http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Double-Face Activity of Resveratrol in Voluntary Runners: Assessment of DNA Damage by Comet Assay
Barbara Tomasello,Salvatore Grasso,Giuseppe Malfa,Stefania Stella,Marco Favetta,Marcella Renis 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.5
Voluntary runners are subjected to a massive increase in reactive oxygen/nitrogen species production, which can promote different oxidative stress–related diseases such as premature aging, neurodegenerative disorders, and cancer. The aims of this work were to evaluate the following in peripheral blood cells of voluntary runners: (i) DNA status; (ii)susceptibility to the in vitro insult induced by hydrogen peroxide (H2O2) as a breaking agent; (iii) capabilities of 3,5,40-trihydroxystilbene (RESV) in counteracting DNA damage. Twenty-five male voluntary runners were compared with 20sedentary men, as age-matched controls, and DNA status was evaluated with different versions of comet assay: alkaline,neutral, and Fpg enzyme–modified version to measure 8-OH-deoxyguanosine (8-oxo-dG) levels. The H2O2 and/or RESV treatments were performed directly on agarose-embedded cells (atypical comet assay). The results evidenced DNA damage and levels of 8-oxo-dG higher in runners than in sedentary control subjects. The runners’ DNA was more prone to the in vitro–induced oxidative insult (200 lM H2O2) than that of the control group. Resveratrol (100 lM), depending on the individual basal DNA status, was able to switch from antioxidant to pro-oxidant. Our results, on the one hand, validated the proposed in vitro experimental protocol in order to measure individual DNA status. On the other hand, our data point out the importance of monitoring the athletes’ redox status before subjecting them to dietary supplementation treatment.
Maurizio Bellavia,Giuseppe Damiano,Vincenzo Davide Palumbo,Gabriele Spinelli,Giovanni Tomasello,Antonio Marrazzo,Silvia Ficarella,Antonio Bruno,Antonino Sammartano,Tiziana Fiorentini,Antonio Scio,Caro 한국유방암학회 2012 Journal of breast cancer Vol.15 No.3
Granulomatous mastitis is a rare benign inflammatory disease of the breast with multiple etiologies such as tuberculosis, sarcoidosis, foreign body reaction, and mycotic and parasitic infections. In contrast, idiopathic granulomatous mastitis (IGM) is characterized by the presence of chronic granulomatous lobulitis in the absence of an obvious etiology. Clinically and radiologically it may mimic breast carcinoma and so awareness of surgeons,pathologists, and radiologists is essential to avoid unnecessary mastectomies. Cases of IGM are reported during antidepressant therapy in patients also showing high levels of prolactinemia. In these cases, we believe that surgical excision must be avoided being replaced with a conservative management of the pathological condition based on a corticosteroid treatment.