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Cassia Angustifolia Primed ASCs Accelerate Burn Wound Healing by Modulation of Inflammatory Response
Tasneem Saba,Ghufran Hafiz,Azam Maryam,Arif Amna,Bin Umair Musab,Yousaf Muhammad Amin,Shahzad Khurrum,Mehmood Azra,Malik Kausar,Riazuddin Sheikh 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.1
Background: Thermal traumas impose a huge burden on healthcare systems. This merits the need for advanced but cost-effective remedies with clinical prospects. In this context, we prepared a regenerative 3D-construct comprising of Cassia angustifolia extract (SM) primed adipose-derived stem cells (ASCs) laden amniotic membrane for faster burn wound repair. Methods: ASCs were preconditioned with SM (30 µg/ml for 24 h), and subsequently exposed to in-vitro thermal injury (51 °C,10 min). In-vivo thermal injury was induced by placing pre-heated copper-disc (2 cm diameter) on dorsum of the Wistar rats. ASCs (2.0 × 105) pre-treated with SM (SM-ASCs), cultured on stromal side of amniotic membrane (AM) were transplanted in rat heat-injury model. Non-transplanted heat-injured rats and non-heat-injured rats were kept as controls. Results: The significantly upregulated expression of IGF1, SDF1A, TGFβ1, VEGF, GSS, GSR, IL4, BCL2 genes and downregulation of BAX, IL6, TNFα, and NFkB1 in SM-ASCs in in-vitro and in-vivo settings confirmed its potential in promoting cell-proliferation, migration, angiogenesis, antioxidant, cell-survival, anti-inflammatory, and wound healing activity. Moreover, SM-ASCs induced early wound closure, better architecture, normal epidermal thickness, orderly-arranged collagen fibers, and well-developed skin appendages in healed rat-skin transplanted with AM+SM-ASCs, additionally confirmed by increased expression of structural genes (Krt1, Krt8, Krt19, Desmin, Vimentin, α-Sma) in comparison to untreated-ASCs laden-AM transplanted in heat injured rats. Conclusion: SM priming effectively enabled ASCs to counter thermal injury by significantly enhancing cell survival and reducing inflammation upon transplantation. This study provides bases for development of effective combinational therapies (natural scaffold, medicine, and stem cells) with clinical prospects for treating burn wounds. Background: Thermal traumas impose a huge burden on healthcare systems. This merits the need for advanced but cost-effective remedies with clinical prospects. In this context, we prepared a regenerative 3D-construct comprising of Cassia angustifolia extract (SM) primed adipose-derived stem cells (ASCs) laden amniotic membrane for faster burn wound repair. Methods: ASCs were preconditioned with SM (30 µg/ml for 24 h), and subsequently exposed to in-vitro thermal injury (51 °C,10 min). In-vivo thermal injury was induced by placing pre-heated copper-disc (2 cm diameter) on dorsum of the Wistar rats. ASCs (2.0 × 105) pre-treated with SM (SM-ASCs), cultured on stromal side of amniotic membrane (AM) were transplanted in rat heat-injury model. Non-transplanted heat-injured rats and non-heat-injured rats were kept as controls. Results: The significantly upregulated expression of IGF1, SDF1A, TGFβ1, VEGF, GSS, GSR, IL4, BCL2 genes and downregulation of BAX, IL6, TNFα, and NFkB1 in SM-ASCs in in-vitro and in-vivo settings confirmed its potential in promoting cell-proliferation, migration, angiogenesis, antioxidant, cell-survival, anti-inflammatory, and wound healing activity. Moreover, SM-ASCs induced early wound closure, better architecture, normal epidermal thickness, orderly-arranged collagen fibers, and well-developed skin appendages in healed rat-skin transplanted with AM+SM-ASCs, additionally confirmed by increased expression of structural genes (Krt1, Krt8, Krt19, Desmin, Vimentin, α-Sma) in comparison to untreated-ASCs laden-AM transplanted in heat injured rats. Conclusion: SM priming effectively enabled ASCs to counter thermal injury by significantly enhancing cell survival and reducing inflammation upon transplantation. This study provides bases for development of effective combinational therapies (natural scaffold, medicine, and stem cells) with clinical prospects for treating burn wounds.
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Naeemullah,Tasneem Gul Kazi,Mustafa Tuzen 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.35 No.-
A novel, simple and rapid single and multi-step cloud point extraction procedure was developed for theselective and simultaneous preconcentration of silver, cadmium and nickel in real samples prior to theirdetermination by flame atomic absorption spectrometry. Dithizone was used as neutral hydrophobiccomplexing agent. Separation of understudy metals can be achieved by controlling the pH value of theextraction system. Evaluation of the two CPE methods was checked by paired t-test, which indicated anon-significant difference. It was successfully applied to the real water sample. Certified referencematerials were used for the accuracy of the green extraction methods. The regression equation for thecalibration curve was linear in the range of 10–150 mg L 1 for Ag, 5–100 mg L 1 for Cd, 10–200 mg L 1 forNi. Relative standard deviation (RSD%) values were found to be lower than 2.5% for all elements. Enhancement factor (EF) and limit of detection (LOD) values were found in the range of 41–58 and 0.27–1.12 mg L 1, respectively.
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Muhammad Bilal,Tasneem G. Kazi,Hassan I. Afridi,Mohammad Balal Arain,Jameel A. Baig,Mustafa Khan,Naeemullah Khan 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.40 No.-
A new method based on modification of cloud point extraction for simultaneous enrichment ofcadmium, lead and copper in water and fish muscle samples was proposed. The extraction efficiency bymodified cloud point extraction (m-CPE) was compared with conventional cloud point extraction (c-CPE)method. The procedure for both CPE methods was comprised of formation of metal complexes with ahydrophobic chelating agent, dithizone, followed by entrapment of the chelates in a nonionic surfactant,Triton X-114. For c-CPE, the surfactant rich phase was treated with ethanolic solution of nitric acid andanalyzed by flame atomic absorption spectrometer (FAAS). Whereas for m-CPE, aqueous nitric acid wasused to back extract the metal ions from the surfactant rich phase and finally determined. The efficiencyof the methods was tested by analyzing certified reference material and standard addition to a realsample. All the experimental parameters were optimized. At optimized experimental conditions,preconcentration and enhancement factors were 62.5 and 78.0 to 83.0 respectively for m-CPE, 25.0 and56.0% higher than that of c-CPE. This improvement might be due to elimination of the effects ofsurfactant on the signal of analytes by FAAS. The developed method of m-CPE was applied successfullyfor analysis of the selected heavy metals in water and muscle tissues samples of fish of different lakes inSindh, Pakistan.
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Sumaira Khan,Tasneem Gul Kazi,Mustafa Soylak 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.27 No.-
An in-syringe ionic liquid-based single step microextraction has been analytically presented. 1-butyl-3-methylimidazolium hexafluorophosphate as extractant and Triton X-100 as dispersing medium wereused. Cd-Dithizone was extracted within a syringe. The effects of some variables such as [C4mim][PF6]volume, pH, TX-100 volume, dithizone concentration, incubation time, diluents nature and matrix effectwere investigated. The limit of detection and preconcentration factor were found as 0.35 mg L 1 and 50,respectively. The validity was checked by using certified reference materials. The relative standarddeviation (RSD) was 4.2%. The method was satisfactorily applied to preconcentration of cadmium innatural water samples.
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Asma Akhtar,Tasneem Gul Kazi,Hassan Imran Afridi,Mustafa Khan,Muhammad Bilal,Noman Khan 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.59 No.-
A modified cloud point extraction method (m-CPE) was developed for the speciation of chromium species (Cr3+ and Cr6+) in artificial saliva extracts (ASE) of snuff products. In this method, Cr3+ was complexed with 8-hydroxyquinoline, which was trapped in nonionic surfactant (Triton X-114), prior to analyzed by electrothermal atomic absorption spectrometer (ETAAS). Whereas, the total extractable Cr was determined by reducing Cr6+ to Cr3+ using Na2SO3 as a reducing reagent. Several parameters were optimized for the developed m-CPE. Under the most favorable conditions, enrichment factor, enhancement factor and limit of detection for the proposed method were 60, 134 and 0.04 μg L−1, respectively having relative standard deviation <5%. Health risks associated with the intake of total Cr in snuff products was also assessed. Estimated daily intake of Cr via sniffing 10 g/day of dry and moist snuff products was found below the maximum tolerable daily intake whereas the calculated risk for cancer due to Cr was observed in the acceptable range of 10 E−6–E−4.
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Faheem Shah,Naeemullah,Tasneem Gul Kazi,Rafaqat Ali Khan,Murtaza Sayed,Hassan Imran Afridi,Khizar Hussain Shah,Jan Nisar 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.48 No.-
A novel restricted access sorbents based micro solid phase extraction (RAS-mSPE) for the extraction ofheavy metal from biological samples has been developed. Cadmium (Cd) and manganese (Mn) weredirectly extracted skipping the tedious, time consuming and expensive sample preparation stepexcluding all of the existing proteins. Sorbent’s (activated carbon cloths) surface was modified withbovine serum albumin through glutaraldehyde to make restricted access sorbent (RAS). Differentvariables affecting the extraction efficiency were selected for optimization. The limits of detectionobtained for Cd and Mn were 0.252 and 0.214 mg L 1, respectively. Analyte recoveries in fortified humanwhole blood serum and milk samples were found in the range of 90.3–103.9%. The procedure waseffectively used for Cd and Mn extraction in real samples devoid of any pretreatment step.
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Sadaf S. Arain,Tasneem G. Kazi,Hassan I. Afridi,Naeemullah,Kapil D. Brahman,Muhammad B. Arain,Abdul H. Panhwar,Mariam S. Arain,Shahnawaz Baloch 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.34 No.-
A green non-dispersive ionic liquid based microextraction (ND-ILmE) method was proposed for thepreconcentration of trace levels of copper (Cu2+) in artificial saliva extract (ASE) of smokeless tobaccoproducts (SLT). A 100 cm long, narrow glass column containing aqueous solution of Cu/ASE was used toincrease phase transfer ratio by providing more contact area between two medium, which drasticallyimprove the recovery of Cu-PAN complex into ionic liquid 1-butyl-3-methylimidazolium hexafluor-ophosphate [C4mim][PF6]. At optimum experimental conditions, the detection limit and theenhancement factor were found to be 0.042 mg L 1 and 85, respectively. This method was successfullyapplied to real samples.
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Design and synthesis of non-steroidal diclofenac derivatives as anti-inflammatory drugs
Tae-Woo Kim,Tasneem Islam,Kang-Yeoun Jung 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.3
In the course of our studies directed towards the discovery of novel non-steroidal anti-inflammatory agents (NSAIDs), which are expected to have improved drug efficacy and better toxicity profile than diclofenac, we have synthesized new derivatives of diclofenac phosphonates. These phosphonate analogs were efficiently prepared from commercially available 2-hydroxymethylaniline in three steps. The 2-hydroxymethylaniline was converted to the azide in reasonable yield. Click chemistry with a variety of alkynes followed by Arbuzov reaction resulted in a series of triazole based phosphonate products. Our inhibitors are designed to reduce the gastrointestinal bleeding caused by diclofenac by substituting the carboxylic acid group with the phosphonate group and in addition replacing with the triazole ring would allow the flexibility to explore the importance of both hydrophilicity and hydrophobicity of the ring at the active site. 2010 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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