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Friendly production of bacterial inclusion bodies
Antonio Villaverde,Elena García-Fruitós 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.2
Protein aggregation is commonly observed in genetically engineered bacteria over-expressing foreign genes,in the context of protein production processes. Very often, recombinant polypeptides deposit as insoluble protein clusters named inclusion bodies, whose formation is driven by stereo-specific cross-molecular interactions between partially folded polypeptide chains. The formation of inclusion bodies has been historically considered as the main bottleneck in industrial production processes of proteins, since a wide diversity of protein species tend to aggregate in bacteria. As the formation of inclusion bodies can be eventually minimized but rarely prevented, aggregated polypeptides of industrial interest need to be refolded in vitro before use. However, the progressive understanding of the molecular and physiological mechanisms regulating aggregation has revealed that inclusion bodies contain significant amounts of biologically active protein species making them suitable for the straightforward use in different in vitro processes,as functional, particulate entities. Therefore, when formed by enzymes, inclusion bodies are catalytic particles ready for industrial use. As discussed here, the genetic background of the host bacteria and the protein production conditions can be adjusted to tune the biological and biophysical properties of bacterial inclusion bodies, to gain manipulability and to make them more biotechnologically friendly.
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Passive Internet-based Crane Teleoperation with Haptic Aids
Alejandro F. Villaverde,Cesáreo Raimúndez,Antonio Barreiro 제어·로봇·시스템학회 2012 International Journal of Control, Automation, and Vol.10 No.1
Crane operation is a challenging task, due to the combined problem of obstacle avoidance and load swing suppression in underactuated conditions. This paper presents a human-machine interface that increases the operator’s perception of a gantry crane’s workspace. With this aim, a virtual environment resembling the workspace is connected with a haptic device. This allows the user to receive not only visual but also tactile feedback, thus increasing maneuvering safety. Additionally, this capability is integrated in a teleoperation setup, adopting a passivity-based control approach that guarantees overall stability. This includes also the design of controllers by means of the IDA-PBC method. Ex-perimental results carried out with a laboratory crane show its feasibility for internet-based teleopera-tion and demonstrate the improvements on the system performance.
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Inés C. Oría,Juan E. Pizzala,Augusto M. Villaverde,Juan C. Spina,Analía V. Pasqua,Julio C. Lazarte,Oscar M. Mazza,Mariano M. Marcolongo 대한소화기내시경학회 2019 Clinical Endoscopy Vol.52 No.2
The pancreatoduodenal groove is a small area where pathologic processes involving the distal bile duct, duodenum, pancreatic head,ampulla of Vater, and retroperitoneum converge. Despite great advances in imaging techniques, a definitive preoperative diagnosis ischallenging because of the complex anatomy of this area. Therefore, surgical intervention is frequently required because of the inabilityto completely exclude malignancy. We report 3 cases of patients with different groove pathologies but similar clinical and imaging presentation, and show the essential roleof endoscopic ultrasound (EUS) in making a specific preoperative diagnosis, excluding malignancy in the first case, changing diagnosisin the second case, and confirming malignancy in the third case. EUS was a fundamental tool in this cohort of patients, not onlybecause of its ability to provide superior visualization of a diffcult anatomical region, but because of the ability to guide precise, realtimeprocedures, such as fine-needle aspiration.
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