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Self-association of bee propolis: effects on pharmaceutical applications
Jiri Trousil,Martin Hruby,Jiri Panek,Jana Matějková,Jan Kucka,Petr Stepanek 한국약제학회 2014 Journal of Pharmaceutical Investigation Vol.44 No.1
Propolis is a resinous product collected byhoneybees from various plant sources; it is widely used intraditional medicine and has been reported to have a broadspectrum of pharmacological effects (i.e., antibacterial,antifungal, antiviral and anti-inflammatory effects). Themost commonly used propolis formulations are gargles, inwhich propolis tinctures are diluted with water. The dilutionprocess is accompanied by nanoprecipitation, and thepropolis droplets are dispersed in the prepared gargle. Inthe present study, we investigated the dependence of theproperties of propolis nanodispersions on the method ofpreparation. The particle size was found to be approximately150 nm and was observed to decrease withincreasing dilution as the zeta potential of the particlesbecame more negative, which stabilized the dispersion. The dispersion dissolved upon alkalization and reprecipitatedduring acidification. The addition of salt destabilizedthe dispersion. The uptake of propolis from the dispersionwas modeled using 1-octanol and was found to be rapidand only slightly dependent on the nanoparticle size. Propolis susceptibility tests showed that the most effectivedispersion of propolis was tenfold-diluted EEP (P-80-10and JP-80-10). The disc diffusion method was used toevaluate the antibacterial activity of chosen dispersions ofpropolis against Staphylococcus aureus, Escherichia coliand Candida albicans. Propolis samples with differentlocations of origin exhibited different effects against thestrain of C. albicans.
Antimicrobial polyhydroxybutyrate submicron fiber mat loaded with extract of Hypericum perforatum
Milos Beran,Ales Horna,Viktor Vorisek,Eliska Berkova,Radka Korinkova,Vojtech Trousil,Marketa Hrubanova 한국식물생명공학회 2022 JOURNAL OF PLANT BIOTECHNOLOGY Vol.49 No.3
The aim of this work was to prepare a new biodegradable polyhydroxybutyrate (PHB) submicron fiber mat loaded with hypericin-rich Hypericum perforatum raw extract by centrifugal spinning technology, an alternative approach to the traditional method of electrospinning to fabricate nanofibers or microfibers from solutions at high speed and low cost. Hypericins in methanol/acetone extract of H. perforatum were determined by UHPLC-MS/MS and HPLC/PDA. Submicron fiber mats composed of pure PHB or PHB enriched with H. perforatum extract were prepared using a pilot plant demonstrator for the centrifugal spinning technology and characterized by SEM. Singlet oxygen production was quantified by the 1,3-diphenylisobenzofuran (DPIBF) method in hexane. The results proved a significant production of singlet oxygen by the prepared submicron fiber mat. We also found a significant antibacterial activity against the bacterial strain Escherichia coli CCM 5417 by a method in accordance with JIS Z 2801/ISO 22196 standards. The H. perforatum extract-enriched PHB submicron fiber mats showed potential for the development of self-cleaning and antimicrobial air filters.