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P3 Promoter of Bovine Papillomavirus Type - 1
Choe, Joon Ho,Peter Vaillancourt,Michael Botchan 한국유전학회 1988 Genes & Genomics Vol.10 No.4
In attempt to map the structure of transcripts which contain coding sequence from the E1 and E8 ORFs of BPV-1, we have identified a new promoter which is internal to the E1 ORF. S1 nuclease protection and primer extension analysis of poly(A) cytoplasmic RNA from ID 13 cells reveal the presence of a pair of 5′ ends at nucleotides 886 and 896 which correlate well with the 5′ ends of a class of cDNA clones. In order to determine whether these 5′ ends corresponded to a functional promotor, the BPV-1 Pst I fragment from 576-1299 was tested in an in vitro transcription assay using HeLa cell extract. Primer extension analysis of the in vitro transcription products showed the presence of two cap sites which were the same as those observed for in vivo mRNA, and whose transcription was α-amanitin sensitive. Using a set of overiapping, uniformly labelled SP6 probes from nucleotides 576-1299 we showed by RNase protection analysis that the vast majority of transcripts which initiate from this promotor use the splice donor at nucleotide 1235 previously mapped. This confirms that the most abundant class of P3 cDNAs uses this leader exon. These transcripts are moderately abundant relative to the bulk of the BPV-1 mRNAs: the P3 leader exon-containing transcripts are estimated to be about 5-fold less abundant than the set of E6, E7 and E6/E7 transcripts which contain exons which use the splice donor at nucleotide 865. Analogous promotors and leader exons located in the 5′ region of the E1 ORF have been identified in HPV-6 and HPV-11.
Genes Involved in Interleukin-1 Receptor Type II Activities Are Associated With Asthmatic Phenotypes
Anne-Marie Madore,Vanessa T. Vaillancourt,Emmanuelle Bouzigon,Chloé Sarnowski,Florent Monier,Marie-Hélène Dizier,Florence Demenais,Catherine Laprise 대한천식알레르기학회 2016 Allergy, Asthma & Immunology Research Vol.8 No.5
Purpose: Interleukin-1 (IL-1) plays a key role in inflammation and immunity and its decoy receptor, IL-1R2, has been implicated in transcriptomic and genetic studies of asthma. Methods: Two large asthma family collections, the French-Canadian Saguenay—Lac-St-Jean (SLSJ) study and the French Epidemiological Study on the Genetics and Environment of Asthma (EGEA), were used to investigate the association of SNPs in 10 genes that modulate IL-1R2 activities with asthma, allergic asthma, and atopy. Gene-gene interactions were also tested. Results: One SNP in BACE2 was associated with allergic asthma in the SLSJ study and replicated in the EGEA study before statistical correction for multiple testing. Additionally, two SNPs in the MMP2 gene were replicated in both studies prior to statistical correction and reached significance in the combined analysis. Moreover, three gene-gene interactions also survived statistical correction in the combined analyses (BACE1-IL1RAP in asthma and allergic asthma and IL1R1-IL1RAP in atopy). Conclusions: Our results highlight the relevance of genes involved in the IL-1R2 activity in the context of asthma and asthma-related traits.
Multi-target-directed phenol–triazole ligands as therapeutic agents for Alzheimer's disease
Jones, Michael R.,Mathieu, Emilie,Dyrager, Christine,Faissner, Simon,Vaillancourt, Zavier,Korshavn, Kyle J.,Lim, Mi Hee,Ramamoorthy, Ayyalusamy,Wee Yong, V.,Tsutsui, Shigeki,Stys, Peter K.,Storr, Tim Royal Society of Chemistry 2017 Chemical Science Vol.8 No.8
<▼1><P>A series of multi-target-directed ligands are described that bind Cu, act as antioxidants, modulate Aβ peptide aggregation, and abolish Aβ toxicity in primary neurons.</P></▼1><▼2><P>Alzheimer's disease (AD) is a multifactorial disease that is characterized by the formation of intracellular neurofibrillary tangles and extracellular amyloid-β (Aβ) plaque deposits. Increased oxidative stress, metal ion dysregulation, and the formation of toxic Aβ peptide oligomers are all considered to contribute to the etiology of AD. In this work we have developed a series of ligands that are multi-target-directed in order to address several disease properties. 2-(1-(3-Hydroxypropyl)-1<I>H</I>-1,2,3-triazol-4-yl)phenol (<B>POH</B>), 2-(1-(2-morpholinoethyl)-1<I>H</I>-1,2,3-triazol-4-yl)phenol (<B>PMorph</B>), and 2-(1-(2-thiomorpholinoethyl)-1<I>H</I>-1,2,3-triazol-4-yl)phenol (<B>PTMorph</B>) have been synthesized and screened for their antioxidant capacity, Cu-binding affinity, interaction with the Aβ peptide and modulation of Aβ peptide aggregation, and the ability to limit Aβ<SUB>1–42</SUB>-induced neurotoxicity in human neuronal culture. The synthetic protocol and structural variance incorporated <I>via</I> click chemistry, highlights the influence of R-group modification on ligand-Aβ interactions and neuroprotective effects. Overall, this study demonstrates that the phenol–triazole ligand scaffold can target multiple factors associated with AD, thus warranting further therapeutic development.</P></▼2>
( Philippe Gendron ),( Pascal Dufresne ),( Louis Laurencelle ),( Francois Trudeau ),( Simon Bergeron-vaillancourt ),( Anthony Bonal ),( Claude Lajoie ) 한국스포츠정책과학원(구 한국스포츠개발원) 2016 International Journal of Applied Sports Sciences Vol.28 No.1
The purpose was to examine improvements of performance and different physiological parameters in ten trained male cross-country mountain bikers after a supra-maximal interval training (SMIT) program of 8 sessions. Each session comprised 60 to 90 repeated 1-minute bouts consisting of a 20-s effort at 115% of maximal aerobic power (MAP), followed by 40-s recovery at 50% of MAP. Performance time to complete the virtual time-trial performance test was reduced significantly by -4.2%. Pulmonary sub-maximal oxygen consumption and deoxyhemoglobin in vastus lateralis decreased significantly by .4.7% and -21.9% respectively during the MAP test. Our findings suggest that SMIT program might improve performance and cycling efficiency in XC mountain bikers.
POLARIMETRY OF DG TAU AT 350 μm
Krejny, M.,Matthews, T. G.,Novak, G.,Cho, J.,Li, H.,Shinnaga, H.,Vaillancourt, J. E. IOP Publishing 2009 The Astrophysical journal Vol.705 No.1
<P>We present the first 350 mu m polarization measurement for the disk of the T Tauri star (TTS) DG Tau. The data were obtained using the SHARP polarimeter at the Caltech Submillimeter Observatory. We measured normalized Stokes parameters q = -0.0086 +/- 0.0060 and u = -0.0012 +/- 0.0061, which gives a 2 sigma upper limit for the percent polarization of 1.7%. We obtain information about the polarization spectrum by comparing our 350 mu m measurement with an 850 mu m polarization detection previously published for this source. Comparing the two measurements in Stokes space ( not in percent polarization) shows that the two data points are not consistent, i.e., either the degree of polarization or the angle of polarization (or both) must change significantly as one moves from 850 mu m to 350 mu m. This conclusion concerning the polarization spectrum disagrees with the predictions of a recent model for TTS disk polarization. We show that this discrepancy can be explained by optical depth effects. Specifically, we demonstrate that if one were to add more mass to the model disk, one would expect to obtain a model polarization spectrum in which the polarization degree falls sharply with increasing frequency, consistent with the observations at the two wavelengths. We suggest that multiwavelength polarimetry of TTS disk emission may provide a promising method for probing the opacity of TTS disks.</P>