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최완수(Wahn Soo Choi),정계종(Kae Jong Chung),이주경(Joo Kyung Lee),박주웅(Joo Woong Park),이상훈(Sang Hoon Lee),이진복(Jin Bok Lee),이송락(Song Rag Lee),최신원(Shin Won Choi) 대한약학회 1993 약학회지 Vol.37 No.2
Serratia sp. Y-4 was isolated from soil. Many characteristics of the strain and optimum cultivation condition for protease production were investigated. The protease from Serratia sp. Y-4 was purified and studied for the properties of the enzyme. The isolated strain was identified to the genus Serratia. The strain was cultivated in 1%-casein, 0.5%-Na3PO4.7H2O, 0.1%-NaCl, 0.05%-KCl, 0.02%-MgSO4.7H2O, 0.02%-CaCl2.2H2O, 0.02%-ZnSO4.7H2O, 0.02%-MnCl2.4H2O and 0.5%-soy bean oil at pH 7.0 for 35 hrs. The enzyme was purified about 5.89 fold from the culture broth with 31.1% recovery and 19,613mc/mg through ultrafiltration, ammonium sulfate, DEAE-sephacel and Superose-12 chromatography. The purified enzyme was identified as one band by isoelectric focusing, SDS- and native-PAGE. It has maxium activity at 37oC and pH 9.0. Molecular weight of it is approx. 50 kD and pI is about 6.70. Its Km value for casein was 20mg/ml. 5 mM-EDTA, 5 mM-SDS, Ag+1, Cu+2, Hg+2 and Pb+2 inhibited the enzyme.
Choi, Wahn Soo,Seo, Dong Wan,Chang, Man Sik,Han, Jeung Whan,Hong, Sung Youl,Paik, Woon Ki,Lee, Hyang Woo 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1
The presence of L-arginine methylester (AME), L-arginine ethylester (AEE), or N-nitro-L-arginine methylester (NAME) in the growth media of Staphylococcus aureus increased the nitric oxide synthase (NOS) activity approximately 5-to 14-fold. The increase of NOS activity was confirmed by two assay methods, namely assaying the formation of L-[^3H] citrulline from L-[^3H] arginine and NO formation. The increase of NOS activity was most likely due to increased de novo synthesis, demonstrated by Western immunoblot analysis. The addition of methanol to the culture medium also increased the NOS activity as much as that found with the above three compounds. Evidence is presented to show that AME, AEE, or NAME gave rise to the formation of methanol in vivo by the action of intracellular esterase(s) and that methanol is subsequently involved in the induction of NOS in this bacterial system.
Identification of Nitric Oxide Synthase in Staphylococcus aureus
Choi, Wahn-Soo,Chang, Man-Sik,Han, Jeung-Whan,Hong, Sung-Youl,Lee, Hyang-Woo 성균관대학교 약학연구소 1997 成均藥硏論文集 Vol.9 No.1
The presence of nitric oxide synthase (NOS) in Staphylococcus aureus ATCC6538P was established by western blot analysis. The identity of citrulline formed from Larginine by the NOS was confirmed by both TLC and HPLC and the other product, No, by directly measuring the production of nitrogen oxides (NO_x) in the reaction mixture. The activity was inhibited by typical NOS inhibitors such as N-nitro-L-arginine methylester and N^G, N^G-dimethyl-L-arginine with the IC_50 of 4.9×10exp(-4) and 2.5×10exp(-4) M, respectively. The NOS activity was maximum at pH 6.5 and at 47.5℃. These results indicate that the NOS of S. aureus ATCC6538P is an isoform distinct from mammalian NOS.
A Turbidimetric Determination of Protein by Trichloroacetic Acid
Choi, Wahn-Soo,Chung, Kae-Jong,Chang, Man-Sik,Chun, Jae-Kwang,Lee, Hyang-Woo,Hong, Sung-Youl The Pharmaceutical Society of Korea 1993 Archives of Pharmacal Research Vol.16 No.1
Based on the turbidimetric response of protein with 50% trichloroacetic acid (TCA), this study aims to introduce an assay method for protein in solution. The standard procedure consists of mixing equal volume of sample solution (standard or unknown) with 50%-TCA solution and measuring the absorbance at 450 nm after 20 min. The absorbances of the solutions were almost stable over 120 min at room temperature. This assy method is simple, reproducible, and tolerant to many interfering substances. It can detect less amount than $10\mu$g/ml of bovin serum albumin. The assay method has low protein-to-protein variability over wide range of molecular weight.
Park, Hyun Jung,Choi, Wahn Soo,Lee, Won Young,Choi, Youngsok,Park, Chankyu,Kim, Jin Hoi,Hong, Kwon Ho,Song, Hyuk Elsevier 2018 Environmental toxicology and pharmacology Vol.58 No.-
<P><B>Abstract</B></P> <P>The pathogenesis of atopic dermatitis (AD) involves T helper 2 (Th2) cells, and effective therapies remain elusive due to the paucity of animal models. We aimed to develop a mouse model of an immune system aberration caused by allergen. Experiments were conducted in two phases. In experiment 1, BALB/c mice were sensitized with one of four chemical allergens – toluene diisocyanate (TDI), hexamethylene diisocyanate (HDI), trimellitic anhydride (TMA), or 2,4-dinitrochlorobenzene (DNCB) – for 3 weeks. Based on results of experiment 1, immunological features were compared between TMA-sensitized BALB/c mice and NC/Nga mice, after exposure to mite extracts, harmful chemicals and detergents in experiment 2. Sensitization by allergen caused a large number of pathological changes in the skin, and an increase in mast cell number. TMA-sensitized BALB/c mice models showed higher sensitivity to an environmental allergen than NC/Nga mice did. Overall, the initial sensitization with TMA leads to disturbances in Th2-mediated immunity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> To develop a mouse model of an immune system aberration caused by a chemical allergen. </LI> <LI> Th2-skewed immune response in TMA-sensitized mice closely mimics human AD. </LI> <LI> TMA-sensitized mice showed higher sensitivity to an environmental allergen than NC/Nga mice. </LI> </UL> </P>
Dong Ki Park,Wahn Soo Choi,Pyo-Jam Park,Eun Kyung Kim,Yong Jun Jeong,Se Young Choi,Koji Yamada,Jong Dai Kim,임병우 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.4
Cordyceps sinensis, one of the well-known fungi used in traditional Chinese medicine, is recognized to play a role in the metabolic process of inflammation and immunity. The purpose of this study was to evaluate the effects of water extracts of C. sinensis on the immune function of mesenteric lymph node (MLN) lymphocytes in C57Bl/6N mice. C. sinensis-treated mice were administered the respective extract by oral gavage for 4 weeks. Immunoglobulin E concentrations in serum and MLN lymphocytes were significantly lower in C. sinensis-treated mice than in control mice. In contrast, the immunoglobulin A concentration from the C. sinensis group was higher than that in control mice. C. sinensis increased the proportion of CD4+ and CD8+ T cells in MLN lymphocytes. C. sinensis significantly decreased interleukin-4 and interleukin-10 cytokine concentrations. Therefore, water extracts of C. sinensis modulate immune parameters through regulation of immunoglobulin production resulting from decreased T-lymphocyte helper 2 cytokine secretion and reduce cytokine secretion in MLN lymphocytes.