Is Hearing Loss in Infants Associated With Risk Factors? Evaluation of the Frequency of Risk Factors

Çiǧdem Tepe Karaca,Çaǧatay Oysu,Sema Zer Toros,Barıș Naiboǧlu,Ayșegül Verim 대한이비인후과학회 2014 Clinical and Experimental Otorhinolaryngology Vol.7 No.4

Objectives. To evaluate the frequency of risk factors and their influence on the evoked otoacoustic emission (OAE) of in- fants. Methods. All newborns between November 2009 and June 2012 in Haydarpaşa Numune Education and Research Hospi- tal were tested on distortion evoked OAE screening test. Total of 2,284 infants were examined. Sex, maternal infec- tious disease, birth type (vaginal birth or caesarean sectio), birth weight, familial hearing loss, intermarriage of par- ents, hyperbilirubinemia, intensive care were analyzed as risk factors. Results. Total of 2,284 neonates were screened (1,220 males and 1,064 females) for the presence of OAE in both ears. Vagi- nal delivery, maternal infections during pregnancy, intermarriage of parents relative, low birth weight(<1,500 g) are related risk factors to failure of screening with OAE in our study. There was no statistically significant difference in sex ratios, birth weight, familial hearing loss, hyperbilirubinemia, and intensive care stay. Conclusion. Risk factors are only as useful as their predictive power. Not enough is known about which risk factors are rel- evant, which babies have the risk factors, or which babies will fail to attend follow-up, the effectiveness of targeted hearing loss testing is questionable at this point in time. A system needs to be developed to clarify which risk factors are discoverable, predictive and useful.

Determination of In Vitro Antioxidative and Antimicrobial Properties and Total Phenolic Contents of Ziziphora clinopodioides, Cyclotrichium niveum, and Mentha longifolia ssp. typhoides var. typhoides

Gursoy, Nevcihan,Sihoglu-Tepe, Arzuhan,Tepe, Bektas The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.3

The aerial parts of Ziziphora clinopodioides, Cyclotrichium niveum, and Mentha longifolia ssp. typhoides var. typhoides were screened for their possible antioxidant and antimicrobial activities in addition to their penolic contents. Antioxidant activity was employed by two complementary test systems: 2,2'-diphenyl-1-picrylhydrazyl free radical scavenging and $\beta$-carotene/linoleic acid. In the first case, Z. clinopodioides was superior to the other species with a 50% inhibitory concentration value of $37.73\;{\pm}\;1.18\;{\mu}g/mg$. Similar results were obtained from the b-carotene/linoleic acid system. Inhibition capacity of the linoleic acid of Z. clinopodioides was $83.56\;{\pm}\;1.19%$. Additionally, antioxidant activities of butylated hydroxytoluene, curcumin, and ascorbic acid were determined in parallel experiments. Methanol extracts obtained from the plants studied were found to have moderate antimicrobial activity against all microorganisms tested. In general, Z. clinopodioides extract exhibited stronger activity than the other extracts. On the other hand, Acinetobacter lwoffii and Candida krusei were the most sensitive microorganisms for the all extracts. The amount of the total phenolics was highest in Z. clinopodioides extract ($129.55\;{\pm}\;2.26\;{\mu}g/mg$), followed by M. longifolia ssp. typhoides var. typhoides ($93.47\;{\pm}\;1.84\;{\mu}g/mg$). It is extremely important to note that there is a positive correlation between antioxidant activity potential and amount of phenolic compounds.

Determination of In Vitro Antioxidative and Antimicrobial Properties and Total Phenolic Contents of Ziziphora clinopodioides, Cyclotrichium niveum, and Mentha longifolia ssp. typhoides var. typhoides

Nevcihan Gursoy,Arzuhan Sihoglu-Tepe,Bektas Tepe 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.3

The aerial parts of Ziziphora clinopodioides, Cyclotrichium niveum, and Mentha longifolia ssp. typhoides var. typhoides were screened for their possible antioxidant and antimicrobial activities in addition to their penolic contents. Antioxidant activity was employed by two complementary test systems: 2,2′-diphenyl-1-picrylhydrazyl free radical scavenging and β-carotene/linoleic acid. In the first case, Z. clinopodioides was superior to the other species with a 50% inhibitory concentration value of 37.73±1.18μg/mg. Similar results were obtained from the β-carotene/linoleic acid system. Inhibition capacity of the linoleic acid of Z. clinopodioides was 83.56±1.19%. Additionally, antioxidant activities of butylated hydroxytoluene, curcumin, and ascorbic acid were determined in parallel experiments. Methanol extracts obtained from the plants studied were found to have moderate antimicrobial activity against all microorganisms tested. In general, Z. clinopodioides extract exhibited stronger activity than the other extracts. On the other hand, Acinetobacter lwoffii and Candida krusei were the most sensitive microorganisms for the all extracts. The amount of the total phenolics was highest in Z. clinopodioides extract (129.55±2.26μg/mg), followed by M. longifolia ssp. typhoides var. typhoides (93.47±1.84μg/mg). It is extremely important to note that there is a positive correlation between antioxidant activity potential and amount of phenolic compounds.

Use of sugammadex in Rett syndrome: A case report

Kupeli, Ilke,Tepe, Emine,Kuyrukluyildiz, Ufuk The Korean Dental Society of Anesthsiology 2018 Journal of Dental Anesthesia and Pain Medicine Vol.18 No.4

Rett syndrome (RS) is a neurodevelopmental disorder characterized by loss of cognitive, motor, and social skills, epilepsy, autistic behavior, abnormal airway patterns, gastroesophageal reflux, nutritional problems, and severe scoliosis. Although girls with RS show normal or near-normal growth until 6-8 months, they lose their skills after that. The anesthetic management of these patients requires care because of all these clinical features. Especially in the postoperative period, prolonged apnea is common and extubation is delayed. In this case report, the effect of using sugammadex was presented in a 16-year-old girl with RS. The patient's all bimaxillary teeth and 4 wisdom teeth were extracted under general anesthesia in one session with minimal surgical trauma and moderate bleeding. Sugammadex can be a rapid and reliable agent for the reversal of the neuromuscular block in neurodegenerative patients.

Antitumoral Effects of Melissa officinalis on Breast Cancer in Vitro and in Vivo

Saraydin, Serpil Unver,Tuncer, Ersin,Tepe, Bektas,Karadayi, Sule,Ozer, Hatice,Sen, Metin,Karadayi, Kursat,Inan, Deniz,Elagoz, Sahande,Polat, Zubeyde,Duman, Mustafa,Turan, Mustafa Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Background: There is a long standing interest in the identification of medicinal plants and derived natural products for developing cancer therapeutics. Here we investigated the antiproliferative properties of Melissa officinalis (MO) from Turkey on breast cancer. Methods: MO extracts were studied for cytotoxicity against breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). In vitro apoptosis studies were performed by annexin V staining and flow cytometry analyses. Immunohistochemistry for Ki-67 and caspase 7 in the tumoral tissue sections of DMBA-induced mammary tumors in rats was also performed, along with TUNEL assays to detect apoptotic cells. In vivo anticancer activity testing was carried out with reference to inhibition of growth of DMBA induced mammary tumors in rats. Results: MO showed cytotoxicity against three cancer cell lines, inducing increase in Annexin-positive cells. Expression of caspase-7 protein and TUNEL positive cells were much higher in rats treated by MO, compared with the untreated control group, while expression of Ki-67 was decreased. Furthermore, in vivo studies showed that mean tumor volume inhibition ratio in MO treated group was 40% compared with the untreated rats. Conclusion: These results indicated that MO extrcts have antitumoral potential against breast cancer.

Chemical Composition, Antioxidant, and Enzyme Inhibitory Activities of the Essential Oils of Three Phlomis Species as well as Their Fatty Acid Compositions

Cengiz Sarikurkcu,Mehmet Cemil Uren,Mehmet Sefa Kocak,Mustafa Cengiz,Bektas Tepe 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3

This study aimed to investigate the chemical composition, antioxidant, and enzyme inhibitory activities of the essential oils of Phlomis armeniaca WILLD., P. nissolii L., and P. pungens WILLD. var. pungens as well as their fatty acid contents. Germacrene D was found as the major compound in the oils (24.7, 15.1, and 7.2%, respectively). Additionally, n-hexadecanoic acid, hexahydrofarnesyl acetone, β-caryophyllene, and linalool were the other main compounds in the oils. Among the fatty acids, C18:3 ω3 and C18:2 ω6 were determined in high quantities in P. armeniaca (23.14 and 18.01%, respectively) and P. pungens var. pungens (24.64 and 17.51%, respectively). The essential oils of P. armeniaca and P. pungens var. pungens showed remarkable antioxidant and metal chelating activities as well as great reducing power potentials. These oils also showed a moderate scavenging effect on ABTS radicals. The oils also exhibited various degrees of inhibitory activities on AChE, BChE, α-amylase, α-glucosidase, and tyrosinase.

Association between Laryngeal Squamous Cell Carcinoma and Polymorphisms in Tumor Necrosis Factor Related Apoptosis Induce Ligand (TRAIL), TRAIL Receptor and sTRAIL Levels

Verim, Aysegul,Turan, Saime,Farooqi, Ammad Ahmad,Kahraman, Ozlem Timirci,Tepe-Karaca, Cigdem,Yildiz, Yemliha,Naiboglu, Baris,Ozkan, Nazli Ezgi,Ergen, Arzu,Isitmangil, Gulbu Aydinoglu,Yaylim, Ilhan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

The laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant tumors occurring in the head and neck. Tumor necrosis factor related apoptosis induce ligand (TRAIL) and TRAIL-receptors (DR4, DR5, DcR1, DcR2) are known as important members of TRAIL-mediated biochemical signaling pathway. Associations between polymorphisms in these genes and clinicopathological characteristics of human laryngeal carcinoma are not well defined. This study therefore aimed to investigate a possible relationship among the TRAIL and TRAIL-DR4 polymorphisms and sTRAIL levels in the risk or progression of LSCC. A total of 99 patients with laryngeal cancer and 120 healthy subjects were enrolled in the study. DR4 C626G and TRAIL 1595 C/T genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and sTRAIL levels were measured by ELISA. There were significant differences in the distribution of DR4 C626G genotypes and frequencies of the alleles between laryngeal cancer patients and controls (p<0.001) but not in TRAIL 1595 C/T. We found the increased frequency of the DR4 C626G homozygote CC genotype in patients than in controls (p<0.001). Haplotype analysis revealed that there was also a statistically significant relationship between TRAIL and TRAIL-DR4 polymorphisms and laryngeal cancer. Serum sTRAIL levels in the laryngeal patients with CC genotype who had advanced tumour stage were lower than those of patients with early tumor stage (p=0.014). Our findings suggest that DR4 C626G genotypes and sTRAIL levels might be associated with progression of laryngeal cancer in the Turkish population.

Essential Oil Composition and Antioxidant Activities of Alkanet (Alkanna tinctoria subsp. tinctoria)

M. Sabih Ozer,Cengiz Sarikurkcu,Sendil Can,Bektas Tepe 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.5

This study is designed to examine the chemical composition and in vitro antioxidant activity of the hydrodistillated essential oil and the various extracts of alkanet (Alkanna tinctoria subsp. tinctoria). Gas chromatography (GC) and GC-mass spectrometry (GC-MS) analysis of the essential oil were resulted in the determination of 27different compounds, representing 93.32% of the total oil. The major compounds detected in the oil, were pulegone (22.27%), 1,8-cineole (13.03%), α-terpinyl acetate (6.87%),and isophytol (6.83%), respectively. Antioxidant activities of the samples were determined by 4 different test systems namely β-carotene/linoleic acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power, and chelating effect. In all systems, essential oil showed the weakest activity profile. On the other hand, ethyl acetate and water extracts exhibited excellent antioxidant activities. As well as the antioxidant activities of the extracts, they were evaluated in terms of their total phenolic and flavonoid contents. In parallel to the experiments, ethyl acetate and water extracts were found to be rich-in these phytochemicals.

Evaluation of Antioxidant Activities of 3 Edible Mushrooms: Ramaria flava (Schaef.: Fr.) Quél., Rhizopogon roseolus (Corda) T.M. Fries., and Russula delica Fr.

Nevcihan Gursoy,Cengiz Sarikurkcu,M. Halil Solak,Bektas Tepe 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.3

The methanolic extracts of Ramaria flava,Rhizopogon roseolus, and Russula delica were analyzed for their antioxidant activities in different test systems including β-carotene/linoleic acid, 1.1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, reducing power, and metal chelating activities in addition to their total phenolic and flavonoid contents. In the first case,methanol extract of R. roseolus showed the strongest activity. In DPPH system, the scavenging effects increased with the concentration. The reducing power of the mushroom also increased with concentration. Chelating effect was 96.75±0.28% for R. flava. In the case of total phenolic and flavonoid assays, R. flava found to have the highest phenolic content. Total flavonoid content of R. flava again found the superior to the other mushrooms. Experimental results indicate that the mushroom species evaluated here can be consumed safely. On the other hand, knowing the biological activity of these mushrooms will contribute to the establishment of conscious consumption.

Phenolic composition, enzyme inhibitory, and antioxidant activity of Bituminaria bituminosa

Sarikurkcu, Cengiz,Cengiz, Mustafa,Uren, Mehmet Cemil,Ceylan, Olcay,Orenc, Tuba,Tepe, Bektas 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

This study aimed to evaluate the in vitro antioxidant and enzyme inhibitory activities of ethyl acetate, methanol, and water extracts of Bituminaria bituminosa. In phosphomolybdenum assay, the methanol extract showed the highest activity ($166.78{\mu}mol\;TEs/g$ dry plant). The water extract exhibited the highest scavenging activity on 1,1-diphenyl-2-picrylhydrazyl ($DPPH^{\bullet}$) and 2,2-azino-bis (3-ethylbenzothiazloine-6-sulphonic acid) ($ABTS^{{\bullet}+}$). In addition, it exhibited the highest activity in cupric ion reducing (CUPRAC) and ferric reducing antioxidant power (FRAP) assays (41.26 and $46.82{\mu}mol\;TEs/g$ dry plant). The extracts did not show cholinesterase and tyrosinase inhibitory activity. However, ${\alpha}$-glucosidase inhibition assay resulted in the superiority of water extract ($1233.86{\mu}mol\;ACEs/g$ dry plant). In the case of ${\alpha}$-amylase inhibitory assay, the ethyl acetate extract showed the highest activity ($53.65{\mu}mol\;ACEs/g$ dry plant). The water extract exhibited the highest phenolic content ($31.70{\mu}mol\;GAEs/g$ dry plant). In contrast, the methanol extract was found rich in flavonoid compounds ($5.29{\mu}mol\;REs/g$ dry plant). The water extract contained considerable amounts of rosmarinic acid, luteolin, quercetin, and rutin. Therefore, it can be used as a source of new and alternative antioxidant and enzyme inhibitory agents.