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- 저자 : Torio (검색결과 2 건)
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Torio, Herena,Luck, Kirsten Sustainable Building Research Center 2011 International journal of sustainable building tech Vol.2 No.2
Due to the low temperature level demanded for space heating and domestic hot water supply in buildings exergy demands for these energy uses are low. However, these demands are often met by high grade energy sources, such as fossil fuels or electricity which could instead be used for other higher-grade applications (e.g. power production). The use of waste heat with low exergy content allows a good matching between the exergy level of the demand and supply sides and represents, thus, a very efficient manner of supplying thermal energy demands in buildings. Similarly, balanced ventilation systems with efficient heat recovery units allow supplying a great part of the space heating demand with low temperature heat recovered from the exhaust air. Here, two different supply options are considered for a small neighborhood: district heating with and without balanced ventilation system with heat recovery. The paper shows the appropriate use of these systems.
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Sequential Differentiation of Human Bone Marrow Stromal Cells for Bone Regeneration
Eva Johanna Huebner,Nestor Torio Padron,David Kubosch,Guenter Finkenzeller,Norbert P. Suedkamp,Philipp Niemeyer 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.5
In this study we hypothesized that as a simulation of endochondral bone formation, bone marrow stromal cell (BMSC) provide a sequential chondro-osteogenic differentiation potential. A chondrogenic priming of BMSC leads to a spontaneous three-dimensional cell formation. BMSC were chondrogenically differentiated prior to an osteogenic stimulation. Duration of cell culture was 28 days, whereas in group A BMSC were chondrogenically differentiated for 1 day, followed by an osteogenic differentiation for 27 days. In group B BMSC were chondrogenically differentiated for 14 days prior to an osteogenic differentiation of 14 days and group C BMSC were differentiated chondrogenically for 28 days serving as a chondrogenic control group. Chondrogenic priming induced a spontaneous three-dimensional cell formation. To survey the stability of the osteogenic phenotype in the absence of an osteogenic stimulus, investigations were performed in vivo in a specially adapted chorioallantoic membrane model of fertilized White Leghorn eggs. Histology and real time polymerase chain reaction revealed a higher amount of osteogenic extracellular matrix synthesis and significant higher expressions of osteogenic marker genes in group B after 14 days of chondrogenic and 14 days of osteogenic stimulation. Matrix calcification in vivo in the absence of an osteogenic stimulus could be demonstrated. The results of the present study support the theory of a sequential differentiation potential of BMSC. A chondrogenic priming of BMSC stimulated into the osteogenic lineage result in a stable osteogenic phenotype in a scaffold-free, three-dimensional tissue engineering application.
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