Hypoxia-induced PLOD2 regulates invasion and epithelial-mesenchymal transition in endometrial carcinoma cells

Junhui Wan,Junli Qin,Qinyue Cao,Ping Hu,Chunmei Zhong,Chunhua Tu 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2) was induced in hypoxia and participated in cancer development. However, the role of PLOD2 in endometrial carcinoma remains unclear. Objective To explore the influences and regulation mechanism of PLOD2 in endometrial carcinoma under hypoxic condition. Methods The small interfering RNA (siRNA) targeting to PLOD2 and pcDNA3.1-PLPD2 were transfected to endometrial carcinoma cells to alter PLOD2 expression. Cell proliferation ability was determined by colony formation assay. Wound healing assay used to detect cell migration ability. Transwell invasion assay was used to detect cell invasion ability. Results PLOD2 and Hypoxia-inducible factor-1α (HIF-1α) were induced by hypoxia. Down-regulation of PLOD2 did not affect endometrial carcinoma cell proliferation ability, while inhibited cell migration, invasion under hypoxic condition. Besides, down-regulation of PLOD2 increased the levels of γ-catenin and E-cadherin and decreased levels of Fibronectin and Snail under hypoxic condition. Down-regulation of PLOD2 also inactivated Src and phosphoinositide 3-kinase (PI3K)/ protein kinase B (Akt) signaling under hypoxic condition. The promoting effects of PLOD2 overexpression on migration, invasion and epithelial-mesenchymal transition (EMT) of endometrial carcinoma cells were reversed by Akt inhibitor (MK2206) under hypoxic condition. Conclusion PLOD2 expression was increased in endometrial carcinoma cells under hypoxic condition. PLOD2 modulated migration, invasion, and EMT of endometrial carcinoma cells via PI3K/Akt signaling. PLOD2 may be a potential therapeutic target for endometrial carcinoma.

Identification of a Novel Human Zinc Finger Gene, ZNF438, with Transcription Inhibition Activity

Zhong, Zhaomin,Wan, Bo,Qiu, Yun,Ni, Jun,Tang, Wenwen,Chen, Xinya,Yang, Yun,Shen, Suqin,Wang, Ying,Bai, Meirong,Lang, Qingyu,Yu, Long Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.4

There were many different families of zinc finger proteins that contained multiple cysteine and/or histidine residues and used zinc to stabilize their folds. The classical C2H2 zinc finger proteins were the founding members of this superfamily and were among the most abundant proteins in eukaryotic genomes. C2H2 proteins typically contained several C2H2 fingers that made tandem contacts along the DNA. Here we reported a novel C2H2 type zinc finger gene, ZNF438, which encoded 828 amino acids that formed five zinc finger domains. Bioinformatics analysis revealed that the ZNF438 was mapped to human chromosome 10p11.2 and shared 62% identity with rat and mouse homologues. RT-PCR analysis indicated that it was ubiquitously expressed in 18 human adult tissues. With immunofluorescence assay, it was shown that the exogenous Flag-tagged ZNF438 was located in nucleus of COS-7 cells. To further explore the function of ZNF438, we examined the transcriptional activity of ZNF438 protein by transfecting recombinant pM-ZNF438 into mammalian cells. The subsequent analysis based on the duel luciferase assay system showed that ZNF438 was a transcriptional repressor.

Single Nucleotide Polymorphisms in the u-PA Gene are Related to Susceptibility to Oral Tongue Squamous Cell Carcinoma in the Northern Chinese Han Population

Zhong, Feng,Yang, Xue-Cai,Bu, Ling-Xue,Li, Ning-Yi,Chen, Wan-Tao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Aim: The purpose of this study was to determine whether susceptibility to oral tongue squamous cell carcinoma (OSCC) is related to polymorphisms in the u-PA gene. Methods: We examined the rs2227564 C/T and rs2227562 G/A single nucleotide polymorphisms (SNPs) in 196 OSCC patients and 201 age- and gender-matched controls via direct sequencing and PCR-RFLP methods. Results: Significant differences were found in allelic and genotypic distributions of the rs2227564 and rs2227562 loci when comparing cases and controls. In addition, logistic analyses indicated that the rs2227564 C/T genotype was related to a 1.52-fold increased risk of developing OSCC (adjusted OR=1.521, 95%CI: 1.144~2.022, P=0.004). Linkage disequilibrium analysis was conducted and no association between the two loci was found (D'=0.031, $r^2$=0.000). Conclusions: Our findings provide evidence that the rs2227564 C/T SNP in the u-PA gene is associated with the development of OSCC.

Observation of the Trapping of Runaway Electrons into Local Magnetic Mirrors during Slide-away Discharges in the HT-7 Tokamak

Zhong Yong Chen,Bao Nian Wan,Yuejiang Shi 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.2

The trapping of runaway electrons into local magnetic mirrors in a toroidal magnetic field has been observed in slide away discharges in the HT-7 tokamak. When the discharges went into the slide-away region with Parail-Pogutse instabilities, strong oscillations in the hard X-ray flux and in the electron cyclotron emission are observed. The runaway electrons are trapped into the local magnetic mirrors when they are pitch angle scattered by the anomalous Doppler resonance effect.

중국 오디오 스트리밍 플랫폼의 산업 진화와 발전전략 연구 : ‘히말라야(喜馬拉雅)’ 플랫폼을 중심으로

중메이(Zhong Mei),한창완(Chang-Wan Han) 한국애니메이션학회 2022 애니메이션연구 Vol.18 No.1

스마트폰 등 스마트 기기와 커넥티드 카 등 스마트 디바이스의 보급으로 오디오 스트리밍 서비스가 더욱 다양한 상황에서 활용이 가능해지고 있다. 오디오 스트리밍 플랫폼에서 스마트 스피커, 이어폰 등 하드웨어 제품과 자동차용 오디오 플랫폼을 출시하면서 오디오 스트리밍 산업의 스마트화 발전 추세를 보여주고 있다. 중국 오디오 스트리밍 산업은 기술, 자본, 사용자 및 콘텐츠의 상호작용을 통해 지속적인 발전을 이루고 있는 가운데 오디오 콘텐츠는 일상생활의 다양한 상황에서 활용되고 있다. 또한 각 플랫폼의 성장과 경쟁을 바탕으로 오늘날 오디오 스트리밍 산업의 수익 모델은 점차 차별화를 이루고 있다. 이에 따라 본 연구는 마셜 매클루언(Marshall McLuhan)의 청각 공간과 데이비드 하비(David Harvey)의 공간의 압축 등 측면에서 오디오 스트리밍 산업의 발전 현황과 사용자의 경험 변화를 분석함으로써 이와 같은 변화에 대응하기 위한 오디오 스트리밍 플랫폼의 콘텐츠 생산 모델과 차별화된 수익 모델을 분석하였다. With the spread of smart devices such as smartphones and connected cars, audio streaming services are becoming available in more diverse situations. With the launch of hardware products such as smart speakers and earphones on audio streaming platforms and audio platforms for automobiles, the audio streaming industry is showing a trend of smart development. While the Chinese audio streaming industry is continuously developing through the interaction of technology, capital, users, and content, audio content is being used in various situations in everyday life. In addition, based on the growth and competition of each platform, the revenue model of the audio streaming industry today is gradually differentiating. Accordingly, this study analyzed the development status of the audio streaming industry and changes in user experience in terms of McLuhan’s auditory space and David Harvey’s space compression, and analyzed the differentiated profit model from the content production model of the audio streaming platform to cope with these changes.

강편 빌레트의 건식 자분 탐상

이의완,김구화,임종수 韓國非破壞檢査學會 1996 한국비파괴검사학회지 Vol.16 No.3

본 연구는 강편 빌레트의 표면 결함을 검출하기 위한 건식 자분 탐상에 관한 것으로 자분 탐상능을 대상체에 흘리는 자화 전류, 대상체의 온도, 자분의 총 분사량 등에 대하여 평가하였다. 선재 제품의 등급에 따라 필요로 하는 몇까지 강종을 선택하여 강종별 자기적 특성을 평가하였으며, 이를 입력자료로 하여 유한 요소법에 의한 자기 해석을 행하였고, 그 결과를 직류 자화 전류에 의한 누설 자속 측정 결과와 비교 분석하였다. 교류 자화 전류에 의한 건식 자분 탐상능을 직류 자화 전류에 의한 탐상능과 비교하여 강종 및 자화 전류의 유형에 따른 자화 전류치를 결정하였다. 직류 자화 전류에 의한 자분 탐상 결과를 유한 요소법에 의한 계산과 비교하였고, 빌레트의 표면과 표면 결함 부위에서 측정한 누설 자속으로 비교 결과를 평가하였다. 각 강편재의 경우 직류 자화 전류에 의한 표면 자장은 그 형상에 의한 영향으로 코너 부위에서는 면 중앙의 표면 자장치에 비해 30% 정도였으며, 교류 자화 전류에 의해서는 그 비율이 70%정도였다. 교류 자화 전류는 코너로부터 면중앙으로 10mm 되는 영역을 제외하고는 전 면에서 균일한 표면 자장을 발생하엿다. 대상체의 온도에 따른 자분의 흡착은 대상체의 온도 150℃까지는 큰 변화가 없으나 자분의 고착에 있어서 60℃ 이상의 고온재에 대해서는 융착 용매로 메틸렌 크로라이드를 사용하는 것이 부적합하였다. 자분의 총분사량은 자분 탐상능에 상당히 큰 영향을 미침을 확인하였고 이에 대한 정량적 평가를 행하였다. Dry magnetic particle inspection(MPI) was performed to detect the surface defects of steel ingot cast billets. Magnetic properties of several materials were characterized by the measurement of the B-H hysteresis curve. The inspection results were evaluated in terms of the magnetizing current, temperature, and the amount of magnetic particles applied to billets. Magnetic flux leakage near the defect site of interest was measured and with the results of calculation by the finite element method in the case of direct magnetizing current. Direct and alternating magnetizing currents for materials were deduced by the comparison of the inspections. Results of the magnetic particle inspection by direct magnetizing current were compared with those of finite element method calculations, which were verified by measuring magnetic leakage flux above the surface and the surface defects of the material. For square rods, due to the geometrical effect, the magnetic flux density at the edges along the length of the rods was about 30% of that at the center of rod face for a sufficiently large direct magnetizing current, while it was about 70% for an alternating magnetizing current. Thus, an alternating magnetizing current generates rather uniform magnetic flux density over the rods, except for the region on the face across about 10 mm from the edge. The attraction of the magnetic particle by the magnetic leakage field was nearly independent of the surface temperature of the billets up to 150℃. However, the temperature should have been limited below 60℃ for an effective fixing of gathered magnetic particles to the billet surface using methylene chloride. We also found that the amount of applied magnetic particles tremendously affected the detection capability.

Identification and expression profiles of chitin deacetylase genes in the rice leaf folder, Cnaphalocrocis medinalis

Hai-Zhong Yu,Ming-Hui Liu,Xue-Yang Wang,Xin Yang,Wan-Ling Wang,Lei Geng,Dong Yu,Xue-Lan Liu,Gui-Ying Liu,Jia-Ping Xu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

Chitin deacetylase (CDA) is an insect chitin degradation enzyme that catalyzes the deacetylation of chitin to form chitosan. In this study, combination of rapid-amplification of cDNA ends (RACE) technology with Cnaphalocrocis medinalis transcriptome database analysis revealed the presence of at least five C. medinalis CDAs (CmCDAs), which were CmCDA1, CmCDA2, CmCDA4, CmCDA5, and CmCDA6. The cDNA sequences of CmCDA1, CmCDA2, and CmCDA4 hadwhole open reading frame (ORF) for further analysis. Phylogenetic analysis indicated that insect CDAs could be categorized into five groups. CmCDAs' structural domain analysis revealed that all three CDAs contained the chitin deacetylase-like catalytic domain. CmCDA1 and CmCDA2 belong to Group I because they both contain the chitin-binding peritrophin-A domain (ChBD), low-density lipoprotein receptor class A domain (LDLa), and chitin deacetylase-like catalytic domain. CmCDA4 only contains ChBD and chitin deacetylase-like catalytic domain thus belongs to Group III. Tissue and developmental stage expression analysis showed that the expression levels of CmCDA1, CmCDA2, and CmCDA4 are significantly higher in the head than other tissues and also significantly higher in adults than in larvae. CmCDA5 had significantly higher expression in the integument than other tissues, suggesting potential roles in the process of degradation of chitin. In contrast, CmCDA5 showed relatively high expression in larvae. In conclusion, this study analyzed the cDNA sequences of three CDA genes and determined their expression and molecular characteristics, which provided a new sequence resource and improved the development of bio-pesticides and the biological pest control and contributed to management of this important agricultural pest.

Inhibitory Effect of Laser Surface Texturing in Dry Cutting of Titanium Alloy on Cemented Carbide Tool Wear

Quan Wan,Weizhe Zhong,Xiaoyu Hu,Shucai Yang 한국정밀공학회 2024 International Journal of Precision Engineering and Vol.25 No.3

Compared to conventional tools, the cutting performance of microtextured tools is significantly better. Laser surface texturing (LST) is the most important technique for producing a microstructure. The modification of microstructure properties of tools by LST has not received much attention. In this study, the effects of LST on the microstructural properties of tools were investigated, and the specific causes of the improvement in wear resistance of tools by laser micro texturing and the effects of micro-texture on adhesive wear of tools were revealed. In this study, microtextured tools with different laser parameters were fabricated and the test was conducted for cutting titanium alloys. The changes in tool microstructural properties and tool wear morphology were compared and analyzed. LST effectively reduces the release of co-elements during cutting, solidifies WC particles, and reduces the adhesive and oxidative wear of the tool. In addition, the micro-texture and surrounding annular protrusions significantly reduce cutting heat, which in turn reduces diffusive and abrasive wear. In this context, the morphology of the micro-texture facilitates adhesive fracture and peeling of the adhesive wear, while the annular protrusions help reduce tool wear. Their interaction significantly improves the wear resistance of laser-machined microtextured tools.

Optimal Placement of CRNs in Manned/Unmanned Aerial Vehicle Cooperative Engagement System

( Yun Zhong ),( Peiyang Yao ),( Lujun Wan ),( Yeming Xiong ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.1

Aiming at the optimal placement of communication relay nodes (OPCRN) problem in manned/unmanned aerial vehicle cooperative engagement system, this paper designed a kind of fully connected broadband backbone communication topology. Firstly, problem description of OPCRN was given. Secondly, based on problem analysis, the element attributes and decision variables were defined, and a bi-level programming model including physical layer and logical layer was established. Thirdly, a hierarchical artificial bee colony (HABC) algorithm was adopted to solve the model. Finally, multiple sets of simulation experiments were carried out to prove the effectiveness and superiority of the algorithm.

Experimental Study of the Salt Transfer in a Cold Sodium Sulfate Soil

Xusheng Wan,Fumao Gong,Mengfei Qu,Enxi Qiu,Changmao Zhong 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.4

Salt migration and accumulation are the main sources of salt expansion. To study the role of salt transport in soil, laboratory tests were conducted to simulate salt transfer under real conditions. Temperature, water content, salt content and soil displacement of a sodium sulfate soil were measured during the freezing process/freeze-thaw cycles. Meanwhile, Salt concentration was regressed bases on test data to investigate the movement of salt diffusion, in the process, the Pitzer ion model was employed to calculate the freezing point of saline soils to determine the frozen depth. Moreover, the amount of crystallization was estimated by the saturation curve of a sodium sulfate solution and the detected salt content. The results show that salt transfer in soil occurs as a result of numerous physicochemical processes and that the maximum salt transport occurred in the frozen fringe zone in the soil. Salt crystallization increases the effect of salt transfer in the soil unidirectional freezing process. In addition, salt expansion had an accumulative effect, and it increases as the number of freeze-thaw cycles increases. The quantity of salt that is transported increases as the salt content increases.