Assessment of Hepatic Steatosis Improvement Using Controlled Attenuation Parameter in Patients with Non-Alcoholic Fatty Liver Disease under Regular Follow Up

( Kyu Sik Jung ),( Jun Yong Park ),( Mi Na Kim ),( Hana Park ),( Yun Bin Lee ),( Joo Ho Lee ),( Yeonjung Ha ),( Beom Kyung Kim ),( Seung Up Kim ),( Sang Hoon Ahn ),( Do Young Kim ),( Hana Park ),( Kyu 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: The change of hepatic steatosis in non-alcoholic fatty liver disease (NAFLD) patients under regular follow up has not been widely investigated. We investigated the serial changes of hepatic steatosis assessed by controlled attenuation parameter (CAP) and the predictors for improvement of hepatic steatosis in NAFLD patients. Methods: Among 513 NAFLD patients diagnosed upon ultrasound and CAP > 250 dB/m at baseline, 304 patients underwent repeated CAP measurement at baseline and at follow up. The improvement of hepatic steatosis was defined as a >10% decline of CAP value from the baseline. Results: Mean age of 304 patients was 58.8 ± 11.4 years and 80.1% were male. The mean CAP value significantly decreased from baseline to follow up (292.3 dB/m to 277.1 dB/m, P<0.001). During the median follow up of 15.3 (9.5-20.3) months, 34.5% patients showed improvement of hepatic steatosis. In a univariate analysis, low body mass index (BMI), low weight, low liver stiffness value, low triglyceride level, and low ALT at follow up were predictors for improvement of hepatic steatosis. In multivariate analyses, low weight (P=0.004; hazard ratio [HR],0.965; confidence interval [CI], 0.941-0.988) and low ALT (P=0.004; HR,0.971;CI, 0.951-0.990) at follow up, or low BMI (P=0.006; HR,0.881; CI, 0.805-0.964) and low ALT (P=0.004; HR,0.971; CI, 0.951-0.990) at follow up were the independent predictors. In patients with weight reduction more than 10% (n=22), CAP value significantly decreased (from 288.0 dB/m to 228.9dB/m, P<0.001). Conclusions: Weight reduction, and ALT decrease were independent predictors for improvement of hepatic steatosis. Long term effect of weight reduction on improvement of hepatic steatosis and fibrosis should be investigated further.

환동해권 경제교류협력의 평가와 발전 방안

최승업(Choi Seung Up) 한국지역개발학회 1996 韓國地域開發學會誌 Vol.8 No.3

In recent years, movements of cooperation and conciliation among the nations in East-Sea rim region are springing up. Many local governments in Russian Far East region, Chinese North-East region, Japanese West region, and Korean East region have been interested in this movements and already held several meetings to set up basis for the good relationship. But lots of difficulties are founded in the level of local government. Although Korean central government has not set up yet general policies at the level of entire country for responding to such movements, Kangwon-do, as a part of the regional development and globalization strategy, announced it`s plans for so called East-Sea Rim KARTELL Strategy in 1994 and held the Governors conference of Local Governments in the East-Sea rim in 1994 and 1996. In 1996, Donghae-si also held the conference of major cities in East-Sea rim region. These efforts have a significant meaning in that Kangwon-do has constructively met the challenges of the East-Sea rim era. East-Sea rim countries have interchanged each other lots of personnels and cultural and athletic groups so far to build up trust. But now all are seeking realistic cooperation plans and practical economic benefits from them. Thus, from now on, the purpose of meetings and interchanges among them should be focused on the promotion of trade, investments, co-op development projects, etc. It could only lead the East-Sea rim countries to tie stronger and longer.

UPS를 고려한 계통연계 전류제어형 인버터 시스템

이성룡(Seong-Ryong Lee),이수원(Su-Won Lee),고성훈(Sung-Hun Ko),강대업(Dae-Up Kang) 대한전기학회 2006 대한전기학회 학술대회 논문집 Vol.2006 No.7

본 논문에서는 계통연계형 인버터에서 요구되는 전력품질 개선 기능, 부하수요관리 기능, 무정전전원공급 기능 등을 전류제어형 전압원 인버터 시스템에서 통합적으로 수행할 수 있는 UPS를 고려한 계통연계형 전류제어 인버터를 제안한다. 제안된 시스템은 평상시에는 무효전력 보상, 고조파 저감, 부하수요관리 등의 PCS(Power Conditioning System) 기능을 을 수행하고 계통 고장시에는 배터리에 저장된 에너지를 이용하여 부하에 즉시 정전압을 공급하는 UPS 기능을 수행한다. 본 연구에서는 제안된 시스템의 동작원리 및 제어 알고리즘을 이론적으로 해석하고 시뮬레이션을 통하여 유용성을 확인한다.

Dkk-1 Promotes Angiogenesis through Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma

( Sang Hyun Seo ),( Hye Jung Park ),( Kyungjoo Cho ),( Hye Won Lee ),( Beom Kyung Kim ),( Jun Yong Park ),( Do Young Kim ),( Sang Hoon Ahn ),( Seung Up Kim ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Dickkopf-1(DKK1), a negative regulator of the Wnt/ ß-catenin pathway, has been recently found to be up-regulated in hepatocellular carcinoma (HCC). However, the biological function of DKK1 in HCC has not yet been well documented. Our previous in vitro data suggest that DKK1 can enhance angiogenesis by endothelial cell, independent of the Wnt signaling pathway. This study aimed to investigate the tumorigenic potential and angiogenic role of DKK1 in mouse model. Methods: We assessed tumorigenic functions of DKK1 in Hep3B cells expressing endogenous DKK-1 and in DKK1-deficient Hep3B cells created using CRISPR/Cas9 technology. These edited cells were injected subcutaneously in immunosuppressed mice and tumor growth was followed for 6 weeks. With the evidence of tumorigenic potential in DKK1, transgenic mouse models expressing DKK-1 or luciferase were developed using hydrodynamic transfection. Transposons encoding an activated form of human H-RAS were mixed with transposons encoding either DKK1 or luciferase. All mice were monitored at least twice per week and sacrificed when moribund. Subcutaneous tumors and tumor-bearing livers were formalin fixed for hematoxylin- eosin and immunofluoroscence staining. Results: DKK1-deficient Hep3B xenografts exhibited significantly less growth compared to control Hep3B cells expressing DKK1. In addition, the forced expression of DKK1 with H-RAS through the hydrodynamic transfection formed many tumors in the liver, compared to luciferase liver. We investigated the expression of angiogenesis markers, including CD31, VEGFR2 and mesenchymal markers, including vimentin, fibronectin in the subcutaneous tumors and tumor-bearing livers. Quantity of angiogenic and mesenchymal cells were found to be reduced in the established DKK1 homozygous knockout mice (all P<0.05). Taken together, it was confirmed that the expression of CD31 (P<0.0001), VEGFR2 (P<0.0001), vimentin, and fibronectin (P<0.0001) were up-regulated with DKK1 in the mouse liver. Conclusions: Our findings indicate that DKK1 appears to facilitate angiogenesis, and the progression of HCC through inducing the EMT.

심부정맥 혈전증의 도관유도 혈전용해술 또는 약물기계적 혈전제거술 후에 하대정맥 여과기 제거율과 임상적인 특징

노효근(Hyo Keun No),김중석(Joong Suck Kim),김장용(Jang Yong Kim),김경래(Kyung Rae Kim),신석환(Seok-Hwan Shin),안승익(Seung-Ik Ahn),조영업(Young Up Cho),김세중(Sei Joong Kim),이건영(Keon-Young Lee),허윤석(Yoon-Seok Hur),최선근(Sun Keu 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.79 No.6

Purpose: Inferior vena cava (IVC) filter is commonly practiced to prevent pulmonary embolism during endovascular therapy of deep vein thrombosis (EndoDVT). When the thrombus is trapped inside the filter during intervention, its removal is quite challenging. The purpose of this study is to determine retrieval rates of IVC filter after EndoDVT and its characteristics. Methods: Patients who underwent EndoDVT in Inha Unversity Hospital from June 2004 to May 2009 were reviewed retrospectively. Retrievable IVC filter was inserted before EndoDVT. EndoDVT was done by catheter directed thrombolysis or pharmacomechanical thrombectomy using urokinase. IVC filter retrieval was decided according to computed tomography after 2 weeks. Results: 126 patients were treated with EndoDVT. Optease (n=101) and Tulip (n=25) IVC filters were inserted. IVC filters were retrieved in 42.9% (54/126). There was no IVC filter related complication during its insertion and removal. IVC filter was not retrieved in 72 patients. Reasons for its failure include residual thrombosis in IVC filter (n=28), high risk for recurrent DVT (n=34), massive pulmonary embolism (n=8), and death (n=2). Residual thrombus inside IVC filter disappeared in 5 patients during 6-month follow up. Conclusion: IVC filters retrieval rate after EndoDVT was 42.9%. This can be improved by thorough patient follow up and extended retrievability.

Comprehensive analysis of time- and dose-dependent patterns of gene expression in a human mesenchymal stem cell line exposed to low-dose ionizing radiation.

Jin, Young-Woo,Na, Young-Ji,Lee, Young-Ju,An, Sungkwan,Lee, Jung Eun,Jung, Meeseon,Kim, Heesun,Nam, Seon Young,Kim, Cha Soon,Yang, Kwang Hee,Kim, Seung Up,Kim, Woo Kyung,Park, Woong-Yang,Yoo, Keun-You National Hellenic Research Foundation 2008 ONCOLOGY REPORTS Vol.19 No.1

<P>We focused on the transcriptional responses induced by low and very low doses of ionizing radiation with time effect. Regardless of their importance only a few limited studies have been done. Here we applied a large-scale gene transcript profile to elucidate the genes and biological pathways. Immortalized human mesenchymal stem cells were irradiated with 0.01, 0.05, 0.2 and 1 Gy of gamma radiation and total RNA was extracted from each cell line at 1, 4, 12 and 48 h after exposure. The essential transcriptional responses were identified according to dose and time. A total of 6,016 genes showed altered expression patterns at more than one time point or dose level among the investigated 10,800 genes. Genes that showed dose-dependent expression responses were involved in signal transduction, regulation of transcription, proteolysis, peptidolysis and metabolism. Those that showed time-dependent responses were divided into two distinct groups: the up-and-down group was associated with 'cellular defense mechanisms' such as apoptosis, cell adhesion, stress response and immune response and the down-and-up group with 'fundamental cellular processes' such as DNA replication, mitosis, RNA splicing, DNA repair and translation initiation. Genes showing both dose-and time-dependent responses exhibited a mixture of both features. A highly non-linear relationship between the IR dose and the transcriptional relative response was obtained from the dose-dependent group. The time-dependent group also exhibited a non-linear relationship as the complex effect group did. Some of the early-reactive-phase (1-4 h) genes showed a differential expression response to 0.01, 0.05 and 0.2 Gy but were unresponsive to 1 Gy. Some of the late-recovery-phase (12-48 h) genes showed a differential expression to 1 Gy but were relatively unresponsive to other doses. We further characterized the gene expression patterns that could be implicated in the molecular mechanism of the cellular responses to low and very low-dose irradiation.</P>

Uncoupling Protein 3의 골격근 세포내 과발현이 OLETF 백서 및 배양된 골격근 세포에서 포도당대사에 미치는 영향

한정희,박혜선,고정민,김하영,강호경,이인규,박중열,홍성관,이재담,이기업 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:Uncoupling protein(UCP)는 미토콘드리아의 내막에 위치하는 단백질로 세포내의 과다한 에너지를 열로 발산시키는 기능을 가진다. 최근 동물의 갈색지방조직에만 존재하는 UCP와 유사성을 가진 아형들(UCP2,3)이 사람에게도 존재함이 알려져 큰 관심을 끌도 있는데 이중 UCP3는 그 발현이 골격근세포와 갈색지방조직에만 국한된다. 본 연구에서는 UCP3가 체내 인슐린 감수성을 결정하는데 가장 중요한 조직인 골격근에 국한되어 발현되는 점에 착안하여 UCP3를 골격근세포에 과발현시켰을 때 포도당 대사에 어떠한 영향이 나타나는 지를 조사하였다. 방법:25주령의 8마리의 OLETF 백서를 대상으로 하여 4마리는 골격근에 adenovirus 2mL(1×10¹²pfu/mL)를 주사하여 대조군으로 하였고 4마리는 골격근에 재조합법으로 제작된 adenovirus­UCP3 2mL(1×10¹²pfu/mL)를 주사하였다(UCP3 과발현군). UCP3를 투여한 백서에서 먹이섭취가 증가하는 경향이 있어 그 전날 대조군이 먹은 야의 먹이만큼 투여하였다. 골격근에 adenovirus를 주사한 10일 후에 euglycemic hyperinsulinemic clamp를 시행하였다. Adenovirus­UCP를 C2C12 골격근 세포에 transfection시켜 UCP3를 C2C12 골격근 세포에 transfection시켜 UPS3­C2C12를 만들고 C2C12 골격근 세포와 UPS3­C2C12 골격근 세포에서 포도당 수송 및 당원합성을 측정하였다. 결과:UCP3 과발현 OLETF에서 체중이 감소하는 경향을 보였고 인슐린 감수성이 증가하였다. C2C12세포에서 기저상태 포도당 수송은 1.28±0.17μmol/L/min였고 100nM 인슐린으로 2시간 처리한 후 2.67±0.20 μmol/L/min로 증가하였다. UCP3­C2C12 세포에서는 기저상태 포도당 수송이 3.98±0.13μmol/L/min로 증가되었고 인슐린 처리 후 5.74±0.44μmol/L/min로 증가하였다. 인슐린을 처리한 UCP3­C2C12 세포에 P13K 억제제인 wortmannin을 첨가하였을 때 포도당 수송활성이 3.81±0.20μmol/L/min로 감소하였다. 기저상태 당원합성은 C2C12 세포에서 0.25±0.01μmol/L/min였고 인슐린 처리 후 0.45±0.01μmol/L/min로 증가하였다. UCP3­C2C12 세포에서는 기저상태 당원합성이 0.62±0.01μmol/L/min였고 인슐린 처리 후 1.26±454μmol/L/min로 증가하였다. UCP3­C2C12세포에 wortmannin을 첨가하였을 때 당원합성율이 0.80±0.04μmol/L/min로 감소하였다. 결론:UCP3 과발현이 OLETF 백서에서 인슐린 감수성을 증가시켰고 골격근세포에서 포도당 수송 및 당원합성을 증가시켰다. wortmannin을 첨가하였을 때 포도당 수송 및 당원합성이 감소함으로 보아 이 과정이 인슐린 신호전달체계인 P13K에 일부 의존함을 알 수 있었다. Background : UC P3 is a mitochondrial membrane protein expressed selectively in the skeletal muscle and brown adipose tissue. Since the skeletal muscle is the main organ determining insulin sensitivity in the body, it was hypothesized that UCP3 overexpression in skeletal muscle cells would improve glucose metabolism. Methods : An adenovirus-UCP3 was produced by a recombinant DNA method. OLETF rats were divided into 2 groups. Four rats were injected with the adenovirus-UCP3 (UCP3 group) and others were injected with the adenovirus(control group) in the skeletal muscle. The UCP3 group was provided with the same quantity of food as that consumed by the control group on the previous day. Insulin sensitivity was evaluated by the euglycemic hyperinsulinemic clamp method. In a separate experiment, glucose transport and glycogen synthesis we evaluated in C2C212 cells transfected with ether an adenovirus or the adenovirus-UCP3. Results : The insulin sensitivity improved significantly and the body weight decreased in the UCP3 group. The glucose transport and glycogen synthesis were higher in the UCP3-C2C12 skeletal muscle cells at the basal state. After insulin treatment, glucose transport and glycogen synthesis were also higher in the UCP3-C2C12 cells but the increments were reduced after treatment with wortmannin, a PI3K inhibitor. Conclusion : Insulin sensitivity was higher in the UCP3-overexpressed OLETF rats in the in vivo study. UCP3 transfection also increased glucose transport and glycogen synthesis in the cultured skeletal muscle cells by a PI3K dependent mechanism(J Kor Diabetes Asso 25 :460~468, 2001).

췌장의 Retention Cyst

김미영,김영수,신용운,권계숙,조영업,김준미,김선후 대한소화기내시경학회 1996 Clinical Endoscopy Vol.16 No.6

A 38-year-old woman was admitted for evaluation of cyst-like lesion of the pancreas, which was incidentally found on physical check up. She has been well until the lesion was found except epigastric pain. On endoscopic retrograde pancreatogram, pancreatic head portion revealed a 2 x 2 cm sized cystic mass with marginal calcification. There was no definitive communication between pancreatic duct and cyatic mass, but amylase level of cystic content was increased. Surgical excision was performed, Microspically, the cystic wall showed flat lining epithelium with dense fibrosis and dysplastic calcification accompanied by chronic pancreatitis of adjacent pancreatic tissue.

Physiological and Protein Profiling Response to Drought Stress in KS141, a Korean Maize Inbred Line

김상곤,Hwan Hee Bae,Hwa Jin Jung,Jin-Seok Lee,Jung-Tae Kim,Tae Hoon Go,Beom-Young Son,Seong-Bum Baek,Young-Up Kwon,우미옥,Seonghyu Shin 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.4

Understanding the complex response mechanism of a crop to drought is the major step in the developing of tolerant genotypes. Inour study, to investigate physiological traits and proteome dynamics, an inbred maize (Zea mays L.) line (KS141) was subjected to10 days of water-withholding at the V5 or V6 leaf stage. The subsequent analysis of their physiological parameters revealed adecreased relative leaf water content, Fv/Fm, stomatal conductance, net CO2 assimilation rate, leaf transpiration, and water use efficiency,resulting in severe growth retardation of leaf area, stem length and width, aerial part, and root dry matter at 3 and 10 daysafter withholding water. However, aerial part and root dry matter were little changed during drought stress for 3 days. To understandthe proteome dynamics during the 10-day drought stress in maize leaves, comparative proteome analysis was carried out between thewell-watered and drought-treated leaves. Proteins were extracted using phenol extraction method from leaves with/without droughtstress, and then separated by 2-DE. After 2-DE gel analyses, 14 differentially expressed protein spots were identified by MALDITOFmass spectrometry. Out of 14, eleven and three protein spots were found to be up- or down-regulated, respectively. Interestingly, stress-related proteins such as glutathione S-transferase, abscisic stress-ripening proteins, and pathogenesis-related proteinswere increased by drought stress. Our study may provide molecular mechanisms and selective markers for drought tolerantmaize genotypes

野生담배(Nicotiana glauca) Callus의 生長과 葉綠素形成에 미치는 Pnytohormone, Sucrose 및 光度의 影響

呂邑東,蘇祥燮,金必榮 全北大學校 基礎科學硏究所 1989 基礎科學 Vol.12 No.1

To establish a basis of a photoautotrophic culture, effects of phytohormones, sucrose, and light intensity on growth and chlorophyll foundation of callus were investigated. The callus was induced from the leaf explants of a wild tobacco(Nicotiana glauca Graham) on the MS medium supplemented with 1.0 ㎎/ℓof 2,4-D. Growth of them was arrived at logistic phase for 16 days of culsure which exhibited growth rate of 187.5 mg per day, and arrived at early stationary phase for 28 days. Both NAA in auxins and kinetin in cytokinins known to be effective chlorophyll formation were used as phytohormone. Callus growth and chlorphyll formation were the most effective at the combination of 0.1 ㎎/ℓof NAA and 1.0 ㎎/ℓof kinetin. And chlorophyll a/b was increased according to the increase of the concentration of kinetin. Callus growth was enhanced according to the increase of the concentration of sucrose in the cases supplemented with 0.0, 0.5, 1.0, 2.0 % sucrose respectively to the above medium, but did not make a difference in the cases supplemented with 3.0% and 4.0% sucrose respectively to it. However, chlorophyll formation was not affected by the sucrose concentration in the medium but associated with growth rates of callus in media with various sucrose levels(r=0.89**). On the other hand, the increase in light intensity from 2,000 through 5,000 to 10,000ux stimulated callus growth and chlorophyll formation. Chlorophyll a/b was also enhanced according to the increase of light intensity. However, soluble protein contents of callus were decreased according to growth phase of callus.