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Vega L., Johana C. M.,Lee, Min Kyung,Qin, Ellen C.,Rich, Max,Lee, Kwan Young,Kim, Dong Hyun,Chung, Hee Jung,Leckband, Deborah E.,Kong, Hyunjoon Royal Society of Chemistry 2016 Journal of Materials Chemistry B Vol.4 No.42
<P>Living cells are being extensively studied to build functional tissues that are useful for both fundamental and applied bioscience studies. Increasing evidence suggests that cell-cell adhesion controlled by the intercellular cadherin junction plays important roles in the quality of the resulting engineered tissue. These findings prompted efforts to interrogate biological effects of cadherin at a molecular scale; however, few efforts were made to harness the effects of cadherin on cells cultured in an <I>in vivo</I>-like three dimensional matrix. To this end, this study reports a hydrogel matrix three dimensionally functionalized with a controlled number of Fc-tagged recombinant N-cadherins (N-Cad-Fc). To retain the desired conformation of N-Cad, these cadherins were immobilized and oriented to the gel by anti-Fc-antibodies chemically coupled to gels. The gels were processed to present N-Cad-Fc in uniaxially aligned microchannels or randomly oriented micropores. Culturing cortical cells in the functionalized gels generated a large fraction of neurons that are functional as indicated by increased intracellular calcium ion concentrations with the microchanneled gel. In contrast, direct N-Cad-Fc immobilization to microchannel or micropore walls of the gel limited the growth of neurons and increased the glial to neuron ratio. The results of this study will be highly useful to organize a wide array of cadherin molecules in a series of biomaterials used for three-dimensional cell culture and to regulate phenotypic activities of tissue-forming cells in an elaborate manner.</P>
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( Vega Pradana Rachim ),( Jinyoung An ),( Quan Ngoc Pham ),( Wan-young Chung ) 한국센서학회 2018 센서학회지 Vol.27 No.1
In this paper, a 32-variable pulse position modulation (32-VPPM) scheme is proposed to support a red-green-blue light-emitting-diode (RGB-LED)-based optical camera communication (OCC) system. Our proposed modulation scheme is designed to enhance the OCC data transmission rate, which is targeted for the wearable biomedical data monitoring system. The OCC technology has been utilized as an alternative solution to the radio frequency (RF) wireless system for long-term self-healthcare monitoring. Different biomedical signals, such as electrocardiograms, photoplethysmograms, and respiration signals are being monitored and transmitted wirelessly from the wearable biomedical device to the smartphone receiver. A common 30 frames per second (fps) smartphone camera with a CMOS image sensor is used to record a transmitted optical signal. Moreover, the overall proposed system architecture, modulation scheme, and data demodulation are discussed in this paper. The experimental result shows that the proposed system is able to achieve > 9 kbps using only a common smartphone camera receiver.
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Vega, R.A.,Hidari, H.,Matsunaga, N.,Kuwayama, H.,Manalo, D.D.,Lee, H.G.,Hata, H. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.7
In a herd of 24 spring-born steers, plasma leptin and performance of selected purebred (n=5) and backcrossed Hereford (n=5) were compared in a year-round summer grazing and winter feedlot fattening. Bimonthly blood collection and body weight measurement were accomplished. The plasma samples were analyzed for leptin, insulin, total cholesterol, triglyceride, NEFA and glucose. The experimental design utilized one-way ANOVA with breed as the treatment. The purebred obtained higher plasma NEFA (p<0.001) compared to backcross, regardless of seasonal feeding systems (SFS). The backcross showed gradual increase and nonresponsiveness of plasma leptin to SFS. During summer grazing, attenuation of plasma leptin and sudden elevation when shifted to winter feedlot fattening were observed in purebred. Plasma leptin obtained linear relationship with body weight of purebred (r=0.53;p<0.001) and backcrossed Hereford (r=0.49; p<0.01). The purebred and backcrossed Hereford, when shifted to summer grazing, resulted to sustained and restricted daily gain, respectively. Therefore, cattle breeds of higher growth potential exhibit significant elevation of plasma leptin after 400 kg BW, when animal starts to deposit significant body fat.
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Vega, R.A.,Lee, H.G.,Kuwayama, H.,Matsunaga, N.,Hidari, H. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.5
This experiment was performed to understand the changes in plasma leptin in association with plasma IGF-1, body weight and ADG from early growing to late finishing stages of Holstein steers. Blood collection was performed by arterial vein puncture at selected monthly ages of 1 (54 kg), 2.6 (103 kg), 7.2 (205 kg), 13.5 (314 kg), 16.9 (414 kg), 22.2 (550 kg), 24.9 (626 kg) and 27.4 months (695 kg). The blood was analyzed for leptin using the multi-species leptin RIA with recombinant bovine leptin (rbleptin) as standard, plasma IGF-1 was also measured using RIA. Against the standard rbleptin, the multi-species Leptin RIA system's sensitivity, cross reactivity, slope and recovery of 41.0 ng/ml rbleptin in plasma were 4.9 ng/ml, 11.22%, -1.396 and 97.8%, respectively. Plasma leptin measured were more than 5.0 ng/ml, which enable multi-species RIA system to investigate plasma leptin in normal growing steers. Body weight resulted to a highly significant second-degree polynomial relationship with plasma leptin (q=0.54, p<0.0001) and plasma IGF-1 (q=0.44, p<0.0001) from 1 to 27.4 monthly ages. However, the second-degree polynomial curve of plasma leptin and IGF-1 differs showing a concave and convex curvilinear relationship, respectively. ADG was not significantly associated to plasma leptin (r=0.06, p>0.05) and plasma IGF=1 (r=0.06, p>0.05) from 1 to 27.4 monthly ages. Low coefficient, but significant associated increase of plasma leptin and IGF-1 (r=0.12, p<0.008) from 1 to 27.4 months was observed. The uncoordinated increases of plasma IGF-1 at growing and plasma leptin at fattening period, may indicate (1) indirect involvement of endogenous IGF-1 on leptin secretion, and (2) IGF-1 level may signify lean and bone accretion while plasma leptin may mirror body fatness across the monthly ages of Holstein steers.
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( Vega Pradana Rachim ),( Sung Chul Kang ),( Wan Young Chung ),( Tae Ha Kwon ) 한국센서학회 2014 센서학회지 Vol.23 No.1
Noncontact electrocardiogram (ECG) measurement using capacitive-coupled technique is a very reliable long-term noninvasive health-care remote monitoring system. It can be used continuously without interrupting the daily activities of the user and is one of the most promising developments in health-care technology. However, ECG signal is a very small electric signal. A robust system is needed to separate the clean ECG signal from noise in the measurement environment. Noise may come from many sources around the system, for example, bad contact between the sensor and body, common-mode electrical noise, movement artifacts, and triboelectric effect. Thus, in this paper, the extended Kalman filter (EKF) is applied to denoise a real-time ECG signal in capacitive-coupled sensors. The ECG signal becomes highly stable and noise-free by combining the common analog signal processing and the digital EKF in the processing step. Furthermore, to achieve ubiquitous monitoring, android-based application is developed to process the heart rate in a realtime ECG measurement.
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Vega, R.A.,Hidari, H.,Kuwayama, H.,Suzuki, M.,Manalo, D.D. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.3
Six 16 months old Holstein steers were offered ad libitum feed for 7 months, to determine the (1) relationships of backfat thickness (BFT) to plasma leptin, and insulin; and (2) associations of TDN intake/kg body weight (BW) to plasma leptin, BFT and insulin. Feed intake, body weight and BFT were measured on selected monthly ages from day 1 to 8, day 1 and 8, and day 8, respectively. Blood was sampled on day 8 and the plasma was analyzed for leptin, insulin, glucose, NEFA, total cholesterol and triglyceride. Body weight and BFT increased, while TDN intake per kg BW decreased from 16 to 23 months old. Plasma leptin increased and mimicked the level of insulin, resulting to significant correlation (r=0.54; p<0.002). TDN intake was negatively related to plasma leptin (r=0.49; p<0.004), insulin (r=0.41; p<0.02) and BFT at 12 to 13th rib (r=0.48; p<0.005). Backfat thickness at 12 to 13th rib was positively related to plasma leptin (r=0.45; p<0.01). Negative associations of TDN intake with plasma leptin and BFT during finishing period suggest long-term involvement of adipose tissues in the feed intake regulation of steers fed high concentrate diet.
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( Roberto Vega Labanda ) 한국스페인어문학회(구 한국서어서문학회) 2019 스페인어문학 Vol.0 No.93
Although pronunciation plays a crucial role in oral communication, still very little attention, if any, is usually paid to it in most textbooks and courses of Spanish as a foreign language. South Korea does not constitute any exception to this situation, as learners in this country are hardly given any chances to satisfactorily understand and practice Spanish pronunciation. In fact, most textbooks and courses try to settle pronunciation in a few pages or a couple of sessions, usually making no clear distinction between phonology and orthography, while ignoring most prosodic elements. The approach that most textbooks and courses of Spanish as a foreign language to teach the target language could explain many of the most common problems that South Korean learners face when dealing with oral Spanish. The purpose of this article is to point out the relation between those problems in pronunciation and the methodological approach taken regarding this very aspect, while making some proposals to help learners to overcome those problems tracing them to their roots.
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Wearable-band type visible-near infrared optical biosensor for non-invasive blood glucose monitoring
Rachim, Vega Pradana,Chung, Wan-Young Elsevier 2019 Sensors and actuators. B, Chemical Vol.286 No.-
<P><B>Abstract</B></P> <P>Diabetes is a worldwide-serious problem that can only be delayed or prevented by a regular monitoring of blood glucose (BG) concentration level. Continuous monitoring systems allow subjects to prepare the diabetes management strategy and prevent the long-term complications diseases. Until now, most studies utilize various biofluids such as sweat, tears and saliva that have serious unresolved setback such as expensive material, sensor stability, sensor calibration and long-settling time. Therefore, we developed a novel BG sensor which is cost efficient and highly wearable with a small data acquisition time window that allow a non-invasive, long-term continuous blood glucose monitoring (CGM) system. The novel biosensor exploits a unique information of the pulsatile to continuous components of the arterial blood volume pulsation during the change of blood glucose (BG) concentration at the wrist tissue. The reflected optical signal was measured in the combine visible-near infrared (Vis-NIR) spectroscopy. An in-vivo experiment which enclosed 12 volunteers in a two-hour modified carbohydrate-rich meals reached the average correlation coefficient ( <SUB> R p </SUB> ) between the estimated and reference BG concentration of 0.86, with the standard prediction error (SPE) of 6.16 mg/dl. Moreover, the full-day experiment was also conducted to test the reliability of the proposed sensor. Results showed that the created model in the previous day, may estimate a full-day BG concentration which was done in next day with an adequate performance.</P>
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