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V771TAU의채층활동 한국우주과학회 1997 Journal of Astronomy and Space Sciences Vol.14 No.1
V711 Tau의 채층활동을 조사하기 위하여 자외선 영역의 Mg II 방출선의 세기와 광도 변화의 상관 관계를 유추하였다. 첫째, 가시광선 영역의 광도곡선의 형태와 자외선 영역의 광도곡선의 형태를 비교하고, 둘째, IUE 고분산 스펙트럼에서 Mg II 방출선의 세기를 위상별로 측정하고 방출선 부근의 연속선을 이용하여 자외선 광도곡선을 완성하였다. 방출선의 세기와 광도곡선을 비교한 결과 방출선이 강하게 나타나는 위상, $0.^{p}4$에서는 광도가 어둡게 나타나 최소가 되고, 방출선의 세기가 약할 때는 광도가 밝아져서 최대가 되었다. 이는 자기장이 강한 위상에서는 채층활동과 흑점의 크기가 증가하고 반대로 자기장이 약한 위상에서는 채층활동과 흑점의 크기가 작다는 것을 뜻한다. 그러므로 자기장, 채층활동, 별 표면의 흑점과의 상관 관계가 밝혀지고, V711 Tau의 변광은 표면의 채층활동과 흑점에 의한 것이다. The relationship between Mg II emission line and light variation of V711 Tau has been derived to investigate the chromospheric activity on V711 Tau. First, a shape of an optical light curve was compared with that of ultraviolet constructed from the IUE low resolution spectra. Second, the intensities of Mg II k emission lines have been reduced from IUE high resolution spectra. The intensity of Mg II k line was compared with brightness of the UV light curve at given phase. The Mg II line intensity is maximum at the phase $O.^{P}4$ where the light is minimum. The evidence of chromosperic activity is indicated by the intensity variation of the MgII emission line with orbital phase for V711 Tau.
Purification of Filamentous Bacteriophage M13 by Expanded Bed Anion Exchange Chromatography
Tau Chuan Ling,Chee Kin Loong,Wen Siang Tan,Beng Ti Tey,Wan Mohammad Wan Abdullah,Arbakariya Ariff 한국미생물학회 2004 The journal of microbiology Vol.42 No.3
In this paper, we investigated the development of a simplified and rapid primary capture step for the recovery of M13 bacteriophage from particulate-containing feedstock. M13 bacteriophage, carrying an insert, was propagated and subsequently purified by the application of both conventional multiple steps and expanded bed anion exchange chromatography. In the conventional method, precipitation was conducted with PEG/NaCl, and centrifugation was also performed. In the single step expanded bed anion exchange adsorption, UpFront FastLineTM20 (20 mm i.d.) from UpFront Chromatography was used as the contactor, while 54 ml (Ho=15cm) of STREAMLINE DEAE (ρ=1.2 g/cm3) from Amersham Pharmacia Biotechnology was used as the anion exchanger. The performance of the two methods were evaluated, analysed, and compared. It was demonstrated that the purification of the M13 bacteriophage, using expanded bed anion exchange adsorption, yielded the higher recovery percentage, at 82.86%. The conventional multiple step method yielded the lower recovery percentage, 36.07%. The generic application of this integrated technique has also been assessed.
Tau Chuan Ling,Yen Mei Chow,Beng Ti Tey,Mohd Nordin Ibrahim,Arbakariya Ariff 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.6
The influence of whole yeast cells (0~15% w/v) on the protein adsorption performance in dye-ligand chromatography was explored. The adsorption of a model protein, bovine serum albumin (BSA), was selected to demonstrate this approach. The UpFront adsorbent (ρ = 1.5 g/cm3) derivatised with Cibacron Blue 3GA and a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography, Denmark, were employed in the batch binding and expanded bed operation. The BSA binding capacity was demonstrated to not be adversely affected by the presence of yeast cells. The dy-namic binding capacity of BSA at a C/C0 = 0.1 biomass concentration of 5, 10, 15% w/v were 9, 8, and 7.5 mg/mL of settled adsorbent, respectively.
Tau Chuan Ling,Yen Mei Chow,Beng Ti Tey,Mohd Nordin Ibrahim,Arbakariya Ariff 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.3
A dense, pellicular UpFront adsorbent (ρ = 1.5 g/cm3, UpFront Chromatography, Cophenhagen, Denmark) was characterized in terms of hydrodynamic properties and protein adsorption performance in expanded bed chromatography. Cibacron Blue 3GA was immobilised into the adsorbent and protein adsorption of bovine serum albumin (BSA) was selected to test the setup. The Bodenstein number and axial dispersion coefficient estimated for this dense pellicular adsorbent was 54 and 1.63 × 10-5 m2/s, respectively, indicating a stable expanded bed. It could be shown that the BSA protein was captured by the adsorbent in the presence of 30% (w/v) of whole-yeast cells with an estimated dynamic binding capacity (C/Co = 0.01) of approximately 6.5 mg/mL adsorbent.
Effect of Polymer Shielding on Elution of G3PDH Bound to Dye-ligand Adsorbent
Tau Chuan Ling,Andrew Lyddiatt 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.1
Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone, PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency, at 6.5% and a specific activity of 10.2 IU/mg.
Effect of Polymer Shielding on Elution of G3PDH Bound to Dye-ligand Adsorbent
Ling Tau Chuan,Lyddiatt Andrew The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.1
Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone. PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency. at 6.5% and a specific activity of 10.2 IU/mg.
Huei-Tau Ouyang,Kue-Hong Chen,Chi-Ming Tsai 대한조선학회 2015 International Journal of Naval Architecture and Oc Vol.7 No.6
The water wave characteristics of Bragg reflections from a train of fixed floating pontoon breakwaters was studied numerically. A numerical model of boundary discretization type was developed to calculate the wave field. The model was verified by comparing to analytical data in literature and good agreements were achieved. Series of parametric studies were conducted systematically to investigate the dependence of the reflected coefficients by the Bragg scattering on the design variables, including the spacing between the breakwaters, the total number of installed breakwaters, the draft and width do the breakwater, and wave length. Certain wave characteristics of the Bragg reflections were observed and discussed in details which might be of help for practical engineering applications in shoreline protection from incident waves.