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Alpha-Amylase Immobilization on Epoxy Containing Thiol-Ene Photocurable Materials
( Cakmakci ),( Emrah ),( Ozkan Danis ),( Serap Demir ),( Yusuf Mulazim ),( Memet Vezir Kahraman ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.2
Thiol-ene polymerization is a versatile tool for several applications. Here we report the preparation of epoxide groups containing thiol-ene photocurable polymeric support and the covalent immobilization of α-amylase onto these polymeric materials. The morphology of the polymeric support was characterized by scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) coupled with SEM was used to explore the chemical composition. The polymeric support and the immobilization of the enzyme were characterized by FTIR analysis. SEM-EDS and FTIR results showed that the enzyme was successfully covalently attached to the polymeric support. The immobilization efficiency and enzyme activity of α-amylase were examined at various pH (5.0-8.0) and temperature (30-80oC) values. The storage stability and reusability of immobilized α-amylase were investigated. The immobilization yield was 276 ± 1.6 mg per gram of polymeric support. Enzyme assays demonstrated that the immobilized enzyme exhibited better thermostability than the free one. The storage stability and reusability were improved by the immobilization on this enzyme support. Free enzyme lost its activity completely within 15 days. On the other hand, the immobilized enzyme retained 86.7% of its activity after 30 days. These results confirm that α-amylase was successfully immobilized and gained a more stable character compared with the free one.
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Morphology and Mechanical Properties of Thermoplastic Polyurethane/Colemanite Composites
Ferhat ?en,Seyfullah Madakba?,Emre Ba?turk,Memet Vezir Kahraman 한국고분자학회 2017 폴리머 Vol.41 No.6
The aim of this study was to improve thermal stability, mechanical and surface properties of thermoplastic polyurethane (TPU) with the addition of colemanite. The TPU/colemanite composites having various ratios of TPU and colemanite were prepared. The chemical structure of the prepared composites was investigated by Fourier transform infrared spectroscopy (FTIR). Thermal properties of the samples were evaluated by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Mechanical properties of the samples were characterized with stress-strain test. Hydrophobicity of the samples was determined by the contact angle measurements. Moreover, the surface morphology of the samples was investigated by a scanning electron microscopy - energy dispersive spectrometer mapping (SEMEDS). Finally, the obtained results prove that the prepared composites have good thermal, mechanical and surface properties and that they can be used in many applications such as the electronic devices, materials engineering and other emergent.
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Selective molecularly imprinted polymer for the analysis of chlorpyrifos in water samples
Oya Aydın Urucu,Aslı Beyler Çi gil,Hatice Birtane,Ece Kök Yetimo glu,Memet Vezir Kahraman 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.87 No.-
In this paper, a new molecularly imprinted solid-phase extraction (MISPE) method was described for thedetermination of the chlorpyrifos in water samples. A thiol-ene based UV-cured polymer was preparedby mixing glyoxal bis (-diallyl acetal), pentaerythritol tetrakis (3-mercaptopropionate), polyethyleneglycol diacrylate (PEGDA), and photoinitiator. Chlorpyrifos was added to the prepared polymer as atemplate analyte, and its analysis was performed by gas chromatography with mass detection (GC–MS). The influence of some analytical parameters was studied. We found that the use of the molecularlyimprinted polymer (MIP) UV-cured disc provides an easy, selective, and available solution for removingchlorpyrifos from water samples.
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