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Prasain, Nutan,Lee, Man Ryul,Vemula, Sasidhar,Meador, Jonathan Luke,Yoshimoto, Momoko,Ferkowicz, Michael J,Fett, Alexa,Gupta, Manav,Rapp, Brian M,Saadatzadeh, Mohammad Reza,Ginsberg, Michael,Elemento, Nature Publishing Group, a division of Macmillan P 2014 Nature biotechnology Vol.32 No.11
The ability to differentiate human pluripotent stem cells into endothelial cells with properties of cord-blood endothelial colony–forming cells (CB-ECFCs) may enable the derivation of clinically relevant numbers of highly proliferative blood vessel–forming cells to restore endothelial function in patients with vascular disease. We describe a protocol to convert human induced pluripotent stem cells (hiPSCs) or embryonic stem cells (hESCs) into cells similar to CB-ECFCs at an efficiency of >10<SUP>8</SUP> ECFCs produced from each starting pluripotent stem cell. The CB-ECFC-like cells display a stable endothelial phenotype with high clonal proliferative potential and the capacity to form human vessels in mice and to repair the ischemic mouse retina and limb, and they lack teratoma formation potential. We identify Neuropilin-1 (NRP-1)-mediated activation of KDR signaling through VEGF<SUB>165</SUB> as a critical mechanism for the emergence and maintenance of CB-ECFC-like cells.
Seshagirirao, Kottapalli,Leelavathi, Chaganti,Sasidhar, Vemula Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.3
A cross-linked leucaena (Leucaena leucocephala) seed gum (CLLSG) matrix was prepared for the isolation of galactose-specific lectins by affinity chromatography. The matrix was evaluated for affinity with a known galactose-specific lectin from the seeds of snake gourd (Trichosanthes anguina). The matrix preparation was simple and inexpensive when compared to commercial galactose-specific matrices (i.e. about 1.5 US$/100 ml of matrix). The current method is also useful for the demonstration of the affinity chromatography technique in laboratories. Since leucaena seeds are abundant and inexpensive, and the matrix preparation is easy, CLLSG appears to be a promising tool for the separation of galactose-specific lectins.
Rajesh Kumar Kante,Silpa Somavarapu,Sandeep Vemula,Chandrika Kethineni,Maheshwara Reddy Mallu,Srinivasa Reddy Ronda 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.5
The recombinant human asparaginase (rhASP) plays an important role in the treatment of acute lymphoblastic leukemia. In the present work, volumetric mass transfer coefficient (kLa) values are derived from the E. coli cultivation under different agitation and aeration conditions, in order to improve the rhASP productivity. The aeration and agitation conditions were systematically optimized by the kLa. The maximum biomass (2.4 g/L) and rhASP (1.68 g/L) are achieved with the kLa of 0.024 s-1 at 1.5 lpm and 700 rpm process conditions. The kinetic properties of purified rhASP are also extensively studied and optimized for the maximal enzyme activity. The optimal pH, temperature and incubation duration conditions for accomplishing maximum enzyme activity are found to be 9.0, 40°C, and 30 min, respectively. The optimum substrate concentration and substrate specificity for the highest enzyme activity are of 0.07 M and L-asparagine, respectively. The enzyme activity (204 IU/mL) is significantly improved in the presence of sodium metal (Na+) ions and the inhibitors 2- mercaptoethanol, bromoacetic acid and urea have presented the highest inhibition rate on rhASP activity. The enzyme kinetic parameters Km and Vmax of the purified rhASP are recorded as 2.25 mM and 250 IU/mL, respectively. This work provides the extensive characteristic properties of rhASP enzyme, which enables us to place in a competition for the development of oncology drugs.
Spectroscopic Properties of Yb<sup>3+</sup>-Doped Silicate Glasses
Linganna, Kadathala,Ju, Seongmin,Kim, Bokhyeon,Han, Won-Taek,Venkatramu, Vemula,Jayasankar, Chalicheemalapalli Kulala De Gruyter 2018 Zeitschrift für physikalische Chemie Vol.232 No.1
<P><B>Abstract</B></P><P>Yb<SUP>3+</SUP>-doped silicate glasses (SiO<SUB>2</SUB>–Nb<SUB>2</SUB>O<SUB>5</SUB>–K<SUB>2</SUB>O–ZnF<SUB>2</SUB>) were prepared by a conventional melt quenching technique and their spectroscopic properties were investigated. The emission cross-section was found to be 0.53×10<SUP>−20</SUP>cm<SUP>2</SUP>at 1006 nm from the reciprocity method described by McCumber from the measured absorption spectrum of 1.0 mol% Yb<SUB>2</SUB>O<SUB>3</SUB>-doped glass. The lifetime (<I>τ</I><SUB>exp</SUB>) and figure of merit (<I>σ<SUB>em</SUB></I>×<I>τ</I><SUB>exp</SUB>) for the<SUP>2</SUP>F<SUB>5/2</SUB>→<SUP>2</SUP>F<SUB>7/2</SUB>transition of 1.0 mol% Yb<SUB>2</SUB>O<SUB>3</SUB>-doped silicate glass, were evaluated to be 0.50 ms and 0.26 cm<SUP>2</SUP>ms, respectively. The other spectroscopic parameters,<I>β</I><SUB>min</SUB>,<I>I</I><SUB>sat</SUB>and<I>I</I><SUB>min</SUB>were found to be 0.17, 29.00 kW/cm<SUP>2</SUP>and 4.90 kW/cm<SUP>2</SUP>, respectively. The results indicate that the present Yb<SUP>3+</SUP>-doped silicate glasses could be useful as laser gain media at 1006 nm.</P>
Nickel and nickel oxide nanocrystals selectively grafting on multiwalled carbon nanotubes
Prabhu Yendrapati Taraka,Rao Kalagadda Venkateswara,Kumari Bandla Siva,Sai Vemula Sesha,Pavani Tambur 나노기술연구협의회 2015 Nano Convergence Vol.2 No.2
Nickel and nickel oxide nanocrystals in their pure phase are carefully embellished by a facial method on oxygen-functionalized multi-walled carbon nanotubes (O-MWCNTs) using nickel nitrate (NN) was effectively accomplished for the first time by calcining them in hydrogen, nitrogen and air, respectively, at suitable temperatures. Nickel and nickel oxide nanocrystals impregnated O-MWCNTs were examined for its structure and morphology by various techniques, such as powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy and field emission scanning electron microscopy. The nanocrystals on the O-MWCNTs were determined of 15–20 nm size. Decorated nanocrystals on CNT’s have potential applications in semiconductor industries.
Srinivasa Reddy Ronda,Pavani Lakhsmi Chandrika Parupudi,Sandeep Vemula,Santhosh Tumma,Mahendran Botlagunta,Vijaya Saradhi Settaluri,Smita Lele,Suraj Sharma,Chari Kandala 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.10
This work describes the medium optimization of γ-Linolenic acid (GLA) production by Spirulina platensisusing one-factor and orthogonal array design methods. In the one-factor experiments, NaHCO3 (9 mg L−1), NaNO3(13.5 mg L−1) and MgSO4·7H2O (11.85 mg L−1) proved to be the best components for GLA production. The optimalpH for GLA production by the alga was 9.2. Based on the delta values, NaHCO3 showed the greatest effect on the GLAproduction of the various factors tested, followed in decreasing order by MgSO4·7H2O, NaNO3 and K2SO4. The max-imum GLA yield obtained was 19.2 mgL−1in the presence of optimum concentrations of NaHCO3 (20 g L−1), NaNO3(3 g L−1), MgSO4·7H2O (0.5 g L−1) and K2SO4 (1.5 g L−1). Because of the slow growth rate of the algae, the practiceof robust orthogonal array methods during the optimization of medium components can result in the production of anoptimal biomass and a higher GLA yield for nutraceutical applications.
CO2 fixation and lipid production by microalgal species
Srinivasa Reddy Ronda,Pavani Parupudi,Chandrika Kethineni,Pradip Babanrao Dhamole,Sandeep Vemula,Prasada Rao Allu,Mahendran Botlagunta,Sujana Kokilagadda 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.2
Microalgal species Nannochloropsis limnetica, Botryococcus braunii, and Stichococcus bacillaris were compared for their ability to grow, remove CO2, and accumulate lipids in their biomass under CO2-enriched atmosphere. Overall, N. limnetica outperformed the other two cultures and distinctly exhibited higher specific growth rate (0.999 d−1) and CO2 fixation rate (0.129 gL−1 d−1) with a high specific lipid yield (40% w/w). The volumetric CO2 fixation rate for all three species was validated with biomass productivity and mass transfer methods (P<0.005 and R2=0. 98). At 10% CO2, N. limnetica showed one-and-a-half times more carbon fixation efficiency over B. braunii, and S. bacillaris. On the other hand, total fatty acids of N. limnetica dispalyed an apparent increase in oleic acid. Whereas, under similar conditions, N. limnetica exhibited reduced eicosapentaenoic acid. These findings suggest that at high CO2 conditions, N. limnetica proved to be an efficient CO2 capture algal system and can be considered for biofuel applications.