http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Villa (검색결과 52 건)
- 무료
- 기관 내 무료
- 유료
-
Forced swimming stress increases natatory activity of lead-exposed mice
Araujo Ulisses C.,Krahe Thomas E.,Ribeiro-Carvalho Anderson,Gomes Regina A. A.,Lotufo Bruna M.,Moreira Maria de Fátima R.,de Abreu-Villaça Yael,Manhães Alex C.,Filgueiras Cláudio C. 한국독성학회 2021 Toxicological Research Vol.37 No.1
Recent evidence points to the relationship between lead toxicity and the function of the hypothalamic-pituitary-adrenal axis, which suggests that lead exposure could influence how an individual cope with stress. Here we test this hypothesis by investigating the behavioral effects of lead exposure in mice during the forced swimming test (FST), a parading in which animals are exposed to a stressful situation and environment. Swiss mice received either 180 ppm or 540 ppm of lead acetate (Pb) in their ad-lib water supply for 60–90 days, starting at postnatal day 30. Control (Ctrl) mice drank tap water. At the end of the exposure period, mice were submitted to a 5-min session of FST or to an open-field session of the same duration. Data from naïve animals showed that corticosterone levels were higher for animals tested in the FST compared to animals tested in the open-field. Blood-lead levels (BLL) in Pb-exposed mice ranged from 14.3 to 106.9 μg/dL. No differences were observed in spontaneous locomotion between Ctrl and Pb-exposed groups in the open-field. However, in the FST, Pb-treated mice displayed higher swimming activity than Ctrl ones and this effect was observed even for animals with BLL higher than 20 μg/ dL. Furthermore, significant differences in brain glutathione levels, used as an indicator of led toxicity, were only observed for BLL higher than 40 μg/dL. Overall, these findings suggest that swimming activity in the FST is a good indicator of lead toxicity and confirm our prediction that lead toxicity influences behavioral responses associated to stress.
-
-
( Thea Villa ),( Mijin Kim ),( Seikwan Oh ) 한국응용약물학회 2020 Biomolecules & Therapeutics(구 응용약물학회지) Vol.28 No.5
Fangchinoline (FAN) is a bisbenzylisoquinoline alkaloid that is widely known for its anti-tumor properties. The goal of this study is to examine the effects of FAN on arthritis and the possible pathways it acts on. Human fibroblast-like synovial cells (FLS), carrageenan/ kaolin arthritis rat model (C/K), and collagen-induced arthritis (CIA) mice model were used to establish the efficiency of FAN in arthritis. Human FLS cells were treated with FAN (1, 2.5, 5, 10 μM) 1 h before IL-1β (10 ng/mL) stimulation. Cell viability, reactive oxygen species measurement, and western blot analysis of inflammatory mediators and the MAPK and NF-κB pathways were performed. In the animal models, after induction of arthritis, the rodents were given 10 and 30 mg/kg of FAN orally 1 h before conducting behavioral experiments such as weight distribution ratio, knee thickness measurement, squeaking score, body weight measurement, paw volume measurement, and arthritis index measurement. Rodent knee joints were also analyzed histologically through H&E staining and safranin staining. FAN decreased the production of inflammatory cytokines and ROS in human FLS cells as well as the phosphorylation of the MAPK pathway and NF-κB pathway in human FLS cells. The behavioral parameters in the C/K rat model and CIA mouse model and inflammatory signs in the histological analysis were found to be ameliorated in FAN-treated groups. Cartilage degradation in CIA mice knee joints were shown to have been suppressed by FAN. These findings suggest that fangchinoline has the potential to be a therapeutic source for the treatment of rheumatoid arthritis.
-
-
G. Villa,C. C. Campisi,M. Ryan,F. Boccardo,P. Di Summa,M. Frascio,G. Sambuceti,C. Campisi 대한핵의학회 2019 핵의학 분자영상 Vol.53 No.1
Introduction) Lymphoscintigraphy is the gold standard for imaging in the diagnosis of peripheral lymphedema. However, there are no clear guidelines to standardize usage across centers, and as such, large variability exists. The aim of this perspectives paper is to draw upon the knowledge and extensive experience of lymphoscintigraphy here in Genoa, Italy, from our center of excellence in the assessment and treatment of lymphatic disorders for over 30 years to provide general guidelines for nuclear medicine specialists. Method) The authors describe the technical characteristics of lymphoscintigraphy in patients with limb swelling. Radioactive tracers, dosage, administration sites, and the rationale for a two-compartment protocol with the inclusion of subfascial lymphatic vessels are all given in detail. Results) Examples of lymphoscintigraphic investigations with various subgroups of patients are discussed. The concept of a transport index (TI) for semi-quantitative analysis of normal/pathological lymphatic flow is introduced. Different concepts of injection techniques are outlined. Discussion) It is past time that lymphoscintigraphy in the diagnosis of lymphatic disorders becomes standardized. This represents our first attempt to outline a clear protocol and delineate the relevant points for lymphoscintigraphy in this patient population.
-
( Erica Villa ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Cirrhosis traditionally has been considered a hypocoagulable state; recently it has become clear that is a rather composite conditionin which liver synthetic deficit rebalances coagulation by reducing both procoagulant and anticoagulant factors. This leadsto an unstable haemostatic balance with a lower threshold for either thrombosis or bleeding. In compensated cirrhosis, this balance,although unstable, is sufficient and no special measures (blood transfusions, FFP, platelets) are required, even for patientsundergoing invasive procedures. Accurate evaluation of coagulative profile (e.g. with TEG instead than with routine coagulativetests), leads to significantly lower use of blood products without additional risks for the patients (Hepatology. 2016Feb;63(2):566-73. doi: 10.1002/hep.28148. Epub 2015 Dec 9). Indeed, apart from the gastrointestinal tract, the occurrenceof spontaneous and procedure-related bleeding elsewhere in the body, whilst not uncommon, is less than ir could be expected.During the course of cirrhosis, however, in parallel with progression of severity of disease, thrombophilic events, like portal veinthrombosis (PVT), become a frequent event occurring up to 40% of patients with liver cirrhosis.LMWH has been shown to be safe and effective in the treatment of PVT in cirrhotic patients (Eur J Gastroenterol Hepatol. 2015Aug;27(8):914-9. doi: 10.1097/MEG. 0000000000000351.PVT causes deterioration of the clinical course, portal hypertensive complication and post-transplant mortality. Pathogenesisof PVT includes both local alterations, like blood flow reduction and endothelial activation, and systemic derangements. Systemicprohemostatic alteration include high von Willebrand factor, low ADAMTS 13, low levels of anticoagulants (antithrombin, proteinC-S) and increase in procoagulants like factor VIII.We have previously shown that Low Molecular Weight Heparin (LMWH) such as Enoxaparin is safe and effective both in treatmentand prevention of PVT. Furthermore, patients in prophylaxis with enoxaparin showed a lower rate of decompensation and abetter survival without bleeding complications. (Gastroenterology. 2012 Nov;143(5):1253-60.e1-4. doi: 10.1053/j.gastro.2012.07.018. Epub 2012 Jul 20). In such patients circulating bacterial DNA, endotoxemia and markers of inflammation wereattenuated compared to controls. These results therefore suggest a possible connection between enoxaparin and decrease ofendotoxemia and reduction of portal hypertension. Enoxaparin was safe and no significant side effects were encountered. Wehave also subsequently shown that Enoxaparin treatment does not increase bleeding risk in patients undergoing invasive ornon-invasive endoscopic procedures performed while on Enoxaparin (ILC2016-RS-3560).On the whole, there is sufficient evidence to support the use of LMWH in patients with advanced cirrhotic disease to preventnot only PVT but also progression of disease and decompensation. It is still a matter of debate if longer duration of Enoxaparintreatment could be even more beneficial. In the Gastro 2012 study, the favourable effect of Enoxaparin was lost few monthsafter stopping therapy. This is understandable if one thinks that Enoxaparin likely acts by modifying microcirculation both atthe intestinal and hepatic level and that this effect is bound strictly associated with drug administration.Confirmatory studies are on their way.
-
Barrios-Villa Edwin,Pacheco-Flores Brenda,Lozano-Zaraín Patricia,Del Campo-Ortega Rodolfo,de Jesús Ascencio-Montiel Ivan,González-León Margot,Camorlinga-Ponce Margarita,Gaytán Cervantes Francisco Javi 한국유전학회 2023 Genes & Genomics Vol.45 No.5
Background The dry root or stem bark of Fraxinus chinensis is a famous herb Qin Pi which is known for its anti-inflammatory, analgesic, anti-tumor, liver protective and diuretic pharmacological effects, the fundamental chemical components are coumarin, phenylethanol glycosides and flavonoids. However, it is difficult to clarify the secondary metabolite synthesis pathway and key genes involved in the pathway because of lack genome information of Fraxinus chinensis. Objective To generate a complete transcriptome of Fraxinus chinensis and to clarify the differentially expressed genes (DEGs) in leaves and stem barks. Methods In this study, full-length transcriptome analysis and RNA-Seq were combined to characterize Fraxinus chinensis transcriptome. Results A total of 69,145 transcripts were acquired and regarded as reference transcriptome, 67,441 transcripts (97.47%) were annotated to NCBI non-redundant protein (Nr), SwissProt, the Kyoto Encyclopedia of Genes and Genomes (KEGG) and eukaryotic orthologous groups (KOG) databases. A total of 18,917 isoforms were annotated to KEGG database and classified to 138 biological pathways. In total, 10,822 simple sequence repeat (SSRs) and 11,319 resistance (R) gene were classified to 18 types, and 3947 transcription factors (TFs) were identified in full-length transcriptome analysis. Additionally, 15,095 DEGs were detected by RNA-seq in leaves and barks, including 4696 significantly up-regulated and 10,399 significantly down-regulated genes. And 254 transcripts were annotated into phenylpropane metabolism pathway containing 86 DEGs and ten of these enzyme genes were verified by qRT-PCR. Conclusion It laid the foundation for further exploration of the biosynthetic pathway of phenylpropanoids and related key enzyme genes.
-
M. Gallastegi-Villa,A. Aranzabal,M.P. González-Marcos,B.A. Markaide-Aiastui,J.A. González-Marcos,J.R. González-Velasco 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.81 No.-
The effect of vanadia loading on the acidic and redox properties of VOx/TiO2 catalyst and their role in thesimultaneous reduction of NO with NH3 and oxidation of o-DCB is studied. Catalyst samples withdifferent proportions of VOx species, ranging from monomerics to V2O5 octahedral crystals have beenprepared. A relationship between VOx species and their acidic and redox properties was found. At lowtemperature, o-DCB is adsorbed mainly on Lewis sites, associated to monomeric species, but at hightemperature, o-DCB and NH3 compete for Brønsted sites associated to polymeric species.
-
-
DBpia연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
