자동 화학 분석기 ADVIA^(�) 1650의 평가

엄태현,조종래 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3

Objective : ADVIA?? 1650(Bayer Inc., NY, USA) is an automated chemistry analyzer introduced into Korea in 1999. We evaluated the performance of ADVIA 1650 for precision, linearity, recovery, and compared it with CX7 autoanalyzer(Bookman Instruments, Inc., CA. USA). Methods and Materials : We used pool serum and commercial qualify control materials, such as Control 1 and Control 2(Bayer Inc., NY, USA). A total of 22 test items, such as glucose, blood urea nitrogen(BUN), creatinine, uric acid, total protein, albumin, aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phsphatase(ALP), total bilirubin, choesterol, triglyceride(TG), phosphorus(P), magnesium(Mg), creatine kinase(CK), lactate dehydrogenase(LD). ?-glutamyl transferase(GGT), amylase, sodium(Na), potassium(K), and chloride(Cl) were evaluated. Results : Within-run coefficients of variations(CVs) of all test items except total bilirubin were less than 5%. Between-day CVs were less than 5% except creatinine, AST, ALT, total bilirubin. All test items showed good linearity with CVs higher than 0.99. Recovery rates were in the range of 90% to 106%. The correlations with CX7 were good with CVs higher than 0.97 in most of the test items, except creatinine, total protein and sodium, which showed CVs lower than 0.90. Conclusion : We think that ADVIA 1650 is a useful automatic chemistry anaylzer for high-volume laboratories.

한국인에서 ABO 혈액형과 균혈증의 관련성

엄태현,조종래 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3

Objectives: There have been debates on the relationship between ABO blood groups and infectious diseases. It is known that blood type A has the tendency to have staphylococcus aureus bacteremia. We investigated this relationship in Koreans. Methods and Materials: We analysed the ABO blood groups in the bacteremia patients in Ilsan Paik hospital, from January 2000 to March 2001. The number of patients with S. aureus bacteremia is 30, coagulase negative Staphylococcus(CNS) 40, Escherichia coli 21, and blood culture negatives(BN) 104. We used the distribution of ABO blood groups of 1,018 patients as the control, for whom ABO blood typings were done in blood bank during December 2000. Results: The frequencies of blood group A, B, O and AB in blood bank controls were 32%, 28%, 28%, and 13%, respectively. For S. aureus bacteremia, they were 20%, 43%, 20%, and 17%; for CNS, 45%, 35%, 15%, and 5%; for E. coli, 24%, 24%, 43%, and 10%, respectively. For BN, they were 35%, 27%, 23%, and 15%, respectively. There were no statistically significant differences in ABO blood groups between control and each bacteremia including BN. But there was the tendency that CNS group had higher frequency of blood type A and lower frequency of blood type O. Conclusions: We concluded that there is no significant relationship between ABO blood groups and bacteremia, but can not deny its presence because of the room for further studios.

Vitek Ⅱ에 의한 균동정 및 항균제감수성 결과에 균주보관시간이 미치는 영향

조종래,엄태현 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3

Objective : It would be frequent that routine tests in clinical microbiology laboratory are interrupted more than 2 days because of the five-day workweek legislation, which is thought to be launched near future in Korea. We investigated the impacts of the extended storage of bacterial colonies in secondary culture media on the bacterial identification and antibiotic susceptibility tests. Methods and Materials : Five strains of Staphylococcus aureus, 4 coagulase negative staohylococcus(CNS), 3 Escherichia coli. 4 Pseudomonas aeruginosa, and 4 Acineto-bacter baumannii, which are frequently isolated from clinical samples in Ilsan Paik Hospital were used for this study. We did the identification and antibiotic susceptibility tests by VITEK 2 system, using colonies stored for 2 days, 4 days, and 6 days in room temperature. Fesults : There were no changes in identification until 6 day storage. The percentages of the organism-antibiotic combinations in which minimum inhibitory concentration(MIC) changed more than 1 dilution were as follows; S. aureus 9.0%, CNS 17.5%, E. coli 10.0%, P. aeruginosa 23.7%, and A. baumannii 22.4%. The percentages with more than 2 dilution changes, which are considered to be significant, were as follows: S. aureus 0%, CNS 3.8%, 2. coli 1.7%. P. aeruginosa 5.3%, and A. baumannii 6.6%. The very major errors observed in S. aureus, CNS, E. coli. P. aeruginosa and A. baumannnii were 0%, 1.3%, 1.7%, 0% and 2.6%, respectively; for minor errors, 1.0%, 0%, 1.7%, 6.6% and 6.6%, respectively. Major errors were not observed in any of the organism-antibiotic combinations studied. Conclusion : We thought that it would be acceptable for clinical microbiology laboratory to delay the antibiotic susceptibility tests during holidays.

간경변증 환자에서 Vibrio cholera non-01, non-0139에 의한 균혈증

조종래,엄태현 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3

We report a case of Vibrio cholerae septicemia in a 64-year-old male patient with liver cirrhosis who complained of fever, diarrhea, and abdominal pain upon admission. The day before the onset of symptoms, he had had seafood. His blood culture was positive for a non-cholera toxin-producing non-O1, non-O139 V. cholerae. The organism was not cultured in the patient's stool specimen, probably because of the empiric antibiotics treatment before blood collection. He recovered by antibiotics, cefotaxime and ciprofloxacin.

특발성 과호산구 증후군 2예

김은순,엄태현,김준희,신보문,김예희 인제대학교 1999 仁濟醫學 Vol.20 No.1S

특발성 과호산구 증후군은 지속적인 호산구의 과도 생산으로 여러 장기에 손상을 가져오는 백혈구 증가 질환이다. 가장 많이 침범 되는 장기인 심장의 침범여부가 이환율과 생존율의 중요한 요인이다. 폐 침범은 약 40%에서 나타나며 만성, 지속성, 비객담성 기침이 주된 증상이다. 치료는 스테로이드를 투여하는 것인데 스테로이드에 반응하지 않은 경우 세포 독성 약제를 이차적으로 사용할 수 있다. 저자들은 폐를 침범한 특발성 과호산구성 증후군에서 스테로이드에 반응하지 않아 세포독성 약제 및 면역 억제제로 증상의 호전이 있었던 1예와 심장을 침범한 특발성 과호산구성 증후군으로 스테로이드에 반응한 1예를 경험하였기에 보고하는 바이다. The idiopathic hypereosinophilic syndrome is a leukoproliferative disorder marked by a sustained overproduction of eosinophilia with evidence of organ involvement. In patients with organ involvement, initial therapy is prednisone. Cytotoxic agents have been used in patients who are not responsive to corticosteroid. We experienced a 40-year-old woman with lung in volvement who was treated with cytarabine, azathioprine, prednisolone and a 46-year-old man with heart failure who showed partial response.

Morganella Morganii Sepsis with Massive Hemolysis

Tae Hyun Um,Eu Suk Kim,Jong Hoon Kim,Chong Rae Cho,Ji Yoon Rhu,Hye Ran Lee,Jae Won Jeong 대한의학회 2007 Journal of Korean medical science Vol.22 No.6

Evaluation of Adequacy for Transfusion Practice and Meaning of Recent Amendment of Blood Management Law

Tae-Hyun Um 대한외과학회 2020 대한외과학회 학술대회 초록집 Vol.2020 No.11

Comparison of HPV Genotyping Assays and Hybrid Capture 2 for Detection of High-Risk HPV in Cervical Specimens.

Um, Tae Hyun,Lee, Eun Hee,Chi, Hyun-Sook,Kim, Jong-Won,Hong, Young-Joon,Cha, Young Joo Institute for Clinical Science] 2011 Annals of clinical and laboratory science Vol.41 No.1

<P>High-risk types of human papillomavirus (HR-HPV) are among the primary causes of cervical cancers. Hybrid Capture 2 (HC-II) (Digene, Gaithersburg, MD), which detects 13 HR-HPVs as a group, is the only HPV assay approved to date by the United States Food and Drug Administration. In Korea, several HPV genotyping assays are commercially available, including HPV RFMP (GeneMatrix Co., Seoul), HPVDNACHIP (Biomedlab Co., Seoul), and MyHPV Chip (Mygene Co., Seoul). We compared the results of these assays with those of HC-II. Among 553 residual samples of liquid-based Pap tests, a total of 435 (78.7%) were available for HPV assays. They were classified into four cytologic categories: normal, atypical squamous cells of undetermined significance (ASCUS), low-grade cervical squamous intraepithelial lesions (LSIL), and high grade SIL or carcinoma (HSIL+). Among these samples, 23.0%, 40.6%, 82.5%, 93.8% were HR-HPV positive by HC-II, respectively; 6.6%, 18.1%, 44.4%, 84.4%, by HPV RFMP, respectively; 5.7%, 24.5%, 54.0%, 84.4%, by HPVDNACHIP, respectively; and 6.6%, 11.6%, 42.9%, 84.4%, by MyHPV, respectively. Compared with HC-II, the concordance rates and kappa values were 70.6% and 0.421 for HPV RFMP; 75.4% and 0.514 for HPVDNACHIP; and 67.8% and 0.367 for MyHPV. The concordance rates and kappa values between genotyping assays were 85.1% and 0.644 for HPV RFMP and HPVDNACHIP; 83.4% and 0.574 for HPV RFMP and MyHPV Chip; and 82.8% and 0.579 for HPVDNACHIP and MyHPV Chip. In conclusion, compared with HC-II test, the genotyping tests showed more than fair concordance but lower sensitivity in the detection of HR-HPVs, limiting their usefulness as HR-HPV screening tools.</P>

Introduction of Patient Blood Management (PBM) and Implementation Plan in Korea

Tae Hyun Um 대한외과학회 2018 대한외과학회 학술대회 초록집 Vol.2018 No.11

Nocturia and Sleep: Focus on Common Comorbidities and Their Association with Obstructive Sleep Apnea

Um Yoo Hyun,Oh Ji-Hye,Kim Tae Won,서호준,Kim Sung-Min,Chung Jun-Soo,Jeong Jong-Hyun,Hong Seung-Chul 대한수면학회 2020 sleep medicine research Vol.11 No.2

Nocturia is a frequently encountered complaint in patients visiting sleep clinics. Nocturia and its detrimental effect on sleep continuity and quality have been frequently discussed in previous studies. Moreover, abundant research has been conducted to delineate the close relationship between nocturia and the pathophysiology of obstructive sleep apnea (OSA). In this review, we discuss the common comorbidities that can provoke nocturia and their relationship with OSA and sleep.