLOS) channel. So 60GHz system is vulnerable to obstructions and thus likely results in link interruption. While the application of cooperative technology to solve link blockage problemin 60GHz system should consider the characteristic of directional transmission for 60GHz signal. Therefore in this paper a system is proposed to solve the link blockage problem in 60GHz NLOS communication environment based on the concept of cooperation and also the beamforming technology, which is the basis of directional transmission for 60GHz communication system. The process of anti-blockage solution with cooperative communication is presented in detail, and the fast switching and recovery schemes are well designed. The theoretical values of symbol error rate (SER) using decode and forward (DF) cooperation and amplify and forward (AF) cooperation are presented respectively when the common channel interference exists. Simulation results demonstrate that the performance based on DF cooperation is better than the performance based on AF cooperation when directional transmission is used.

Parametric study of a drivetrain in horizontal wind turbine using torsional model

Wei Shi,Hyunchul Park(박현철),Changwan Kim(김창완),Chinwha Chung(정진화),Youngchan Kim(김영찬) 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.5-2

Chinese Patients with Gastric Cancer Need Targeted Adjuvant Chemotherapy Schemes

Shi, Wen-Tao,Wei, Lei,Xiang, Jin,Su, Ke,Ding, Qiong,Tang, Meng-Jie,Li, Ji-Qiang,Guo, Yi,Wang, Pu,Zhang, Jing-Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Background: Gastric cancer (GC) is one of the most common cancers in China. Adjuvant chemotherapy (AC) is a routine auxiliary treatment for GC recommended by the guidelines issued in 2011 by the Ministry of Health of the People's Republic of China, but the relevant credible consequences in China have been insufficient because of China's late start and ethical concerns. Methods: A series of databases, including Cochrane Library, MEDLINE, EMBASE, the Chinese database of the National Knowledge Infrastructure and the VIP database, were searched by 2 reviewers independently for studies investigating AC for GC through March 2012. The retrieved literature was screened according to the eligibility criteria. Results: A total of 35 randomized control trials (RCTs) were subjected to the final analysis, including 4,043 patients in treatment group and 3,884 in the control group, as well as 4 clinical-control trials (CCTs), which accessed the final analysis with 238 and 252 patients, respectively. AC reduced the risk of death as a protective treatment with statistical significance (HR=0.91, 95%CI: [0.85, 0.97], P=0.002), and it seemed more effective for Asian than non-Asian patients. The effects of AC were not influenced by the starting time (P>0.05). D2 lymphadenectomy-based chemotherapy was effective (HR=0.89, 95%CI: [0.80, 0.99], P=0.04). Oral S-1 40 mg/m2 after D2 lymphadenectomy might be a better choice for Asians with advanced GC and might result in a greater reduction of adverse events than in non-Asian patients. GRADE quality assessment determined that the strength of the evidence from foreign studies from Europe, the United States and Asian countries other than China was high, while it was moderate for Chinese studies. Conclusion: AC was effective or even curative in Chinese patients in general, although it is still necessary to optimize a targeted AC scheme for Chinese patients with GC.

ON A CONJECTURE OF GRAHAM

Wei, Shi Ming,Jun, Young-Bae The Youngnam Mathematical Society Korea 1994 East Asian mathematical journal Vol.10 No.2

Homoisoflavanones from Polygonatum odoratum Rhizomes Inhibit Advanced Glycation End Product Formation

Wei Dong,Hai Bo Shi,Heng Ma,Yan Bo Miao,Tong Jun Liu,Wei Wang 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.5

Protein glycation inhibitors from Polygonatum odoratum rhizomes were investigated using a bioassay-guided procedure to characterize active compounds for preventing and treating diabetic complications. The EtOH extract and soluble fractions were evaluated using an in vivo model of renal advanced glycation end-product (AGE) accumulation in streptozotocin-induced diabetic rats and an in vitro bovine serum albumin-glucose assay. Three homoisoflavanones 3-(4’-hydroxybenzyl)-5,7-dihydroxy-6-methyl-8-methoxychroman-4-one (1), 3-(4’-hydroxybenzyl)-5,7-dihydroxy-6,8-dimethylchroman-4-one (2), and 3-(4’-methoxybenzyl)-5,7-dihydroxy-6-methyl-8-methoxychroman-4-one (3), isolated from the active CHCl3-soluble fraction of the EtOH extract, were subjected to in vitro bioassays to evaluate their inhibitory activities against AGE formation. All the isolates inhibited AGE formation more effectively than the positive control, aminoguanidine. These results indicate that pending further study these compounds could be used as novel natural product drug for mitigating diabetic complications.

Characteristics of Carbohydrate Assimilation and Distribution in Walnut (Juglans regia L.)

Shi-Wei Wang,Cun-De Pan,Cui-Fang Zhang,Hong Chen 한국원예학회 2021 원예과학기술지 Vol.39 No.2

Based on <SUP>13</SUP>C isotope tracer technology, we investigated the <SUP>13</SUP>C abundance (δ<SUP>13</SUP>C), <SUP>13</SUP>C content(<SUP>13</SUP>Camount), and new fixed <SUP>13</SUP>C percentage (<SUP>13</SUP>CPCT) in different organs and different metabolic components in girdled fruit-bearing shoots at different stages of fruit growth and development in walnut. Our aim was to analyze the dynamic characteristics of carbohydrate assimilation, transportation, and utilization in different stages of walnut fruit growth and development. The results showed that (1) at 0 h after the feeding, δ<SUP>13</SUP>C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT in sucrose from the leaves were the highest and positively correlated with net photosynthetic rate of leaves (p < 0.05); (2) at 24 h after the feeding, δ13C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT in sucrose from the carpopodium reached their peak values; (3) δ<SUP>13</SUP>C,<SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT in sucrose from the peel were lower than those of hexose at 48 h after feeding in the fast-growing stage, but they all reached maximum levels in peel sucrose at 48 h after feeding in the oil conversion stage; and (4) the highest δ<SUP>13</SUP>C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT were recorded in all carbohydrates from the seed kernel 48 h after feeding, among which the highest δ<SUP>13</SUP>C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT were recorded in sucrose. These results suggested that sucrose was the main form of carbohydrate assimilation and transportation in walnut fruit, and large amounts of sucrose are accumulated in the peel and in the kernel, where it is also the base for the transformation of oil.