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Diversity of Endophytic Bacteria in Ginseng and Their Potential for Plant Growth Promotion
Regupathy Thamizh Vendan,Young Joon Yu,Sun Hee Lee,Young Ha Rhee 한국미생물학회 2010 The journal of microbiology Vol.48 No.5
Endophytic bacteria have been found in virtually every plant studied, where they colonize the internal tissues of their host plant and can form a range of different beneficial relationships. The diversity of bacterial endophytes associated with ginseng plants of varying age levels in Korea was investigated. Fifty-one colonies were isolated from the interior of ginseng stems. Although a mixed composition of endophyte communities was recovered from ginseng based on the results of 16S rDNA analysis, bacteria of the genus Bacillus and Staphylococcus dominated in 1-year-old and 4-year-old plants, respectively. Phylogenetic analysis revealed four clusters: Firmicutes, Actinobacteria, α-Proteobacteria, and γ-Proteobacteria, with Firmicutes being predominant. To evaluate the plant growth promoting activities, 18 representative isolates were selected. Amplification of nifH gene confirmed the presence of diazotrophy in only two isolates. Half of the isolates solubilized mineral phosphate. Except four, all the other endophytic isolates produced significant amounts of indole acetic acid in nutrient broth. Iron sequestering siderophore production was detected in seven isolates. Isolates E-I-3 (Bacillus megaterium), E-I-4 (Micrococcus luteus), E-I-8 (B. cereus), and E-I-20 (Lysinibacillus fusiformis) were positive for most of the plant growth promoting traits, indicating their role in growth promotion of ginseng.
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Nath, A.,Singh, J.K.,Vendan, S. Ezhil,Priyanka, Priyanka,Sinha, Shreya Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1
Prostate cancer (CaP) is a common reproductive cancer among men. This study was conducted to correlate the cancer incidence with Gangetic zone and to correlate the tumor marker prostate specific antigen (PSA) level in serum with different age groups and stage of malignancy. Patients suffering from CaP in the pathology unit of Mahavir Cancer Sansthan (Hospital and Research Centre), Patna, Bihar, India were studied from June 2009 to May 2010. PSA level in the serum of CaP patients was estimated by ELISA method. CaP incidence was highly recorded in Gangetic zone than the non-Gangetic zone. Maximum patients were in the 56 - 75 years age group with a marked predominance. Results of PSA examination showed that serum PSA level was not correlating with the age of patient and stage of malignancy. Significantly, elevated level of more than 10 ng/ml of PSA was recorded among the studied cancer patients. In this study, it is concluded that Gangetic zone habitat have high risk of CaP and elevated level of PSA was marked in Bihar, India.
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Molecular Diversity of Rhizobacteria in Ginseng Soil and Their Plant Benefiting Attributes
홍은혜,이선희,알 타미즈 벤단,이영하,Hong, Eun Hye,Lee, Sun Hee,Vendan, Regupathy Thamizh,Rhee, Young Ha The Microbiological Society of Korea 2012 미생물학회지 Vol.48 No.4
The purpose of this study was to investigate the molecular diversity of rhizobacteria associated with ginseng of varying age levels and their plant benefiting attributes. A total of 143 different isolates belonging to 15 different bacterial genera were recovered. Although variation was found in the rhizobacterial community due to age of the plant, majority of bacteria belong to Firmicutes (58%). In which, Bacillus was found to be the predominant genus irrespective of age of the ginseng. To assess the plant benefiting attributes, 30 representative isolates were selected. The results indicated that some of the isolates could exhibit multiple plant growth promoting traits like secretion of cell wall degrading enzymes, production of indole-3-acetic acid, synthesis of siderophores, solubilization of phosphates and soil pathogens inhibition. It can be suggested that strains of B. subtilis, B. amyloliquefaciens, B. velezensis, and B. licheniformis were positive for all the above traits, which have potential to be used as plant growth promoting inoculants to improve ginseng crop in the future. 연령을 달리하는 인삼토양 내 근권세균의 다양성과 이들 세균이 갖고 있는 식물생장에 유용한 특성에 대하여 조사하였다. 인삼 근권으로부터 총 15개 속에 포함되는 143 균주를 분리하였다. 인삼 연령에 따른 세균 군집의 변화가 있었으나, 분리 균주의 58%가 Firmicutes에 속하였다. 특히 Bacillus는 연령에 관계없이 가장 우점하는 속으로 나타났다. 이들 분리 균주 중 30개의 균주를 선발하여 세포벽 분해효소 분비, 인돌아세트산 생성, siderophore 생성, 인 가용화, 식물병원성 곰팡이의 저해능 등에 대하여 평가한 결과, 다수의 균주가 식물생장을 촉진할 수 있는 기능적 특성을 갖고 있었다. 이 중에서도 모든 조사 항목에 대하여 양성 반응을 보인 B. subtilis, B. amyloliquefaciens, B. velezensis, B. licheniformis 균주는 향후 인삼 경작을 위한 생장 촉진 미생물제제로 활용가치가 높을 것으로 기대된다.
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Characterizations of plasma sprayed composite coatings over 1020 mild steel
P. Vijayanand,Amitesh Kumar,K. R. Vijaya Kumar,A. Vinod,P. Kumaran,S. Arungalai Vendan 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.10
The present work deals with the coating of mild steel using plasma spray coating technique to improve surface properties for various industrial applications. Three different composite coating powders namely Al 2 O 3 :TiO 2 , Al 2 O 3 :TiO 2 : CNT and Al 2 O 3 : CNT were coated on the surface of mild steel for a thickness of 0.25 mm. Subsequently, the coated samples were tested for their mechanical characteristics namely tensile, compression and hardness followed by tribological investigations primarily comprising of wear analysis. Further, metallurgical analysis engrossing the Scanning electron microscopy, X-Ray diffraction and Energy-dispersive X-ray spectroscopy analysis were carried out to assess the homogeneity, spread uniformity and bond integrity of the developed samples. The test results revealed that the performance measure of Al 2 O 3 :TiO 2 : CNT is way ahead than other two powder coatings while carbon nanotube majorly contributes to the strength and quality of the surface coating.
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