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Bohidar, Sagarika,Pattanaik, Suchismita,Thiruvanoukkarasu, Manikkannan 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
A rapid and efficient in vitro propagation protocol by enhanced multiple shoot proliferation from internode cultures of Ruta graveolens was established. Mean shoot number was maximum (55.83) in Murashige and Skoog (MS) basal medium fortified with $1.0mgL^{-1}$ benzyl amino purine and $0.25mgL^{-1}$ indole-3-acetic acid. The elongated shoots rooted within 10-12 days in 1/2-strength MS medium supplemented with $2.0mgL^{-1}$ indole 3-butyric acid. About 80 % of the rooted plantlets survived acclimatization and transfer to the field. Phytochemical analysis revealed that micropropagated plants produced linear furanocoumarins, characteristic of the species, in greater quantities as compared to the in vivogrown plants. The results will facilitate the conservation of this valuable medicinal plant and to obtain plants with improved phytochemical constituents.
Sagarika Bohidar,Suchismita Pattanaik,Manikkannan Thiruvanoukkarasu 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
A rapid and efficient in vitro propagation protocolby enhanced multiple shoot proliferation from internodecultures of Ruta graveolens was established. Meanshoot number was maximum (55.83) in Murashige andSkoog (MS) basal medium fortified with 1.0 mg L-1benzyl amino purine and 0.25 mg L-1 indole-3-acetic acid. The elongated shoots rooted within 10–12 days in 1/2-strength MS medium supplemented with 2.0 mg L-1indole 3-butyric acid. About 80 % of the rooted plantletssurvived acclimatization and transfer to the field. Phytochemicalanalysis revealed that micropropagated plantsproduced linear furanocoumarins, characteristic of thespecies, in greater quantities as compared to the in vivogrownplants. The results will facilitate the conservation ofthis valuable medicinal plant and to obtain plants withimproved phytochemical constituents.