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In vitro morphogenesis in Selaginella microphylla (Kunth.) Spring
Timir Baran Jha,Siddhartha Mukherjee,Anusuya Basak,Jukta Adhikari 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
Selaginella, an extant genus of primitivevascular plants, has survived over 400 million years ofevolution. In vitro morphogenesis in Selaginella microphyllais considered for the first time to establish a welldocumentedaseptic culture on half- strength Murashigeand Skoog’s basal medium with 2ip (4.92–49.21 lM), orKn (4.65–46.47 lM) or GA3 (2.89–28.90 lM) for shootmultiplication, and with different concentrations of IBA(4.9–49 lm) to initiate root cultures. GA3 was instrumentalfor shoot multiplication as well as induction of reproductivestructures in each and every leaf axil. On the otherhand, it is observed that IBA alone in S. microphylla canact as signal molecules for induction of enormous numbersof root masses from a few existing roots. An interestingpattern of re-differentiation has also been observed whereapical portions of large numbers of roots were converted togreen shoot apical meristems. Further differentiation producedtiny green shoots. Distinct bipolarity was noted inshoots when they were isolated from root masses andappeared as embryo-like structures. Chromosome analysisfrom in vitro sporophytic plants revealed 2n = 16 chromosomes,indicating chromosomal stability. The interesting invitro pattern of morphogenesis obtained in S. microphyllamay provide new insights into totipotency of plants.
In vitro morphogenesis in Selaginella microphylla (Kunth.) Spring
Jha, Timir Baran,Mukherjee, Siddhartha,Basak, Anusuya,Adhikari, Jukta 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
Selaginella, an extant genus of primitive vascular plants, has survived over 400 million years of evolution. In vitro morphogenesis in Selaginella microphylla is considered for the first time to establish a well-documented aseptic culture on half- strength Murashige and Skoog's basal medium with 2ip ($4.92-49.21{\mu}M$), or Kn ($4.65-46.47{\mu}M$) or $GA_3$ ($2.89-28.90{\mu}M$) for shoot multiplication, and with different concentrations of IBA (4.9-49 lm) to initiate root cultures. $GA_3$ was instrumental for shoot multiplication as well as induction of reproductive structures in each and every leaf axil. On the other hand, it is observed that IBA alone in S. microphylla can act as signal molecules for induction of enormous numbers of root masses from a few existing roots. An interesting pattern of re-differentiation has also been observed where apical portions of large numbers of roots were converted to green shoot apical meristems. Further differentiation produced tiny green shoots. Distinct bipolarity was noted in shoots when they were isolated from root masses and appeared as embryo-like structures. Chromosome analysis from in vitro sporophytic plants revealed 2n = 16 chromosomes, indicating chromosomal stability. The interesting in vitro pattern of morphogenesis obtained in S. microphylla may provide new insights into totipotency of plants.
Performance Prediction of Vertical Submersible Centrifugal Slurry Pump
Lal Gopal Das,Prasanta Kumar Sen,Timir Kanti Saha,Chanda, Arunabha 한국유체기계학회 2010 International journal of fluid machinery and syste Vol.3 No.2
Performance prediction methodology for centrifugal submersible slurry pump has been presented in this paper. An in-depth study on various energy-head losses occurring through the pump flow in rotating reference frame has been carried out in this research work. Head-flow characteristics of the centrifugal pump have been accomplished in two stages. First performance of the centrifugal pump with clear water has been predicted by analyzing and deducting head losses from the theoretical head. Effects of solid particles size, specific gravity and concentration on pump slurry flow have been investigated. Additional head losses due to solid particles in the slurry have been predicted, analyzed and then deducted from clear water head to establish the performance of centrifugal slurry pump. The performance of centrifugal slurry pump has been predicted at with accuracy of 88 to 90 % for solid concentration of 18 % to 5 % by volume.
Jatropha curcas: a review on biotechnological status and challenges
Mukherjee, Priyanka,Varshney, Alok,Johnson, T. Sudhakar,Jha, Timir Baran The Korean Society of Plant Biotechnology 2011 Plant biotechnology reports Vol.5 No.3
Plant tissue culture and molecular biology techniques are powerful tools of biotechnology that can complement conventional breeding, expedite crop improvement and meet the demand for availability of uniform clones in large numbers. Jatropha curcas Linn., a non-edible, eco-friendly, non-toxic, biodegradable fuel-producing plant has attracted worldwide attention as an alternate sustainable energy source for the future. This review presents a consolidated account of biotechnological interventions made in J. curcas over the decades and focuses on contemporary information and trends of future research.
Saxena, Amit,Sharma, Abha,Singh, Beer,Suryanarayana, Malladi Venkata Satya,Mahato, Timir Haran,Sharma, Mamta,Semwal, Rajendra Prasad,Gupta, Arvind Kumar,Sekhar, Krishnamurthy Korean Carbon Society 2005 Carbon Letters Vol.6 No.3
Room temperature kinetics of degradation of nerve agent simulants and sarin, an actual nerve agent at the surface of different carbon based adsorbent materials such as active carbon grade 80 CTC, modified whetlerite containing 2.0 and 4.0 % NaOH, active carbon with 4.0 % NaOH, active carbon with 10.0 % Cu (II) ethylenediamine and active carbon with 10.0 % Cu (II) 1,1,1,5,5,5-hexafluoroacetylacetonate were studied. The used adsorbent materials were characterized for surface area and micropore volume by $N_2$ BET. For degradation studies solution of simulants of nerve agent such as dimethyl methylphosphonate (DMMP), diethyl chlorophosphate (DEClP), diethyl cyanophosphate (DECnP) and nerve agent, i.e., sarin in chloroform were prepared and used for the uniform adsorption on the adsorbent systems using their incipient volume at room temperature. Degradation kinetics was monitored by GC/FID and was found to be following pseudo first order reaction. Kinetics parameters such as rate constant and half life were calculated. Half life of degradation with modified whetlerite (MWh/NaOH) system having 4.0 % NaOH was found to be 1.5, 7.9, 1206 and 20 minutes for DECnP, DEClP, DMMP and sarin respectively. MWh/NaOH system showed maximum degradation of simulants of nerve agents and sarin to their hydrolysis products. The reaction products were characterized using NMR technique. MWh/NaOH adsorbent was also found to be active against sulphur mustard.