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- 저자 : Uchizono (검색결과 5 건)
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Deciphering the Genes for Taste Receptors for Fructose in Drosophila
Uchizono, Shun,Itoh, Taichi Q.,Kim, Haein,Hamada, Naoki,Kwon, Jae Young,Tanimura, Teiichi Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.10
Taste sensitivity to sugars plays an essential role in the initiation of feeding behavior. In Drosophila melanogaster, recent studies have identified several gustatory receptor (Gr) genes required for sensing sweet compounds. However, it is as yet undetermined how these GRs function as taste receptors tuned to a wide range of sugars. Among sugars, fructose has been suggested to be detected by a distinct receptor from other sugars. While GR43A has been reported to sense fructose in the brain, it is not expressed in labellar gustatory receptor neurons that show taste response to fructose. In contrast, the Gr64a-Gr64f gene cluster was recently shown to be associated with fructose sensitivity. Here we sought to decipher the genes required for fructose response among Gr64a-Gr64f genes. Unexpectedly, the qPCR analyses for these genes show that labellar expression levels of Gr64d and Gr64e are higher in fructose low-sensitivity flies than in high-sensitivity flies. Moreover, gustatory nerve responses to fructose in labellar sensilla are higher in Gr64d and Gr64f mutant lines than in mutant flies of the other Gr64a-Gr64f genes. These data suggest the possibility that deletion of GR64D or GR64F may indirectly induce enhanced fructose sensitivity in the labellum. Finally, we conclude that response to fructose cannot be explained by a single one of the Gr64a-Gr64f genes.
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Deciphering the Genes for Taste Receptors for Fructose in Drosophila
Shun Uchizono,Taichi Q. Itoh,김해인,Naoki Hamada,권재영,Teiichi Tanimura 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.10
Taste sensitivity to sugars plays an essential role in the initiation of feeding behavior. In Drosophila melanogaster, recent studies have identified several gustatory receptor (Gr) genes required for sensing sweet compounds. However, it is as yet undetermined how these GRs function as taste receptors tuned to a wide range of sugars. Among sugars, fructose has been suggested to be detected by a distinct receptor from other sugars. While GR43A has been reported to sense fructose in the brain, it is not expressed in labellar gustatory receptor neurons that show taste response to fructose. In contrast, the Gr64a–Gr64f gene cluster was recently shown to be associated with fructose sensitivity. Here we sought to decipher the genes required for fructose response among Gr64a–Gr64f genes. Unexpectedly, the qPCR analyses for these genes show that labellar expression levels of Gr64d and Gr64e are higher in fructose low-sensitivity flies than in high-sensitivity flies. Moreover, gustatory nerve responses to fructose in labellar sensilla are higher in Gr64d and Gr64f mutant lines than in mutant flies of the other Gr64a–Gr64f genes. These data suggest the possibility that deletion of GR64D or GR64F may indirectly induce enhanced fructose sensitivity in the labellum. Finally, we conclude that response to fructose cannot be explained by a single one of the Gr64a–Gr64f genes.
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안미자,허선정,김은현,이승훈,신준섭,김묘경,James A. Uchizono,황완균,김동석 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.4
In this study, we isolated scopoletin from Cirsium setidens Nakai (Compositae) and tested its effectson melanogenesis. Scopoletin was not toxic to cells at concentrations less than 50 μM and increasedmelanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulatedthe total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however,scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator oftyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production ofmicrophthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP responseelement-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results,preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.
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Ahn, Mi-Ja,Hur, Sun-Jung,Kim, Eun-Hyun,Lee, Seung Hoon,Shin, Jun Seob,Kim, Myo-Kyoung,Uchizono, James A.,Whang, Wan-Kyunn,Kim, Dong-Seok The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.4
In this study, we isolated scopoletin from Cirsium setidens Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than $50{\mu}M$ and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.
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Mi-Ja Ahn,Sun-Jung Hur,Eun-Hyun Kim,Seung Hoon Lee,Jun Seob Shin,Myo-Kyoung Kim,James A. Uchizono,Wan-Kyunn Whang,Dong-Seok Kim 대한생리학회-대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.4
In this study, we isolated scopoletin from <em>Cirsium setidens</em> Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than 50 μM and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.
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